Research

LIke ThisLIke ThisLIke This

Joyce  Bischoff, PhD

Joyce Bischoff
Lab:
Bischoff Laboratory
Program:
Vascular Biology Program
Hospital Title:
Research Associate Department of Surgery
Academic Title:
Professor, Harvard Medical School

Research Overview

My lab studies the role of endothelial cells and pericytes in various settings of vascular disease and in the normal repair of the vasculature. Here I describe the four areas we are pursuing.

Infantile hemangioma is a vascular tumor that can grow rapidly, causing organ damage, disfigurement and morbidity. We’ve focused on identifying cellular mechanisms that drive this uncontrolled vascular growth. We identified a multi-potent stem cell that can recapitulate hemangioma in immune-deficient mice. We are also studying pericytes from hemangioma and the glucose transporter-1 positive endothelial cells, which are a hallmark of hemangioma. Our goal is to use cellular and animal models to identify new drugs that will work safely and quickly to prevent hemangiomas from growing to an endangering size.

Vascular malformations are distinct from ascular tumors such as hemangioma but are also little understood and medical therapies are needed. We have recently begun new projects on the cellular and molecular basis of capillary malformations, lymphatic malformation and venous malformations.

Endothelial progenitor cells (EPCs), also called endothelial colony forming cells (ECFCs) are rare cells found in the blood. They can be isolated based on their high proliferative capacity in vitro. We demonstrated the usefulness of EPC/ECFC for endothelializing small diameter vascular grafts in a sheep model and for building networks of human blood vessels rapidly in vivo using athymic nude mice. We also showed that ECFC were able to assemble into perfused vessels when injected into ischemic rat myocardium, and that the presence of these cells was beneficial to recovery of heart function over time.

Heart valve endothelial cells - The endothelial cells lining heart valves exhibit unique plasticity compared endothelial cells of large vessels (veins and arteries) or microvessels (capillaries). The valve endothelial cells can undergo endothelial to mesenchymal transition (EndMT) in response to TGFβ, and this process is highly regulated. We study the mitral valve endothelium as part of a multi-disciplinary team focused on how the mitral valve adapts after myocardial infarction.

About Joyce Bischoff

Joyce Bischoff received an A.B. in chemistry from Duke University and a Ph.D. in biochemistry from the Washington University School of Medicine. She was a post-doctoral fellow at the Whitehead Institute for Biomedical Research. She joined the Surgical Research Laboratories, now called the Vascular Biology Program, at Children's Hospital in 1990. She was elected to serve as President of the North American Vascular Biology Organization (NAVBO) from July 2015-June 2016.

Publications

Publications powered by Harvard Catalyst Profiles
  1. Bischoff J, Casanovas G, Wylie-Sears J, Kim DH, Bartko P, Guerrero J, Dal-Bianco J, Beaudoin J, Garcia M, Sullivan S, Seybolt M, Morris B, Keegan J, Irvin W, Aikawa E, Levine R. CD45 Expression in Mitral Valve Endothelial Cells After Myocardial Infarction. Circ Res. 2016 Oct 6.
  2. Ye X, Abou-Rayyah Y, Bischoff J, Ritchie A, Sebire NJ, Watts P, Churchill AJ, Bates DO. Altered ratios of pro- and anti-angiogenic VEGF-A variants and pericyte expression of DLL4 disrupt vascular maturation in infantile haemangioma. J Pathol. 2016 Jun; 239(2):139-51.
  3. Dal-Bianco JP, Bartko PE, Beaudoin J, Aikawa E, Bischoff J, Levine RA. 3D Ultrasound: seeing is understanding-from imaging to pathophysiology to developing therapies in secondary MR. Eur Heart J Cardiovasc Imaging. 2016 May; 17(5):510-1.
  4. Couto JA, Huang L, Vivero MP, Kamitaki N, Maclellan RA, Mulliken JB, Bischoff J, Warman ML, Greene AK. Endothelial Cells from Capillary Malformations Are Enriched for Somatic GNAQ Mutations. Plast Reconstr Surg. 2016 Jan; 137(1):77e-82e.
  5. Yu CW, Liang X, Lipsky S, Karaaslan C, Kozakewich H, Hotamisligil GS, Bischoff J, Cataltepe S. Dual role of fatty acid-binding protein 5 on endothelial cell fate: a potential link between lipid metabolism and angiogenic responses. Angiogenesis. 2016 Jan; 19(1):95-106.
  6. Levine RA, Hagége AA, Judge DP, Padala M, Dal-Bianco JP, Aikawa E, Beaudoin J, Bischoff J, Bouatia-Naji N, Bruneval P, Butcher JT, Carpentier A, Chaput M, Chester AH, Clusel C, Delling FN, Dietz HC, Dina C, Durst R, Fernandez-Friera L, Handschumacher MD, Jensen MO, Jeunemaitre XP, Le Marec H, Le Tourneau T, Markwald RR, Mérot J, Messas E, Milan DP, Neri T, Norris RA, Peal D, Perrocheau M, Probst V, Pucéat M, Rosenthal N, Solis J, Schott JJ, Schwammenthal E, Slaugenhaupt SA, Song JK, Yacoub MH. Mitral valve disease--morphology and mechanisms. Nat Rev Cardiol. 2015 Dec; 12(12):689-710.
  7. Hjortnaes J, Shapero K, Goettsch C, Hutcheson JD, Keegan J, Kluin J, Mayer JE, Bischoff J, Aikawa E. Valvular interstitial cells suppress calcification of valvular endothelial cells. Atherosclerosis. 2015 Sep; 242(1):251-60.
  8. Hofmann NA, Yang J, Trauger SA, Nakayama H, Huang L, Strunk D, Moses MA, Klagsbrun M, Bischoff J, Graier WF. The GPR 55 agonist, L-a-lysophosphatidylinositol, mediates ovarian carcinoma cell-induced angiogenesis. Br J Pharmacol. 2015 Aug; 172(16):4107-18.
  9. Smadja DM, Levy M, Huang L, Rossi E, Blandinières A, Israel-Biet D, Gaussem P, Bischoff J. Treprostinil indirectly regulates endothelial colony forming cell angiogenic properties by increasing VEGF-A produced by mesenchymal stem cells. Thromb Haemost. 2015 Oct; 114(4):735-47.
  10. Shapero K, Wylie-Sears J, Levine RA, Mayer JE, Bischoff J. Reciprocal interactions between mitral valve endothelial and interstitial cells reduce endothelial-to-mesenchymal transition and myofibroblastic activation. J Mol Cell Cardiol. 2015 Mar; 80:175-85.
  11. Show all
  12. Huang L, Nakayama H, Klagsbrun M, Mulliken JB, Bischoff J. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells. Stem Cells. 2015 Jan; 33(1):133-45.
  13. Boscolo E, Coma S, Luks VL, Greene AK, Klagsbrun M, Warman ML, Bischoff J. AKT hyper-phosphorylation associated with PI3K mutations in lymphatic endothelial cells from a patient with lymphatic malformation. Angiogenesis. 2015 Apr; 18(2):151-62.
  14. Gelfand MV, Hagan N, Tata A, Oh WJ, Lacoste B, Kang KT, Kopycinska J, Bischoff J, Wang JH, Gu C. Neuropilin-1 functions as a VEGFR2 co-receptor to guide developmental angiogenesis independent of ligand binding. Elife. 2014 Sep 22; 3:e03720.
  15. Lee D, Boscolo E, Durham JT, Mulliken JB, Herman IM, Bischoff J. Propranolol targets the contractility of infantile haemangioma-derived pericytes. Br J Dermatol. 2014 Nov; 171(5):1129-37.
  16. Smadja DM, Dorfmüller P, Guerin CL, Bieche I, Badoual C, Boscolo E, Kambouchner M, Cazes A, Mercier O, Humbert M, Gaussem P, Bischoff J, Israël-Biet D. Cooperation between human fibrocytes and endothelial colony-forming cells increases angiogenesis via the CXCR4 pathway. Thromb Haemost. 2014 Nov; 112(5):1002-13.
  17. Wylie-Sears J, Levine RA, Bischoff J. Losartan inhibits endothelial-to-mesenchymal transformation in mitral valve endothelial cells by blocking transforming growth factor-ß-induced phosphorylation of ERK. Biochem Biophys Res Commun. 2014 Apr 18; 446(4):870-5.
  18. Smadja DM, Guerin CL, Boscolo E, Bieche I, Mulliken JB, Bischoff J. a6-Integrin is required for the adhesion and vasculogenic potential of hemangioma stem cells. Stem Cells. 2014 Mar; 32(3):684-93.
  19. Allen P, Kang KT, Bischoff J. Rapid onset of perfused blood vessels after implantation of ECFCs and MPCs in collagen, PuraMatrix and fibrin provisional matrices. J Tissue Eng Regen Med. 2015 May; 9(5):632-6.
  20. Kolpa HJ, Peal DS, Lynch SN, Giokas AC, Ghatak S, Misra S, Norris RA, Macrae CA, Markwald RR, Ellinor P, Bischoff J, Milan DJ. miR-21 represses Pdcd4 during cardiac valvulogenesis. Development. 2013 May; 140(10):2172-80.
  21. Boscolo E, Mulliken JB, Bischoff J. Pericytes from infantile hemangioma display proangiogenic properties and dysregulated angiopoietin-1. Arterioscler Thromb Vasc Biol. 2013 Mar; 33(3):501-9.
  22. Smadja DM, Mulliken JB, Bischoff J. E-selectin mediates stem cell adhesion and formation of blood vessels in a murine model of infantile hemangioma. Am J Pathol. 2012 Dec; 181(6):2239-47.
  23. Camci-Unal G, Nichol JW, Bae H, Tekin H, Bischoff J, Khademhosseini A. Hydrogel surfaces to promote attachment and spreading of endothelial progenitor cells. J Tissue Eng Regen Med. 2013 May; 7(5):337-47.
  24. Balachandran K, Alford PW, Wylie-Sears J, Goss JA, Grosberg A, Bischoff J, Aikawa E, Levine RA, Parker KK. Cyclic strain induces dual-mode endothelial-mesenchymal transformation of the cardiac valve. Proc Natl Acad Sci U S A. 2011 Dec 13; 108(50):19943-8.
  25. Lu L, Bischoff J, Mulliken JB, Bielenberg DR, Fishman SJ, Greene AK. Increased endothelial progenitor cells and vasculogenic factors in higher-staged arteriovenous malformations. Plast Reconstr Surg. 2011 Oct; 128(4):260e-269e.
  26. Boscolo E, Mulliken JB, Bischoff J. VEGFR-1 mediates endothelial differentiation and formation of blood vessels in a murine model of infantile hemangioma. Am J Pathol. 2011 Nov; 179(5):2266-77.
  27. Greenberger S, Bischoff J. Infantile hemangioma-mechanism(s) of drug action on a vascular tumor. Cold Spring Harb Perspect Med. 2011 Sep; 1(1):a006460.
  28. Adepoju O, Wong A, Kitajewski A, Tong K, Boscolo E, Bischoff J, Kitajewski J, Wu JK. Expression of HES and HEY genes in infantile hemangiomas. Vasc Cell. 2011 Aug 11; 3:19.
  29. Bischoff J, Aikawa E. Progenitor cells confer plasticity to cardiac valve endothelium. J Cardiovasc Transl Res. 2011 Dec; 4(6):710-9.
  30. Wylie-Sears J, Aikawa E, Levine RA, Yang JH, Bischoff J. Mitral valve endothelial cells with osteogenic differentiation potential. Arterioscler Thromb Vasc Biol. 2011 Mar; 31(3):598-607.
  31. Sales VL, Mettler BA, Engelmayr GC, Aikawa E, Bischoff J, Martin DP, Exarhopoulos A, Moses MA, Schoen FJ, Sacks MS, Mayer JE. Endothelial progenitor cells as a sole source for ex vivo seeding of tissue-engineered heart valves. Tissue Eng Part A. 2010 Jan; 16(1):257-67.
  32. Elmasri H, Karaaslan C, Teper Y, Ghelfi E, Weng M, Ince TA, Kozakewich H, Bischoff J, Cataltepe S. Fatty acid binding protein 4 is a target of VEGF and a regulator of cell proliferation in endothelial cells. FASEB J. 2009 Nov; 23(11):3865-73.
  33. Mettler BA, Sales VL, Stucken CL, Anttila V, Mendelson K, Bischoff J, Mayer JE. Stem cell-derived, tissue-engineered pulmonary artery augmentation patches in vivo. Ann Thorac Surg. 2008 Jul; 86(1):132-40; discussion 140-1.
  34. Bischoff J, Griffioen AW. In memoriam Dr. Judah Folkman. Angiogenesis. 2008; 11(1):1-2.
  35. Melero-Martin JM, Bischoff J. Chapter 13. An in vivo experimental model for postnatal vasculogenesis. Methods Enzymol. 2008; 445:303-29.
  36. Mulliken JB, Bischoff J, Kozakewich HP. Multifocal rapidly involuting congenital hemangioma: a link to chorangioma. Am J Med Genet A. 2007 Dec 15; 143A(24):3038-46.
  37. Melero-Martin JM, Khan ZA, Picard A, Wu X, Paruchuri S, Bischoff J. In vivo vasculogenic potential of human blood-derived endothelial progenitor cells. Blood. 2007 Jun 1; 109(11):4761-8.
  38. Khan ZA, Melero-Martin JM, Wu X, Paruchuri S, Boscolo E, Mulliken JB, Bischoff J. Endothelial progenitor cells from infantile hemangioma and umbilical cord blood display unique cellular responses to endostatin. Blood. 2006 Aug 1; 108(3):915-21.
  39. Amiel GE, Komura M, Shapira O, Yoo JJ, Yazdani S, Berry J, Kaushal S, Bischoff J, Atala A, Soker S. Engineering of blood vessels from acellular collagen matrices coated with human endothelial cells. Tissue Eng. 2006 Aug; 12(8):2355-65.
  40. Yu Y, Fuhr J, Boye E, Gyorffy S, Soker S, Atala A, Mulliken JB, Bischoff J. Mesenchymal stem cells and adipogenesis in hemangioma involution. Stem Cells. 2006 Jun; 24(6):1605-12.
  41. Frieden IJ, Haggstrom AN, Drolet BA, Mancini AJ, Friedlander SF, Boon L, Chamlin SL, Baselga E, Garzon MC, Nopper AJ, Siegel DH, Mathes EW, Goddard DS, Bischoff J, North PE, Esterly NB. Infantile hemangiomas: current knowledge, future directions. Proceedings of a research workshop on infantile hemangiomas, April 7-9, 2005, Bethesda, Maryland, USA. Pediatr Dermatol. 2005 Sep-Oct; 22(5):383-406.
  42. Yu Y, Wylie-Sears J, Boscolo E, Mulliken JB, Bischoff J. Genomic imprinting of IGF2 is maintained in infantile hemangioma despite its high level of expression. Mol Med. 2004 Jul-Dec; 10(7-12):117-23.
  43. Yu Y, Moulton KS, Khan MK, Vineberg S, Boye E, Davis VM, O'Donnell PE, Bischoff J, Milstone DS. E-selectin is required for the antiangiogenic activity of endostatin. Proc Natl Acad Sci U S A. 2004 May 25; 101(21):8005-10.
  44. Li Q, Yu Y, Bischoff J, Mulliken JB, Olsen BR. Differential expression of CD146 in tissues and endothelial cells derived from infantile haemangioma and normal human skin. J Pathol. 2003 Oct; 201(2):296-302.
  45. Perry TE, Kaushal S, Sutherland FW, Guleserian KJ, Bischoff J, Sacks M, Mayer JE. Thoracic Surgery Directors Association Award. Bone marrow as a cell source for tissue engineering heart valves. Ann Thorac Surg. 2003 Mar; 75(3):761-7; discussion 767.
  46. Bischoff J. Monoclonal expansion of endothelial cells in hemangioma: an intrinsic defect with extrinsic consequences? Trends Cardiovasc Med. 2002 Jul; 12(5):220-4.
  47. Yu Y, Varughese J, Brown LF, Mulliken JB, Bischoff J. Increased Tie2 expression, enhanced response to angiopoietin-1, and dysregulated angiopoietin-2 expression in hemangioma-derived endothelial cells. Am J Pathol. 2001 Dec; 159(6):2271-80.
  48. Boye E, Yu Y, Paranya G, Mulliken JB, Olsen BR, Bischoff J. Clonality and altered behavior of endothelial cells from hemangiomas. J Clin Invest. 2001 Mar; 107(6):745-52.
  49. Luo J, Kato M, Wang H, Bernfield M, Bischoff J. Heparan sulfate and chondroitin sulfate proteoglycans inhibit E-selectin binding to endothelial cells. J Cell Biochem. 2001; 80(4):522-31.
  50. Tomlinson J, Wang JL, Barsky SH, Lee MC, Bischoff J, Nguyen M. Human colon cancer cells express multiple glycoprotein ligands for E-selectin. Int J Oncol. 2000 Feb; 16(2):347-53.
  51. Smith LE, Shen W, Perruzzi C, Soker S, Kinose F, Xu X, Robinson G, Driver S, Bischoff J, Zhang B, Schaeffer JM, Senger DR. Regulation of vascular endothelial growth factor-dependent retinal neovascularization by insulin-like growth factor-1 receptor. Nat Med. 1999 Dec; 5(12):1390-5.
  52. Luo J, Paranya G, Bischoff J. Noninflammatory expression of E-selectin is regulated by cell growth. Blood. 1999 Jun 1; 93(11):3785-91.
  53. Kräling BM, Bischoff J. A simplified method for growth of human microvascular endothelial cells results in decreased senescence and continued responsiveness to cytokines and growth factors. In Vitro Cell Dev Biol Anim. 1998 Apr; 34(4):308-15.
  54. Razon MJ, Kräling BM, Mulliken JB, Bischoff J. Increased apoptosis coincides with onset of involution in infantile hemangioma. Microcirculation. 1998; 5(2-3):189-95.
  55. Bischoff J. Cell adhesion and angiogenesis. J Clin Invest. 1997 Dec 1; 100(11 Suppl):S37-9.
  56. Bischoff J, Brasel C, Kräling B, Vranovska K. E-selectin is upregulated in proliferating endothelial cells in vitro. Microcirculation. 1997 Jun; 4(2):279-87.
  57. Bischoff J. Cell adhesion and angiogenesis. J Clin Invest. 1997 Feb 1; 99(3):373-6.
  58. Zünd G, Nelson DP, Neufeld EJ, Dzus AL, Bischoff J, Mayer JE, Colgan SP. Hypoxia enhances stimulus-dependent induction of E-selectin on aortic endothelial cells. Proc Natl Acad Sci U S A. 1996 Jul 9; 93(14):7075-80.
  59. Kräling BM, Razon MJ, Boon LM, Zurakowski D, Seachord C, Darveau RP, Mulliken JB, Corless CL, Bischoff J. E-selectin is present in proliferating endothelial cells in human hemangiomas. Am J Pathol. 1996 Apr; 148(4):1181-91.
  60. Bischoff J, Brasel C. Regulation of P-selectin by tumor necrosis factor-alpha. Biochem Biophys Res Commun. 1995 May 5; 210(1):174-80.
  61. Bischoff J. Approaches to studying cell adhesion molecules in angiogenesis. Trends Cell Biol. 1995 Feb; 5(2):69-74.
  62. Nguyen M, Strubel NA, Bischoff J. A role for sialyl Lewis-X/A glycoconjugates in capillary morphogenesis. Nature. 1993 Sep 16; 365(6443):267-9.
  63. Strubel NA, Nguyen M, Kansas GS, Tedder TF, Bischoff J. Isolation and characterization of a bovine cDNA encoding a functional homolog of human P-selectin. Biochem Biophys Res Commun. 1993 Apr 30; 192(2):338-44.
  64. Nguyen M, Folkman J, Bischoff J. 1-Deoxymannojirimycin inhibits capillary tube formation in vitro. Analysis of N-linked oligosaccharides in bovine capillary endothelial cells. J Biol Chem. 1992 Dec 25; 267(36):26157-65.
  65. Bischoff J, Moremen K, Lodish HF. Isolation, characterization, and expression of cDNA encoding a rat liver endoplasmic reticulum alpha-mannosidase. J Biol Chem. 1990 Oct 5; 265(28):17110-7.
  66. Bischoff J, Libresco S, Shia MA, Lodish HF. The H1 and H2 polypeptides associate to form the asialoglycoprotein receptor in human hepatoma cells. J Cell Biol. 1988 Apr; 106(4):1067-74.
  67. Paruchuri S, Yang JH, Aikawa E, Melero-Martin JM, Khan ZA, Loukogeorgakis S, Schoen FJ, Bischoff J. Human pulmonary valve progenitor cells exhibit endothelial/mesenchymal plasticity in response to vascular endothelial growth factor-A and transforming growth factor-beta2. Circ Res. 2006 Oct 13; 99(8):861-9.
  68. Melero-Martin JM, De Obaldia ME, Kang SY, Khan ZA, Yuan L, Oettgen P, Bischoff J. Engineering robust and functional vascular networks in vivo with human adult and cord blood-derived progenitor cells. Circ Res. 2008 Jul 18; 103(2):194-202.
  69. Allen P, Melero-Martin J, Bischoff J. Type I collagen, fibrin and PuraMatrix matrices provide permissive environments for human endothelial and mesenchymal progenitor cells to form neovascular networks. J Tissue Eng Regen Med. 2011 Apr; 5(4):e74-86.
  70. Dudley AC, Khan ZA, Shih SC, Kang SY, Zwaans BM, Bischoff J, Klagsbrun M. Calcification of multipotent prostate tumor endothelium. Cancer Cell. 2008 Sep 9; 14(3):201-11.
  71. Dvorin EL, Jacobson J, Roth SJ, Bischoff J. Human pulmonary valve endothelial cells express functional adhesion molecules for leukocytes. J Heart Valve Dis. 2003 Sep; 12(5):617-24.
  72. Wu X, Lensch MW, Wylie-Sears J, Daley GQ, Bischoff J. Hemogenic endothelial progenitor cells isolated from human umbilical cord blood. Stem Cells. 2007 Nov; 25(11):2770-6.
  73. Armstrong EJ, Bischoff J. Heart valve development: endothelial cell signaling and differentiation. Circ Res. 2004 Sep 3; 95(5):459-70.
  74. Greenberger S, Yuan S, Walsh LA, Boscolo E, Kang KT, Matthews B, Mulliken JB, Bischoff J. Rapamycin suppresses self-renewal and vasculogenic potential of stem cells isolated from infantile hemangioma. J Invest Dermatol. 2011 Dec; 131(12):2467-76.
  75. Lee YM, Cope JJ, Ackermann GE, Goishi K, Armstrong EJ, Paw BH, Bischoff J. Vascular endothelial growth factor receptor signaling is required for cardiac valve formation in zebrafish. Dev Dyn. 2006 Jan; 235(1):29-37.
  76. Bischoff J. Progenitor cells in infantile hemangioma. J Craniofac Surg. 2009 Mar; 20 Suppl 1:695-7.
  77. Wu JK, Adepoju O, De Silva D, Baribault K, Boscolo E, Bischoff J, Kitajewski J. A switch in Notch gene expression parallels stem cell to endothelial transition in infantile hemangioma. Angiogenesis. 2010 Mar; 13(1):15-23.
  78. Paranya G, Vineberg S, Dvorin E, Kaushal S, Roth SJ, Rabkin E, Schoen FJ, Bischoff J. Aortic valve endothelial cells undergo transforming growth factor-beta-mediated and non-transforming growth factor-beta-mediated transdifferentiation in vitro. Am J Pathol. 2001 Oct; 159(4):1335-43.
  79. Luo J, Lin J, Paranya G, Bischoff J. Angiostatin upregulates E-selectin in proliferating endothelial cells. Biochem Biophys Res Commun. 1998 Apr 28; 245(3):906-11.
  80. Khan ZA, Boscolo E, Picard A, Psutka S, Melero-Martin JM, Bartch TC, Mulliken JB, Bischoff J. Multipotential stem cells recapitulate human infantile hemangioma in immunodeficient mice. J Clin Invest. 2008 Jul; 118(7):2592-9.
  81. Yu Y, Flint A, Dvorin EL, Bischoff J. AC133-2, a novel isoform of human AC133 stem cell antigen. J Biol Chem. 2002 Jun 7; 277(23):20711-6.
  82. Jang GH, Park IS, Yang JH, Bischoff J, Lee YM. Differential functions of genes regulated by VEGF-NFATc1 signaling pathway in the migration of pulmonary valve endothelial cells. FEBS Lett. 2010 Jan 4; 584(1):141-6.
  83. Yu Y, Flint AF, Mulliken JB, Wu JK, Bischoff J. Endothelial progenitor cells in infantile hemangioma. Blood. 2004 Feb 15; 103(4):1373-5.
  84. Wu X, Rabkin-Aikawa E, Guleserian KJ, Perry TE, Masuda Y, Sutherland FW, Schoen FJ, Mayer JE, Bischoff J. Tissue-engineered microvessels on three-dimensional biodegradable scaffolds using human endothelial progenitor cells. Am J Physiol Heart Circ Physiol. 2004 Aug; 287(2):H480-7.
  85. Greenberger S, Boscolo E, Adini I, Mulliken JB, Bischoff J. Corticosteroid suppression of VEGF-A in infantile hemangioma-derived stem cells. N Engl J Med. 2010 Mar 18; 362(11):1005-13.
  86. Yang JH, Wylie-Sears J, Bischoff J. Opposing actions of Notch1 and VEGF in post-natal cardiac valve endothelial cells. Biochem Biophys Res Commun. 2008 Sep 26; 374(3):512-6.
  87. Boscolo E, Bischoff J. Vasculogenesis in infantile hemangioma. Angiogenesis. 2009; 12(2):197-207.
  88. Hjortnaes J, Gottlieb D, Figueiredo JL, Melero-Martin J, Kohler RH, Bischoff J, Weissleder R, Mayer JE, Aikawa E. Intravital molecular imaging of small-diameter tissue-engineered vascular grafts in mice: a feasibility study. Tissue Eng Part C Methods. 2010 Aug; 16(4):597-607.
  89. Kang KT, Allen P, Bischoff J. Bioengineered human vascular networks transplanted into secondary mice reconnect with the host vasculature and re-establish perfusion. Blood. 2011 Dec 15; 118(25):6718-21.
  90. Picard A, Boscolo E, Khan ZA, Bartch TC, Mulliken JB, Vazquez MP, Bischoff J. IGF-2 and FLT-1/VEGF-R1 mRNA levels reveal distinctions and similarities between congenital and common infantile hemangioma. Pediatr Res. 2008 Mar; 63(3):263-7.
  91. Kaushal S, Amiel GE, Guleserian KJ, Shapira OM, Perry T, Sutherland FW, Rabkin E, Moran AM, Schoen FJ, Atala A, Soker S, Bischoff J, Mayer JE. Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo. Nat Med. 2001 Sep; 7(9):1035-40.
  92. Johnson EN, Lee YM, Sander TL, Rabkin E, Schoen FJ, Kaushal S, Bischoff J. NFATc1 mediates vascular endothelial growth factor-induced proliferation of human pulmonary valve endothelial cells. J Biol Chem. 2003 Jan 17; 278(3):1686-92.
  93. Dal-Bianco JP, Aikawa E, Bischoff J, Guerrero JL, Handschumacher MD, Sullivan S, Johnson B, Titus JS, Iwamoto Y, Wylie-Sears J, Levine RA, Carpentier A. Active adaptation of the tethered mitral valve: insights into a compensatory mechanism for functional mitral regurgitation. Circulation. 2009 Jul 28; 120(4):334-42.
  94. Boscolo E, Stewart CL, Greenberger S, Wu JK, Durham JT, Herman IM, Mulliken JB, Kitajewski J, Bischoff J. JAGGED1 signaling regulates hemangioma stem cell-to-pericyte/vascular smooth muscle cell differentiation. Arterioscler Thromb Vasc Biol. 2011 Oct; 31(10):2181-92.
  95. Melero-Martin JM, De Obaldia ME, Allen P, Dudley AC, Klagsbrun M, Bischoff J. Host myeloid cells are necessary for creating bioengineered human vascular networks in vivo. Tissue Eng Part A. 2010 Aug; 16(8):2457-66.
  96. Dvorin EL, Wylie-Sears J, Kaushal S, Martin DP, Bischoff J. Quantitative evaluation of endothelial progenitors and cardiac valve endothelial cells: proliferation and differentiation on poly-glycolic acid/poly-4-hydroxybutyrate scaffold in response to vascular endothelial growth factor and transforming growth factor beta1. Tissue Eng. 2003 Jun; 9(3):487-93.
  97. Budson AE, Ko L, Brasel C, Bischoff J. The angiogenesis inhibitor AGM-1470 selectively increases E-selectin. Biochem Biophys Res Commun. 1996 Aug 5; 225(1):141-5.
  98. Greenberger S, Adini I, Boscolo E, Mulliken JB, Bischoff J. Targeting NF-?B in infantile hemangioma-derived stem cells reduces VEGF-A expression. Angiogenesis. 2010 Dec; 13(4):327-35.
  99. Jinnin M, Medici D, Park L, Limaye N, Liu Y, Boscolo E, Bischoff J, Vikkula M, Boye E, Olsen BR. Suppressed NFAT-dependent VEGFR1 expression and constitutive VEGFR2 signaling in infantile hemangioma. Nat Med. 2008 Nov; 14(11):1236-46.
  100. Kang KT, Coggins M, Xiao C, Rosenzweig A, Bischoff J. Human vasculogenic cells form functional blood vessels and mitigate adverse remodeling after ischemia reperfusion injury in rats. Angiogenesis. 2013 Oct; 16(4):773-84.
  101. Sawada N, Jiang A, Takizawa F, Safdar A, Manika A, Tesmenitsky Y, Kang KT, Bischoff J, Kalwa H, Sartoretto JL, Kamei Y, Benjamin LE, Watada H, Ogawa Y, Higashikuni Y, Kessinger CW, Jaffer FA, Michel T, Sata M, Croce K, Tanaka R, Arany Z. Endothelial PGC-1a mediates vascular dysfunction in diabetes. Cell Metab. 2014 Feb 4; 19(2):246-58.
  102. Jia D, Huang L, Bischoff J, Moses MA. The endogenous zinc finger transcription factor, ZNF24, modulates the angiogenic potential of human microvascular endothelial cells. FASEB J. 2015 Apr; 29(4):1371-82.
  103. Nakayama H, Huang L, Kelly RP, Oudenaarden CR, Dagher A, Hofmann NA, Moses MA, Bischoff J, Klagsbrun M. Infantile hemangioma-derived stem cells and endothelial cells are inhibited by class 3 semaphorins. Biochem Biophys Res Commun. 2015 Aug 14; 464(1):126-32.
  104. Dal-Bianco JP, Aikawa E, Bischoff J, Guerrero JL, Hjortnaes J, Beaudoin J, Szymanski C, Bartko PE, Seybolt MM, Handschumacher MD, Sullivan S, Garcia ML, Mauskapf A, Titus JS, Wylie-Sears J, Irvin WS, Chaput M, Messas E, Hagège AA, Carpentier A, Levine RA. Myocardial Infarction Alters Adaptation of the Tethered Mitral Valve. J Am Coll Cardiol. 2016 Jan 26; 67(3):275-87.
  105. Ayturk UM, Couto JA, Hann S, Mulliken JB, Williams KL, Huang AY, Fishman SJ, Boyd TK, Kozakewich HP, Bischoff J, Greene AK, Warman ML. Somatic Activating Mutations in GNAQ and GNA11 Are Associated with Congenital Hemangioma. Am J Hum Genet. 2016 Apr 7; 98(4):789-95.
  106. Boscolo E, Limaye N, Huang L, Kang KT, Soblet J, Uebelhoer M, Mendola A, Natynki M, Seront E, Dupont S, Hammer J, Legrand C, Brugnara C, Eklund L, Vikkula M, Bischoff J, Boon LM. Rapamycin improves TIE2-mutated venous malformation in murine model and human subjects. J Clin Invest. 2015 Sep; 125(9):3491-504.
  107. Rossi E, Smadja DM, Boscolo E, Langa C, Arevalo MA, Pericacho M, Gamella-Pozuelo L, Kauskot A, Botella LM, Gaussem P, Bischoff J, Lopez-Novoa JM, Bernabeu C. Endoglin regulates mural cell adhesion in the circulatory system. Cell Mol Life Sci. 2016 Apr; 73(8):1715-39.
  108. Bai S, Ingram P, Chen YC, Deng N, Pearson A, Niknafs Y, O'Hayer P, Wang Y, Zhang ZY, Boscolo E, Bischoff J, Yoon E, Buckanovich RJ. EGFL6 Regulates the Asymmetric Division, Maintenance, and Metastasis of ALDH+ Ovarian Cancer Cells. Cancer Res. 2016 Nov 1; 76(21):6396-6409.
LIke ThisLIke ThisLIke This

Related Laboratory

Bischoff Laboratory

Learn more
Close