Research

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Elizabeth Buttermore, PhD

Research Center:
Translational Neuroscience Center
Program:
Neurobiology Program
Department:
Neurology Research
Hospital Title:
Research Associate
Contact:
617-919-4377
Contact Via Email
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Overview

Research Focus Area(s)

* Phenotyping of human iPSC-derived neurons

Research Overview

My background is in neuronal cellular reprogramming and developmental neurobiology. I am currently leading phenotypic assay development with iPSC-derived human neurons in the Human Neuron Core at Boston Children's Hospital. Phenotypic assays that I use include, but are not limited to, functional (Axion MEA), biochemical (western blots), morphological (arrayscan analyses), and cell expression (Ion Torrent AmpliSeq whole transcriptome) data in high throughput and high content formats. Development and implementation of these assays within the Human Neuron Core in the Translational Neuroscience Center at Boston Children's Hospital will allow us to fully characterize patient-derived neurons with a well rounded suite of technology. These assays will also be utilized to develop medium to high throughput screens with the goal of identifying new tool compounds and pathways for targeting novel therapeutics for various neurodevelopmental and psychiatric disorders.

About the Researcher

I completed my postdoc in 2016 in Clifford Woolf's lab in the Kirby Neurobiology Center at Boston Children's Hospital. During my postdoc I worked to develop protocols for differentiating sensory neurons from iPSCs and fibroblasts. I then used these neurons to develop assays for chemotherapy induced neuropathy and chronic pain. Prior to my postdoc, I completed my PhD in 2012 in the lab of Manzoor Bhat at the University of North Carolina. My thesis focused on studying the organization and maintenance of molecular domains in myelinated axons. My BS degree comes from the University of Richmond where I graduated in 2006, having majored in Biochemistry and Molecular Biology.

1) Wainger and Buttermore, et al. 2015. Modeling pain in vitro using nociceptor neurons reprogrammed from fibroblasts. Nat Neurosci. 18(1):17-24. PMID:25420066

2) Buttermore, et al. 2013. Organization and maintenance of molecular domains in myelinated axons. J Neurosci Res. 91(5):603-22. PMID:23404451

3) Buttermore, et al. 2012. Pinceau organization in the cerebellum requires distinct functions of neurofascin in Purkinje and basket neurons during postnatal development. 32(14):4724-42. PMID:22492029

4) Buttermore, et al. 2011. The cytoskeletal adaptor protein band 4.1B is required for the maintenance of paranodal axoglial septate junctions in myelinated axons. J Neurosci. 31(22):8013-24. PMID:21632923

5) O'Brien et al., 2010. Localization of the paranodal protein Caspr in the mammalian retina. Mol Vis. 16:1854-63. PMID:21031018

6) Sousa, et al. 2009. The septate junction protein caspr is required for structural support and retention of KCNQ4 at calyceal synapses of vestibular hair cells. J Neurosci. 29(10):3103-8. PMID:19279247

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Translational Neuroscience Center

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