Research

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Joshua Mauney, PhD

Josh Mauney, PhD
Department:
Urology Research
Hospital Title:
Staff Scientist
Academic Title:
Assistant Professor of Surgery, Harvard Medical School
Research Focus Area:
Tissue EngineeringUrinary and Gastrointestinal Tract Surgical ResearchBiomaterials
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Research Overview

My research focuses on the development of novel tissue engineering strategies for hollow organ repair and regeneration. Specifically, I investigate the utility of silk scaffolds as platforms for urogenital, gastrointestinal, and respiratory tissue reconstruction. By deciphering how variations in scaffold processing parameters can affect the in vivo performance of silk biomaterials, we seek to develop 3-D matrix designs with optimal structural, mechanical, and degradation profiles capable of restoring function in defects associated with traumatic injury and fibrotic diseases. In collaboration with our clinical partner, Dr. Carlos Estrada, MD, we employ both small and large animal models of tissue repair in the bladder, urethra, small intestine, esophagus, and trachea. In addition, my laboratory explores the mechanisms of constructive remodeling of tissue engineered constructs by delineating how host tissue microenvironments interact, populate, and integrate into scaffold environments in vivo. Our overall goal is the creation of clinically-useful scaffold configurations for hollow organ regeneration by engineering materials which fulfill structural and mechanical requirements of native tissues as well as present microenvironmental cues necessary for host tissue integration and defect consolidation. My comprehensive background in silk-based biomaterial fabrication methods coupled with my expertise in stem cell biology uniquely positions my lab as a pioneer in the creation of novel strategies for hollow organ regeneration.

Key Publications

Tu DD, Chung YG, Gil ES, Seth A, Franck D, Cristofaro V, Sullivan MP, Di Vizio D, Gomez P 3rd, Adam RM, Kaplan DL, Estrada CR Jr, Mauney JR. Bladder tissue regeneration using acellular bi-layer silk scaffolds in a large animal model of augmentation cystoplasty. Biomaterials. 2013 Nov;34(34):8681-9. PMID: 23953839.

Chung YG, Tu D, Franck D, Gil ES, Algarrahi K, Adam RM, Kaplan DL, Estrada CR Jr, Mauney JR. Acellular bi-layer silk fibroin scaffolds support tissue regeneration in a rabbit model of onlay urethroplasty. PLoS One. 2014 Mar 14;9(3):e91592. PMID: 24632740.

Chung YG, Algarrahi K, Franck D, Duong DD, Adam RM, Kaplan DL, Estrada CR, Mauney JR. The Use of Bi-layer Silk Fibroin Scaffolds and Small Intestinal Submucosa Matrices to Support Bladder Tissue Regeneration in a Rat Model of Spinal Cord Injury. 2014 Biomaterials. Aug;35(26):7452-9. PMID: 24917031.

Algarrahi K, Franck D, Ghezzi C, Cristafaro V, Yang X, Sullivan MP, Chung YG, Affas S, Jennings R, Kaplan DL, Estrada CR, Mauney JR. Acellular Bi-Layer Silk Fibroin Scaffolds Support Functional Tissue Regeneration in a Rat Model of Onlay Esophagoplasty. Biomaterials. 2015 Jun;53:149-59.2015. PMID: 25890715

See a complete list of published work in my bibliography.

Publications

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  1. Sack BS, Mauney JR, Estrada CR. Silk Fibroin Scaffolds for Urologic Tissue Engineering. Curr Urol Rep. 2016 Feb; 17(2):16.
  2. Chung YG, Seth A, Doyle C, Franck D, Kim D, Cristofaro V, Benowitz LI, Tu DD, Estrada CR, Mauney JR, Sullivan MP, Adam RM. Inosine Improves Neurogenic Detrusor Overactivity following Spinal Cord Injury. PLoS One. 2015; 10(11):e0141492.
  3. Algarrahi K, Franck D, Ghezzi CE, Cristofaro V, Yang X, Sullivan MP, Chung YG, Affas S, Jennings R, Kaplan DL, Estrada CR, Mauney JR. Acellular bi-layer silk fibroin scaffolds support functional tissue regeneration in a rat model of onlay esophagoplasty. Biomaterials. 2015 Jun; 53:149-59.
  4. Franck D, Chung YG, Coburn J, Kaplan DL, Estrada CR, Mauney JR. In vitro evaluation of bi-layer silk fibroin scaffolds for gastrointestinal tissue engineering. J Tissue Eng. 2014; 5:2041731414556849.
  5. Mauney JR, Adam RM. Dynamic reciprocity in cell-scaffold interactions. Adv Drug Deliv Rev. 2015 Mar; 82-83:77-85.
  6. Chung YG, Algarrahi K, Franck D, Tu DD, Adam RM, Kaplan DL, Estrada CR, Mauney JR. The use of bi-layer silk fibroin scaffolds and small intestinal submucosa matrices to support bladder tissue regeneration in a rat model of spinal cord injury. Biomaterials. 2014 Aug; 35(26):7452-9.
  7. Chung YG, Tu D, Franck D, Gil ES, Algarrahi K, Adam RM, Kaplan DL, Estrada CR, Mauney JR. Acellular bi-layer silk fibroin scaffolds support tissue regeneration in a rabbit model of onlay urethroplasty. PLoS One. 2014; 9(3):e91592.
  8. Gandhi D, Molotkov A, Batourina E, Schneider K, Dan H, Reiley M, Laufer E, Metzger D, Liang F, Liao Y, Sun TT, Aronow B, Rosen R, Mauney J, Adam R, Rosselot C, Van Batavia J, McMahon A, McMahon J, Guo JJ, Mendelsohn C. Retinoid signaling in progenitors controls specification and regeneration of the urothelium. Dev Cell. 2013 Sep 16; 26(5):469-82.
  9. Tu DD, Chung YG, Gil ES, Seth A, Franck D, Cristofaro V, Sullivan MP, Di Vizio D, Gomez P, Adam RM, Kaplan DL, Estrada CR, Mauney JR. Bladder tissue regeneration using acellular bi-layer silk scaffolds in a large animal model of augmentation cystoplasty. Biomaterials. 2013 Nov; 34(34):8681-9.
  10. Seth A, Chung YG, Gil ES, Tu D, Franck D, Di Vizio D, Adam RM, Kaplan DL, Estrada CR, Mauney JR. The performance of silk scaffolds in a rat model of augmentation cystoplasty. Biomaterials. 2013 Jul; 34(20):4758-65.
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  12. Franck D, Gil ES, Adam RM, Kaplan DL, Chung YG, Estrada CR, Mauney JR. Evaluation of silk biomaterials in combination with extracellular matrix coatings for bladder tissue engineering with primary and pluripotent cells. PLoS One. 2013; 8(2):e56237.
  13. Tu DD, Seth A, Gil ES, Kaplan DL, Mauney JR, Estrada CR. Evaluation of biomaterials for bladder augmentation using cystometric analyses in various rodent models. J Vis Exp. 2012 Aug 09; (66).
  14. DeGraff DJ, Cates JM, Mauney JR, Clark PE, Matusik RJ, Adam RM. When urothelial differentiation pathways go wrong: implications for bladder cancer development and progression. Urol Oncol. 2013 Aug; 31(6):802-11.
  15. Mauney JR, Cannon GM, Lovett ML, Gong EM, Di Vizio D, Gomez P, Kaplan DL, Adam RM, Estrada CR. Evaluation of gel spun silk-based biomaterials in a murine model of bladder augmentation. Biomaterials. 2011 Jan; 32(3):808-18.
  16. Mauney J, Olsen BR, Volloch V. Matrix remodeling as stem cell recruitment event: a novel in vitro model for homing of human bone marrow stromal cells to the site of injury shows crucial role of extracellular collagen matrix. Matrix Biol. 2010 Oct; 29(8):657-63.
  17. Mauney JR, Ramachandran A, Yu RN, Daley GQ, Adam RM, Estrada CR. All-trans retinoic acid directs urothelial specification of murine embryonic stem cells via GATA4/6 signaling mechanisms. PLoS One. 2010 Jul 13; 5(7):e11513.
  18. Mauney J, Volloch V. Adult human bone marrow stromal cells regulate expression of their MMPs and TIMPs in differentiation type-specific manner. Matrix Biol. 2010 Jan; 29(1):3-8.
  19. Mauney J, Volloch V. Human bone marrow-derived stromal cells show highly efficient stress-resistant adipogenesis on denatured collagen IV matrix but not on its native counterpart: implications for obesity. Matrix Biol. 2010 Jan; 29(1):9-14.
  20. Mauney J, Volloch V. Progression of human bone marrow stromal cells into both osteogenic and adipogenic lineages is differentially regulated by structural conformation of collagen I matrix via distinct signaling pathways. Matrix Biol. 2009 Jun; 28(5):239-50.
  21. Mauney J, Volloch V. Collagen I matrix contributes to determination of adult human stem cell lineage via differential, structural conformation-specific elicitation of cellular stress response. Matrix Biol. 2009 Jun; 28(5):251-62.
  22. Moreau JE, Anderson K, Mauney JR, Nguyen T, Kaplan DL, Rosenblatt M. Tissue-engineered bone serves as a target for metastasis of human breast cancer in a mouse model. Cancer Res. 2007 Nov 1; 67(21):10304-8.
  23. Mauney JR, Nguyen T, Gillen K, Kirker-Head C, Gimble JM, Kaplan DL. Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds. Biomaterials. 2007 Dec; 28(35):5280-90.
  24. Moreau J, Anderson KM, Mauney JR, Kaplan D, Rosenblatt M. Studies of osteotropism on both sides of the breast cancer-bone interaction. Ann N Y Acad Sci. 2007 Nov; 1117:328-44.
  25. Mauney JR, Kirker-Head C, Abrahamson L, Gronowicz G, Volloch V, Kaplan DL. Matrix-mediated retention of in vitro osteogenic differentiation potential and in vivo bone-forming capacity by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion. J Biomed Mater Res A. 2006 Dec 1; 79(3):464-75.
  26. Mauney JR, Volloch V, Kaplan DL. Matrix-mediated retention of adipogenic differentiation potential by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion. Biomaterials. 2005 Nov; 26(31):6167-75.
  27. Mauney JR, Jaquiéry C, Volloch V, Heberer M, Martin I, Kaplan DL. In vitro and in vivo evaluation of differentially demineralized cancellous bone scaffolds combined with human bone marrow stromal cells for tissue engineering. Biomaterials. 2005 Jun; 26(16):3173-85.
  28. Mauney JR, Volloch V, Kaplan DL. Role of adult mesenchymal stem cells in bone tissue engineering applications: current status and future prospects. Tissue Eng. 2005 May-Jun; 11(5-6):787-802.
  29. Mauney JR, Kaplan DL, Volloch V. Matrix-mediated retention of osteogenic differentiation potential by human adult bone marrow stromal cells during ex vivo expansion. Biomaterials. 2004 Jul; 25(16):3233-43.
  30. Mauney JR, Sjostorm S, Blumberg J, Horan R, O'Leary JP, Vunjak-Novakovic G, Volloch V, Kaplan DL. Mechanical stimulation promotes osteogenic differentiation of human bone marrow stromal cells on 3-D partially demineralized bone scaffolds in vitro. Calcif Tissue Int. 2004 May; 74(5):458-68.
  31. Mauney JR, Blumberg J, Pirun M, Volloch V, Vunjak-Novakovic G, Kaplan DL. Osteogenic differentiation of human bone marrow stromal cells on partially demineralized bone scaffolds in vitro. Tissue Eng. 2004 Jan-Feb; 10(1-2):81-92.
  32. Egles C, Shamis Y, Mauney JR, Volloch V, Kaplan DL, Garlick JA. Denatured collagen modulates the phenotype of normal and wounded human skin equivalents. J Invest Dermatol. 2008 Jul; 128(7):1830-7.
  33. Gomez P, Gil ES, Lovett ML, Rockwood DN, Di Vizio D, Kaplan DL, Adam RM, Estrada CR, Mauney JR. The effect of manipulation of silk scaffold fabrication parameters on matrix performance in a murine model of bladder augmentation. Biomaterials. 2011 Oct; 32(30):7562-70.
  34. Kim J, Ji M, DiDonato JA, Rackley RR, Kuang M, Sadhukhan PC, Mauney JR, Keay SK, Freeman MR, Liou LS, Adam RM. An hTERT-immortalized human urothelial cell line that responds to anti-proliferative factor. In Vitro Cell Dev Biol Anim. 2011 Jan; 47(1):2-9.
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