ABOUT THE RESEARCHER

OVERVIEW

My research focuses on the development of novel tissue engineering strategies for hollow organ repair and regeneration. Specifically, I investigate the utility of silk scaffolds as platforms for urogenital, gastrointestinal, and respiratory tissue reconstruction. By deciphering how variations in scaffold processing parameters can affect the in vivo performance of silk biomaterials, we seek to develop 3-D matrix designs with optimal structural, mechanical, and degradation profiles capable of restoring function in defects associated with traumatic injury and fibrotic diseases. In collaboration with our clinical partner, Dr. Carlos Estrada, MD, we employ both small and large animal models of tissue repair in the bladder, urethra, small intestine, esophagus, and trachea. In addition, my laboratory explores the mechanisms of constructive remodeling of tissue engineered constructs by delineating how host tissue microenvironments interact, populate, and integrate into scaffold environments in vivo. Our overall goal is the creation of clinically-useful scaffold configurations for hollow organ regeneration by engineering materials which fulfill structural and mechanical requirements of native tissues as well as present microenvironmental cues necessary for host tissue integration and defect consolidation. My comprehensive background in silk-based biomaterial fabrication methods coupled with my expertise in stem cell biology uniquely positions my lab as a pioneer in the creation of novel strategies for hollow organ regeneration.

Selected Publications

  1. Tu DD, Chung YG, Gil ES, Seth A, Franck D, Cristofaro V, Sullivan MP, Di Vizio D, Gomez P 3rd, Adam RM, Kaplan DL, Estrada CR Jr, Mauney JR. Bladder tissue regeneration using acellular bi-layer silk scaffolds in a large animal model of augmentation cystoplasty. Biomaterials. 2013 Nov;34(34):8681-9. PMID: 23953839.
  2. Chung YG, Tu D, Franck D, Gil ES, Algarrahi K, Adam RM, Kaplan DL, Estrada CR Jr, Mauney JR. Acellular bi-layer silk fibroin scaffolds support tissue regeneration in a rabbit model of onlay urethroplasty. PLoS One. 2014 Mar 14;9(3):e91592. PMID: 24632740.
  3. Chung YG, Algarrahi K, Franck D, Duong DD, Adam RM, Kaplan DL, Estrada CR, Mauney JR. The Use of Bi-layer Silk Fibroin Scaffolds and Small Intestinal Submucosa Matrices to Support Bladder Tissue Regeneration in a Rat Model of Spinal Cord Injury. 2014 Biomaterials. Aug;35(26):7452-9. PMID: 24917031.
  4. Algarrahi K, Franck D, Ghezzi C, Cristafaro V, Yang X, Sullivan MP, Chung YG, Affas S, Jennings R, Kaplan DL, Estrada CR, Mauney JR. Acellular Bi-Layer Silk Fibroin Scaffolds Support Functional Tissue Regeneration in a Rat Model of Onlay Esophagoplasty. Biomaterials. 2015 Jun;53:149-59.2015. PMID: 25890715

See a complete list of published work in my bibliography.

PUBLICATIONS

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  1. Molecular mechanisms of esophageal epithelial regeneration following repair of surgical defects with acellular silk fibroin grafts. Sci Rep. 2021 Mar 29; 11(1):7086. View abstract
  2. Evaluation of Bilayer Silk Fibroin Grafts for Tubular Esophagoplasty in a Porcine Defect Model. Tissue Eng Part A. 2021 01; 27(1-2):103-116. View abstract
  3. Augmentation Cystoplasty of Diseased Porcine Bladders with Bi-Layer Silk Fibroin Grafts. Tissue Eng Part A. 2019 06; 25(11-12):855-866. View abstract
  4. Evaluation of Acellular Bilayer Silk Fibroin Grafts for Onlay Tracheoplasty in a Rat Defect Model. Otolaryngol Head Neck Surg. 2019 02; 160(2):310-319. View abstract
  5. Repair of injured urethras with silk fibroin scaffolds in a rabbit model of onlay urethroplasty. J Surg Res. 2018 09; 229:192-199. View abstract
  6. Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair. Stem Cell Reports. 2017 12 12; 9(6):2005-2017. View abstract
  7. Bilayer silk fibroin grafts support functional oesophageal repair in a rodent model of caustic injury. J Tissue Eng Regen Med. 2018 02; 12(2):e1068-e1075. View abstract
  8. Bi-layer silk fibroin grafts support functional tissue regeneration in a porcine model of onlay esophagoplasty. J Tissue Eng Regen Med. 2018 02; 12(2):e894-e904. View abstract
  9. Silk Fibroin Scaffolds for Urologic Tissue Engineering. Curr Urol Rep. 2016 Feb; 17(2):16. View abstract
  10. Inosine Improves Neurogenic Detrusor Overactivity following Spinal Cord Injury. PLoS One. 2015; 10(11):e0141492. View abstract
  11. Acellular bi-layer silk fibroin scaffolds support functional tissue regeneration in a rat model of onlay esophagoplasty. Biomaterials. 2015 Jun; 53:149-59. View abstract
  12. In vitro evaluation of bi-layer silk fibroin scaffolds for gastrointestinal tissue engineering. J Tissue Eng. 2014; 5:2041731414556849. View abstract
  13. Dynamic reciprocity in cell-scaffold interactions. Adv Drug Deliv Rev. 2015 Mar; 82-83:77-85. View abstract
  14. The use of bi-layer silk fibroin scaffolds and small intestinal submucosa matrices to support bladder tissue regeneration in a rat model of spinal cord injury. Biomaterials. 2014 Aug; 35(26):7452-9. View abstract
  15. Acellular bi-layer silk fibroin scaffolds support tissue regeneration in a rabbit model of onlay urethroplasty. PLoS One. 2014; 9(3):e91592. View abstract
  16. Retinoid signaling in progenitors controls specification and regeneration of the urothelium. Dev Cell. 2013 Sep 16; 26(5):469-482. View abstract
  17. Bladder tissue regeneration using acellular bi-layer silk scaffolds in a large animal model of augmentation cystoplasty. Biomaterials. 2013 Nov; 34(34):8681-9. View abstract
  18. The performance of silk scaffolds in a rat model of augmentation cystoplasty. Biomaterials. 2013 Jul; 34(20):4758-65. View abstract
  19. Evaluation of silk biomaterials in combination with extracellular matrix coatings for bladder tissue engineering with primary and pluripotent cells. PLoS One. 2013; 8(2):e56237. View abstract
  20. Evaluation of biomaterials for bladder augmentation using cystometric analyses in various rodent models. J Vis Exp. 2012 Aug 09; (66). View abstract
  21. When urothelial differentiation pathways go wrong: implications for bladder cancer development and progression. Urol Oncol. 2013 Aug; 31(6):802-11. View abstract
  22. The effect of manipulation of silk scaffold fabrication parameters on matrix performance in a murine model of bladder augmentation. Biomaterials. 2011 Oct; 32(30):7562-70. View abstract
  23. An hTERT-immortalized human urothelial cell line that responds to anti-proliferative factor. In Vitro Cell Dev Biol Anim. 2011 Jan; 47(1):2-9. View abstract
  24. Evaluation of gel spun silk-based biomaterials in a murine model of bladder augmentation. Biomaterials. 2011 Jan; 32(3):808-18. View abstract
  25. Matrix remodeling as stem cell recruitment event: a novel in vitro model for homing of human bone marrow stromal cells to the site of injury shows crucial role of extracellular collagen matrix. Matrix Biol. 2010 Oct; 29(8):657-63. View abstract
  26. All-trans retinoic acid directs urothelial specification of murine embryonic stem cells via GATA4/6 signaling mechanisms. PLoS One. 2010 Jul 13; 5(7):e11513. View abstract
  27. Adult human bone marrow stromal cells regulate expression of their MMPs and TIMPs in differentiation type-specific manner. Matrix Biol. 2010 Jan; 29(1):3-8. View abstract
  28. Human bone marrow-derived stromal cells show highly efficient stress-resistant adipogenesis on denatured collagen IV matrix but not on its native counterpart: implications for obesity. Matrix Biol. 2010 Jan; 29(1):9-14. View abstract
  29. Progression of human bone marrow stromal cells into both osteogenic and adipogenic lineages is differentially regulated by structural conformation of collagen I matrix via distinct signaling pathways. Matrix Biol. 2009 Jun; 28(5):239-50. View abstract
  30. Collagen I matrix contributes to determination of adult human stem cell lineage via differential, structural conformation-specific elicitation of cellular stress response. Matrix Biol. 2009 Jun; 28(5):251-62. View abstract
  31. Denatured collagen modulates the phenotype of normal and wounded human skin equivalents. J Invest Dermatol. 2008 Jul; 128(7):1830-7. View abstract
  32. Tissue-engineered bone serves as a target for metastasis of human breast cancer in a mouse model. Cancer Res. 2007 Nov 01; 67(21):10304-8. View abstract
  33. Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds. Biomaterials. 2007 Dec; 28(35):5280-90. View abstract
  34. Studies of osteotropism on both sides of the breast cancer-bone interaction. Ann N Y Acad Sci. 2007 Nov; 1117:328-44. View abstract
  35. Matrix-mediated retention of in vitro osteogenic differentiation potential and in vivo bone-forming capacity by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion. J Biomed Mater Res A. 2006 Dec 01; 79(3):464-75. View abstract
  36. Matrix-mediated retention of adipogenic differentiation potential by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion. Biomaterials. 2005 Nov; 26(31):6167-75. View abstract
  37. In vitro and in vivo evaluation of differentially demineralized cancellous bone scaffolds combined with human bone marrow stromal cells for tissue engineering. Biomaterials. 2005 Jun; 26(16):3173-85. View abstract
  38. Role of adult mesenchymal stem cells in bone tissue engineering applications: current status and future prospects. Tissue Eng. 2005 May-Jun; 11(5-6):787-802. View abstract
  39. Matrix-mediated retention of osteogenic differentiation potential by human adult bone marrow stromal cells during ex vivo expansion. Biomaterials. 2004 Jul; 25(16):3233-43. View abstract
  40. Mechanical stimulation promotes osteogenic differentiation of human bone marrow stromal cells on 3-D partially demineralized bone scaffolds in vitro. Calcif Tissue Int. 2004 May; 74(5):458-68. View abstract
  41. Osteogenic differentiation of human bone marrow stromal cells on partially demineralized bone scaffolds in vitro. Tissue Eng. 2004 Jan-Feb; 10(1-2):81-92. View abstract