Leslie S. Kean, MD, PhD
Director, Stem Cell Transplant Center, Dana-Farber/Boston Children's Cancer and Blood Disorders Center
Robert A. Stranahan Professor of Pediatrics, Harvard Medical School
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Leslie S. Kean, MD, PhD
Director, Stem Cell Transplant Center, Dana-Farber/Boston Children's Cancer and Blood Disorders Center
Robert A. Stranahan Professor of Pediatrics, Harvard Medical School
Medical Services
Languages
English
Education
Medical School
Emory University
1999
Atlanta
GA
Residency
Emory University School of Medicine
2003
Atlanta
GA
Fellowship
Aflac Cancer Center and Blood Disorders Service
Emory University School of Medicine
2005
Atlanta
GA
Certifications
American Board of Pediatrics (Hematology-Oncology)
Publications
Impact of Post-Transplant Cyclophosphamide-Based GVHD Prophylaxis in Patients 70 years and Older: An Update from BMT CTN 1703. View Abstract
Accurate prediction of absolute prokaryotic abundance from DNA concentration. View Abstract
Graft-versus-host disease prophylaxis shapes T cell biology and immune reconstitution after hematopoietic cell transplant. View Abstract
Deficiency of T follicular helper cell Tet3 DNA demethylation inhibits pathogenic IgG2c class switching and chronic GVHD. View Abstract
Organ-specific microenvironments drive divergent T cell evolution in acute graft-versus-host disease. View Abstract
Improved Patient-Reported Outcomes With Post-Transplant Cyclophosphamide: A Quality-of-Life Evaluation and 2-Year Outcomes of BMT CTN 1703. View Abstract
Abatacept for acute graft-versus-host disease prophylaxis after unrelated donor hematopoietic cell transplantation. View Abstract
Human OX40L-CAR-Tregs target activated antigen-presenting cells and control T cell alloreactivity. View Abstract
Correction: Harmonizing definitions for hematopoietic recovery, graft rejection, graft failure, poor graft function, and donor chimerism in allogeneic hematopoietic cell transplantation: a report on behalf of the EBMT, ASTCT, CIBMTR, and APBMT. View Abstract
Systematic identification of minor histocompatibility antigens predicts outcomes of allogeneic hematopoietic cell transplantation. View Abstract
B-cell-directed CAR T-cell therapy activates CD8+ cytotoxic CARneg bystander T cells in patients and nonhuman primates. View Abstract
Alloengraftment without significant toxicity or GVHD in CD45 antibody-drug conjugate-conditioned Fanconi anemia mice. View Abstract
Major breakthroughs in hematopoietic stem cell transplantation and future challenges in clinical implementation. View Abstract
The Adverse Event Landscape of Stem Cell Transplant: Evidence for AGVHD Driving Early Transplant Associated Toxicities. View Abstract
Harmonizing definitions for hematopoietic recovery, graft rejection, graft failure, poor graft function, and donor chimerism in allogeneic hematopoietic cell transplantation: a report on behalf of the EBMT, ASTCT, CIBMTR, and APBMT. View Abstract
Overlap chronic GVHD is associated with adverse survival outcomes compared to classic chronic GVHD. View Abstract
Higher abatacept exposure after transplant decreases acute GVHD risk without increasing adverse events. View Abstract
Abatacept for the prevention of GVHD in patients receiving mismatched unrelated transplants: a real-world analysis. View Abstract
Real-world experience with low-dose IL-2 for children and young adults with refractory chronic graft-versus-host disease. View Abstract
Abatacept for the prevention of graft versus host disease in pediatric patients receiving 7/8 HLA-mismatched unrelated transplant for hematologic malignancies: a real-world analysis. View Abstract
Abatacept for graft versus host disease prophylaxis in patients 60 years and older receiving mismatched unrelated donor transplantation for hematologic malignancies. View Abstract
Alemtuzumab and CXCL9 levels predict likelihood of sustained engraftment after reduced-intensity conditioning HCT. View Abstract
Notch signaling drives intestinal graft-versus-host disease in mice and nonhuman primates. View Abstract
Post-Transplantation Cyclophosphamide-Based Graft-versus-Host Disease Prophylaxis. View Abstract
Real-world experience with low-dose IL-2 for children and young adults with refractory chronic graft-versus-host disease. View Abstract
Abatacept GVHD prophylaxis in unrelated hematopoietic cell transplantation for pediatric bone marrow failure. View Abstract
Pre-existing immunity does not impair the engraftment of CRISPR-Cas9-edited cells in rhesus macaques conditioned with busulfan or radiation. View Abstract
Engineering T cells to suppress acute GVHD and leukemia relapse after allogeneic hematopoietic stem cell transplantation. View Abstract
Enforced gut homing of murine regulatory T cells reduces early graft-versus-host disease severity. View Abstract
Case Report: The First Case Report of Visceral Leishmaniasis in Cambodia. View Abstract
Retinoic acid signaling acts as a rheostat to balance Treg function. View Abstract
BET-bromodomain and EZH2 inhibitor-treated chronic GVHD mice have blunted germinal centers with distinct transcriptomes. View Abstract
Abatacept for GVHD prophylaxis can reduce racial disparities by abrogating the impact of mismatching in unrelated donor stem cell transplantation. View Abstract
Identification and Tracking of Alloreactive T Cell Clones in Rhesus Macaques Through the RM-scTCR-Seq Platform. View Abstract
Myelopathy Because of CAR-T-Related Neurotoxicity Treated With Siltuximab. View Abstract
Blood and Marrow Transplant Clinical Trials Network State of the Science Symposium 2021: Looking Forward as the Network Celebrates its 20th Year. View Abstract
Standardizing Definitions of Hematopoietic Recovery, Graft Rejection, Graft Failure, Poor Graft Function, and Donor Chimerism in Allogeneic Hematopoietic Cell Transplantation: A Report on Behalf of the American Society for Transplantation and Cellular Therapy. View Abstract
Insights from integrating clinical and preclinical studies advance understanding of graft-versus-host disease. View Abstract
Blueprint for the discovery of biomarkers of toxicity and efficacy for CAR T cells and T-cell engagers. View Abstract
Multiply restimulated human thymic regulatory T cells express distinct signature regulatory T-cell transcription factors without evidence of exhaustion. View Abstract
Repurposing a novel anti-cancer RXR agonist to attenuate murine acute GVHD and maintain graft-versus-leukemia responses. View Abstract
Phase II Trial of Costimulation Blockade With Abatacept for Prevention of Acute GVHD. View Abstract
Spatiotemporal single-cell profiling reveals that invasive and tissue-resident memory donor CD8+ T cells drive gastrointestinal acute graft-versus-host disease. View Abstract
Measurement of leukocyte trafficking kinetics in macaques by serial intravascular staining. View Abstract
Creating an international network for science and clinical progress: democratizing dissemination of advances in global hematology. View Abstract
Current Concepts and Advances in Graft-Versus-Host Disease Immunology. View Abstract
Author Correction: Innovations present in the primate interneuron repertoire. View Abstract
End the pain: Start with antiracism. View Abstract
Robust expansion of HIV CAR T cells following antigen boosting in ART-suppressed nonhuman primates. View Abstract
Isolation of a Highly Purified HSC-enriched CD34+CD90+CD45RA- Cell Subset for Allogeneic Transplantation in the Nonhuman Primate Large-animal Model. View Abstract
Risk factors for transplant-associated thrombotic microangiopathy and mortality in a pediatric cohort. View Abstract
Single-Cell Analyses of Colon and Blood Reveal Distinct Immune Cell Signatures of Ulcerative Colitis and Crohn's Disease. View Abstract
IL-2 enhances ex vivo-expanded regulatory T-cell persistence after adoptive transfer. View Abstract
SARS-CoV-2 Receptor ACE2 Is an Interferon-Stimulated Gene in Human Airway Epithelial Cells and Is Detected in Specific Cell Subsets across Tissues. View Abstract
A phase I study of sirolimus in combination with metronomic therapy (CHOAnome) in children with recurrent or refractory solid and brain tumors. View Abstract
Busulfan Combined with Immunosuppression Allows Efficient Engraftment of Gene-Modified Cells in a Rhesus Macaque Model. View Abstract
Characterization of natural killer cells expressing markers associated with maturity and cytotoxicity in children and young adults with sickle cell disease. View Abstract
Evidence for persistence of the SHIV reservoir early after MHC haploidentical hematopoietic stem cell transplantation. View Abstract
CD28 blockade controls T cell activation to prevent graft-versus-host disease in primates. View Abstract
Reduced-intensity conditioning for hematopoietic cell transplant for HLH and primary immune deficiencies. View Abstract
Defining success with cellular therapeutics: the current landscape for clinical end point and toxicity analysis. View Abstract
Human CD19-Targeted Mouse T Cells Induce B Cell Aplasia and Toxicity in Human CD19 Transgenic Mice. View Abstract
Chimeric Antigen Receptor T Cell-Mediated Neurotoxicity in Nonhuman Primates. View Abstract
Reprint of: Building a Safer and Faster CAR: Seatbelts, Airbags, and CRISPR. View Abstract
The Microbiome and Hematopoietic Cell Transplantation: Past, Present, and Future. View Abstract
Building a Safer and Faster CAR: Seatbelts, Airbags, and CRISPR. View Abstract
Combined OX40L and mTOR blockade controls effector T cell activation while preserving Treg reconstitution after transplant. View Abstract
In Vitro Induction of Human Regulatory T Cells Using Conditions of Low Tryptophan Plus Kynurenines. View Abstract
Advances in targeting co-inhibitory and co-stimulatory pathways in transplantation settings: the Yin to the Yang of cancer immunotherapy. View Abstract
A Canine Model of Chronic Graft-versus-Host Disease. View Abstract
Preclinical Testing of Antihuman CD28 Fab' Antibody in a Novel Nonhuman Primate Small Animal Rodent Model of Xenogenic Graft-Versus-Host Disease. View Abstract
Systems analysis uncovers inflammatory Th/Tc17-driven modules during acute GVHD in monkey and human T cells. View Abstract
The Knife's Edge of Tolerance: Inducing Stable Multilineage Mixed Chimerism but With a Significant Risk of CMV Reactivation and Disease in Rhesus Macaques. View Abstract
Programmed death ligand-1 expression on donor T cells drives graft-versus-host disease lethality. View Abstract
Transcriptome analysis of GVHD reveals aurora kinase A as a targetable pathway for disease prevention. View Abstract
Immunophenotypic parameters and RBC alloimmunization in children with sickle cell disease on chronic transfusion. View Abstract
Use of Alefacept for Preconditioning in Multiply Transfused Pediatric Patients with Nonmalignant Diseases. View Abstract
Red blood cell transfusions are associated with HLA class I but not H-Y alloantibodies in children with sickle cell disease. View Abstract
CMV reactivation drives posttransplant T-cell reconstitution and results in defects in the underlying TCRß repertoire. View Abstract
Immune parameter analysis of children with sickle cell disease on hydroxycarbamide or chronic transfusion therapy. View Abstract
Superiority of rapamycin over tacrolimus in preserving nonhuman primate Treg half-life and phenotype after adoptive transfer. View Abstract
Persistence of virus reservoirs in ART-treated SHIV-infected rhesus macaques after autologous hematopoietic stem cell transplant. View Abstract
Renal transplantation using belatacept without maintenance steroids or calcineurin inhibitors. View Abstract
CD8-predominant T-cell CNS infiltration accompanies GVHD in primates and is improved with immunoprophylaxis. View Abstract
In vivo T cell costimulation blockade with abatacept for acute graft-versus-host disease prevention: a first-in-disease trial. View Abstract
Heterologous immunity triggered by a single, latent virus in Mus musculus: combined costimulation- and adhesion- blockade decrease rejection. View Abstract
Pluripotent stem cells: immune to the immune system? View Abstract
Cooking up tolerance: has a new recipe been created? View Abstract
Evidence for kidney rejection after combined bone marrow and renal transplantation despite ongoing whole-blood chimerism in rhesus macaques. View Abstract
Regulatory T cells exhibit decreased proliferation but enhanced suppression after pulsing with sirolimus. View Abstract
Nonhuman primate transplant models finally evolve: detailed immunogenetic analysis creates new models and strengthens the old. View Abstract
Significant mobilization of both conventional and regulatory T cells with AMD3100. View Abstract
CD40 blockade combines with CTLA4Ig and sirolimus to produce mixed chimerism in an MHC-defined rhesus macaque transplant model. View Abstract
Alloimmunization to transfused HOD red blood cells is not increased in mice with sickle cell disease. View Abstract
An MHC-defined primate model reveals significant rejection of bone marrow after mixed chimerism induction despite full MHC matching. View Abstract
GVHD after haploidentical transplantation: a novel, MHC-defined rhesus macaque model identifies CD28- CD8+ T cells as a reservoir of breakthrough T-cell proliferation during costimulation blockade and sirolimus-based immunosuppression. View Abstract
Expanded nonhuman primate tregs exhibit a unique gene expression signature and potently downregulate alloimmune responses. View Abstract
Expansion of effector memory TCR Vbeta4+ CD8+ T cells is associated with latent infection-mediated resistance to transplantation tolerance. View Abstract
NK cells rapidly reject allogeneic bone marrow in the spleen through a perforin- and Ly49D-dependent, but NKG2D-independent mechanism. View Abstract
Infusion of stably immature monocyte-derived dendritic cells plus CTLA4Ig modulates alloimmune reactivity in rhesus macaques. View Abstract
Induction of chimerism in rhesus macaques through stem cell transplant and costimulation blockade-based immunosuppression. View Abstract
Transplant tolerance in non-human primates: progress, current challenges and unmet needs. View Abstract
NK cells mediate costimulation blockade-resistant rejection of allogeneic stem cells during nonmyeloablative transplantation. View Abstract
Chimerism and cure: hematologic and pathologic correction of murine sickle cell disease. View Abstract
Comparison of mechanisms of anemia in mice with sickle cell disease and beta-thalassemia: peripheral destruction, ineffective erythropoiesis, and phospholipid scramblase-mediated phosphatidylserine exposure. View Abstract
A cure for murine sickle cell disease through stable mixed chimerism and tolerance induction after nonmyeloablative conditioning and major histocompatibility complex-mismatched bone marrow transplantation. View Abstract
Costimulation blockade, busulfan, and bone marrow promote titratable macrochimerism, induce transplantation tolerance, and correct genetic hemoglobinopathies with minimal myelosuppression. View Abstract