LIke ThisLIke ThisLIke This

Natasha Barteneva, MD, PhD

Man no face
Program in Cellular and Molecular Medicine (PCMM)
Hospital Title:
Director of Flow and Imaging Cytometry Resource
Academic Title:
Lecturer on Pediatrics, Harvard Medical School
Contact Via Email
Send an email to SendYour Name*Your Email Address*Subject*Comments*
Dr Barteneva’s research interests are focused on applying biophotonics and novel optical technologies to understanding of biological and biomedical problems. Imaging flow cytometry. Spectral flow cytometry. Eclectic interdisciplinary mix of topics in microbiology and cellular biology. Extracellular vesicles.


Publications powered by Harvard Catalyst Profiles
  1. Editors: Barteneva NS, Vorobjev IA. Methods in Molecular Biology. Cellular Heterogeneity: Methods and Protocols. 2018; 363 pp.
  2. Doan M, Vorobjev I, Rees P, Filby A, Wolkenhauer O, Goldfeld AE, Lieberman J, Barteneva N, Carpenter AE, Hennig H. Diagnostic Potential of Imaging Flow Cytometry. Trends Biotechnol. 2018 Jan 30.
  3. Veremeyko T, Yung AWY, Dukhinova M, Kuznetsova IS, Pomytkin I, Lyundup A, Strekalova T, Barteneva NS, Ponomarev ED. Cyclic AMP Pathway Suppress Autoimmune Neuroinflammation by Inhibiting Functions of Encephalitogenic CD4 T Cells and Enhancing M2 Macrophage Polarization at the Site of Inflammation. Front Immunol. 2018; 9:50.
  4. Trifonova RT, Barteneva NS. Quantitation of IRF3 Nuclear Translocation in Heterogeneous Cellular Populations from Cervical Tissue Using Imaging Flow Cytometry. Methods Mol Biol. 2018; 1745:125-153.
  5. Dashkova V, Clapper J, Vorobjev IA, Barteneva NS. Spectral and Imaging Flow Cytometry in Phytoplankton Research. Methods Mol Biol. 2018; 1745:83-95.
  6. Barteneva NS, Vorobjev IA. Heterogeneity of Metazoan Cells and Beyond: To Integrative Analysis of Cellular Populations at Single-Cell Level. Methods Mol Biol. 2018; 1745:3-23.
  7. Gladkikh AA, Potashnikova DM, Tatarskiy V, Yastrebova M, Khamidullina A, Barteneva N, Vorobjev I. Comparison of the mRNA expression profile of B-cell receptor components in normal CD5-high B-lymphocytes and chronic lymphocytic leukemia: a key role of ZAP70. Cancer Med. 2017 Dec; 6(12):2984-2997.
  8. Barteneva NS, Baiken Y, Fasler-Kan E, Alibek K, Wang S, Maltsev N, Ponomarev ED, Sautbayeva Z, Kauanova S, Moore A, Beglinger C, Vorobjev IA. Extracellular vesicles in gastrointestinal cancer in conjunction with microbiota: On the border of Kingdoms. Biochim Biophys Acta. 2017 12; 1868(2):372-393.
  9. Segev E, Wyche TP, Kim KH, Petersen J, Ellebrandt C, Vlamakis H, Barteneva N, Paulson JN, Chai L, Clardy J, Kolter R. Dynamic metabolic exchange governs a marine algal-bacterial interaction. Elife. 2016 11 18; 5.
  10. Haridas V, Ranjbar S, Vorobjev IA, Goldfeld AE, Barteneva NS. Imaging flow cytometry analysis of intracellular pathogens. Methods. 2017 Jan 01; 112:91-104.
  11. Show all
  12. Vorobjev IA, Barteneva NS. Multi-parametric imaging of cell heterogeneity in apoptosis analysis. Methods. 2017 Jan 01; 112:105-123.
  13. Dashkova V, Malashenkov D, Poulton N, Vorobjev I, Barteneva NS. Imaging flow cytometry for phytoplankton analysis. Methods. 2017 Jan 01; 112:188-200.
  14. Wheeler LA, Trifonova RT, Vrbanac V, Barteneva NS, Liu X, Bollman B, Onofrey L, Mulik S, Ranjbar S, Luster AD, Tager AM, Lieberman J. TREX1 Knockdown Induces an Interferon Response to HIV that Delays Viral Infection in Humanized Mice. Cell Rep. 2016 05 24; 15(8):1715-27.
  15. Dashkova V, Segev E, Malashenkov D, Kolter R, Vorobjev I, Barteneva NS. Microalgal cytometric analysis in the presence of endogenous autofluorescent pigments. Algal Research. 2016.
  16. Editors: Barteneva NS, Vorobjev IA. Methods in Molecular Biology Series. Imaging Flow Cytometry. 2016; 295 pp.
  17. Vorobjev IA, Barteneva NS. Temporal Heterogeneity in Apoptosis Determined by Imaging Flow Cytometry. Methods Mol Biol. 2016; 1389:221-33.
  18. Fasler-Kan E, Baiken Y, Vorobjev IA, Barteneva NS. Analysis of Nucleocytoplasmic Protein Shuttling by Imaging Flow Cytometry. Methods Mol Biol. 2016; 1389:127-37.
  19. Vorobjev IA, Barteneva NS. Quantitative Functional Morphology by Imaging Flow Cytometry. Methods Mol Biol. 2016; 1389:3-11.
  20. Yoo B, Kavishwar A, Ross A, Wang P, Tabassum DP, Polyak K, Barteneva N, Petkova V, Pantazopoulos P, Tena A, Moore A, Medarova Z. Combining miR-10b-Targeted Nanotherapy with Low-Dose Doxorubicin Elicits Durable Regressions of Metastatic Breast Cancer. Cancer Res. 2015 Oct 15; 75(20):4407-15.
  21. Egan ES, Jiang RH, Moechtar MA, Barteneva NS, Weekes MP, Nobre LV, Gygi SP, Paulo JA, Frantzreb C, Tani Y, Takahashi J, Watanabe S, Goldberg J, Paul AS, Brugnara C, Root DE, Wiegand RC, Doench JG, Duraisingh MT. Malaria. A forward genetic screen identifies erythrocyte CD55 as essential for Plasmodium falciparum invasion. Science. 2015 May 08; 348(6235):711-4.
  22. Vorobjev I, Barteneva NS. Temporal Heterogeneity Metrics in Apoptosis Induced by Anticancer Drugs. J Histochem Cytochem. 2015 07; 63(7):494-510.
  23. Pelle KG, Oh K, Buchholz K, Narasimhan V, Joice R, Milner DA, Brancucci NM, Ma S, Voss TS, Ketman K, Seydel KB, Taylor TE, Barteneva NS, Huttenhower C, Marti M. Transcriptional profiling defines dynamics of parasite tissue sequestration during malaria infection. Genome Med. 2015; 7(1):19.
  24. Padmanabhan PK, Polidoro RB, Barteneva NS, Gazzinelli RT, Burleigh BA. Transient transfection and expression of foreign and endogenous genes in the intracellular stages of Trypanosoma cruzi. Mol Biochem Parasitol. 2014 Dec; 198(2):100-3.
  25. Gaudin R, Barteneva NS. Sorting of small infectious virus particles by flow virometry reveals distinct infectivity profiles. Nat Commun. 2015 Feb 02; 6:6022.
  26. Gaiha GD, McKim KJ, Woods M, Pertel T, Rohrbach J, Barteneva N, Chin CR, Liu D, Soghoian DZ, Cesa K, Wilton S, Waring MT, Chicoine A, Doering T, Wherry EJ, Kaufmann DE, Lichterfeld M, Brass AL, Walker BD. Dysfunctional HIV-specific CD8+ T cell proliferation is associated with increased caspase-8 activity and mediated by necroptosis. Immunity. 2014 Dec 18; 41(6):1001-12.
  27. Yoo B, Kavishwar A, Ghosh SK, Barteneva N, Yigit MV, Moore A, Medarova Z. Detection of miRNA expression in intact cells using activatable sensor oligonucleotides. Chem Biol. 2014 Feb 20; 21(2):199-204.
  28. Barteneva NS, Ponomarev ED, Tsytsykova A, Armant M, Vorobjev IA. Mitochondrial staining allows robust elimination of apoptotic and damaged cells during cell sorting. J Histochem Cytochem. 2014 Apr; 62(4):265-75.
  29. Fasler-Kan E, Barteneva NS, Ketterer S, Wunderlich K, Reschner A, Nurzhanova A, Flammer J, Huwyler J, Meyer P. Human cytokines activate JAK-STAT signaling pathway in porcine ocular tissue. Xenotransplantation. 2013 Nov-Dec; 20(6):469-80.
  30. Ghosh SK, Yigit MV, Uchida M, Ross AW, Barteneva N, Moore A, Medarova Z. Sequence-dependent combination therapy with doxorubicin and a survivin-specific small interfering RNA nanodrug demonstrates efficacy in models of adenocarcinoma. Int J Cancer. 2014 Apr 01; 134(7):1758-66.
  31. Barteneva NS, Maltsev N, Vorobjev IA. Microvesicles and intercellular communication in the context of parasitism. Front Cell Infect Microbiol. 2013; 3:49.
  32. Mantel PY, Hoang AN, Goldowitz I, Potashnikova D, Hamza B, Vorobjev I, Ghiran I, Toner M, Irimia D, Ivanov AR, Barteneva N, Marti M. Malaria-infected erythrocyte-derived microvesicles mediate cellular communication within the parasite population and with the host immune system. Cell Host Microbe. 2013 May 15; 13(5):521-34.
  33. Barteneva NS, Fasler-Kan E, Bernimoulin M, Stern JN, Ponomarev ED, Duckett L, Vorobjev IA. Circulating microparticles: square the circle. BMC Cell Biol. 2013 Apr 22; 14:23.
  34. Sotnikov I, Veremeyko T, Starossom SC, Barteneva N, Weiner HL, Ponomarev ED. Platelets recognize brain-specific glycolipid structures, respond to neurovascular damage and promote neuroinflammation. PLoS One. 2013; 8(3):e58979.
  35. Barteneva NS, Ketman K, Fasler-Kan E, Potashnikova D, Vorobjev IA. Cell sorting in cancer research--diminishing degree of cell heterogeneity. Biochim Biophys Acta. 2013 Aug; 1836(1):105-22.
  36. Vorobjev IA, Buchholz K, Prabhat P, Ketman K, Egan ES, Marti M, Duraisingh MT, Barteneva NS. Optimization of flow cytometric detection and cell sorting of transgenic Plasmodium parasites using interchangeable optical filters. Malar J. 2012 Sep 05; 11:312.
  37. Barteneva NS, Fasler-Kan E, Vorobjev IA. Imaging flow cytometry: coping with heterogeneity in biological systems. J Histochem Cytochem. 2012 Oct; 60(10):723-33.
  38. Ponomarev ED, Veremeyko T, Barteneva NS. Visualization and quantitation of the expression of microRNAs and their target genes in neuroblastoma single cells using imaging cytometry. BMC Res Notes. 2011 Nov 28; 4:517.
  39. Kovalenko EI, Ranjbar S, Jasenosky LD, Goldfeld AE, Vorobjev IA, Barteneva NS. The use of HaloTag-based technology in flow and laser scanning cytometry analysis of live and fixed cells. BMC Res Notes. 2011 Sep 09; 4:340.
  40. Vorob'ev IA, Rafalovskaia-Orlovskaia EP, Gladkikh AA, Potashnikova DM, Barteneva NS. Tzytologia (Moscow). Applications of fluorescent semiconductor nanocrystals in microscopy and cytometry. 2011; 53(5):392-403.
  41. Vorob'ev IA, Rafalovskaia-Orlovskaia EP, Gladkikh AA, Potashnikova DM, Barteneva NS. [Applications of fluorescent semiconductor nanocrystals in microscopy and cytometry]. Tsitologiia. 2011; 53(5):392-403.
  42. Ponomarev ED, Veremeyko T, Barteneva N, Krichevsky AM, Weiner HL. MicroRNA-124 promotes microglia quiescence and suppresses EAE by deactivating macrophages via the C/EBP-a-PU.1 pathway. Nat Med. 2011 Jan; 17(1):64-70.
  43. Fasler-Kan E, Suenderhauf C, Barteneva N, Poller B, Gygax D, Huwyler J. Cytokine signaling in the human brain capillary endothelial cell line hCMEC/D3. Brain Res. 2010 Oct 01; 1354:15-22.
  44. Xu C, Lo A, Yammanuru A, Tallarico AS, Brady K, Murakami A, Barteneva N, Zhu Q, Marasco WA. Unique biological properties of catalytic domain directed human anti-CAIX antibodies discovered through phage-display technology. PLoS One. 2010 Mar 10; 5(3):e9625.
  45. Barrington RA, Schneider TJ, Pitcher LA, Mempel TR, Ma M, Barteneva NS, Carroll MC. Uncoupling CD21 and CD19 of the B-cell coreceptor. Proc Natl Acad Sci U S A. 2009 Aug 25; 106(34):14490-5.
  46. Ahmed F, Friend S, George TC, Barteneva N, Lieberman J. Numbers matter: quantitative and dynamic analysis of the formation of an immunological synapse using imaging flow cytometry. J Immunol Methods. 2009 Aug 15; 347(1-2):79-86.
  47. Bernimoulin M, Waters EK, Foy M, Steele BM, Sullivan M, Falet H, Walsh MT, Barteneva N, Geng JG, Hartwig JH, Maguire PB, Wagner DD. Differential stimulation of monocytic cells results in distinct populations of microparticles. J Thromb Haemost. 2009 Jun; 7(6):1019-28.
  48. Yew JY, Wang Y, Barteneva N, Dikler S, Kutz-Naber KK, Li L, Kravitz EA. Analysis of neuropeptide expression and localization in adult drosophila melanogaster central nervous system by affinity cell-capture mass spectrometry. J Proteome Res. 2009 Mar; 8(3):1271-84.
  49. Medarova Z, Kumar M, Ng SW, Yang J, Barteneva N, Evgenov NV, Petkova V, Moore A. Multifunctional magnetic nanocarriers for image-tagged SiRNA delivery to intact pancreatic islets. Transplantation. 2008 Nov 15; 86(9):1170-7.
  50. Muskavitch MA, Barteneva N, Gubbels MJ. Chemogenomics and parasitology: small molecules and cell-based assays to study infectious processes. Comb Chem High Throughput Screen. 2008 Sep; 11(8):624-46.
  51. Stern JN, Keskin DB, Barteneva N, Zuniga J, Yunis EJ, Ahmed AR. Possible role of natural killer cells in pemphigus vulgaris - preliminary observations. Clin Exp Immunol. 2008 Jun; 152(3):472-81.
  52. Maresz K, Ponomarev ED, Barteneva N, Tan Y, Mann MK, Dittel BN. IL-13 induces the expression of the alternative activation marker Ym1 in a subset of testicular macrophages. J Reprod Immunol. 2008 Jul; 78(2):140-8.
  53. Liu H, Schmidt-Supprian M, Shi Y, Hobeika E, Barteneva N, Jumaa H, Pelanda R, Reth M, Skok J, Rajewsky K, Shi Y. Yin Yang 1 is a critical regulator of B-cell development. Genes Dev. 2007 May 15; 21(10):1179-89.
  54. Kearns-Jonker M, Barteneva N, Mencel R, Hussain N, Shulkin I, Xu A, Yew M, Cramer DV. Use of molecular modeling and site-directed mutagenesis to define the structural basis for the immune response to carbohydrate xenoantigens. BMC Immunol. 2007 Mar 12; 8:3.
  55. Payne KJ, Huang G, Sahakian E, Zhu JY, Barteneva NS, Barsky LW, Payne MA, Crooks GM. Ikaros isoform x is selectively expressed in myeloid differentiation. J Immunol. 2003 Mar 15; 170(6):3091-8.
  56. Wu GD, Jin YS, Salazar R, Dai WD, Barteneva N, Barr ML, Barsky LW, Starnes VA, Cramer DV. Vascular endothelial cell apoptosis induced by anti-donor non-MHC antibodies: a possible injury pathway contributing to chronic allograft rejection. J Heart Lung Transplant. 2002 Nov; 21(11):1174-87.
  57. Khatsenko OG, Barteneva NS, de la Maza LM, Kikkawa Y. Role of nitric oxide in the inhibition of cytochrome P450 in the liver of mice infected with Chlamydia trachomatis. Biochem Pharmacol. 1998 Jun 01; 55(11):1835-42.
  58. Barteneva N, Theodor I, Peterson EM, de la Maza LM. Role of neutrophils in controlling early stages of a Chlamydia trachomatis infection. Infect Immun. 1996 Nov; 64(11):4830-3.
  59. Gorelov VN, Dumon K, Barteneva NS, Röher HD, Goretzki PE. A modified PCR-based method for rapid non-radioactive detection of clinically important pathogens. Microbiol Immunol. 1996; 40(9):611-6.
  60. Gorelov VN, Dumon K, Barteneva NS, Palm D, Röher HD, Goretzki PE. Overexpression of Gs alpha subunit in thyroid tumors bearing a mutated Gs alpha gene. J Cancer Res Clin Oncol. 1995; 121(4):219-24.
  61. Barteneva NS, Evstafieva AG, Gorelov VN, Wenzel BE. Identification and sequencing of a plasmid (pYV96)-encoded gene product of Yersinia enterocolitica recognized by antibodies in sera of patients with autoimmune thyroid disease. Ann N Y Acad Sci. 1994 Aug 15; 730:345-7.
  62. Volkov LV, Barteneva NS, Pronin AV, Markov AP, Fil'kova SL, Sanin AV, Smirnov GB. [The role of a plasmid with a molecular weight of 82 MD in the modulation of the action of Yersinia pseudotuberculosis on the proliferative activity of murine lymphoid cells]. Mol Gen Mikrobiol Virusol. 1991 Dec; (12):29-32.
  63. Smirnov GB, Shubin FN, Ginzburg AL, Shovadaeva G, Shaginyan IA, Ilyna TS, Markov AP, Pokrovskaya MS, Sever IS, Barteneva NS, et al. Molecular biology of Yersinia pseudotuberculosis large plasmid pVM82. Contrib Microbiol Immunol. 1991; 12:210-2.
  64. Sanin AV, Barteneva NS, Pronin AV. On the possible role of I-J+ suppressor cells in murine hematopoiesis. Ann N Y Acad Sci. 1991; 628:382-95.
  65. Khusainov RM, Ignatenko MA, Gritsenko LI, Frolova IS, Babaiants AA, Kuznetsov VP, Amchenkova AM, Narovlianskii AN, Pokidysheva LN, Barteneva NS, et al. [A new inducer of immune interferon in human leukocytes--the organogermanium compound MOP-11]. Vopr Virusol. 1991 Jan-Feb; 36(1):63-4.
LIke ThisLIke ThisLIke This