Molecular Genetics Core | Equipment

Name Make/Model Functionality Location
Fluidigm Biomark high performance PCR system Fluidigm/Biomark Microfluidics technology to process samples at nanoliter-scale volumes for gene expression, genotyping, sample identification, copy number variation analysis and digital PCR. Ideal for labs performing PCR on hundreds to thousands of samples. Provides a wide range of integrated fluidic circuit (IFC) formats available, scaling from mid- to high throughput on a single system is easily achievable. Available controller are HX, MX, and RX. CLS16- 16030.21
BIORAD Droplet Digital PCR System BIORAD/QX200 with Auto DG The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
Most precise and sensitive digital PCR solution for a wide variety of applications.
CLS16- 16030.22
Perkin Elmer Multilabel Counter Perkin Elmer/Victor 3 Fluorescence detection for quantification using Picogreen or Ribogreen. CLS16- 16030.22
Agilent Bioanalyzer
Agilent/Bioanlyzer 2100 The 2100 Bioanalyzer system is an established automated electrophoresis tool for the sample quality control of biomolecules. The 2100 Bioanalyzer instrument, together with the 2100 Expert Software and Bioanalyzer assays, provide highly precise analytical evaluation of various samples types in many workflows, including next generation sequencing (NGS), gene expression, biopharmaceutical, and gene editing research. Digital data is provided in a timely manner and delivers objective assessment of sizing, quantitation, integrity and purity from DNA, RNA, and proteins. Minimal sample volumes are required for an accurate result, and the data may be exported in a many different formats for ease-of-use.  CLS16- 16030.23
QIAGEN Automated Nucleic Acid Extraction QIAGEN/QIAcube For fully automated nucleic acid extraction with QIAGEN spin-column kits.
Automates over 80 QIAGEN kits with over 3000 protocols. Allows automated DNA, RNA and protein sample processing. Eliminates manual processing steps. Generates standardized results. 
CLS16- 16030.23 
Agilent Microarray Scanner
Agilent/SureScan The Agilent SureScan Scanner provides a precise and efficient microarray scanner system for many research applications. Designed for research using microarrays, the scanner system provides a laser-induced fluorescence scanning solution designed to read microarrays printed on standard 1 inch by 3 inch glass slides. The microarray instrumentation measures the fluorescence intensity of two dyes simultaneously from labeled sample nucleic acid bound to microarrays. The system also enhances productivity with continuous loading from a 24-slide cassette and automatic loading of data files into Feature Extraction Software. It also features built-in ozone protection to minimize signal degradation.  CLS16- 16030.23 
GeneChip Microarray Scanner Thermo Fisher/3000 7G Scan next-generation higher-density arrays, including SNP arrays with up to 900,000 SNPs, tiling arrays for transcription and all-exon arrays for whole-genome analysis. CLS16- 16030.24
Nanostring nCounter® SPRINT Profiler   Nanostring/Sprint Profiler The nCounter® SPRINT Profiler makes access to NanoString’s molecular barcoding technology more affordable than ever before for RNA, DNA, and protein profiling applications. Digitally examine multiple pathways in a single tube in an extraction-free workflow. Perform your own data analysis using the nSolver™ Analysis Software. Compatible with difficult sample types, including FFPE tissue. Highly multiplexed analysis of basic cancer biology and pathway deregulation activity.  CLS16- 16049
Bionano Saphyr
Bionano/Saphyr
The Saphyr System by Bionano Genomics is a whole genome imaging tool for high-speed, high-throughput structural variant detection and analysis with exceptional sensitivity and specificity. Wide range of applications includes: undiagnosed genetic disorders, hematologic malignancies, gene discovery and therapy, cell line stability, solid tumor research, genetic engineering studies, evolutionary biology, and reference genome assembly.  Resolve large-scale structural variations missed by next-generation sequencing systems. Detect structural variants with high specificity and sensitivity.
CLS16- 16049
ABI 3730 DNA Analyzers
Thermo Fisher/SeqStudio The 48-capillary 3730 DNA Analyzer is the Gold Standard in medium-to-high throughput genetic analysis. Use the 3730 DNA Analyzer for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample. Two are available.
CLS16- 16030.21
CFX 96 and CFX384 Touch Real-Time PCR Detection System
BIORAD/CFX96 The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR. CLS16- 16054
CFX384 Touch Real-Time PCR Detection System BIORAD/CFX384 The CFX384 Touch Real-Time PCR Detection System is a powerful and precise real-time PCR instrument in a 384-well format, for researchers who require both ease of use and rapid, high-throughput data acquisition. The CFX384 Touch System utilizes accurate temperature control and sensitive, 4-color detection optics to deliver reliable and repeatable qPCR results for singleplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful software, the CFX384 Touch System was designed to advance your qPCR. CLS16- 16054
NextSeq 500
Illumina/NextSeq 500 Provides high-quality, high-coverage genome with streamlined library preparation using high-quality TruSeq library preparation kits.  Single, paired-end, and indexed sequencing with accuracy of > 75% of sequenced bases over Q30.  Enables researchers to interrogate simple prokaryotic and complex eukaryotic genomes quickly and efficiently.
CLS16- 16054
SeqStudio Genetic Analyzer System Applied Biosystems/SeqStudio  Capillary electrophoresis system capable of Sanger Sequencing analysis (for NGS confirmation, indel detection, SNP detection, genome editing verification, microbial identification, etc…) and fragment analysis (ie microsatellite analysis).  CLS16- 16030.21
NanoDrop 8000 Spectrophotometer
ThermoFisher/NanoDrop 8000  Microvolume UV-Vis and Fluorescence spectrophotometer used for nucleic acid and protein quantification and quality assessment. Additionally capable of Microarray fluorescent labeling QC analysis, as well as Pierce 660, Bradford, BCA, Lowry assays. 8000 model has 8 channels improving speed. 
CLS16- 16030.23
4200 TapeStation
Agilent/4200 TapeStation
Fully automated electrophoresis device for RNA and DNA sample quality assessment. Capable of assessing intactness of extracted genomic DNA or RNA, as well as size and concentration of either nucleic acid. Especially useful for Next-Gen Sequencing quality control, such as library-prep QC. CLS16- 16030.23 
Chromium Controller
10x Genomics/ Chromium Controller
Partitioning device used to isolate single cells from a cell suspension in “Gel Bead-in-Emulsion” droplets, where nucleic acid of interest is extracted and tagged with a cell-specific barcode for downstream Next-Gen Sequencing library prep and sequencing. 
CLS16- 16030.23
nCounter SPRINT Profiler
Nanostring/ SPRINT Profiler
Fully automated analysis platform for Nanostring’s nCounter technology, which allows for molecular quantification of a diverse range of DNA, RNA and protein targets through the use of fluorescent probes. Capable of multiplex analysis of up to 800 targets.
CLS16- 16049