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Techniques | Overview

  • Phenotypic characterization of mouse models of disease

  • DTI and DSI MR of adult and embryonic mouse brains

  • Analysis of isolated embryonic neurons from hippocampus, cortex, spinal cord, oculomotor anlage

  • Growth assays of explant cultures of DRG, embryonic spinal cord, midbrain & hindbrain

  • Transfection, electroporation and siRNA treatment of neurons & other cells

  • Microscopy: confocal, deconvolution, EM

  • Morphometrics; kymography

  • DiO, DiI multispectrum anterograde & retrograde labeling of axonal tracks

  • Live cell imaging of kinesin motility & microtubule dynamics

  • Ultracentrifugal methods: cell fractionation, sucrose gradient separation of protein complexes

  • Microtubule biochemical assays

  • Immunoprecipitation, epitope-based pull downs & immunoblotting

  • Affinity chromatography protein purification

  • Mass spec proteomics for identification of protein partners

  • Native and SDS gel electrophoresis

  • Cell signaling assays

  • Real-time RT-PCR

  • Mutation analysis: DNA sequencing, WAVE analysis, genome-wide screens (GWS)