Research

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John Froehlich, PhD

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Department:
Urology Research
Hospital Title:
Staff Sceintist
Academic Title:
Research Fellow
Research Focus Area:
Urinary proteomicsGlycomicsGlycoproteomicsMass Spectrometry
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Publications

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  1. Bennike T, Ayturk U, Haslauer CM, Froehlich JW, Proffen BL, Barnaby O, Birkelund S, Murray MM, Warman ML, Stensballe A, Steen H. A normative study of the synovial fluid proteome from healthy porcine knee joints. J Proteome Res. 2014 Oct 3; 13(10):4377-87.
  2. Zhou H, Warren PG, Froehlich JW, Lee RS. Dual modifications strategy to quantify neutral and sialylated N-glycans simultaneously by MALDI-MS. Anal Chem. 2014 Jul 1; 86(13):6277-84.
  3. Zhou H, Briscoe AC, Froehlich JW, Lee RS. PNGase F catalyzes de-N-glycosylation in a domestic microwave. Anal Biochem. 2012 Aug 1; 427(1):33-5.
  4. Froehlich JW, Barboza M, Chu C, Lerno LA, Clowers BH, Zivkovic AM, German JB, Lebrilla CB. Nano-LC-MS/MS of glycopeptides produced by nonspecific proteolysis enables rapid and extensive site-specific glycosylation determination. Anal Chem. 2011 Jul 15; 83(14):5541-7.
  5. Froehlich JE, Wilkerson CG, Ray WK, McAndrew RS, Osteryoung KW, Gage DA, Phinney BS. Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis. J Proteome Res. 2003 Jul-Aug; 2(4):413-25.
  6. Zhou H, Froehlich JW, Briscoe AC, Lee RS. The GlycoFilter: a simple and comprehensive sample preparation platform for proteomics, N-glycomics and glycosylation site assignment. Mol Cell Proteomics. 2013 Oct; 12(10):2981-91.
  7. Froehlich JW, Chu CS, Tang N, Waddell K, Grimm R, Lebrilla CB. Label-free liquid chromatography-tandem mass spectrometry analysis with automated phosphopeptide enrichment reveals dynamic human milk protein phosphorylation during lactation. Anal Biochem. 2011 Jan 1; 408(1):136-46.
  8. Froehlich JW, Dodds ED, Barboza M, McJimpsey EL, Seipert RR, Francis J, An HJ, Freeman S, German JB, Lebrilla CB. Glycoprotein expression in human milk during lactation. J Agric Food Chem. 2010 May 26; 58(10):6440-8.
  9. Barboza M, Pinzon J, Wickramasinghe S, Froehlich JW, Moeller I, Smilowitz JT, Ruhaak LR, Huang J, Lönnerdal B, German JB, Medrano JF, Weimer BC, Lebrilla CB. Glycosylation of human milk lactoferrin exhibits dynamic changes during early lactation enhancing its role in pathogenic bacteria-host interactions. Mol Cell Proteomics. 2012 Jun; 11(6):M111.015248.
  10. Serang O, Froehlich JW, Muntel J, McDowell G, Steen H, Lee RS, Steen JA. SweetSEQer, simple de novo filtering and annotation of glycoconjugate mass spectra. Mol Cell Proteomics. 2013 Jun; 12(6):1735-40.
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  12. Froehlich JW, Dodds ED, Wilhelm M, Serang O, Steen JA, Lee RS. A classifier based on accurate mass measurements to aid large scale, unbiased glycoproteomics. Mol Cell Proteomics. 2013 Apr; 12(4):1017-25.
  13. An HJ, Froehlich JW, Lebrilla CB. Determination of glycosylation sites and site-specific heterogeneity in glycoproteins. Curr Opin Chem Biol. 2009 Oct; 13(4):421-6.
  14. Froehlich JW, Vaezzadeh AR, Kirchner M, Briscoe AC, Hofmann O, Hide W, Steen H, Lee RS. An in-depth comparison of the male pediatric and adult urinary proteomes. Biochim Biophys Acta. 2014 May; 1844(5):1044-50.
  15. Froehlich JW, Kostel SA, Cho PS, Briscoe AC, Steen H, Vaezzadeh AR, Lee RS. Urinary Proteomics Yield Pathological Insights for Ureteropelvic Junction Obstruction. Mol Cell Proteomics. 2016 Aug; 15(8):2607-15.
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