ABOUT THE RESEARCHER

OVERVIEW

Stem cell transplantation and tissue engineering hold great promise in regenerative medicine. However, generating tissues with stable functional vascular beds remains a major challenge. Our laboratory is interested in understanding mechanisms of vascular network formation using human stem and progenitor cells. We specialize in the biology and therapeutic potential of human circulating Endothelial Colony-Forming Cells. Current projects in the lab are focused on:

  • Endothelial colony-forming cells (ECFCs) biology
  • Bioengineering vascular networks
  • Genetically-engineered vasculature for protein drug delivery

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BACKGROUND

Dr. Melero-Martin graduated in Chemical Engineering from University of Seville, Spain, in 1998. After working three years in industry, he went on to earn a PhD in biochemical engineering from the University of Birmingham, UK in 2005. Dr. Melero-Martin's doctoral research focused on tissue engineering and was sponsored by Smith & Nephew. From 2005 to 2008, he trained as a post-doctoral fellow in the Vascular Biology Program at Boston Children's Hospital. He joined the Department of Cardiac Surgery at Children's Hospital in 2008. In 2009, he received a K99/R00 Pathway to Independence Award from the NIH.

PUBLICATIONS

Publications powered by Harvard Catalyst Profiles

  1. Targeting Nanoparticles to Bioengineered Human Vascular Networks. Nano Lett. 2021 Jul 23. View abstract
  2. Local Immunomodulatory Strategies to Prevent Allo-Rejection in Transplantation of Insulin-Producing Cells. Adv Sci (Weinh). 2021 Jul 14; e2003708. View abstract
  3. A nanofibrous encapsulation device for safe delivery of insulin-producing cells to treat type 1 diabetes. Sci Transl Med. 2021 06 02; 13(596). View abstract
  4. Adieu to parting Editor in Chief and pioneering scientist Dr. Joyce Bischoff. Angiogenesis. 2021 May; 24(2):191-193. View abstract
  5. A Biphasic Osteovascular Biomimetic Scaffold for Rapid and Self-Sustained Endochondral Ossification. Adv Healthc Mater. 2021 Jul; 10(13):e2100070. View abstract
  6. Human endothelial colony-forming cells provide trophic support for pluripotent stem cell-derived cardiomyocytes via distinctively high expression of neuregulin-1. Angiogenesis. 2021 May; 24(2):327-344. View abstract
  7. In Vivo Vascular Network Forming Assay. Methods Mol Biol. 2021; 2206:193-203. View abstract
  8. A comprehensive library of human transcription factors for cell fate engineering. Nat Biotechnol. 2021 04; 39(4):510-519. View abstract
  9. Interleukin-8 Receptors CXCR1 and CXCR2 Are Not Expressed by Endothelial Colony-forming Cells. Stem Cell Rev Rep. 2021 Apr; 17(2):628-638. View abstract
  10. Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA. Sci Adv. 2020 Jul; 6(30):eaba7606. View abstract
  11. Illustrated State-of-the-Art Capsules of the ISTH 2020 Congress. Res Pract Thromb Haemost. 2020 Jul; 4(5):680-713. View abstract
  12. Bioengineering hemophilia A-specific microvascular grafts for delivery of full-length factor VIII into the bloodstream. Blood Adv. 2019 12 23; 3(24):4166-4176. View abstract
  13. Standardization of methods to quantify and culture endothelial colony-forming cells derived from peripheral blood: Position paper from the International Society on Thrombosis and Haemostasis SSC. J Thromb Haemost. 2019 Jul; 17(7):1190-1194. View abstract
  14. Bioengineering human vascular networks: trends and directions in endothelial and perivascular cell sources. Cell Mol Life Sci. 2019 Feb; 76(3):421-439. View abstract
  15. Comparison of covalently and physically cross-linked collagen hydrogels on mediating vascular network formation for engineering adipose tissue. Artif Cells Nanomed Biotechnol. 2018; 46(sup3):S434-S447. View abstract
  16. Consensus guidelines for the use and interpretation of angiogenesis assays. Angiogenesis. 2018 08; 21(3):425-532. View abstract
  17. VEGF amplifies transcription through ETS1 acetylation to enable angiogenesis. Nat Commun. 2017 08 29; 8(1):383. View abstract
  18. Host non-inflammatory neutrophils mediate the engraftment of bioengineered vascular networks. Nat Biomed Eng. 2017; 1. View abstract
  19. Elastomeric Fibrous Hybrid Scaffold Supports In Vitro and In Vivo Tissue Formation. Adv Funct Mater. 2017 Jul 19; 27(27). View abstract
  20. Endothelial colony forming cells and mesenchymal progenitor cells form blood vessels and increase blood flow in ischemic muscle. Sci Rep. 2017 04 10; 7(1):770. View abstract
  21. Endothelial Progenitors: A Consensus Statement on Nomenclature. Stem Cells Transl Med. 2017 05; 6(5):1316-1320. View abstract
  22. Devitalized Stem Cell Microsheets for Sustainable Release of Osteogenic and Vasculogenic Growth Factors and Regulation of Anti-Inflammatory Immune Response. Adv Biosyst. 2017 Mar; 1(3). View abstract
  23. Spatiotemporal release of BMP-2 and VEGF enhances osteogenic and vasculogenic differentiation of human mesenchymal stem cells and endothelial colony-forming cells co-encapsulated in a patterned hydrogel. J Control Release. 2016 Feb 10; 223:126-136. View abstract
  24. Bioengineering vascularized tissue constructs using an injectable cell-laden enzymatically crosslinked collagen hydrogel derived from dermal extracellular matrix. Acta Biomater. 2015 Nov; 27:151-166. View abstract
  25. Enzymatic regulation of functional vascular networks using gelatin hydrogels. Acta Biomater. 2015 Jun; 19:85-99. View abstract
  26. Vascular channels formed by subpopulations of PECAM1+ melanoma cells. Nat Commun. 2014 Oct 22; 5:5200. View abstract
  27. Epicardium-to-fat transition in injured heart. Cell Res. 2014 Nov; 24(11):1367-9. View abstract
  28. Microfluidic capture of endothelial colony-forming cells from human adult peripheral blood: phenotypic and functional validation in vivo. Tissue Eng Part C Methods. 2015 Mar; 21(3):274-83. View abstract
  29. TM4SF1: a new vascular therapeutic target in cancer. Angiogenesis. 2014 Oct; 17(4):897-907. View abstract
  30. Human endothelial colony-forming cells serve as trophic mediators for mesenchymal stem cell engraftment via paracrine signaling. Proc Natl Acad Sci U S A. 2014 Jul 15; 111(28):10137-42. View abstract
  31. Decreased level of cord blood circulating endothelial colony-forming cells in preeclampsia. Hypertension. 2014 Jul; 64(1):165-71. View abstract
  32. Maternal body-mass index and cord blood circulating endothelial colony-forming cells. J Pediatr. 2014 Mar; 164(3):566-571. View abstract
  33. Transdermal regulation of vascular network bioengineering using a photopolymerizable methacrylated gelatin hydrogel. Biomaterials. 2013 Sep; 34(28):6785-96. View abstract
  34. Human white adipose tissue vasculature contains endothelial colony-forming cells with robust in vivo vasculogenic potential. Angiogenesis. 2013 Oct; 16(4):735-44. View abstract
  35. Inflamed tumor-associated adipose tissue is a depot for macrophages that stimulate tumor growth and angiogenesis. Angiogenesis. 2012 Sep; 15(3):481-95. View abstract
  36. Equal modulation of endothelial cell function by four distinct tissue-specific mesenchymal stem cells. Angiogenesis. 2012 Sep; 15(3):443-55. View abstract
  37. Functional Human Vascular Network Generated in Photocrosslinkable Gelatin Methacrylate Hydrogels. Adv Funct Mater. 2012 May 23; 22(10):2027-2039. View abstract
  38. Fibroblast growth factor-2 facilitates rapid anastomosis formation between bioengineered human vascular networks and living vasculature. Methods. 2012 Mar; 56(3):440-51. View abstract
  39. Induction of erythropoiesis using human vascular networks genetically engineered for controlled erythropoietin release. Blood. 2011 Nov 17; 118(20):5420-8. View abstract
  40. Thymosin beta 4 treatment after myocardial infarction does not reprogram epicardial cells into cardiomyocytes. J Mol Cell Cardiol. 2012 Jan; 52(1):43-7. View abstract
  41. Bioengineering human microvascular networks in immunodeficient mice. J Vis Exp. 2011 Jul 11; (53):e3065. View abstract
  42. Adult mouse epicardium modulates myocardial injury by secreting paracrine factors. J Clin Invest. 2011 May; 121(5):1894-904. View abstract
  43. Concise review: Vascular stem cells and tumor angiogenesis. Stem Cells. 2011 Feb; 29(2):163-8. View abstract
  44. Type I collagen, fibrin and PuraMatrix matrices provide permissive environments for human endothelial and mesenchymal progenitor cells to form neovascular networks. J Tissue Eng Regen Med. 2011 Apr; 5(4):e74-86. View abstract
  45. Functional endothelial progenitor cells from cryopreserved umbilical cord blood. Cell Transplant. 2011; 20(4):515-22. View abstract
  46. Host myeloid cells are necessary for creating bioengineered human vascular networks in vivo. Tissue Eng Part A. 2010 Aug; 16(8):2457-66. View abstract
  47. Intravital molecular imaging of small-diameter tissue-engineered vascular grafts in mice: a feasibility study. Tissue Eng Part C Methods. 2010 Aug; 16(4):597-607. View abstract
  48. Bone marrow is a reservoir for proangiogenic myelomonocytic cells but not endothelial cells in spontaneous tumors. Blood. 2010 Oct 28; 116(17):3367-71. View abstract
  49. In vivo monitoring of function of autologous engineered pulmonary valve. J Thorac Cardiovasc Surg. 2010 Mar; 139(3):723-31. View abstract
  50. Methodology for optimal in vitro cell expansion in tissue engineering. Adv Biochem Eng Biotechnol. 2009; 112:209-29. View abstract
  51. Multipotential stem cells recapitulate human infantile hemangioma in immunodeficient mice. J Clin Invest. 2008 Jul; 118(7):2592-9. View abstract
  52. Engineering robust and functional vascular networks in vivo with human adult and cord blood-derived progenitor cells. Circ Res. 2008 Jul 18; 103(2):194-202. View abstract
  53. Chapter 13. An in vivo experimental model for postnatal vasculogenesis. Methods Enzymol. 2008; 445:303-29. View abstract
  54. In vivo vasculogenic potential of human blood-derived endothelial progenitor cells. Blood. 2007 Jun 01; 109(11):4761-8. View abstract
  55. Human pulmonary valve progenitor cells exhibit endothelial/mesenchymal plasticity in response to vascular endothelial growth factor-A and transforming growth factor-beta2. Circ Res. 2006 Oct 13; 99(8):861-9. View abstract
  56. Endothelial progenitor cells from infantile hemangioma and umbilical cord blood display unique cellular responses to endostatin. Blood. 2006 Aug 01; 108(3):915-21. View abstract
  57. Expansion of chondroprogenitor cells on macroporous microcarriers as an alternative to conventional monolayer systems. Biomaterials. 2006 May; 27(15):2970-9. View abstract
  58. Optimal in-vitro expansion of chondroprogenitor cells in monolayer culture. Biotechnol Bioeng. 2006 Feb 20; 93(3):519-33. View abstract