Research Overview

Nicholas Stylopoulos is a Principal investigator at the Division of Endocrinology and an Assistant Professor at Harvard Medical School. His laboratory is part of the Center for Basic and Translational Obesity Research and focuses on the mechanisms by which weight loss surgery affects energy balance.

The Center for Basic and Translational Obesity Research is an exciting new research program at the Division of Endocrinology at Children’s Hospital Boston and Harvard Medical School, whose goal is to improve our understanding of how people become obese or suffer from complications of obesity, and to help translate research findings into better treatments. The Director of the Center is Dr. Joel Hirschhorn. Dr. Nada Kalaany is a Principal Investigator at the Center.

The Stylopoulos laboratory uses many gastrointestinal weight loss surgery rodent models to study weight loss procedures (e.g., Roux-en-Y gastric bypass, sleeve gastrectomy, gastric banding and other). These models represent a powerful, novel, translational and clinically relevant model system to study regulation of energy balance and metabolic function. They offer a unique opportunity to unravel mechanisms underlying the beneficial effects of surgery on body weight, metabolism, glucose homeostasis, inflammation and cancer.

By “reverse engineering” the mechanisms by which weight loss surgery induces weight loss and resolution of diabetes and other metabolic complications, we will be able to develop less invasive approaches that will utilize the same effective mechanisms without the invasiveness of surgery. Thus, we will eventually “bypass the bypass” and we will be able to increase the number of treatment options and offer less-invasive options that will allow the widespread use in many categories of patients with obesity, including children and adolescents.

Researcher Services

Researcher Areas

  • Metabolism
  • Diabetes
  • Weight Regulation
  • Novel Therapeutics for Metabolic Diseases

Research Departments

Research Divisions

Researcher Labs

PUBLICATIONS

Publications powered by Harvard Catalyst Profiles

  1. Tao R, Wang C, Stöhr O, Qiu W, Hu Y, Miao J, Dong XC, Leng S, Stefater M, Stylopoulos N, Lin L, Copps KD, White MF. Inactivating hepatic follistatin alleviates hyperglycemia. Nat Med. 2018 Jul; 24(7):1058-1069. View abstract
  2. Meoli L, Gupta NK, Saeidi N, Panciotti C, Biddinger SB, Corey KE, Stylopoulos N. Non-alcoholic Fatty Liver Disease and Gastric Bypass Surgery Regulate Serum And Hepatic Levels of Pyruvate Kinase Isoenzyme M2. Am J Physiol Endocrinol Metab. 2018 Feb 20. View abstract
  3. Zaytouni T, Tsai PY, Hitchcock DS, DuBois CD, Freinkman E, Lin L, Morales-Oyarvide V, Lenehan PJ, Wolpin BM, Mino-Kenudson M, Torres EM, Stylopoulos N, Clish CB, Kalaany NY. Critical role for arginase 2 in obesity-associated pancreatic cancer. Nat Commun. 2017 08 14; 8(1):242. View abstract
  4. Mumphrey MB, Hao Z, Townsend RL, Patterson LM, Morrison CD, Münzberg H, Stylopoulos N, Ye J, Berthoud HR. Reversible hyperphagia and obesity in rats with gastric bypass by central MC3/4R blockade. Obesity (Silver Spring). 2014 Aug; 22(8):1847-53. View abstract
  5. Ye J, Hao Z, Mumphrey MB, Townsend RL, Patterson LM, Stylopoulos N, Münzberg H, Morrison CD, Drucker DJ, Berthoud HR. GLP-1 receptor signaling is not required for reduced body weight after RYGB in rodents. Am J Physiol Regul Integr Comp Physiol. 2014 Mar 01; 306(5):R352-62. View abstract
  6. Saeidi N, Meoli L, Nestoridi E, Gupta NK, Kvas S, Kucharczyk J, Bonab AA, Fischman AJ, Yarmush ML, Stylopoulos N. Reprogramming of intestinal glucose metabolism and glycemic control in rats after gastric bypass. Science. 2013 Jul 26; 341(6144):406-10. View abstract
  7. Saeidi N, Nestoridi E, Kucharczyk J, Uygun MK, Yarmush ML, Stylopoulos N. Sleeve gastrectomy and Roux-en-Y gastric bypass exhibit differential effects on food preferences, nutrient absorption and energy expenditure in obese rats. Int J Obes (Lond). 2012 Nov; 36(11):1396-402. View abstract
  8. Muñoz R, Carmody JS, Stylopoulos N, Davis P, Kaplan LM. Isolated duodenal exclusion increases energy expenditure and improves glucose homeostasis in diet-induced obese rats. Am J Physiol Regul Integr Comp Physiol. 2012 Nov 15; 303(10):R985-93. View abstract
  9. Hatoum IJ, Stylopoulos N, Vanhoose AM, Boyd KL, Yin DP, Ellacott KL, Ma LL, Blaszczyk K, Keogh JM, Cone RD, Farooqi IS, Kaplan LM. Melanocortin-4 receptor signaling is required for weight loss after gastric bypass surgery. J Clin Endocrinol Metab. 2012 Jun; 97(6):E1023-31. View abstract
  10. Nestoridi E, Kvas S, Kucharczyk J, Stylopoulos N. Resting energy expenditure and energetic cost of feeding are augmented after Roux-en-Y gastric bypass in obese mice. Endocrinology. 2012 May; 153(5):2234-44. View abstract
  11. Kucharczyk J, Nestoridi E, Kvas S, Andrews R, Stylopoulos N. Probing the mechanisms of the metabolic effects of weight loss surgery in humans using a novel mouse model system. J Surg Res. 2013 Jan; 179(1):e91-8. View abstract
  12. Stylopoulos N, Aguirre V. Mechanisms of bariatric surgery and implications for the development of endoluminal therapies for obesity. Gastrointest Endosc. 2009 Dec; 70(6):1167-75. View abstract
  13. Stylopoulos N, Hoppin AG, Kaplan LM. Roux-en-Y gastric bypass enhances energy expenditure and extends lifespan in diet-induced obese rats. Obesity (Silver Spring). 2009 Oct; 17(10):1839-47. View abstract
  14. Aguirre V, Stylopoulos N, Grinbaum R, Kaplan LM. An endoluminal sleeve induces substantial weight loss and normalizes glucose homeostasis in rats with diet-induced obesity. Obesity (Silver Spring). 2008 Dec; 16(12):2585-92. View abstract
  15. Estépar RS, Stylopoulos N, Ellis R, Samset E, Westin CF, Thompson C, Vosburgh K. Towards scarless surgery: an endoscopic ultrasound navigation system for transgastric access procedures. Comput Aided Surg. 2007 Nov; 12(6):311-24. View abstract
  16. Stylopoulos N, Vosburgh KG. Assessing technical skill in surgery and endoscopy: a set of metrics and an algorithm (C-PASS) to assess skills in surgical and endoscopic procedures. Surg Innov. 2007 Jun; 14(2):113-21. View abstract
  17. Vosburgh KG, Stylopoulos N, Estepar RS, Ellis RE, Samset E, Thompson CC. EUS with CT improves efficiency and structure identification over conventional EUS. Gastrointest Endosc. 2007 May; 65(6):866-70. View abstract
  18. San José Estépar R, Stylopoulos N, Ellis RE, Samset E, Westin CF, Thompson C, Vosburgh K. Towards scarless surgery: an endoscopic-ultrasound navigation system for transgastric access procedures. Med Image Comput Comput Assist Interv. 2006; 9(Pt 1):445-53. View abstract
  19. Maithel SK, Stylopoulos N, Jones DB, Rattner DW, Kaplan LM. Intact Leptin Signaling Is Required for Changes in Food Intake After Roux-en-Y Gastric Bypass in Rats. Digestive Disease Week, Chicago, IL, USA. 2005. View abstract
  20. Stylopoulos N, Davis P, Pettit JD, Rattner DW, Kaplan LM. Changes in serum ghrelin predict weight loss after Roux-en-Y gastric bypass in rats. Surg Endosc. 2005 Jul; 19(7):942-6. View abstract
  21. Stylopoulos N, Rattner DW. The history of hiatal hernia surgery: from Bowditch to laparoscopy. Ann Surg. 2005 Jan; 241(1):185-93. View abstract
  22. Maithel SK, Villegas L, Stylopoulos N, Dawson S, Jones DB. Simulated laparoscopy using a head-mounted display vs traditional video monitor: an assessment of performance and muscle fatigue. Surg Endosc. 2005 Mar; 19(3):406-11. View abstract
  23. Stylopoulos N, Cotin S, Maithel SK, Ottensmeye M, Jackson PG, Bardsley RS, Neumann PF, Rattner DW, Dawson SL. Computer-enhanced laparoscopic training system (CELTS): bridging the gap. Surg Endosc. 2004 May; 18(5):782-9. View abstract
  24. Stylopoulos N, Rattner D. Robotics and ergonomics. Surg Clin North Am. 2003 Dec; 83(6):1321-37. View abstract
  25. Sims C, Stylopoulos N, Clavijo J, Barlow C. Histologic characteristics of laparoscopic saline-enhanced electrosurgery of liver and splenic injuries. Surg Endosc. 2003 Nov; 17(11):1739-43. View abstract
  26. Cotin S, Stylopoulos N, Neumann P, Ottensmeyer M, Dawson S. A software based implementation for evaluating surgical performance using kinematic analysis. 2003. View abstract
  27. Stylopoulos N, Cotin S, Dawson S, Ottensmeyer M, Neumann P, Bardsley R, Russell M, Jackson P, Rattner D. CELTS: a clinically-based Computer Enhanced Laparoscopic Training System. Stud Health Technol Inform. 2003; 94:336-42. View abstract
  28. Stylopoulos N, Rattner DW. Paraesophageal hernia: when to operate? Adv Surg. 2003; 37:213-29. View abstract
  29. Stylopoulos N, Gazelle GS, Rattner DW. A cost--utility analysis of treatment options for inguinal hernia in 1,513,008 adult patients. Surg Endosc. 2003 Feb; 17(2):180-9. View abstract
  30. Stylopoulos N, Gazelle GS, Rattner DW. Paraesophageal hernias: operation or observation? Ann Surg. 2002 Oct; 236(4):492-500; discussion 500-1. View abstract
  31. Stylopoulos N, Bunker CJ, Rattner DW. Development of achalasia secondary to laparoscopic Nissen fundoplication. J Gastrointest Surg. 2002 May-Jun; 6(3):368-76; discussion 377-78. View abstract
  32. Tay BK, Stylopoulos N, De S, Rattner DW, Srinivasan MA. Measurement of in-vivo force response of intra-abdominal soft tissues for surgical simulation. Stud Health Technol Inform. 2002; 85:514-9. View abstract
  33. Linos DA, Stylopoulos N. How accurate is computed tomography in predicting the real size of adrenal tumors? A retrospective study. Arch Surg. 1997 Jul; 132(7):740-3. View abstract
  34. Linos DA, Stylopoulos N, Boukis M, Souvatzoglou A, Raptis S, Papadimitriou J. Anterior, posterior, or laparoscopic approach for the management of adrenal diseases? Am J Surg. 1997 Feb; 173(2):120-5. View abstract
  35. Linos DA, Stylopoulos N, Raptis SA. Adrenaloma: a call for more aggressive management. World J Surg. 1996 Sep; 20(7):788-92; discussion 792-3. View abstract
  36. Ferreiro JA, Stylopoulos N. Oncocytic differentiation in salivary gland tumours. J Laryngol Otol. 1995 Jun; 109(6):569-71. View abstract