Research Overview

Dr. Pollock's research program bridges infectious diseases and clinical microbiology, with projects focused on the development and evaluation of novel diagnostic tests for infectious diseases and related applications.  Current research projects include the following:

  • development of a urine antigen detection test for diagnosis of active tuberculosis (collaboration with Dr. Antonio Campos-Neto, Forsyth Institute)
  • development of ultrasensitive and quantitative assays for C. difficile toxins A and B using single molecule array (SiMoA) technology (collaboration with Quanterix Corporation, Lexington, MA, and Ciaran Kelly, BIDMC)
  • development of a paper-based point-of-care "liver function" test using fingerstick blood (collaboration with Diagnostics For All, Cambridge, MA, and Dr. George Whitesides, Harvard Chemistry)
  • development of a SiMoA assay for quantification of HIV p24 protein as a measure of viral load (collaboration with Quanterix Corporation, Lexington, MA)
  • development of a novel device for collection and incremental dispensation of fingerstick blood (collaboration with Dr. Alexis Sauer-Budge, Fraunhofer CMI)

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Research Departments

PUBLICATIONS

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  1. Pollock NR, Banz A, Chen X, Williams D, Xu H, Cuddemi CA, Cui AX, Perrotta M, Alhassan E, Riou B, Lantz A, Miller MA, Kelly CP. Comparison of C. difficile Stool Toxin Concentrations in Adults with Symptomatic Infection and Asymptomatic Carriage using an Ultrasensitive Quantitative Immunoassay. Clin Infect Dis. 2018 May 17. View abstract
  2. Roldan GA, Cui AX, Pollock NR. Assessing the Burden of Clostridium difficile Infection in Low- and Middle-Income Countries. J Clin Microbiol. 2018 Mar; 56(3). View abstract
  3. Pollock N, Dhiman R, Daifalla N, Farhat M, Campos-Neto A. Discovery of a unique Mycobacterium tuberculosis protein through proteomic analysis of urine from patients with active tuberculosis. Microbes Infect. 2018 04; 20(4):228-235. View abstract
  4. Rajasingham R, Pollock NR, Linas BP. The Cost-effectiveness of a Point-of-Care Paper Transaminase Test for Monitoring Treatment of HIV/TB Co-Infected Persons. Open Forum Infect Dis. 2017; 4(4):ofx194. View abstract
  5. Sauer-Budge AF, Brookfield SJ, Janzen R, McGray S, Boardman A, Wirz H, Pollock NR. A novel device for collecting and dispensing fingerstick blood for point of care testing. PLoS One. 2017; 12(8):e0183625. View abstract
  6. Alawdah LS, Campbell JN, Pollock N, Watnick PI. Erysipelothrix rhusiopathiae Suppurative Arthritis in a 12-year-old Boy After an Unusual Fresh Water Exposure. Pediatr Infect Dis J. 2017 04; 36(4):431-433. View abstract
  7. Pollock NR, Wonderly B. Evaluating Novel Diagnostics in an Outbreak Setting: Lessons Learned from Ebola. J Clin Microbiol. 2017 05; 55(5):1255-1261. View abstract
  8. Broadhurst MJ, Brooks TJ, Pollock NR. Diagnosis of Ebola Virus Disease: Past, Present, and Future. Clin Microbiol Rev. 2016 Oct; 29(4):773-93. View abstract
  9. Semper AE, Broadhurst MJ, Richards J, Foster GM, Simpson AJ, Logue CH, Kelly JD, Miller A, Brooks TJ, Murray M, Pollock NR. Performance of the GeneXpert Ebola Assay for Diagnosis of Ebola Virus Disease in Sierra Leone: A Field Evaluation Study. PLoS Med. 2016 Mar; 13(3):e1001980. View abstract
  10. Pollock NR. Ultrasensitive Detection and Quantification of Toxins for Optimized Diagnosis of Clostridium difficile Infection. J Clin Microbiol. 2016 Feb; 54(2):259-64. View abstract
  11. Semper A, Broadhurst MJ, Richards J, Foster GM, Simpson A, Groppelli E, Johnson J, Kelly JD, Brooks T, Logue CH, Miller A, Murray M, Pollock NR. Field Laboratory Evaluation of the GeneXpert Ebola Assay for Diagnosis of Ebola Virus Disease in Sierra Leone. Open Forum Infect Dis. 2015 Dec; 2(Suppl 1). View abstract
  12. Broadhurst MJ, Semper A, Bailey D, Pollock NR. ReEBOV Antigen Rapid Test kit for Ebola - Authors' reply. Lancet. 2015 Dec 05; 386(10010):2255-6. View abstract
  13. Lipsett SC, Pollock NR, Branda JA, Gordon CD, Gordon CR, Lantos PM, Nigrovic LE. The Positive Predictive Value of Lyme Elisa for the Diagnosis of Lyme Disease in Children. Pediatr Infect Dis J. 2015 Nov; 34(11):1260-2. View abstract
  14. Song L, Zhao M, Duffy DC, Hansen J, Shields K, Wungjiranirun M, Chen X, Xu H, Leffler DA, Sambol SP, Gerding DN, Kelly CP, Pollock NR. Development and Validation of Digital Enzyme-Linked Immunosorbent Assays for Ultrasensitive Detection and Quantification of Clostridium difficile Toxins in Stool. J Clin Microbiol. 2015 Oct; 53(10):3204-12. View abstract
  15. Rodriguez NM, Linnes JC, Fan A, Ellenson CK, Pollock NR, Klapperich CM. Paper-Based RNA Extraction, in Situ Isothermal Amplification, and Lateral Flow Detection for Low-Cost, Rapid Diagnosis of Influenza A (H1N1) from Clinical Specimens. Anal Chem. 2015 Aug 04; 87(15):7872-9. View abstract
  16. Broadhurst MJ, Kelly JD, Miller A, Semper A, Bailey D, Groppelli E, Simpson A, Brooks T, Hula S, Nyoni W, Sankoh AB, Kanu S, Jalloh A, Ton Q, Sarchet N, George P, Perkins MD, Wonderly B, Murray M, Pollock NR. ReEBOV Antigen Rapid Test kit for point-of-care and laboratory-based testing for Ebola virus disease: a field validation study. Lancet. 2015 Aug 29; 386(9996):867-74. View abstract
  17. Jain S, Rajasingham R, Noubary F, Coonahan E, Schoeplein R, Baden R, Curry M, Afdhal N, Kumar S, Pollock NR. Performance of an Optimized Paper-Based Test for Rapid Visual Measurement of Alanine Aminotransferase (ALT) in Fingerstick and Venipuncture Samples. PLoS One. 2015; 10(5):e0128118. View abstract
  18. Pollock NR, Song L, Zhao M, Duffy DC, Chen X, Sambol SP, Gerding DN, Kelly CP. Differential immunodetection of toxin B from highly virulent Clostridium difficile BI/NAP-1/027. J Clin Microbiol. 2015 May; 53(5):1705-8. View abstract
  19. Denkinger CM, Kalantri Y, Schumacher SG, Michael JS, Shankar D, Saxena A, Sriram N, Balamugesh T, Luo R, Pollock NR, Pai M, Christopher DJ. Challenges in the development of an immunochromatographic interferon-gamma test for diagnosis of pleural tuberculosis. PLoS One. 2013; 8(12):e85447. View abstract
  20. Pollock NR, McAdam AJ, Pai M, Nardell EA, Bernardo J, Banaei N, Mobo J. Interferon ?-release assays for diagnosis of latent tuberculosis in healthcare workers in low-incidence settings: pros and cons. Clin Chem. 2014 May; 60(5):714-8. View abstract
  21. Pollock NR, McGray S, Colby DJ, Noubary F, Nguyen H, Nguyen TA, Khormaee S, Jain S, Hawkins K, Kumar S, Rolland JP, Beattie PD, Chau NV, Quang VM, Barfield C, Tietje K, Steele M, Weigl BH. Field evaluation of a prototype paper-based point-of-care fingerstick transaminase test. PLoS One. 2013; 8(9):e75616. View abstract
  22. Christopher DJ, Schumacher SG, Michael JS, Luo R, Balamugesh T, Duraikannan P, Pollock NR, Pai M, Denkinger CM. Performance of Xpert MTB/RIF on pleural tissue for the diagnosis of pleural tuberculosis. Eur Respir J. 2013 Nov; 42(5):1427-9. View abstract
  23. Pollock NR, Colby D, Rolland JP. A point-of-care paper-based fingerstick transaminase test: toward low-cost "lab-on-a-chip" technology for the developing world. Clin Gastroenterol Hepatol. 2013 May; 11(5):478-82. View abstract
  24. Zhang JY, Mahalanabis M, Liu L, Chang J, Pollock NR, Klapperich CM. A Disposable Microfluidic Virus Concentration Device Based on Evaporation and Interfacial Tension. Diagnostics (Basel). 2013 Mar; 3(1):155-69. View abstract
  25. Pollock NR, Macovei L, Kanunfre K, Dhiman R, Restrepo BI, Zarate I, Pino PA, Mora-Guzman F, Fujiwara RT, Michel G, Kashino SS, Campos-Neto A. Validation of Mycobacterium tuberculosis Rv1681 protein as a diagnostic marker of active pulmonary tuberculosis. J Clin Microbiol. 2013 May; 51(5):1367-73. View abstract
  26. Pollock NR, Colby D, Rolland JP. A point-of-care paper-based fingerstick transaminase test: toward low-cost "lab-on-a-chip" technology for the developing world. Clin Gastroenterol Hepatol. 2013; 5(11):478-82. View abstract
  27. Pollock NR, Macovei L, Kanunfre K, Dhiman R, Restrepo BI, Zarate I, Pino PA, Mora-Guzman F, Fujiwara RT, Michel G, Kashino SS, Campos-Neto A. Validation of Mycobacterium tuberculosis Rv1681 protein as a diagnostic marker of active pulmonary tuberculosis. J Clin Microbiol. 2013; 5(51):1367-73. View abstract
  28. Pollock NR, Rolland JP, Kumar S, Beattie PD, Jain S, Noubary F, Wong VL, Pohlmann RA, Ryan US, Whitesides GM. A paper-based multiplexed transaminase test for low-cost, point-of-care liver function testing. Sci Transl Med. 2012 Sep 19; 4(152):152ra129. View abstract
  29. Cao Q, Mahalanabis M, Chang J, Carey B, Hsieh C, Stanley A, Odell CA, Mitchell P, Feldman J, Pollock NR, Klapperich CM. Microfluidic chip for molecular amplification of influenza A RNA in human respiratory specimens. PLoS One. 2012; 7(3):e33176. View abstract
  30. Cao Q, Mahalanabis M, Chang J, Carey B, Hsieh C, Stanley A, Odell CA, Mitchell P, Feldman J, Pollock NR, Klapperich CM. Microfluidic chip for molecular amplification of influenza A RNA in human respiratory specimens. PLoS One. 2012; 3(7):e33176. View abstract
  31. Eisenberg RL, Pollock NR. Low yield of chest radiography in a large tuberculosis screening program. Radiology. 2010 Sep; 256(3):998-1004. View abstract
  32. Pollock NR, Duong S, Cheng A, Han LL, Smole S, Kirby JE. Ruling out novel H1N1 influenza virus infection with direct fluorescent antigen testing. Clin Infect Dis. 2009 Sep 15; 49(6):e66-8. View abstract
  33. Pollock NR, Kashino SS, Napolitano DR, Sloutsky A, Joshi S, Guillet J, Wong M, Nardell E, Campos-Neto A. Evaluation of the effect of treatment of latent tuberculosis infection on QuantiFERON-TB gold assay results. Infect Control Hosp Epidemiol. 2009 Apr; 30(4):392-5. View abstract
  34. Pollock NR, Campos-Neto A, Kashino S, Napolitano D, Behar SM, Shin D, Sloutsky A, Joshi S, Guillet J, Wong M, Nardell E. Discordant QuantiFERON-TB Gold test results among US healthcare workers with increased risk of latent tuberculosis infection: a problem or solution? Infect Control Hosp Epidemiol. 2008 Sep; 29(9):878-86. View abstract
  35. Pollock, Nira, M.D., Ph.D.; Kashino, Suely, Ph.D.; Campos-Neto, Antonio, M.D., Ph.D. . Development of a Novel Urine Antigen Detection Test for Active Tuberculosis. 43rd Joint Tuberculosis/Leprosy Conference and Diagnostics Workshop . 2008. View abstract
  36. Kashino SS, Pollock N, Napolitano DR, Rodrigues V, Campos-Neto A. Identification and characterization of Mycobacterium tuberculosis antigens in urine of patients with active pulmonary tuberculosis: an innovative and alternative approach of antigen discovery of useful microbial molecules. Clin Exp Immunol. 2008 Jul; 153(1):56-62. View abstract
  37. Napolitano DR, Pollock N, Kashino SS, Rodrigues V, Campos-Neto A. Identification of Mycobacterium tuberculosis ornithine carboamyltransferase in urine as a possible molecular marker of active pulmonary tuberculosis. Clin Vaccine Immunol. 2008 Apr; 15(4):638-43. View abstract
  38. N. Pollock, A. Sloutsky, S. Joshi, M. Wong, and E. Nardell. Screening Health Care Workers (HCW) with Quantiferon-Gold: Possible False Negative Results in HCW with High Pre-Test Probability of Latent Tuberculosis Infection. Presented at the International Union Against Tuberculosis and Lung Disease (IUATLD) Conference and Global Symposium on Interferon-gamma Assays, Vancouver, Canada, February 2007. 2007. View abstract
  39. Pollock N, Westerling J, Sloutsky A. Specimen dilution increases the diagnostic utility of the gen-probe mycobacterium tuberculosis direct test. Am J Clin Pathol. 2006 Jul; 126(1):142-7. View abstract
  40. Pollock N, de Hostos EL, Turck CW, Vale RD. Reconstitution of membrane transport powered by a novel dimeric kinesin motor of the Unc104/KIF1A family purified from Dictyostelium. J Cell Biol. 1999 Nov 01; 147(3):493-506. View abstract
  41. Pollock N. Reconstitution of Microtubule-based Organelle Transport Using Factors Purified from Dictyostelium. 1999. View abstract
  42. Pollock N, Koonce MP, de Hostos EL, Vale RD. In vitro microtubule-based organelle transport in wild-type Dictyostelium and cells overexpressing a truncated dynein heavy chain. Cell Motil Cytoskeleton. 1998; 40(3):304-14. View abstract