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Denisa  Wagner, PhD

Denisa Wagner PhD
Lab:
Research Center:
Dana-Farber/Boston Children’s Cancer and Blood Disorders Center
Program:
Program in Cellular and Molecular Medicine (PCMM)
Department:
Medicine Research
Division
Hematology/Oncology Research
Hospital Title:
Senior Investigator, Program in Cellular and Molecular Medicine
Academic Title:
Professor of Pediatrics, Harvard Medical School
Research Focus Area:
how blood cells respond rapidly to injury or stressful situations and initiate defensive or reparatory processes
Contact:
617-713-8300
Contact Via Email
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Research Overview

Visit The Wagner Lab

The Wagner lab studies how blood cells respond to injury or stressful situations and initiate defensive or reparatory processes. Specifically, they investigate the crucial role of cellular adhesion interactions in the response process, as well as the regulation of the adhesion molecules involved in normal physiology and in pathological situations.

Blood cells called platelets form a platelet plug to stop bleeding. Leukocytes (white blood cells) emigrate from the blood vessels to fight invading infectious agents and/or release biological mediators that stimulate the function of the surrounding organ to better respond to the demands of stress. All this requires activation of adhesive processes that allow vascular cells to bind to each other and to cells in the organs where the leukocytes emigrate.

To advance their work, Wagner and colleagues have engineered mice lacking platelet, endothelial, or leukocyte adhesion receptors. These animals permit modeling of human diseases with similar genetic defects and evaluation of the performance of mutant blood cells in live vessels through intravital microscopy. The Wagner lab studies platelet adhesion to the vessel wall, which can lead to thrombus formation and sometimes embolization. Recently, they observed that nuclear DNA ejected by stimulated leukocytes (NETs) greatly enhanced pathological thrombosis and organ injury during inflammation.

Wagner and colleagues also are researching the importance of adhesion molecules in inflammatory processes such as atherosclerosis, the narrowing of arteries. They have observed that leukocytes recruited to the liver help to regulate fat metabolism and help to prevent obesity. The lab began to visualize blood vessels in the brain and study mechanisms involved in the formation of the blood brain barrier, which prevents uncontrolled leakage of molecules from the blood into the central nervous system. They ask, do malfunctions of brain microcirculation contribute to the etiology of neurodegenerative diseases such as Alzheimer's? Using animal models, they are testing new ways to modulate blood brain barrier permeability and to evaluate inhibitors of adhesion molecules as possible agents to prevent heart disease and/or thrombosis.

About Denisa Wagner

Dr. Wagner received her Diploma of Biochemistry from the Université de Geneve, Switzerland and Ph.D. in Biology from the Massachusetts Institute of Technology. She served on the faculty at the University of Rochester, NY and Tufts University School of Medicine before coming to Harvard Medical School in 1994.  She is the Center for Blood Research Professor of Pediatrics, a senior investigator in the Program in Cellular and Molecular Medicine, and a member of the Division of Hematology/ Oncology at Boston Children's Hospital.

Publications

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  1. Pillai PS, Molony RD, Martinod K, Dong H, Pang IK, Tal MC, Solis AG, Bielecki P, Mohanty S, Trentalange M, Homer RJ, Flavell RA, Wagner DD, Montgomery RR, Shaw AC, Staeheli P, Iwasaki A. Mx1 reveals innate pathways to antiviral resistance and lethal influenza disease. Science. 2016 Apr 22; 352(6284):463-6.
  2. Demers M, Wong SL, Martinod K, Gallant M, Cabral JE, Wang Y, Wagner DD. Priming of neutrophils toward NETosis promotes tumor growth. Oncoimmunology. 2016 May; 5(5):e1134073.
  3. Thålin C, Demers M, Blomgren B, Wong SL, von Arbin M, von Heijne A, Laska AC, Wallén H, Wagner DD, Aspberg S. NETosis promotes cancer-associated arterial microthrombosis presenting as ischemic stroke with troponin elevation. Thromb Res. 2016 Mar; 139:56-64.
  4. Wong SL, Demers M, Martinod K, Gallant M, Wang Y, Goldfine AB, Kahn CR, Wagner DD. Diabetes primes neutrophils to undergo NETosis, which impairs wound healing. Nat Med. 2015 Jul; 21(7):815-9.
  5. Etulain J, Martinod K, Wong SL, Cifuni SM, Schattner M, Wagner DD. P-selectin promotes neutrophil extracellular trap formation in mice. Blood. 2015 Jul 9; 126(2):242-6.
  6. Martinod K, Fuchs TA, Zitomersky NL, Wong SL, Demers M, Gallant M, Wang Y, Wagner DD. PAD4-deficiency does not affect bacteremia in polymicrobial sepsis and ameliorates endotoxemic shock. Blood. 2015 Mar 19; 125(12):1948-56.
  7. Lewis HD, Liddle J, Coote JE, Atkinson SJ, Barker MD, Bax BD, Bicker KL, Bingham RP, Campbell M, Chen YH, Chung CW, Craggs PD, Davis RP, Eberhard D, Joberty G, Lind KE, Locke K, Maller C, Martinod K, Patten C, Polyakova O, Rise CE, Rüdiger M, Sheppard RJ, Slade DJ, Thomas P, Thorpe J, Yao G, Drewes G, Wagner DD, Thompson PR, Prinjha RK, Wilson DM. Inhibition of PAD4 activity is sufficient to disrupt mouse and human NET formation. Nat Chem Biol. 2015 Mar; 11(3):189-91.
  8. Reiner MF, Martinod K, Stivala S, Savarese G, Camici GG, Lüscher TF, Wagner DD, Beer JH. Dietary omega-3 alpha-linolenic acid does not prevent venous thrombosis in mice. Thromb Haemost. 2015 Jan; 113(1):177-84.
  9. Chen G, Zhang D, Fuchs TA, Manwani D, Wagner DD, Frenette PS. Heme-induced neutrophil extracellular traps contribute to the pathogenesis of sickle cell disease. Blood. 2014 Jun 12; 123(24):3818-27.
  10. Demers M, Wagner DD. NETosis: a new factor in tumor progression and cancer-associated thrombosis. Semin Thromb Hemost. 2014 Apr; 40(3):277-83.
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  12. Martinod K, Wagner DD. Thrombosis: tangled up in NETs. Blood. 2014 May 1; 123(18):2768-76.
  13. Suidan GL, Brill A, De Meyer SF, Voorhees JR, Cifuni SM, Cabral JE, Wagner DD. Endothelial Von Willebrand factor promotes blood-brain barrier flexibility and provides protection from hypoxia and seizures in mice. Arterioscler Thromb Vasc Biol. 2013 Sep; 33(9):2112-20.
  14. Martinod K, Demers M, Fuchs TA, Wong SL, Brill A, Gallant M, Hu J, Wang Y, Wagner DD. Neutrophil histone modification by peptidylarginine deiminase 4 is critical for deep vein thrombosis in mice. Proc Natl Acad Sci U S A. 2013 May 21; 110(21):8674-9.
  15. Fuchs TA, Alvarez JJ, Martinod K, Bhandari AA, Kaufman RM, Wagner DD. Neutrophils release extracellular DNA traps during storage of red blood cell units. Transfusion. 2013 Dec; 53(12):3210-6.
  16. Suidan GL, Duerschmied D, Dillon GM, Vanderhorst V, Hampton TG, Wong SL, Voorhees JR, Wagner DD. Lack of tryptophan hydroxylase-1 in mice results in gait abnormalities. PLoS One. 2013; 8(3):e59032.
  17. Demers M, Wagner DD. Neutrophil extracellular traps: A new link to cancer-associated thrombosis and potential implications for tumor progression. Oncoimmunology. 2013 Feb 1; 2(2):e22946.
  18. Duerschmied D, Suidan GL, Demers M, Herr N, Carbo C, Brill A, Cifuni SM, Mauler M, Cicko S, Bader M, Idzko M, Bode C, Wagner DD. Platelet serotonin promotes the recruitment of neutrophils to sites of acute inflammation in mice. Blood. 2013 Feb 7; 121(6):1008-15.
  19. Carbo C, Yuki K, Demers M, Wagner DD, Shimaoka M. Isoflurane inhibits neutrophil recruitment in the cutaneous Arthus reaction model. J Anesth. 2013 Apr; 27(2):261-8.
  20. Chen K, Nishi H, Travers R, Tsuboi N, Martinod K, Wagner DD, Stan R, Croce K, Mayadas TN. Endocytosis of soluble immune complexes leads to their clearance by Fc?RIIIB but induces neutrophil extracellular traps via Fc?RIIA in vivo. Blood. 2012 Nov 22; 120(22):4421-31.
  21. Demers M, Krause DS, Schatzberg D, Martinod K, Voorhees JR, Fuchs TA, Scadden DT, Wagner DD. Cancers predispose neutrophils to release extracellular DNA traps that contribute to cancer-associated thrombosis. Proc Natl Acad Sci U S A. 2012 Aug 7; 109(32):13076-81.
  22. Korin N, Kanapathipillai M, Matthews BD, Crescente M, Brill A, Mammoto T, Ghosh K, Jurek S, Bencherif SA, Bhatta D, Coskun AU, Feldman CL, Wagner DD, Ingber DE. Shear-activated nanotherapeutics for drug targeting to obstructed blood vessels. Science. 2012 Aug 10; 337(6095):738-42.
  23. Fuchs TA, Brill A, Wagner DD. Neutrophil extracellular trap (NET) impact on deep vein thrombosis. Arterioscler Thromb Vasc Biol. 2012 Aug; 32(8):1777-83.
  24. Brill A, Yesilaltay A, De Meyer SF, Kisucka J, Fuchs TA, Kocher O, Krieger M, Wagner DD. Extrahepatic high-density lipoprotein receptor SR-BI and apoA-I protect against deep vein thrombosis in mice. Arterioscler Thromb Vasc Biol. 2012 Aug; 32(8):1841-7.
  25. Fuchs TA, Kremer Hovinga JA, Schatzberg D, Wagner DD, Lämmle B. Circulating DNA and myeloperoxidase indicate disease activity in patients with thrombotic microangiopathies. Blood. 2012 Aug 9; 120(6):1157-64.
  26. Thomas GM, Carbo C, Curtis BR, Martinod K, Mazo IB, Schatzberg D, Cifuni SM, Fuchs TA, von Andrian UH, Hartwig JH, Aster RH, Wagner DD. Extracellular DNA traps are associated with the pathogenesis of TRALI in humans and mice. Blood. 2012 Jun 28; 119(26):6335-43.
  27. von Brühl ML, Stark K, Steinhart A, Chandraratne S, Konrad I, Lorenz M, Khandoga A, Tirniceriu A, Coletti R, Köllnberger M, Byrne RA, Laitinen I, Walch A, Brill A, Pfeiler S, Manukyan D, Braun S, Lange P, Riegger J, Ware J, Eckart A, Haidari S, Rudelius M, Schulz C, Echtler K, Brinkmann V, Schwaiger M, Preissner KT, Wagner DD, Mackman N, Engelmann B, Massberg S. Monocytes, neutrophils, and platelets cooperate to initiate and propagate venous thrombosis in mice in vivo. J Exp Med. 2012 Apr 9; 209(4):819-35.
  28. Siller-Matula JM, Merhi Y, Tanguay JF, Duerschmied D, Wagner DD, McGinness KE, Pendergrast PS, Chung JK, Tian X, Schaub RG, Jilma B. ARC15105 is a potent antagonist of von Willebrand factor mediated platelet activation and adhesion. Arterioscler Thromb Vasc Biol. 2012 Apr; 32(4):902-9.
  29. Brill A, Fuchs TA, Savchenko AS, Thomas GM, Martinod K, De Meyer SF, Bhandari AA, Wagner DD. Neutrophil extracellular traps promote deep vein thrombosis in mice. J Thromb Haemost. 2012 Jan; 10(1):136-44.
  30. Demers M, Wagner DD. Targeting platelet function to improve drug delivery. Oncoimmunology. 2012 Jan 1; 1(1):100-102.
  31. De Meyer SF, Schwarz T, Schatzberg D, Wagner DD. Platelet glycoprotein Iba is an important mediator of ischemic stroke in mice. Exp Transl Stroke Med. 2011 Sep 13; 3:9.
  32. Fuchs TA, Bhandari AA, Wagner DD. Histones induce rapid and profound thrombocytopenia in mice. Blood. 2011 Sep 29; 118(13):3708-14.
  33. Flick MJ, Chauhan AK, Frederick M, Talmage KE, Kombrinck KW, Miller W, Mullins ES, Palumbo JS, Zheng X, Esmon NL, Esmon CT, Thornton S, Becker A, Pelc LA, Di Cera E, Wagner DD, Degen JL. The development of inflammatory joint disease is attenuated in mice expressing the anticoagulant prothrombin mutant W215A/E217A. Blood. 2011 Jun 9; 117(23):6326-37.
  34. Brill A, Fuchs TA, Chauhan AK, Yang JJ, De Meyer SF, Köllnberger M, Wakefield TW, Lämmle B, Massberg S, Wagner DD. von Willebrand factor-mediated platelet adhesion is critical for deep vein thrombosis in mouse models. Blood. 2011 Jan 27; 117(4):1400-7.
  35. Fuchs TA, Brill A, Duerschmied D, Schatzberg D, Monestier M, Myers DD, Wrobleski SK, Wakefield TW, Hartwig JH, Wagner DD. Extracellular DNA traps promote thrombosis. Proc Natl Acad Sci U S A. 2010 Sep 7; 107(36):15880-5.
  36. Carbo C, Duerschmied D, Goerge T, Hattori H, Sakai J, Cifuni SM, White GC, Chrzanowska-Wodnicka M, Luo HR, Wagner DD. Integrin-independent role of CalDAG-GEFI in neutrophil chemotaxis. J Leukoc Biol. 2010 Aug; 88(2):313-9.
  37. Ho-Tin-Noé B, Carbo C, Demers M, Cifuni SM, Goerge T, Wagner DD. Innate immune cells induce hemorrhage in tumors during thrombocytopenia. Am J Pathol. 2009 Oct; 175(4):1699-708.
  38. Ho-Tin-Noé B, Goerge T, Wagner DD. Platelets: guardians of tumor vasculature. Cancer Res. 2009 Jul 15; 69(14):5623-6.
  39. Bernimoulin M, Waters EK, Foy M, Steele BM, Sullivan M, Falet H, Walsh MT, Barteneva N, Geng JG, Hartwig JH, Maguire PB, Wagner DD. Differential stimulation of monocytic cells results in distinct populations of microparticles. J Thromb Haemost. 2009 Jun; 7(6):1019-28.
  40. Diener JL, Daniel Lagassé HA, Duerschmied D, Merhi Y, Tanguay JF, Hutabarat R, Gilbert J, Wagner DD, Schaub R. Inhibition of von Willebrand factor-mediated platelet activation and thrombosis by the anti-von Willebrand factor A1-domain aptamer ARC1779. J Thromb Haemost. 2009 Jul; 7(7):1155-62.
  41. Kisucka J, Chauhan AK, Zhao BQ, Patten IS, Yesilaltay A, Krieger M, Wagner DD. Elevated levels of soluble P-selectin in mice alter blood-brain barrier function, exacerbate stroke, and promote atherosclerosis. Blood. 2009 Jun 4; 113(23):6015-22.
  42. Lu F, Chauhan AK, Fernandes SM, Walsh MT, Wagner DD, Davis AE. The effect of C1 inhibitor on intestinal ischemia and reperfusion injury. Am J Physiol Gastrointest Liver Physiol. 2008 Nov; 295(5):G1042-9.
  43. Kisucka J, Chauhan AK, Patten IS, Yesilaltay A, Neumann C, Van Etten RA, Krieger M, Wagner DD. Peroxiredoxin1 prevents excessive endothelial activation and early atherosclerosis. Circ Res. 2008 Sep 12; 103(6):598-605.
  44. Noubade R, del Rio R, McElvany B, Zachary JF, Millward JM, Wagner DD, Offner H, Blankenhorn EP, Teuscher C. von-Willebrand factor influences blood brain barrier permeability and brain inflammation in experimental allergic encephalomyelitis. Am J Pathol. 2008 Sep; 173(3):892-900.
  45. Cifuni SM, Wagner DD, Bergmeier W. CalDAG-GEFI and protein kinase C represent alternative pathways leading to activation of integrin alphaIIbbeta3 in platelets. Blood. 2008 Sep 1; 112(5):1696-703.
  46. Wagner DD, Frenette PS. The vessel wall and its interactions. Blood. 2008 Jun 1; 111(11):5271-81.
  47. Goerge T, Ho-Tin-Noe B, Carbo C, Benarafa C, Remold-O'Donnell E, Zhao BQ, Cifuni SM, Wagner DD. Inflammation induces hemorrhage in thrombocytopenia. Blood. 2008 May 15; 111(10):4958-64.
  48. Dole VS, Bergmeier W, Patten IS, Hirahashi J, Mayadas TN, Wagner DD. PSGL-1 regulates platelet P-selectin-mediated endothelial activation and shedding of P-selectin from activated platelets. Thromb Haemost. 2007 Oct; 98(4):806-12.
  49. Junt T, Schulze H, Chen Z, Massberg S, Goerge T, Krueger A, Wagner DD, Graf T, Italiano JE, Shivdasani RA, von Andrian UH. Dynamic visualization of thrombopoiesis within bone marrow. Science. 2007 Sep 21; 317(5845):1767-70.
  50. Bergmeier W, Goerge T, Wang HW, Crittenden JR, Baldwin AC, Cifuni SM, Housman DE, Graybiel AM, Wagner DD. Mice lacking the signaling molecule CalDAG-GEFI represent a model for leukocyte adhesion deficiency type III. J Clin Invest. 2007 Jun; 117(6):1699-707.
  51. Krüger K, Wagner D, Gawenda M, Strohe D, Uedelhoven J, Brunkwall J, Lackner K. [Coil embolization of arteriovenous fistulae on in situ saphenous vein bypasses: success rate and complications]. Rofo. 2007 Jun; 179(6):587-92.
  52. Babic AM, Josefsson EC, Bergmeier W, Wagner DD, Kaufman RM, Silberstein LE, Stossel TP, Hartwig JH, Hoffmeister KM. In vitro function and phagocytosis of galactosylated platelet concentrates after long-term refrigeration. Transfusion. 2007 Mar; 47(3):442-51.
  53. Denis CV, Wagner DD. Platelet adhesion receptors and their ligands in mouse models of thrombosis. Arterioscler Thromb Vasc Biol. 2007 Apr; 27(4):728-39.
  54. Chauhan AK, Kisucka J, Lamb CB, Bergmeier W, Wagner DD. von Willebrand factor and factor VIII are independently required to form stable occlusive thrombi in injured veins. Blood. 2007 Mar 15; 109(6):2424-9.
  55. Hafezi-Moghadam A, Thomas KL, Wagner DD. ApoE deficiency leads to a progressive age-dependent blood-brain barrier leakage. Am J Physiol Cell Physiol. 2007 Apr; 292(4):C1256-62.
  56. Yang H, Reheman A, Chen P, Zhu G, Hynes RO, Freedman J, Wagner DD, Ni H. Fibrinogen and von Willebrand factor-independent platelet aggregation in vitro and in vivo. J Thromb Haemost. 2006 Oct; 4(10):2230-7.
  57. Yang D, Zhang Y, Nguyen HG, Koupenova M, Chauhan AK, Makitalo M, Jones MR, St Hilaire C, Seldin DC, Toselli P, Lamperti E, Schreiber BM, Gavras H, Wagner DD, Ravid K. The A2B adenosine receptor protects against inflammation and excessive vascular adhesion. J Clin Invest. 2006 Jul; 116(7):1913-23.
  58. Kirkpatrick BD, Huston CD, Wagner D, Noel F, Rouzier P, Pape JW, Bois G, Larsson CJ, Alston WK, Tenney K, Powden C, O'Neill JP, Sears CL. Serum mannose-binding lectin deficiency is associated with cryptosporidiosis in young Haitian children. Clin Infect Dis. 2006 Aug 1; 43(3):289-94.
  59. Matuskova J, Chauhan AK, Cambien B, Astrof S, Dole VS, Piffath CL, Hynes RO, Wagner DD. Decreased plasma fibronectin leads to delayed thrombus growth in injured arterioles. Arterioscler Thromb Vasc Biol. 2006 Jun; 26(6):1391-6.
  60. Kamath AF, Chauhan AK, Kisucka J, Dole VS, Loscalzo J, Handy DE, Wagner DD. Elevated levels of homocysteine compromise blood-brain barrier integrity in mice. Blood. 2006 Jan 15; 107(2):591-3.
  61. Polgar J, Matuskova J, Wagner DD. The P-selectin, tissue factor, coagulation triad. J Thromb Haemost. 2005 Aug; 3(8):1590-6.
  62. Dole VS, Bergmeier W, Mitchell HA, Eichenberger SC, Wagner DD. Activated platelets induce Weibel-Palade-body secretion and leukocyte rolling in vivo: role of P-selectin. Blood. 2005 Oct 1; 106(7):2334-9.
  63. Cai S, Dole VS, Bergmeier W, Scafidi J, Feng H, Wagner DD, Davis AE. A direct role for C1 inhibitor in regulation of leukocyte adhesion. J Immunol. 2005 May 15; 174(10):6462-6.
  64. Wagner DD. New links between inflammation and thrombosis. Arterioscler Thromb Vasc Biol. 2005 Jul; 25(7):1321-4.
  65. Wang HW, Babic AM, Mitchell HA, Liu K, Wagner DD. Elevated soluble ICAM-1 levels induce immune deficiency and increase adiposity in mice. FASEB J. 2005 Jun; 19(8):1018-20.
  66. Crittenden JR, Bergmeier W, Zhang Y, Piffath CL, Liang Y, Wagner DD, Housman DE, Graybiel AM. CalDAG-GEFI integrates signaling for platelet aggregation and thrombus formation. Nat Med. 2004 Sep; 10(9):982-6.
  67. Babic AM, Wang HW, Lai MJ, Daniels TG, Felbinger TW, Burger PC, Stricker-Krongrad A, Wagner DD. ICAM-1 and beta2 integrin deficiency impairs fat oxidation and insulin metabolism during fasting. Mol Med. 2004 Jul-Dec; 10(7-12):72-9.
  68. Bergmeier W, Rabie T, Strehl A, Piffath CL, Prostredna M, Wagner DD, Nieswandt B. GPVI down-regulation in murine platelets through metalloproteinase-dependent shedding. Thromb Haemost. 2004 May; 91(5):951-8.
  69. Kondo T, Hafezi-Moghadam A, Thomas K, Wagner DD, Kahn CR. Mice lacking insulin or insulin-like growth factor 1 receptors in vascular endothelial cells maintain normal blood-brain barrier. Biochem Biophys Res Commun. 2004 Apr 30; 317(2):315-20.
  70. Cambien B, Wagner DD. A new role in hemostasis for the adhesion receptor P-selectin. Trends Mol Med. 2004 Apr; 10(4):179-86.
  71. Cambien B, Bergmeier W, Saffaripour S, Mitchell HA, Wagner DD. Antithrombotic activity of TNF-alpha. J Clin Invest. 2003 Nov; 112(10):1589-96.
  72. Myers DD, Hawley AE, Farris DM, Wrobleski SK, Thanaporn P, Schaub RG, Wagner DD, Kumar A, Wakefield TW. P-selectin and leukocyte microparticles are associated with venous thrombogenesis. J Vasc Surg. 2003 Nov; 38(5):1075-89.
  73. Wagner DD, Burger PC. Platelets in inflammation and thrombosis. Arterioscler Thromb Vasc Biol. 2003 Dec; 23(12):2131-7.
  74. Bergmeier W, Burger PC, Piffath CL, Hoffmeister KM, Hartwig JH, Nieswandt B, Wagner DD. Metalloproteinase inhibitors improve the recovery and hemostatic function of in vitro-aged or -injured mouse platelets. Blood. 2003 Dec 1; 102(12):4229-35.
  75. Hrachovinová I, Cambien B, Hafezi-Moghadam A, Kappelmayer J, Camphausen RT, Widom A, Xia L, Kazazian HH, Schaub RG, McEver RP, Wagner DD. Interaction of P-selectin and PSGL-1 generates microparticles that correct hemostasis in a mouse model of hemophilia A. Nat Med. 2003 Aug; 9(8):1020-5.
  76. Ni H, Papalia JM, Degen JL, Wagner DD. Control of thrombus embolization and fibronectin internalization by integrin alpha IIb beta 3 engagement of the fibrinogen gamma chain. Blood. 2003 Nov 15; 102(10):3609-14.
  77. Ni H, Yuen PS, Papalia JM, Trevithick JE, Sakai T, Fässler R, Hynes RO, Wagner DD. Plasma fibronectin promotes thrombus growth and stability in injured arterioles. Proc Natl Acad Sci U S A. 2003 Mar 4; 100(5):2415-9.
  78. Italiano JE, Bergmeier W, Tiwari S, Falet H, Hartwig JH, Hoffmeister KM, André P, Wagner DD, Shivdasani RA. Mechanisms and implications of platelet discoid shape. Blood. 2003 Jun 15; 101(12):4789-96.
  79. Hoffmeister KM, Felbinger TW, Falet H, Denis CV, Bergmeier W, Mayadas TN, von Andrian UH, Wagner DD, Stossel TP, Hartwig JH. The clearance mechanism of chilled blood platelets. Cell. 2003 Jan 10; 112(1):87-97.
  80. Burger PC, Wagner DD. Platelet P-selectin facilitates atherosclerotic lesion development. Blood. 2003 Apr 1; 101(7):2661-6.
  81. Sethi S, Ziouzenkova O, Ni H, Wagner DD, Plutzky J, Mayadas TN. Oxidized omega-3 fatty acids in fish oil inhibit leukocyte-endothelial interactions through activation of PPAR alpha. Blood. 2002 Aug 15; 100(4):1340-6.
  82. Götte M, Joussen AM, Klein C, Andre P, Wagner DD, Hinkes MT, Kirchhof B, Adamis AP, Bernfield M. Role of syndecan-1 in leukocyte-endothelial interactions in the ocular vasculature. Invest Ophthalmol Vis Sci. 2002 Apr; 43(4):1135-41.
  83. André P, Prasad KS, Denis CV, He M, Papalia JM, Hynes RO, Phillips DR, Wagner DD. CD40L stabilizes arterial thrombi by a beta3 integrin--dependent mechanism. Nat Med. 2002 Mar; 8(3):247-52.
  84. Methia N, André P, Hafezi-Moghadam A, Economopoulos M, Thomas KL, Wagner DD. ApoE deficiency compromises the blood brain barrier especially after injury. Mol Med. 2001 Dec; 7(12):810-5.
  85. White DG, Zhao S, Sudler R, Ayers S, Friedman S, Chen S, McDermott PF, McDermott S, Wagner DD, Meng J. The isolation of antibiotic-resistant salmonella from retail ground meats. N Engl J Med. 2001 Oct 18; 345(16):1147-54.
  86. Methia N, André P, Denis CV, Economopoulos M, Wagner DD. Localized reduction of atherosclerosis in von Willebrand factor-deficient mice. Blood. 2001 Sep 1; 98(5):1424-8.
  87. Ni H, Ramakrishnan V, Ruggeri ZM, Papalia JM, Phillips DR, Wagner DD. Increased thrombogenesis and embolus formation in mice lacking glycoprotein V. Blood. 2001 Jul 15; 98(2):368-73.
  88. Niederman R, Westernoff T, Lee C, Mark LL, Kawashima N, Ullman-Culler M, Dewhirst FE, Paster BJ, Wagner DD, Mayadas T, Hynes RO, Stashenko P. Infection-mediated early-onset periodontal disease in P/E-selectin-deficient mice. J Clin Periodontol. 2001 Jun; 28(6):569-75.
  89. Denis CV, André P, Saffaripour S, Wagner DD. Defect in regulated secretion of P-selectin affects leukocyte recruitment in von Willebrand factor-deficient mice. Proc Natl Acad Sci U S A. 2001 Mar 27; 98(7):4072-7.
  90. Denis CV, Kwack K, Saffaripour S, Maganti S, André P, Schaub RG, Wagner DD. Interleukin 11 significantly increases plasma von Willebrand factor and factor VIII in wild type and von Willebrand disease mouse models. Blood. 2001 Jan 15; 97(2):465-72.
  91. André P, Hartwell D, Hrachovinová I, Saffaripour S, Wagner DD. Pro-coagulant state resulting from high levels of soluble P-selectin in blood. Proc Natl Acad Sci U S A. 2000 Dec 5; 97(25):13835-40.
  92. Shiromani PJ, Basheer R, Thakkar J, Wagner D, Greco MA, Charness ME. Sleep and wakefulness in c-fos and fos B gene knockout mice. Brain Res Mol Brain Res. 2000 Aug 14; 80(1):75-87.
  93. Ni H, Denis CV, Subbarao S, Degen JL, Sato TN, Hynes RO, Wagner DD. Persistence of platelet thrombus formation in arterioles of mice lacking both von Willebrand factor and fibrinogen. J Clin Invest. 2000 Aug; 106(3):385-92.
  94. Dong ZM, Brown AA, Wagner DD. Prominent role of P-selectin in the development of advanced atherosclerosis in ApoE-deficient mice. Circulation. 2000 May 16; 101(19):2290-5.
  95. Frenette PS, Denis CV, Weiss L, Jurk K, Subbarao S, Kehrel B, Hartwig JH, Vestweber D, Wagner DD. P-Selectin glycoprotein ligand 1 (PSGL-1) is expressed on platelets and can mediate platelet-endothelial interactions in vivo. J Exp Med. 2000 Apr 17; 191(8):1413-22.
  96. Wagner D, Salin-Pascual R, Greco MA, Shiromani PJ. Distribution of hypocretin-containing neurons in the lateral hypothalamus and C-fos-immunoreactive neurons in the VLPO. Sleep Res Online. 2000; 3(1):35-42.
  97. Methia N, Denis CV, Wagner DD. Carboxypeptidase E does not mediate von Willebrand factor targeting to storage granules. Eur J Cell Biol. 1999 Dec; 78(12):884-91.
  98. Denis CV, Wagner DD. Insights from von Willebrand disease animal models. Cell Mol Life Sci. 1999 Dec; 56(11-12):977-90.
  99. Robinson SD, Frenette PS, Rayburn H, Cummiskey M, Ullman-Culleré M, Wagner DD, Hynes RO. Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A. 1999 Sep 28; 96(20):11452-7.
  100. Enjyoji K, Sévigny J, Lin Y, Frenette PS, Christie PD, Esch JS, Imai M, Edelberg JM, Rayburn H, Lech M, Beeler DL, Csizmadia E, Wagner DD, Robson SC, Rosenberg RD. Targeted disruption of cd39/ATP diphosphohydrolase results in disordered hemostasis and thromboregulation. Nat Med. 1999 Sep; 5(9):1010-7.
  101. Hartwell DW, Wagner DD. New discoveries with mice mutant in endothelial and platelet selectins. Thromb Haemost. 1999 Aug; 82(2):850-7.
  102. Soriano SG, Wang YF, Wagner DD, Frenette PS. P- and E-selectin-deficient mice are susceptible to cerebral ischemia-reperfusion injury. Brain Res. 1999 Jul 24; 835(2):360-4.
  103. Shi C, Feinberg MW, Zhang D, Patel A, Sim CU, Dong ZM, Chapman SM, Gutierrez-Ramos JC, Wagner DD, Sibinga NE, Haber E. Donor MHC and adhesion molecules in transplant arteriosclerosis. J Clin Invest. 1999 Feb; 103(4):469-74.
  104. Jia GQ, Gonzalo JA, Hidalgo A, Wagner D, Cybulsky M, Gutierrez-Ramos JC. Selective eosinophil transendothelial migration triggered by eotaxin via modulation of Mac-1/ICAM-1 and VLA-4/VCAM-1 interactions. Int Immunol. 1999 Jan; 11(1):1-10.
  105. Berger G, Hartwell DW, Wagner DD. P-Selectin and platelet clearance. Blood. 1998 Dec 1; 92(11):4446-52.
  106. Frenette PS, Subbarao S, Mazo IB, von Andrian UH, Wagner DD. Endothelial selectins and vascular cell adhesion molecule-1 promote hematopoietic progenitor homing to bone marrow. Proc Natl Acad Sci U S A. 1998 Nov 24; 95(24):14423-8.
  107. Hartwell DW, Mayadas TN, Berger G, Frenette PS, Rayburn H, Hynes RO, Wagner DD. Role of P-selectin cytoplasmic domain in granular targeting in vivo and in early inflammatory responses. J Cell Biol. 1998 Nov 16; 143(4):1129-41.
  108. Dong ZM, Wagner DD. Leukocyte-endothelium adhesion molecules in atherosclerosis. J Lab Clin Med. 1998 Nov; 132(5):369-75.
  109. Homeister JW, Zhang M, Frenette PS, Hynes RO, Wagner DD, Lowe JB, Marks RM. Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation. Blood. 1998 Oct 1; 92(7):2345-52.
  110. Seiler KP, Ma Y, Weis JH, Frenette PS, Hynes RO, Wagner DD, Weis JJ. E and P selectins are not required for resistance to severe murine lyme arthritis. Infect Immun. 1998 Sep; 66(9):4557-9.
  111. Denis C, Methia N, Frenette PS, Rayburn H, Ullman-Culleré M, Hynes RO, Wagner DD. A mouse model of severe von Willebrand disease: defects in hemostasis and thrombosis. Proc Natl Acad Sci U S A. 1998 Aug 4; 95(16):9524-9.
  112. Vogt PM, Lehnhardt M, Wagner D, Jansen V, Krieg M, Steinau HU. Determination of endogenous growth factors in human wound fluid: temporal presence and profiles of secretion. Plast Reconstr Surg. 1998 Jul; 102(1):117-23.
  113. Dong ZM, Chapman SM, Brown AA, Frenette PS, Hynes RO, Wagner DD. The combined role of P- and E-selectins in atherosclerosis. J Clin Invest. 1998 Jul 1; 102(1):145-52.
  114. Fleming JC, Berger G, Guichard J, Cramer EM, Wagner DD. The transmembrane domain enhances granular targeting of P-selectin. Eur J Cell Biol. 1998 Apr; 75(4):331-43.
  115. Vogt PM, Lehnhardt M, Wagner D, Steinau HU. Growth factors and insulin-like growth factor binding proteins in acute wound fluid. Growth Horm IGF Res. 1998 Apr; 8 Suppl B:107-9.
  116. de Mora F, Williams CM, Frenette PS, Wagner DD, Hynes RO, Galli SJ. P- and E-selectins are required for the leukocyte recruitment, but not the tissue swelling, associated with IgE- and mast cell-dependent inflammation in mouse skin. Lab Invest. 1998 Apr; 78(4):497-505.
  117. Frenette PS, Moyna C, Hartwell DW, Lowe JB, Hynes RO, Wagner DD. Platelet-endothelial interactions in inflamed mesenteric venules. Blood. 1998 Feb 15; 91(4):1318-24.
  118. Hartwell DW, Butterfield CE, Frenette PS, Kenyon BM, Hynes RO, Folkman J, Wagner DD. Angiogenesis in P- and E-selectin-deficient mice. Microcirculation. 1998; 5(2-3):173-8.
  119. Dong ZM, Wagner DD. Leukocytes fighting against obesity. Mol Psychiatry. 1998 Jan; 3(1):8-9.
  120. Hynes RO, Wagner DD. Genetic manipulation of vascular adhesion molecules in mice. J Clin Invest. 1997 Dec 1; 100(11 Suppl):S11-3.
  121. Weiser MR, Pechet TT, Williams JP, Ma M, Frenette PS, Moore FD, Kobzik L, Hines RO, Wagner DD, Carroll MC, Hechtman HB. Experimental murine acid aspiration injury is mediated by neutrophils and the alternative complement pathway. J Appl Physiol (1985). 1997 Oct; 83(4):1090-5.
  122. Walter UM, Ayer LM, Manning AM, Frenette PS, Wagner DD, Hynes RO, Wolitzky BA, Issekutz AC. Generation and characterization of a novel adhesion function blocking monoclonal antibody recognizing both rat and mouse E-selectin. Hybridoma. 1997 Aug; 16(4):355-61.
  123. Dong ZM, Gutierrez-Ramos JC, Coxon A, Mayadas TN, Wagner DD. A new class of obesity genes encodes leukocyte adhesion receptors. Proc Natl Acad Sci U S A. 1997 Jul 8; 94(14):7526-30.
  124. Frenette PS, Wagner DD. Insights into selectin function from knockout mice. Thromb Haemost. 1997 Jul; 78(1):60-4.
  125. Walter UM, Ayer LM, Wolitzky BA, Wagner DD, Hynes RO, Manning AM, Issekutz AC. Characterization of a novel adhesion function blocking monoclonal antibody to rat/mouse P-selectin generated in the P-selectin-deficient mouse. Hybridoma. 1997 Jun; 16(3):249-57.
  126. Subramaniam M, Saffaripour S, Van De Water L, Frenette PS, Mayadas TN, Hynes RO, Wagner DD. Role of endothelial selectins in wound repair. Am J Pathol. 1997 May; 150(5):1701-9.
  127. Burke PA, Stack JA, Wagner D, Lewis DW, Jenkins RL, Forse RA. L-[1-(13)C] Phenylalanine oxidation as a measure of hepatocyte functional capacity in end-stage liver disease. Am J Surg. 1997 Apr; 173(4):270-3; discussion 273-4.
  128. Johnson RC, Chapman SM, Dong ZM, Ordovas JM, Mayadas TN, Herz J, Hynes RO, Schaefer EJ, Wagner DD. Absence of P-selectin delays fatty streak formation in mice. J Clin Invest. 1997 Mar 1; 99(5):1037-43.
  129. Xiang M, Gan L, Li D, Zhou L, Chen ZY, Wagner D, O'Malley BW, Klein W, Nathans J. Role of the Brn-3 family of POU-domain genes in the development of the auditory/vestibular, somatosensory, and visual systems. Cold Spring Harb Symp Quant Biol. 1997; 62:325-36.
  130. Hynes RO, Wagner DD. Genetic manipulation of vascular adhesion molecules in mice. J Clin Invest. 1996 Nov 15; 98(10):2193-5.
  131. Kolbinger W, Wagner D, Wagner HJ. Control of rod retinomotor movements in teleost retinae: the role of dopamine in mediating light-dependent and circadian signals. Cell Tissue Res. 1996 Sep; 285(3):445-51.
  132. Frenette PS, Wagner DD. Adhesion molecules--Part II: Blood vessels and blood cells. N Engl J Med. 1996 Jul 4; 335(1):43-5.
  133. Frenette PS, Wagner DD. Adhesion molecules--Part 1. N Engl J Med. 1996 Jun 6; 334(23):1526-9.
  134. Tang T, Frenette PS, Hynes RO, Wagner DD, Mayadas TN. Cytokine-induced meningitis is dramatically attenuated in mice deficient in endothelial selectins. J Clin Invest. 1996 Jun 1; 97(11):2485-90.
  135. Frenette PS, Mayadas TN, Rayburn H, Hynes RO, Wagner DD. Double knockout highlights value of endothelial selectins. Immunol Today. 1996 May; 17(5):205.
  136. Mayadas TN, Mendrick DL, Brady HR, Tang T, Papayianni A, Assmann KJ, Wagner DD, Hynes RO, Cotran RS. Acute passive anti-glomerular basement membrane nephritis in P-selectin-deficient mice. Kidney Int. 1996 May; 49(5):1342-9.
  137. Frenette PS, Mayadas TN, Rayburn H, Hynes RO, Wagner DD. Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell. 1996 Feb 23; 84(4):563-74.
  138. Subramaniam M, Frenette PS, Saffaripour S, Johnson RC, Hynes RO, Wagner DD. Defects in hemostasis in P-selectin-deficient mice. Blood. 1996 Feb 15; 87(4):1238-42.
  139. Yamada S, Mayadas TN, Yuan F, Wagner DD, Hynes RO, Melder RJ, Jain RK. Rolling in P-selectin-deficient mice is reduced but not eliminated in the dorsal skin. Blood. 1995 Nov 1; 86(9):3487-92.
  140. Johnson RC, Mayadas TN, Frenette PS, Mebius RE, Subramaniam M, Lacasce A, Hynes RO, Wagner DD. Blood cell dynamics in P-selectin-deficient mice. Blood. 1995 Aug 1; 86(3):1106-14.
  141. Frenette PS, Johnson RC, Hynes RO, Wagner DD. Platelets roll on stimulated endothelium in vivo: an interaction mediated by endothelial P-selectin. Proc Natl Acad Sci U S A. 1995 Aug 1; 92(16):7450-4.
  142. Subramaniam M, Saffaripour S, Watson SR, Mayadas TN, Hynes RO, Wagner DD. Reduced recruitment of inflammatory cells in a contact hypersensitivity response in P-selectin-deficient mice. J Exp Med. 1995 Jun 1; 181(6):2277-82.
  143. Wagner DD. P-selectin chases a butterfly. J Clin Invest. 1995 May; 95(5):1955-6.
  144. Wagner DD. P-selectin knockout: a mouse model for various human diseases. Ciba Found Symp. 1995; 189:2-10; discussion 10-16, 77-8.
  145. Vischer UM, Wagner DD. von Willebrand factor proteolytic processing and multimerization precede the formation of Weibel-Palade bodies. Blood. 1994 Jun 15; 83(12):3536-44.
  146. Journet AM, Saffaripour S, Wagner DD. Requirement for both D domains of the propolypeptide in von Willebrand factor multimerization and storage. Thromb Haemost. 1993 Dec 20; 70(6):1053-7.
  147. Vischer UM, Wagner DD. CD63 is a component of Weibel-Palade bodies of human endothelial cells. Blood. 1993 Aug 15; 82(4):1184-91.
  148. Mayadas TN, Johnson RC, Rayburn H, Hynes RO, Wagner DD. Leukocyte rolling and extravasation are severely compromised in P selectin-deficient mice. Cell. 1993 Aug 13; 74(3):541-54.
  149. Stone JP, Wagner DD. P-selectin mediates adhesion of platelets to neuroblastoma and small cell lung cancer. J Clin Invest. 1993 Aug; 92(2):804-13.
  150. Subramaniam M, Koedam JA, Wagner DD. Divergent fates of P- and E-selectins after their expression on the plasma membrane. Mol Biol Cell. 1993 Aug; 4(8):791-801.
  151. Wagner DD. The Weibel-Palade body: the storage granule for von Willebrand factor and P-selectin. Thromb Haemost. 1993 Jul 1; 70(1):105-10.
  152. Journet AM, Saffaripour S, Cramer EM, Tenza D, Wagner DD. von Willebrand factor storage requires intact prosequence cleavage site. Eur J Cell Biol. 1993 Feb; 60(1):31-41.
  153. Deng XW, Matsui M, Wei N, Wagner D, Chu AM, Feldmann KA, Quail PH. COP1, an Arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a G beta homologous domain. Cell. 1992 Nov 27; 71(5):791-801.
  154. Larsen GR, Sako D, Ahern TJ, Shaffer M, Erban J, Sajer SA, Gibson RM, Wagner DD, Furie BC, Furie B. P-selectin and E-selectin. Distinct but overlapping leukocyte ligand specificities. J Biol Chem. 1992 Jun 5; 267(16):11104-10.
  155. Mayadas TN, Wagner DD. Vicinal cysteines in the prosequence play a role in von Willebrand factor multimer assembly. Proc Natl Acad Sci U S A. 1992 Apr 15; 89(8):3531-5.
  156. Erban JK, Wagner DD. A 130-kDa protein on endothelial cells binds to amino acids 15-42 of the B beta chain of fibrinogen. J Biol Chem. 1992 Feb 5; 267(4):2451-8.
  157. Koedam JA, Cramer EM, Briend E, Furie B, Furie BC, Wagner DD. P-selectin, a granule membrane protein of platelets and endothelial cells, follows the regulated secretory pathway in AtT-20 cells. J Cell Biol. 1992 Feb; 116(3):617-25.
  158. Wagner DD, Bonfanti R. von Willebrand factor and the endothelium. Mayo Clin Proc. 1991 Jun; 66(6):621-7.
  159. Wagner DD, Saffaripour S, Bonfanti R, Sadler JE, Cramer EM, Chapman B, Mayadas TN. Induction of specific storage organelles by von Willebrand factor propolypeptide. Cell. 1991 Jan 25; 64(2):403-13.
  160. Mayadas TN, Wagner DD. von Willebrand factor biosynthesis and processing. Ann N Y Acad Sci. 1991; 614:153-66.
  161. Furie B, Celi A, Palabrica TM, Larsen E, Wagner DD, Furie BC. PADGEM, a leukocyte receptor on activated platelets. Biology and application to in vivo medical diagnostics. Curr Stud Hematol Blood Transfus. 1991; (58):32-6.
  162. Larsen E, Palabrica T, Sajer S, Gilbert GE, Wagner DD, Furie BC, Furie B. PADGEM-dependent adhesion of platelets to monocytes and neutrophils is mediated by a lineage-specific carbohydrate, LNF III (CD15). Cell. 1990 Nov 2; 63(3):467-74.
  163. Peters JH, Sporn LA, Ginsberg MH, Wagner DD. Human endothelial cells synthesize, process, and secrete fibronectin molecules bearing an alternatively spliced type III homology (ED1). Blood. 1990 May 1; 75(9):1801-8.
  164. Wagner DD. Cell biology of von Willebrand factor. Annu Rev Cell Biol. 1990; 6:217-46.
  165. Larsen E, Celi A, Gilbert GE, Furie BC, Erban JK, Bonfanti R, Wagner DD, Furie B. PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes. Cell. 1989 Oct 20; 59(2):305-12.
  166. Mayadas TN, Wagner DD. In vitro multimerization of von Willebrand factor is triggered by low pH. Importance of the propolypeptide and free sulfhydryls. J Biol Chem. 1989 Aug 15; 264(23):13497-503.
  167. Ribes JA, Ni F, Wagner DD, Francis CW. Mediation of fibrin-induced release of von Willebrand factor from cultured endothelial cells by the fibrin beta chain. J Clin Invest. 1989 Aug; 84(2):435-42.
  168. Roarke MC, Wagner DD, Marder VJ, Sporn LA. Temperature-sensitive steps in the secretory pathway for von Willebrand factor in endothelial cells. Eur J Cell Biol. 1989 Apr; 48(2):337-43.
  169. Sporn LA, Marder VJ, Wagner DD. Differing polarity of the constitutive and regulated secretory pathways for von Willebrand factor in endothelial cells. J Cell Biol. 1989 Apr; 108(4):1283-9.
  170. Bonfanti R, Furie BC, Furie B, Wagner DD. PADGEM (GMP140) is a component of Weibel-Palade bodies of human endothelial cells. Blood. 1989 Apr; 73(5):1109-12.
  171. Mayadas T, Wagner DD, Simpson PJ. von Willebrand factor biosynthesis and partitioning between constitutive and regulated pathways of secretion after thrombin stimulation. Blood. 1989 Feb 15; 73(3):706-11.
  172. Handin RI, Wagner DD. Molecular and cellular biology of von Willebrand factor. Prog Hemost Thromb. 1989; 9:233-59.
  173. Sinha S, Wagner DD. Intact microtubules are necessary for complete processing, storage and regulated secretion of von Willebrand factor by endothelial cells. Eur J Cell Biol. 1987 Jun; 43(3):377-83.
  174. Sporn LA, Marder VJ, Wagner DD. von Willebrand factor released from Weibel-Palade bodies binds more avidly to extracellular matrix than that secreted constitutively. Blood. 1987 May; 69(5):1531-4.
  175. Wagner DD, Fay PJ, Sporn LA, Sinha S, Lawrence SO, Marder VJ. Divergent fates of von Willebrand factor and its propolypeptide (von Willebrand antigen II) after secretion from endothelial cells. Proc Natl Acad Sci U S A. 1987 Apr; 84(7):1955-9.
  176. Ribes JA, Francis CW, Wagner DD. Fibrin induces release of von Willebrand factor from endothelial cells. J Clin Invest. 1987 Jan; 79(1):117-23.
  177. Wagner DD, Lawrence SO, Ohlsson-Wilhelm BM, Fay PJ, Marder VJ. Topology and order of formation of interchain disulfide bonds in von Willebrand factor. Blood. 1987 Jan; 69(1):27-32.
  178. Sporn LA, Marder VJ, Wagner DD. Inducible secretion of large, biologically potent von Willebrand factor multimers. Cell. 1986 Jul 18; 46(2):185-90.
  179. Fay PJ, Kawai Y, Wagner DD, Ginsburg D, Bonthron D, Ohlsson-Wilhelm BM, Chavin SI, Abraham GN, Handin RI, Orkin SH, et al. Propolypeptide of von Willebrand factor circulates in blood and is identical to von Willebrand antigen II. Science. 1986 May 23; 232(4753):995-8.
  180. Wagner DD, Mayadas T, Marder VJ. Initial glycosylation and acidic pH in the Golgi apparatus are required for multimerization of von Willebrand factor. J Cell Biol. 1986 Apr; 102(4):1320-4.
  181. Sporn LA, Chavin SI, Marder VJ, Wagner DD. Biosynthesis of von Willebrand protein by human megakaryocytes. J Clin Invest. 1985 Sep; 76(3):1102-6.
  182. Wagner DD, Mayadas T, Urban-Pickering M, Lewis BH, Marder VJ. Inhibition of disulfide bonding of von Willebrand protein by monensin results in small, functionally defective multimers. J Cell Biol. 1985 Jul; 101(1):112-20.
  183. Wagner DD, Marder VJ. Biosynthesis of von Willebrand protein by human endothelial cells: processing steps and their intracellular localization. J Cell Biol. 1984 Dec; 99(6):2123-30.
  184. Sporn LA, Rubin P, Marder VJ, Wagner DD. Irradiation induces release of von Willebrand protein from endothelial cells in culture. Blood. 1984 Aug; 64(2):567-70.
  185. Wagner DD, Urban-Pickering M, Marder VJ. Von Willebrand protein binds to extracellular matrices independently of collagen. Proc Natl Acad Sci U S A. 1984 Jan; 81(2):471-5.
  186. Wagner DD, Marder VJ. Biosynthesis of von Willebrand protein by human endothelial cells. Identification of a large precursor polypeptide chain. J Biol Chem. 1983 Feb 25; 258(4):2065-7.
  187. Wagner DD, Olmsted JB, Marder VJ. Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells. J Cell Biol. 1982 Oct; 95(1):355-60.
  188. Wagner DD, Hynes RO. Fibronectin-coated beads are endocytosed by cells and align with microfilament bundles. Exp Cell Res. 1982 Aug; 140(2):373-81.
  189. Hynes RO, Destree AT, Wagner DD. Relationships between microfilaments, cell-substratum adhesion, and fibronectin. Cold Spring Harb Symp Quant Biol. 1982; 46 Pt 2:659-70.
  190. Wagner DD, Ivatt R, Destree AT, Hynes RO. Similarities and differences between the fibronectins of normal and transformed hamster cells. J Biol Chem. 1981 Nov 25; 256(22):11708-15.
  191. Wagner DD, Hynes RO. Topological arrangement of the major structural features of fibronectin. J Biol Chem. 1980 May 10; 255(9):4304-12.
  192. Wagner DD, Hynes RO. Domain structure of fibronectin and its relation to function. Disulfides and sulfhydryl groups. J Biol Chem. 1979 Jul 25; 254(14):6746-54.
  193. Hynes RO, Destree AT, Perkins ME, Wagner DD. Cell surface fibronectin and oncogenic transformation. J Supramol Struct. 1979; 11(1):95-104.
  194. Hynes RO, Ali IU, Destree AT, Mautner V, Perkins ME, Senger DR, Wagner DD, Smith KK. A large glycoprotein lost from the surfaces of transformed cells. Ann N Y Acad Sci. 1978 Jun 20; 312:317-42.
  195. Canault M, Certel K, Schatzberg D, Wagner DD, Hynes RO. The lack of ADAM17 activity during embryonic development causes hemorrhage and impairs vessel formation. PLoS One. 2010 Oct 15; 5(10):e13433.
  196. Hirahashi J, Mekala D, Van Ziffle J, Xiao L, Saffaripour S, Wagner DD, Shapiro SD, Lowell C, Mayadas TN. Mac-1 signaling via Src-family and Syk kinases results in elastase-dependent thrombohemorrhagic vasculopathy. Immunity. 2006 Aug; 25(2):271-83.
  197. Matsushita K, Morrell CN, Cambien B, Yang SX, Yamakuchi M, Bao C, Hara MR, Quick RA, Cao W, O'Rourke B, Lowenstein JM, Pevsner J, Wagner DD, Lowenstein CJ. Nitric oxide regulates exocytosis by S-nitrosylation of N-ethylmaleimide-sensitive factor. Cell. 2003 Oct 17; 115(2):139-50.
  198. Zhu L, Bergmeier W, Wu J, Jiang H, Stalker TJ, Cieslak M, Fan R, Boumsell L, Kumanogoh A, Kikutani H, Tamagnone L, Wagner DD, Milla ME, Brass LF. Regulated surface expression and shedding support a dual role for semaphorin 4D in platelet responses to vascular injury. Proc Natl Acad Sci U S A. 2007 Jan 30; 104(5):1621-6.
  199. De Meyer SF, Savchenko AS, Haas MS, Schatzberg D, Carroll MC, Schiviz A, Dietrich B, Rottensteiner H, Scheiflinger F, Wagner DD. Protective anti-inflammatory effect of ADAMTS13 on myocardial ischemia/reperfusion injury in mice. Blood. 2012 Dec 20; 120(26):5217-23.
  200. Falet H, Pollitt AY, Begonja AJ, Weber SE, Duerschmied D, Wagner DD, Watson SP, Hartwig JH. A novel interaction between FlnA and Syk regulates platelet ITAM-mediated receptor signaling and function. J Exp Med. 2010 Aug 30; 207(9):1967-79.
  201. Chauhan AK, Walsh MT, Zhu G, Ginsburg D, Wagner DD, Motto DG. The combined roles of ADAMTS13 and VWF in murine models of TTP, endotoxemia, and thrombosis. Blood. 2008 Apr 1; 111(7):3452-7.
  202. Demers M, Ho-Tin-Noé B, Schatzberg D, Yang JJ, Wagner DD. Increased efficacy of breast cancer chemotherapy in thrombocytopenic mice. Cancer Res. 2011 Mar 1; 71(5):1540-9.
  203. De Meyer SF, Suidan GL, Fuchs TA, Monestier M, Wagner DD. Extracellular chromatin is an important mediator of ischemic stroke in mice. Arterioscler Thromb Vasc Biol. 2012 Aug; 32(8):1884-91.
  204. Bergmeier W, Chauhan AK, Wagner DD. Glycoprotein Ibalpha and von Willebrand factor in primary platelet adhesion and thrombus formation: lessons from mutant mice. Thromb Haemost. 2008 Feb; 99(2):264-70.
  205. Kisucka J, Butterfield CE, Duda DG, Eichenberger SC, Saffaripour S, Ware J, Ruggeri ZM, Jain RK, Folkman J, Wagner DD. Platelets and platelet adhesion support angiogenesis while preventing excessive hemorrhage. Proc Natl Acad Sci U S A. 2006 Jan 24; 103(4):855-60.
  206. Chauhan AK, Motto DG, Lamb CB, Bergmeier W, Dockal M, Plaimauer B, Scheiflinger F, Ginsburg D, Wagner DD. Systemic antithrombotic effects of ADAMTS13. J Exp Med. 2006 Mar 20; 203(3):767-76.
  207. Motto DG, Chauhan AK, Zhu G, Homeister J, Lamb CB, Desch KC, Zhang W, Tsai HM, Wagner DD, Ginsburg D. Shigatoxin triggers thrombotic thrombocytopenic purpura in genetically susceptible ADAMTS13-deficient mice. J Clin Invest. 2005 Oct; 115(10):2752-61.
  208. Bergmeier W, Piffath CL, Cheng G, Dole VS, Zhang Y, von Andrian UH, Wagner DD. Tumor necrosis factor-alpha-converting enzyme (ADAM17) mediates GPIbalpha shedding from platelets in vitro and in vivo. Circ Res. 2004 Oct 1; 95(7):677-83.
  209. Vargas Hein O, Staegemann M, Wagner D, von Heymann C, Martin M, Morgera S, Spies C. Torsemide versus furosemide after continuous renal replacement therapy due to acute renal failure in cardiac surgery patients. Ren Fail. 2005; 27(4):385-92.
  210. Ho-Tin-Noé B, Demers M, Wagner DD. How platelets safeguard vascular integrity. J Thromb Haemost. 2011 Jul; 9 Suppl 1:56-65.
  211. Brill A, Chauhan AK, Canault M, Walsh MT, Bergmeier W, Wagner DD. Oxidative stress activates ADAM17/TACE and induces its target receptor shedding in platelets in a p38-dependent fashion. Cardiovasc Res. 2009 Oct 1; 84(1):137-44.
  212. Wagner D, Hörster R, Lange B, Lange C. [Evaluation of T-cell interferon-gamma-release assays for the diagnosis of latent and active tuberculosis]. Dtsch Med Wochenschr. 2008 Feb; 133(8):354-7.
  213. Canault M, Duerschmied D, Brill A, Stefanini L, Schatzberg D, Cifuni SM, Bergmeier W, Wagner DD. p38 mitogen-activated protein kinase activation during platelet storage: consequences for platelet recovery and hemostatic function in vivo. Blood. 2010 Mar 4; 115(9):1835-42.
  214. Dole VS, Matuskova J, Vasile E, Yesilaltay A, Bergmeier W, Bernimoulin M, Wagner DD, Krieger M. Thrombocytopenia and platelet abnormalities in high-density lipoprotein receptor-deficient mice. Arterioscler Thromb Vasc Biol. 2008 Jun; 28(6):1111-6.
  215. Frederix K, Chauhan AK, Kisucka J, Zhao BQ, Hoff EI, Spronk HM, Ten Cate H, Wagner DD. Platelet adhesion receptors do not modulate infarct volume after a photochemically induced stroke in mice. Brain Res. 2007 Dec 14; 1185:239-45.
  216. Mazo IB, Gutierrez-Ramos JC, Frenette PS, Hynes RO, Wagner DD, von Andrian UH. Hematopoietic progenitor cell rolling in bone marrow microvessels: parallel contributions by endothelial selectins and vascular cell adhesion molecule 1. J Exp Med. 1998 Aug 3; 188(3):465-74.
  217. André P, Denis CV, Ware J, Saffaripour S, Hynes RO, Ruggeri ZM, Wagner DD. Platelets adhere to and translocate on von Willebrand factor presented by endothelium in stimulated veins. Blood. 2000 Nov 15; 96(10):3322-8.
  218. Duerschmied D, Canault M, Lievens D, Brill A, Cifuni SM, Bader M, Wagner DD. Serotonin stimulates platelet receptor shedding by tumor necrosis factor-alpha-converting enzyme (ADAM17). J Thromb Haemost. 2009 Jul; 7(7):1163-71.
  219. De Meyer SF, Stoll G, Wagner DD, Kleinschnitz C. von Willebrand factor: an emerging target in stroke therapy. Stroke. 2012 Feb; 43(2):599-606.
  220. Ho-Tin-Noé B, Goerge T, Cifuni SM, Duerschmied D, Wagner DD. Platelet granule secretion continuously prevents intratumor hemorrhage. Cancer Res. 2008 Aug 15; 68(16):6851-8.
  221. Jansen AJ, Josefsson EC, Rumjantseva V, Liu QP, Falet H, Bergmeier W, Cifuni SM, Sackstein R, von Andrian UH, Wagner DD, Hartwig JH, Hoffmeister KM. Desialylation accelerates platelet clearance after refrigeration and initiates GPIba metalloproteinase-mediated cleavage in mice. Blood. 2012 Feb 2; 119(5):1263-73.
  222. Banno F, Chauhan AK, Kokame K, Yang J, Miyata S, Wagner DD, Miyata T. The distal carboxyl-terminal domains of ADAMTS13 are required for regulation of in vivo thrombus formation. Blood. 2009 May 21; 113(21):5323-9.
  223. Crescente M, Thomas GM, Demers M, Voorhees JR, Wong SL, Ho-Tin-Noé B, Wagner DD. ADAMTS13 exerts a thrombolytic effect in microcirculation. Thromb Haemost. 2012 Sep; 108(3):527-32.
  224. Chauhan AK, Goerge T, Schneider SW, Wagner DD. Formation of platelet strings and microthrombi in the presence of ADAMTS-13 inhibitor does not require P-selectin or beta3 integrin. J Thromb Haemost. 2007 Mar; 5(3):583-9.
  225. Stadtmann A, Brinkhaus L, Mueller H, Rossaint J, Bolomini-Vittori M, Bergmeier W, Van Aken H, Wagner DD, Laudanna C, Ley K, Zarbock A. Rap1a activation by CalDAG-GEFI and p38 MAPK is involved in E-selectin-dependent slow leukocyte rolling. Eur J Immunol. 2011 Jul; 41(7):2074-85.
  226. Aktas B, Pozgajova M, Bergmeier W, Sunnarborg S, Offermanns S, Lee D, Wagner DD, Nieswandt B. Aspirin induces platelet receptor shedding via ADAM17 (TACE). J Biol Chem. 2005 Dec 2; 280(48):39716-22.
  227. Bergmeier W, Piffath CL, Goerge T, Cifuni SM, Ruggeri ZM, Ware J, Wagner DD. The role of platelet adhesion receptor GPIbalpha far exceeds that of its main ligand, von Willebrand factor, in arterial thrombosis. Proc Natl Acad Sci U S A. 2006 Nov 7; 103(45):16900-5.
  228. Walshe TE, Dole VS, Maharaj AS, Patten IS, Wagner DD, D'Amore PA. Inhibition of VEGF or TGF-{beta} signaling activates endothelium and increases leukocyte rolling. Arterioscler Thromb Vasc Biol. 2009 Aug; 29(8):1185-92.
  229. Chauhan AK, Kisucka J, Brill A, Walsh MT, Scheiflinger F, Wagner DD. ADAMTS13: a new link between thrombosis and inflammation. J Exp Med. 2008 Sep 1; 205(9):2065-74.
  230. Zhao BQ, Chauhan AK, Canault M, Patten IS, Yang JJ, Dockal M, Scheiflinger F, Wagner DD. von Willebrand factor-cleaving protease ADAMTS13 reduces ischemic brain injury in experimental stroke. Blood. 2009 Oct 8; 114(15):3329-34.
  231. Pinsky DJ, Naka Y, Liao H, Oz MC, Wagner DD, Mayadas TN, Johnson RC, Hynes RO, Heath M, Lawson CA, Stern DM. Hypoxia-induced exocytosis of endothelial cell Weibel-Palade bodies. A mechanism for rapid neutrophil recruitment after cardiac preservation. J Clin Invest. 1996 Jan 15; 97(2):493-500.
  232. Chauhan AK, Kisucka J, Cozzi MR, Walsh MT, Moretti FA, Battiston M, Mazzucato M, De Marco L, Baralle FE, Wagner DD, Muro AF. Prothrombotic effects of fibronectin isoforms containing the EDA domain. Arterioscler Thromb Vasc Biol. 2008 Feb; 28(2):296-301.
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