Cell Function and Imaging Core (CFIC)

*The Cell Function and Imaging Core is now open. Please review our new COVID-19 standard operating procedures before booking or arriving to the core.

 

Our mission

The Cell Function and Imaging Core (CFIC) provides state-of-the-art imaging, cell characterization and molecular biology equipment that supports all researchers at Boston Children's Hospital. Our mission is to provide the infrastructure, biologic resources, expertise and training for innovative and cross-disciplinary research in fields of the life sciences, relevant to the health of infants and children, and consistent with the Boston Children's research mission.

Located in the Enders building, CFIC provides access to a wide range of equipment and expertise. Our Imaging equipment range includes laser scanning (LSM 880 Aryscan; Olympus FV3000RS) and spinning disk confocal microscopes, a light sheet microscope, kinetic/high content/high speed automated plate/slide imaging systems (Vala Sciences Kinetic Image Cytometer), and an automated epifluorescent scope capable of live cell imaging (Keyence).

Our cell function equipment includes multi-color flow cytometers, a cell sorter and a Seahorse analyzer.Upon special request and approval Agilent LC and LC Mass Spectrometry and two BioRad FPLC systems are available.

New to the Core next year will be an Abberior STED microscope for fixed and live cell microscopy (anticipated resolution 30-50 nm) and two 10x single cell Chromium Controlers.

 

Lightsheet imaging of human primary TTC7A deficient enteroid stained with actin showing a multiple lumen phenotype. (Thiagarajah Lab)
Lightsheet imaging of human primary TTC7A deficient enteroid stained with actin showing a multiple lumen phenotype (Thiagarajah Lab)