Research Overview

Dr. Brugnara’s basic research interests are focused on transport of ions across cell membrane. His studies have identified the role of specific transport proteins in inducing erythrocyte dehydration in sickle cell disease and identified new therapeutic approaches for sickle cell anemia. His most recent research has focused on genetic determinant of erythrocyte hydration and genetic diseases of red cell hydration like dehydrated stomatocytosis.

Dr. Brugnara’s clinical laboratory-based research has been focused on the use of hematological parameters to assess the balance between iron availability and erythropoiesis. He has described the appearance of functional iron deficiency in normal subjects treated with recombinant human erythropoietin (r-HuEPO) based on the particular flow cytometric characteristics of erythrocytes and reticulocytes. He has shown the clinical value of reticulocyte parameters in the setting of r-HuEPO use and in the diagnosis of iron deficiency in children.

About Carlo Brugnara

Dr. Carlo Brugnara received his MD from the University of Verona, Italy in 1979. In 1982 he joined as a postdoctoral fellow the laboratory of Dr. Daniel Tosteson in the Department of Physiology at Harvard Medical School. He trained in Clinical Pathology and Transfusion Medicine at Brigham and Women’s Hospital in Boston from 1986 to 1990, and holds board certifications in both specialties. In 1992, he joined the Department of Laboratory Medicine at Boston Children’s Hospital as Director of the Hematology Laboratory.

Dr. Brugnara is a fellow of the American Society of Clinical Investigation, the Association of American Physicians, and the Academy of Clinical Laboratory Physicians and Scientists. Since 2007 he is the Editor-in-Chief of the American Journal of Hematology.

Researcher Services

Researcher Areas

  • Sickle Cell Disease
  • Erythrocyte physiology
  • Ion transport
  • Iron metabolism

Research Departments

PUBLICATIONS

Publications powered by Harvard Catalyst Profiles

  1. Hosseinzadeh VA, Brugnara C, Holt RG. Shape oscillations of single blood drops: applications to human blood and sickle cell disease. Sci Rep. 2018 Nov 14; 8(1):16794. View abstract
  2. Matte A, Recchiuti A, Federti E, Koehl B, Mintz T, El Nemer W, Tharaux PL, Brousse V, Andolfo I, Lamolinara A, Weinberg O, Siciliano A, Norris PC, Riley IR, Iolascon A, Serhan CN, Brugnara C, De Franceschi L. Resolution of sickle cell disease associated inflammation and tissue damage with 17R-Resolvin D1. Blood. 2018 Nov 07. View abstract
  3. Andolfo I, Russo R, Rosato BE, Manna F, Gambale A, Brugnara C, Iolascon A. Genotype-phenotype correlation and risk stratification in a cohort of 123 hereditary stomatocytosis patients. Am J Hematol. 2018 Dec; 93(12):1509-1517. View abstract
  4. Ilboudo Y, Bartolucci P, Garrett ME, Ashley-Koch A, Telen M, Brugnara C, Galactéros F, Lettre G. A common functional PIEZO1 deletion allele associates with red blood cell density in sickle cell disease patients. Am J Hematol. 2018 Nov; 93(11):E362-E365. View abstract
  5. Egan ES, Weekes MP, Kanjee U, Manzo J, Srinivasan A, Lomas-Francis C, Westhoff C, Takahashi J, Tanaka M, Watanabe S, Brugnara C, Gygi SP, Tani Y, Duraisingh MT. Erythrocytes lacking the Langereis blood group protein ABCB6 are resistant to the malaria parasite Plasmodium falciparum. Commun Biol. 2018; 1:45. View abstract
  6. Wish JB, Aronoff GR, Bacon BR, Brugnara C, Eckardt KU, Ganz T, Macdougall IC, Núñez J, Perahia AJ, Wood JC. Positive Iron Balance in Chronic Kidney Disease: How Much is Too Much and How to Tell? Am J Nephrol. 2018; 47(2):72-83. View abstract
  7. Brugnara C. Sickle cell dehydration: Pathophysiology and therapeutic applications. Clin Hemorheol Microcirc. 2018; 68(2-3):187-204. View abstract
  8. Pedlar CR, Brugnara C, Bruinvels G, Burden R. Iron balance and iron supplementation for the female athlete: A practical approach. Eur J Sport Sci. 2018 Mar; 18(2):295-305. View abstract
  9. Kaufman HW, Niles JK, Gallagher DR, Rivera A, Alper SL, Brugnara C, Snyder LM. Revised prevalence estimate of possible Hereditary Xerocytosis as derived from a large U.S. Laboratory database. Am J Hematol. 2018 01; 93(1):E9-E12. View abstract
  10. Hod EA, Brugnara C, Pilichowska M, Sandhaus LM, Luu HS, Forest SK, Netterwald JC, Reynafarje GM, Kratz A. Automated cell counts on CSF samples: A multicenter performance evaluation of the GloCyte system. Int J Lab Hematol. 2018 Feb; 40(1):56-65. View abstract
  11. Ilboudo Y, Bartolucci P, Rivera A, Sedzro JC, Beaudoin M, Trudel M, Alper SL, Brugnara C, Galactéros F, Lettre G. Genome-wide association study of erythrocyte density in sickle cell disease patients. Blood Cells Mol Dis. 2017 06; 65:60-65. View abstract
  12. Rivera A, Vandorpe DH, Shmukler BE, Gallagher DR, Fikry CC, Kuypers FA, Brugnara C, Snyder LM, Alper SL. Erythrocytes from hereditary xerocytosis patients heterozygous for KCNN4 V282M exhibit increased spontaneous Gardos channel-like activity inhibited by senicapoc. Am J Hematol. 2017 06; 92(6):E108-E110. View abstract
  13. Canver MC, Lessard S, Pinello L, Wu Y, Ilboudo Y, Stern EN, Needleman AJ, Galactéros F, Brugnara C, Kutlar A, McKenzie C, Reid M, Chen DD, Das PP, A Cole M, Zeng J, Kurita R, Nakamura Y, Yuan GC, Lettre G, Bauer DE, Orkin SH. Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci. Nat Genet. 2017 Apr; 49(4):625-634. View abstract
  14. Hennek JW, Kumar AA, Wiltschko AB, Patton MR, Lee SY, Brugnara C, Adams RP, Whitesides GM. Diagnosis of iron deficiency anemia using density-based fractionation of red blood cells. Lab Chip. 2016 10 05; 16(20):3929-3939. View abstract
  15. Andolfo I, Russo R, Manna F, De Rosa G, Gambale A, Zouwail S, Detta N, Pardo CL, Alper SL, Brugnara C, Sharma AK, De Franceschi L, Iolascon A. Functional characterization of novel ABCB6 mutations and their clinical implications in familial pseudohyperkalemia. Haematologica. 2016 08; 101(8):909-17. View abstract
  16. Brugnara C. The American Journal of Hematology turns 40. Am J Hematol. 2016 Jan; 91(1):4. View abstract
  17. Giani FC, Fiorini C, Wakabayashi A, Ludwig LS, Salem RM, Jobaliya CD, Regan SN, Ulirsch JC, Liang G, Steinberg-Shemer O, Guo MH, Esko T, Tong W, Brugnara C, Hirschhorn JN, Weiss MJ, Zon LI, Chou ST, French DL, Musunuru K, Sankaran VG. Targeted Application of Human Genetic Variation Can Improve Red Blood Cell Production from Stem Cells. Cell Stem Cell. 2016 Jan 07; 18(1):73-78. View abstract
  18. Tubman VN, Mejia P, Shmukler BE, Bei AK, Alper SL, Mitchell JR, Brugnara C, Duraisingh MT. The Clinically Tested Gardos Channel Inhibitor Senicapoc Exhibits Antimalarial Activity. Antimicrob Agents Chemother. 2016 01; 60(1):613-6. View abstract
  19. Archer N, Galacteros F, Brugnara C. 2015 Clinical trials update in sickle cell anemia. Am J Hematol. 2015 Oct; 90(10):934-50. View abstract
  20. Andolfo I, Russo R, Manna F, Shmukler BE, Gambale A, Vitiello G, De Rosa G, Brugnara C, Alper SL, Snyder LM, Iolascon A. Novel Gardos channel mutations linked to dehydrated hereditary stomatocytosis (xerocytosis). Am J Hematol. 2015 Oct; 90(10):921-6. View abstract
  21. Archer NM, Brugnara C. Diagnosis of iron-deficient states. Crit Rev Clin Lab Sci. 2015; 52(5):256-72. View abstract
  22. Archer NM, Brugnara C. Diagnosis of iron-deficient states. Crit Rev Clin Lab Sci. 2015 Aug 14; 1-17. View abstract
  23. Boscolo E, Limaye N, Huang L, Kang KT, Soblet J, Uebelhoer M, Mendola A, Natynki M, Seront E, Dupont S, Hammer J, Legrand C, Brugnara C, Eklund L, Vikkula M, Bischoff J, Boon LM. Rapamycin improves TIE2-mutated venous malformation in murine model and human subjects. J Clin Invest. 2015 Sep; 125(9):3491-504. View abstract
  24. Egan ES, Jiang RH, Moechtar MA, Barteneva NS, Weekes MP, Nobre LV, Gygi SP, Paulo JA, Frantzreb C, Tani Y, Takahashi J, Watanabe S, Goldberg J, Paul AS, Brugnara C, Root DE, Wiegand RC, Doench JG, Duraisingh MT. Malaria. A forward genetic screen identifies erythrocyte CD55 as essential for Plasmodium falciparum invasion. Science. 2015 May 08; 348(6235):711-4. View abstract
  25. Kalish BT, Matte A, Andolfo I, Iolascon A, Weinberg O, Ghigo A, Cimino J, Siciliano A, Hirsch E, Federti E, Puder M, Brugnara C, De Franceschi L. Dietary ?-3 fatty acids protect against vasculopathy in a transgenic mouse model of sickle cell disease. Haematologica. 2015 Jul; 100(7):870-80. View abstract
  26. Archer NM, Samnaliev M, Grace R, Brugnara C. The utility of the DDAVP challenge test in children with low von Willebrand factor. Br J Haematol. 2015 Sep; 170(6):884-6. View abstract
  27. Brugnara C. American Journal of Hematology, getting ready for the 40th birthday in 2016. Am J Hematol. 2015 Jan; 90(1):1. View abstract
  28. Sharma S, Brugnara C, Betensky RA, Waikar SS. Reductions in red blood cell 2,3-diphosphoglycerate concentration during continuous renal replacment therapy. Clin J Am Soc Nephrol. 2015 Jan 07; 10(1):74-9. View abstract
  29. Kumar AA, Chunda-Liyoka C, Hennek JW, Mantina H, Lee SY, Patton MR, Sambo P, Sinyangwe S, Kankasa C, Chintu C, Brugnara C, Stossel TP, Whitesides GM. Evaluation of a density-based rapid diagnostic test for sickle cell disease in a clinical setting in Zambia. PLoS One. 2014; 9(12):e114540. View abstract
  30. Kratz A, Brugnara C. Automated hematology analyzers: state of the art. Clin Lab Med. 2015 Mar; 35(1):xiii-xiv. View abstract
  31. Piva E, Brugnara C, Spolaore F, Plebani M. Clinical utility of reticulocyte parameters. Clin Lab Med. 2015 Mar; 35(1):133-63. View abstract
  32. Bhaduri B, Kandel M, Brugnara C, Tangella K, Popescu G. Optical assay of erythrocyte function in banked blood. Sci Rep. 2014 Sep 05; 4:6211. View abstract
  33. Kumar AA, Patton MR, Hennek JW, Lee SY, D'Alesio-Spina G, Yang X, Kanter J, Shevkoplyas SS, Brugnara C, Whitesides GM. Density-based separation in multiphase systems provides a simple method to identify sickle cell disease. Proc Natl Acad Sci U S A. 2014 Oct 14; 111(41):14864-9. View abstract
  34. Zhang X, Campreciós G, Rimmelé P, Liang R, Yalcin S, Mungamuri SK, Barminko J, D'Escamard V, Baron MH, Brugnara C, Papatsenko D, Rivella S, Ghaffari S. FOXO3-mTOR metabolic cooperation in the regulation of erythroid cell maturation and homeostasis. Am J Hematol. 2014 Oct; 89(10):954-63. View abstract
  35. Archer NM, Shmukler BE, Andolfo I, Vandorpe DH, Gnanasambandam R, Higgins JM, Rivera A, Fleming MD, Sachs F, Gottlieb PA, Iolascon A, Brugnara C, Alper SL, Nathan DG. Hereditary xerocytosis revisited. Am J Hematol. 2014 Dec; 89(12):1142-6. View abstract
  36. Rimmelé P, Bigarella CL, Liang R, Izac B, Dieguez-Gonzalez R, Barbet G, Donovan M, Brugnara C, Blander JM, Sinclair DA, Ghaffari S. Aging-like phenotype and defective lineage specification in SIRT1-deleted hematopoietic stem and progenitor cells. Stem Cell Reports. 2014 Jul 08; 3(1):44-59. View abstract
  37. Darbari DS, Nouraie M, Taylor JG, Brugnara C, Castro O, Ballas SK. Alpha-thalassaemia and response to hydroxyurea in sickle cell anaemia. Eur J Haematol. 2014 Apr; 92(4):341-5. View abstract
  38. Shmukler BE, Vandorpe DH, Rivera A, Auerbach M, Brugnara C, Alper SL. Dehydrated stomatocytic anemia due to the heterozygous mutation R2456H in the mechanosensitive cation channel PIEZO1: a case report. Blood Cells Mol Dis. 2014 Jan; 52(1):53-4. View abstract
  39. Franco SS, De Falco L, Ghaffari S, Brugnara C, Sinclair DA, Matte' A, Iolascon A, Mohandas N, Bertoldi M, An X, Siciliano A, Rimmelé P, Cappellini MD, Michan S, Zoratti E, Anne J, De Franceschi L. Resveratrol accelerates erythroid maturation by activation of FoxO3 and ameliorates anemia in beta-thalassemic mice. Haematologica. 2014 Feb; 99(2):267-75. View abstract
  40. Brugnara C, Mohandas N. Red cell indices in classification and treatment of anemias: from M.M. Wintrobes's original 1934 classification to the third millennium. Curr Opin Hematol. 2013 May; 20(3):222-30. View abstract
  41. Rivera A, Zee RY, Alper SL, Peters LL, Brugnara C. Strain-specific variations in cation content and transport in mouse erythrocytes. Physiol Genomics. 2013 May 01; 45(9):343-50. View abstract
  42. Andolfo I, Alper SL, De Franceschi L, Auriemma C, Russo R, De Falco L, Vallefuoco F, Esposito MR, Vandorpe DH, Shmukler BE, Narayan R, Montanaro D, D'Armiento M, Vetro A, Limongelli I, Zuffardi O, Glader BE, Schrier SL, Brugnara C, Stewart GW, Delaunay J, Iolascon A. Multiple clinical forms of dehydrated hereditary stomatocytosis arise from mutations in PIEZO1. Blood. 2013 May 09; 121(19):3925-35, S1-12. View abstract
  43. Lim C, Hansen E, DeSimone TM, Moreno Y, Junker K, Bei A, Brugnara C, Buckee CO, Duraisingh MT. Expansion of host cellular niche can drive adaptation of a zoonotic malaria parasite to humans. Nat Commun. 2013; 4:1638. View abstract
  44. Andolfo I, Alper SL, Delaunay J, Auriemma C, Russo R, Asci R, Esposito MR, Sharma AK, Shmukler BE, Brugnara C, De Franceschi L, Iolascon A. Missense mutations in the ABCB6 transporter cause dominant familial pseudohyperkalemia. Am J Hematol. 2013 Jan; 88(1):66-72. View abstract
  45. Wieschhaus A, Khan A, Zaidi A, Rogalin H, Hanada T, Liu F, De Franceschi L, Brugnara C, Rivera A, Chishti AH. Calpain-1 knockout reveals broad effects on erythrocyte deformability and physiology. Biochem J. 2012 Nov 15; 448(1):141-52. View abstract
  46. Brugnara C, Adamson J, Auerbach M, Kane R, Macdougall I, Mast A. Iron deficiency: what are the future trends in diagnostics and therapeutics? Clin Chem. 2013 May; 59(5):740-5. View abstract
  47. Shah DI, Takahashi-Makise N, Cooney JD, Li L, Schultz IJ, Pierce EL, Narla A, Seguin A, Hattangadi SM, Medlock AE, Langer NB, Dailey TA, Hurst SN, Faccenda D, Wiwczar JM, Heggers SK, Vogin G, Chen W, Chen C, Campagna DR, Brugnara C, Zhou Y, Ebert BL, Danial NN, Fleming MD, Ward DM, Campanella M, Dailey HA, Kaplan J, Paw BH. Mitochondrial Atpif1 regulates haem synthesis in developing erythroblasts. Nature. 2012 Nov 22; 491(7425):608-12. View abstract
  48. De Franceschi L, Franco RS, Bertoldi M, Brugnara C, Matté A, Siciliano A, Wieschhaus AJ, Chishti AH, Joiner CH. Pharmacological inhibition of calpain-1 prevents red cell dehydration and reduces Gardos channel activity in a mouse model of sickle cell disease. FASEB J. 2013 Feb; 27(2):750-9. View abstract
  49. Bartolucci P, Brugnara C, Teixeira-Pinto A, Pissard S, Moradkhani K, Jouault H, Galacteros F. Erythrocyte density in sickle cell syndromes is associated with specific clinical manifestations and hemolysis. Blood. 2012 Oct 11; 120(15):3136-41. View abstract
  50. Kheir JN, Scharp LA, Borden MA, Swanson EJ, Loxley A, Reese JH, Black KJ, Velazquez LA, Thomson LM, Walsh BK, Mullen KE, Graham DA, Lawlor MW, Brugnara C, Bell DC, McGowan FX. Oxygen gas-filled microparticles provide intravenous oxygen delivery. Sci Transl Med. 2012 Jun 27; 4(140):140ra88. View abstract
  51. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A, Delanghe J. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Am J Hematol. 2012 Apr; 87(4):347-8. View abstract
  52. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Transfusion. 2012 Jun; 52(6):e17-9. View abstract
  53. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A, Delanghe J. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Clin Chem Lab Med. 2012 Mar; 50(3):411-3. View abstract
  54. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A, Delanghe J. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Scand J Clin Lab Invest. 2012 Apr; 72(2):89-91. View abstract
  55. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Clin Biochem. 2012 Feb; 45(3):185-6. View abstract
  56. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A, Delanghe J. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Statement by the Consortium of Laboratory Medicine Journal Editors. Ann Clin Biochem. 2012 Mar; 49(Pt 2):105-7. View abstract
  57. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A, Delanghe J. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Clin Chem. 2012 Mar; 58(3):483-5. View abstract
  58. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A, Delanghe J. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Clin Chim Acta. 2012 Apr 11; 413(7-8):653-5. View abstract
  59. Stewart AK, Shmukler BE, Vandorpe DH, Rivera A, Heneghan JF, Li X, Hsu A, Karpatkin M, O'Neill AF, Bauer DE, Heeney MM, John K, Kuypers FA, Gallagher PG, Lux SE, Brugnara C, Westhoff CM, Alper SL. Loss-of-function and gain-of-function phenotypes of stomatocytosis mutant RhAG F65S. Am J Physiol Cell Physiol. 2011 Dec; 301(6):C1325-43. View abstract
  60. Rifai N, Plebani M, Wu A, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full-disclosure in industry-sponsored laboratory medicine research studies: Statement by the Consortium of Laboratory Medicine Journal Editors. Am J Hematol. 2011 Mar; 86(3):244. View abstract
  61. Wang W, Brugnara C, Snyder C, Wynn L, Rogers Z, Kalinyak K, Brown C, Qureshi A, Bigelow C, Neumayr L, Smith-Whitley K, Chui DH, Delahunty M, Woolson R, Steinberg M, Telen M, Kesler K. The effects of hydroxycarbamide and magnesium on haemoglobin SC disease: results of the multi-centre CHAMPS trial. Br J Haematol. 2011 Mar; 152(6):771-6. View abstract
  62. Rifai N, Plebani M, Wu A, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full disclosure in industry-sponsored laboratory medicine research studies: statement by the Consortium of Laboratory Medicine Journal Editors. Ann Clin Biochem. 2011 Jan; 48(Pt 1):5-6. View abstract
  63. Stewart AK, Kedar PS, Shmukler BE, Vandorpe DH, Hsu A, Glader B, Rivera A, Brugnara C, Alper SL. Functional characterization and modified rescue of novel AE1 mutation R730C associated with overhydrated cation leak stomatocytosis. Am J Physiol Cell Physiol. 2011 May; 300(5):C1034-46. View abstract
  64. Rifai N, Plebani M, Wu A, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full disclosure in industry-sponsored laboratory medicine research studies: statement by the Consortium of Laboratory Medicine Journal Editors. Transfusion. 2012 Jun; 52(6):e15-6. View abstract
  65. Rifai N, Plebani M, Wu AH, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full disclosure in industry-sponsored laboratory medicine research studies: Statement by the Consortium of Laboratory Medicine Journal Editors. Clin Biochem. 2011 Feb; 44(2-3):149-50. View abstract
  66. Rifai N, Plebani M, Wu A, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full-disclosure in industry-sponsored laboratory medicine research studies: statement by the Consortium of Laboratory Medicine Journal Editors. Scand J Clin Lab Invest. 2011 May; 71(3):177-8. View abstract
  67. Rifai N, Plebani M, Wu A, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full disclosure in industry-sponsored laboratory medicine research studies: statement by the Consortium of Laboratory Medicine Journal Editors. Clin Chem. 2011 Mar; 57(3):359-60. View abstract
  68. Rifai N, Plebani M, Wu A, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full-disclosure in industry-sponsored laboratory medicine research studies: statement by the Consortium of Laboratory Medicine Journal Editors. Clin Chem Lab Med. 2011 Jan; 49(1):3-4. View abstract
  69. Rifai N, Plebani M, Wu AH, Delanghe J, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full-disclosure in industry-sponsored laboratory medicine research studies: statement by the Consortium of Laboratory Medicine Journal Editors. Clin Chim Acta. 2011 Mar 18; 412(7-8):491-2. View abstract
  70. Bei AK, Brugnara C, Duraisingh MT. In vitro genetic analysis of an erythrocyte determinant of malaria infection. J Infect Dis. 2010 Dec 01; 202(11):1722-7. View abstract
  71. Peters LL, Shavit JA, Lambert AJ, Tsaih SW, Li Q, Su Z, Leduc MS, Paigen B, Churchill GA, Ginsburg D, Brugnara C. Sequence variation at multiple loci influences red cell hemoglobin concentration. Blood. 2010 Dec 16; 116(25):e139-49. View abstract
  72. Piva E, Brugnara C, Chiandetti L, Plebani M. Automated reticulocyte counting: state of the art and clinical applications in the evaluation of erythropoiesis. Clin Chem Lab Med. 2010 Oct; 48(10):1369-80. View abstract
  73. Vandorpe DH, Xu C, Shmukler BE, Otterbein LE, Trudel M, Sachs F, Gottlieb PA, Brugnara C, Alper SL. Hypoxia activates a Ca2+-permeable cation conductance sensitive to carbon monoxide and to GsMTx-4 in human and mouse sickle erythrocytes. PLoS One. 2010 Jan 15; 5(1):e8732. View abstract
  74. Ballas SK, McCarthy WF, Guo N, Brugnara C, Kling G, Bauserman RL, Waclawiw MA. Early detection of response to hydroxyurea therapy in patients with sickle cell anemia. Hemoglobin. 2010; 34(5):424-9. View abstract
  75. Casula S, Zolotarev AS, Stuart-Tilley AK, Wilhelm S, Shmukler BE, Brugnara C, Alper SL. Chemical crosslinking studies with the mouse Kcc1 K-Cl cotransporter. Blood Cells Mol Dis. 2009 May-Jun; 42(3):233-40. View abstract
  76. Brugnara C. An immunoassay for human serum hepcidin at last: Ganz klar? Blood. 2008 Nov 15; 112(10):3922-3. View abstract
  77. De Franceschi L, Biondani A, Carta F, Turrini F, Laudanna C, Deana R, Brunati AM, Turretta L, Iolascon A, Perrotta S, Elson A, Bulato C, Brugnara C. PTPepsilon has a critical role in signaling transduction pathways and phosphoprotein network topology in red cells. Proteomics. 2008 Nov; 8(22):4695-708. View abstract
  78. Licameli GR, Jones DT, Santosuosso J, Lapp C, Brugnara C, Kenna MA. Use of a preoperative bleeding questionnaire in pediatric patients who undergo adenotonsillectomy. Otolaryngol Head Neck Surg. 2008 Oct; 139(4):546-550. View abstract
  79. Shaker M, Jenkins P, Ullrich C, Brugnara C, Nghiem BT, Bernstein H. An economic analysis of anemia prevention during infancy. J Pediatr. 2009 Jan; 154(1):44-9. View abstract
  80. Katodritou E, Zervas K, Terpos E, Brugnara C. Use of erythropoiesis stimulating agents and intravenous iron for cancer and treatment-related anaemia: the need for predictors and indicators of effectiveness has not abated. Br J Haematol. 2008 Jul; 142(1):3-10. View abstract
  81. Alper SL, Vandorpe DH, Peters LL, Brugnara C. Reduced DIDS-sensitive chloride conductance in Ae1-/- mouse erythrocytes. Blood Cells Mol Dis. 2008 Jul-Aug; 41(1):22-34. View abstract
  82. De Franceschi L, Platt OS, Malpeli G, Janin A, Scarpa A, Leboeuf C, Beuzard Y, Payen E, Brugnara C. Protective effects of phosphodiesterase-4 (PDE-4) inhibition in the early phase of pulmonary arterial hypertension in transgenic sickle cell mice. FASEB J. 2008 Jun; 22(6):1849-60. View abstract
  83. Hankins JS, Wynn LW, Brugnara C, Hillery CA, Li CS, Wang WC. Phase I study of magnesium pidolate in combination with hydroxycarbamide for children with sickle cell anaemia. Br J Haematol. 2008 Jan; 140(1):80-5. View abstract
  84. Marinkovic D, Zhang X, Yalcin S, Luciano JP, Brugnara C, Huber T, Ghaffari S. Foxo3 is required for the regulation of oxidative stress in erythropoiesis. J Clin Invest. 2007 Aug; 117(8):2133-44. View abstract
  85. Bertin A, Mahaney MC, Cox LA, Rogers J, VandeBerg JL, Brugnara C, Platt OS. Quantitative trait loci for peripheral blood cell counts: a study in baboons. Mamm Genome. 2007 May; 18(5):361-72. View abstract
  86. Rust MB, Alper SL, Rudhard Y, Shmukler BE, Vicente R, Brugnara C, Trudel M, Jentsch TJ, Hübner CA. Disruption of erythroid K-Cl cotransporters alters erythrocyte volume and partially rescues erythrocyte dehydration in SAD mice. J Clin Invest. 2007 Jun; 117(6):1708-17. View abstract
  87. Brugnara C, de Franceschi L. [Clinical trials of new therapeutic pharmacology for sickle cell disease]. Sante. 2006 Oct-Dec; 16(4):263-8. View abstract
  88. Brugnara C, Schiller B, Moran J. Reticulocyte hemoglobin equivalent (Ret He) and assessment of iron-deficient states. Clin Lab Haematol. 2006 Oct; 28(5):303-8. View abstract
  89. Pysher TJ, Bach PR, Geaghan SM, Hamilton MS, Laposata M, Lockitch G, Brugnara C, Coffin CM, Pasquali M, Rinaldo P, Roberts WL, Rutledge JC, Ashwood ER, Blaylock RC, Campos JM, Goldsmith B, Jones PM, Lim M, Meikle AW, Perkins SL, Perry DA, Petti CA, Rogers BB, Steele PE, Weiss RL, Woods G. Teaching pediatric laboratory medicine to pathology residents. Arch Pathol Lab Med. 2006 Jul; 130(7):1031-8. View abstract
  90. Rivera A, De Franceschi L, Peters LL, Gascard P, Mohandas N, Brugnara C. Effect of complete protein 4.1R deficiency on ion transport properties of murine erythrocytes. Am J Physiol Cell Physiol. 2006 Nov; 291(5):C880-6. View abstract
  91. de Franceschi L, Malpeli G, Scarpa A, Janin A, Muchitsch EM, Roncada P, Leboeuf C, Corrocher R, Beuzard Y, Brugnara C. Protective effects of S-nitrosoalbumin on lung injury induced by hypoxia-reoxygenation in mouse model of sickle cell disease. Am J Physiol Lung Cell Mol Physiol. 2006 Sep; 291(3):L457-65. View abstract
  92. Peters LL, Lambert AJ, Zhang W, Churchill GA, Brugnara C, Platt OS. Quantitative trait loci for baseline erythroid traits. Mamm Genome. 2006 Apr; 17(4):298-309. View abstract
  93. Liu Y, Pop R, Sadegh C, Brugnara C, Haase VH, Socolovsky M. Suppression of Fas-FasL coexpression by erythropoietin mediates erythroblast expansion during the erythropoietic stress response in vivo. Blood. 2006 Jul 01; 108(1):123-33. View abstract
  94. De Franceschi L, Villa-Moruzzi E, Biondani A, Siciliano A, Brugnara C, Alper SL, Lowell CA, Berton G. Regulation of K-Cl cotransport by protein phosphatase 1alpha in mouse erythrocytes. Pflugers Arch. 2006 Mar; 451(6):760-8. View abstract
  95. Peters LL, Zhang W, Lambert AJ, Brugnara C, Churchill GA, Platt OS. Quantitative trait loci for baseline white blood cell count, platelet count, and mean platelet volume. Mamm Genome. 2005 Oct; 16(10):749-63. View abstract
  96. Ullrich C, Wu A, Armsby C, Rieber S, Wingerter S, Brugnara C, Shapiro D, Bernstein H. Screening healthy infants for iron deficiency using reticulocyte hemoglobin content. JAMA. 2005 Aug 24; 294(8):924-30. View abstract
  97. Stoffman N, Brugnara C, Woods ER. An algorithm using reticulocyte hemoglobin content (CHr) measurement in screening adolescents for iron deficiency. J Adolesc Health. 2005 Jun; 36(6):529. View abstract
  98. Mahaney MC, Brugnara C, Lease LR, Platt OS. Genetic influences on peripheral blood cell counts: a study in baboons. Blood. 2005 Aug 15; 106(4):1210-4. View abstract
  99. De Franceschi L, Rivera A, Fleming MD, Honczarenko M, Peters LL, Gascard P, Mohandas N, Brugnara C. Evidence for a protective role of the Gardos channel against hemolysis in murine spherocytosis. Blood. 2005 Aug 15; 106(4):1454-9. View abstract
  100. Mallozzi C, De Franceschi L, Brugnara C, Di Stasi AM. Protein phosphatase 1alpha is tyrosine-phosphorylated and inactivated by peroxynitrite in erythrocytes through the src family kinase fgr. Free Radic Biol Med. 2005 Jun 15; 38(12):1625-36. View abstract
  101. Abdulmalik O, Safo MK, Chen Q, Yang J, Brugnara C, Ohene-Frempong K, Abraham DJ, Asakura T. 5-hydroxymethyl-2-furfural modifies intracellular sickle haemoglobin and inhibits sickling of red blood cells. Br J Haematol. 2005 Feb; 128(4):552-61. View abstract
  102. de Franceschi L, Turrini F, Honczarenko M, Ayi K, Rivera A, Fleming MD, Law T, Mannu F, Kuypers FA, Bast A, van der Vijgh WJ, Brugnara C. In vivo reduction of erythrocyte oxidant stress in a murine model of beta-thalassemia. Haematologica. 2004 Nov; 89(11):1287-98. View abstract
  103. Rivera A, Ferreira A, Bertoni D, Romero JR, Brugnara C. Abnormal regulation of Mg2+ transport via Na/Mg exchanger in sickle erythrocytes. Blood. 2005 Jan 01; 105(1):382-6. View abstract
  104. Begenisich T, Nakamoto T, Ovitt CE, Nehrke K, Brugnara C, Alper SL, Melvin JE. Physiological roles of the intermediate conductance, Ca2+-activated potassium channel Kcnn4. J Biol Chem. 2004 Nov 12; 279(46):47681-7. View abstract
  105. Geltman PL, Meyers AF, Mehta SD, Brugnara C, Villon I, Wu YA, Bauchner H. Daily multivitamins with iron to prevent anemia in high-risk infants: a randomized clinical trial. Pediatrics. 2004 Jul; 114(1):86-93. View abstract
  106. Aune MW, Becker JL, Brugnara C, Canfield W, Dorfman DM, Fiehn W, Fischer G, Fitzpatrick P, Flaming TH, Henriksen HK, Kunicka JE, Lackner KJ, Minchello E, Mullenix PA, Myers M, Petersen A, Ternstrom W, Wilson SJ. Automated flow cytometric analysis of blood cells in cerebrospinal fluid: analytic performance. Am J Clin Pathol. 2004 May; 121(5):690-700. View abstract
  107. Warady BA, Kausz A, Lerner G, Brewer ED, Chadha V, Brugnara C, Dahl NV, Watkins SL. Iron therapy in the pediatric hemodialysis population. Pediatr Nephrol. 2004 Jun; 19(6):655-61. View abstract
  108. Brugnara C. Sickle cell disease: from membrane pathophysiology to novel therapies for prevention of erythrocyte dehydration. J Pediatr Hematol Oncol. 2003 Dec; 25(12):927-33. View abstract
  109. Brugnara C. Iron deficiency and erythropoiesis: new diagnostic approaches. Clin Chem. 2003 Oct; 49(10):1573-8. View abstract
  110. Weiner DL, Brugnara C. Hydroxyurea and sickle cell disease: a chance for every patient. JAMA. 2003 Apr 02; 289(13):1692-4. View abstract
  111. Weiner DL, Hibberd PL, Betit P, Cooper AB, Botelho CA, Brugnara C. Preliminary assessment of inhaled nitric oxide for acute vaso-occlusive crisis in pediatric patients with sickle cell disease. JAMA. 2003 Mar 05; 289(9):1136-42. View abstract
  112. Bize I, Taher S, Brugnara C. Regulation of K-Cl cotransport during reticulocyte maturation and erythrocyte aging in normal and sickle erythrocytes. Am J Physiol Cell Physiol. 2003 Jul; 285(1):C31-8. View abstract
  113. Stocker JW, De Franceschi L, McNaughton-Smith GA, Corrocher R, Beuzard Y, Brugnara C. ICA-17043, a novel Gardos channel blocker, prevents sickled red blood cell dehydration in vitro and in vivo in SAD mice. Blood. 2003 Mar 15; 101(6):2412-8. View abstract
  114. Brugnara C. A hematologic "gold standard" for iron-deficient states? Clin Chem. 2002 Jul; 48(7):981-2. View abstract
  115. Khanna R, Chang SH, Andrabi S, Azam M, Kim A, Rivera A, Brugnara C, Low PS, Liu SC, Chishti AH. Headpiece domain of dematin is required for the stability of the erythrocyte membrane. Proc Natl Acad Sci U S A. 2002 May 14; 99(10):6637-42. View abstract
  116. Coffin CM, Hamilton MS, Pysher TJ, Bach P, Ashwood E, Schweiger J, Monahan D, Perry D, Rogers BB, Brugnara C, Rutledge J, Weiss R, Ash O, Hill H, Meikle W, Roberts W, Geaghan S. Pediatric laboratory medicine: current challenges and future opportunities. Am J Clin Pathol. 2002 May; 117(5):683-90. View abstract
  117. Steinberg MH, Brugnara C. Developing treatment for sickle cell disease. Expert Opin Investig Drugs. 2002 May; 11(5):645-59. View abstract
  118. Holm TM, Braun A, Trigatti BL, Brugnara C, Sakamoto M, Krieger M, Andrews NC. Failure of red blood cell maturation in mice with defects in the high-density lipoprotein receptor SR-BI. Blood. 2002 Mar 01; 99(5):1817-24. View abstract
  119. Rivera A, Jarolim P, Brugnara C. Modulation of Gardos channel activity by cytokines in sickle erythrocytes. Blood. 2002 Jan 01; 99(1):357-603. View abstract
  120. Steinberg MH, Brugnara C. Pathophysiological-based approaches to treatment of sickle cell disease. Annu Rev Med. 2003; 54:89-112. View abstract
  121. Socolovsky M, Nam H, Fleming MD, Haase VH, Brugnara C, Lodish HF. Ineffective erythropoiesis in Stat5a(-/-)5b(-/-) mice due to decreased survival of early erythroblasts. Blood. 2001 Dec 01; 98(12):3261-73. View abstract
  122. Brugnara C, De Franceschi L, Beuzard Y. Erythrocyte-active agents and treatment of sickle cell disease. Semin Hematol. 2001 Oct; 38(4):324-32. View abstract
  123. De Franceschi L, Villa-Moruzzi E, Fumagalli L, Brugnara C, Turrini F, Motta R, Veghini E, Corato C, Alper SL, Berton G. K-Cl cotransport modulation by intracellular Mg in erythrocytes from mice bred for low and high Mg levels. Am J Physiol Cell Physiol. 2001 Oct; 281(4):C1385-95. View abstract
  124. Casula S, Shmukler BE, Wilhelm S, Stuart-Tilley AK, Su W, Chernova MN, Brugnara C, Alper SL. A dominant negative mutant of the KCC1 K-Cl cotransporter: both N- and C-terminal cytoplasmic domains are required for K-Cl cotransport activity. J Biol Chem. 2001 Nov 09; 276(45):41870-8. View abstract
  125. Brugnara C, De Franceschi L, Bennekou P, Alper SL, Christophersen P. Novel therapies for prevention of erythrocyte dehydration in sickle cell anemia. Drug News Perspect. 2001 May; 14(4):208-20. View abstract
  126. Bennekou P, de Franceschi L, Pedersen O, Lian L, Asakura T, Evans G, Brugnara C, Christophersen P. Treatment with NS3623, a novel Cl-conductance blocker, ameliorates erythrocyte dehydration in transgenic SAD mice: a possible new therapeutic approach for sickle cell disease. Blood. 2001 Mar 01; 97(5):1451-7. View abstract
  127. Danise P, Amendola G, Nobili B, Perrotta S, Miraglia Del Giudice E, Matarese SM, Iolascon A, Brugnara C. Flow-cytometric analysis of erythrocytes and reticulocytes in congenital dyserythropoietic anaemia type II (CDA II): value in differential diagnosis with hereditary spherocytosis. Clin Lab Haematol. 2001 Feb; 23(1):7-13. View abstract
  128. Brugnara C. Therapeutic strategies for prevention of sickle cell dehydration. Blood Cells Mol Dis. 2001 Jan-Feb; 27(1):71-80. View abstract
  129. Mueller BU, Brugnara C. Prevention of red cell dehydration: a possible new treatment for sickle cell disease. Pediatr Pathol Mol Med. 2001 Jan-Feb; 20(1):15-25. View abstract
  130. Parisotto R, Gore CJ, Hahn AG, Ashenden MJ, Olds TS, Martin DT, Pyne DB, Gawthorn K, Brugnara C. Reticulocyte parameters as potential discriminators of recombinant human erythropoietin abuse in elite athletes. Int J Sports Med. 2000 Oct; 21(7):471-9. View abstract
  131. Bize I, Güvenç B, Buchbinder G, Brugnara C. Stimulation of human erythrocyte K-Cl cotransport and protein phosphatase type 2A by n-ethylmaleimide: role of intracellular Mg++. J Membr Biol. 2000 Sep 15; 177(2):159-68. View abstract
  132. Goodnough LT, Skikne B, Brugnara C. Erythropoietin, iron, and erythropoiesis. Blood. 2000 Aug 01; 96(3):823-33. View abstract
  133. Humbert PO, Rogers C, Ganiatsas S, Landsberg RL, Trimarchi JM, Dandapani S, Brugnara C, Erdman S, Schrenzel M, Bronson RT, Lees JA. E2F4 is essential for normal erythrocyte maturation and neonatal viability. Mol Cell. 2000 Aug; 6(2):281-91. View abstract
  134. Shmukler BE, Brugnara C, Alper SL. Structure and genetic polymorphism of the mouse KCC1 gene. Biochim Biophys Acta. 2000 Jul 24; 1492(2-3):353-61. View abstract
  135. Hansen AR, Lapp C, Brugnara C. Plasminogen activator inhibitor-1: defining characteristics in the cerebrospinal fluid of newborns. J Pediatr. 2000 Jul; 137(1):132-4. View abstract
  136. Parisotto R, Gore CJ, Emslie KR, Ashenden MJ, Brugnara C, Howe C, Martin DT, Trout GJ, Hahn AG. A novel method utilising markers of altered erythropoiesis for the detection of recombinant human erythropoietin abuse in athletes. Haematologica. 2000 Jun; 85(6):564-72. View abstract
  137. Iyer R, Baliga R, Nagel RL, Brugnara C, Kirchner K, Hogan S, Steinberg MH. Maximum urine concentrating ability in children with Hb SC disease: effects of hydroxyurea. Am J Hematol. 2000 May; 64(1):47-52. View abstract
  138. De Franceschi L, Fattovich G, Turrini F, Ayi K, Brugnara C, Manzato F, Noventa F, Stanzial AM, Solero P, Corrocher R. Hemolytic anemia induced by ribavirin therapy in patients with chronic hepatitis C virus infection: role of membrane oxidative damage. Hepatology. 2000 Apr; 31(4):997-1004. View abstract
  139. Brugnara C. Reticulocyte cellular indices: a new approach in the diagnosis of anemias and monitoring of erythropoietic function. Crit Rev Clin Lab Sci. 2000 Apr; 37(2):93-130. View abstract
  140. Donovan A, Brownlie A, Zhou Y, Shepard J, Pratt SJ, Moynihan J, Paw BH, Drejer A, Barut B, Zapata A, Law TC, Brugnara C, Lux SE, Pinkus GS, Pinkus JL, Kingsley PD, Palis J, Fleming MD, Andrews NC, Zon LI. Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter. Nature. 2000 Feb 17; 403(6771):776-81. View abstract
  141. De Franceschi L, Bachir D, Galacteros F, Tchernia G, Cynober T, Neuberg D, Beuzard Y, Brugnara C. Oral magnesium pidolate: effects of long-term administration in patients with sickle cell disease. Br J Haematol. 2000 Feb; 108(2):284-9. View abstract
  142. De Franceschi L, Brugnara C, Rouyer-Fessard P, Jouault H, Beuzard Y. Formation of dense erythrocytes in SAD mice exposed to chronic hypoxia: evaluation of different therapeutic regimens and of a combination of oral clotrimazole and magnesium therapies. Blood. 1999 Dec 15; 94(12):4307-13. View abstract
  143. Su W, Shmukler BE, Chernova MN, Stuart-Tilley AK, de Franceschi L, Brugnara C, Alper SL. Mouse K-Cl cotransporter KCC1: cloning, mapping, pathological expression, and functional regulation. Am J Physiol. 1999 11; 277(5):C899-912. View abstract
  144. Bize I, Güvenç B, Robb A, Buchbinder G, Brugnara C. Serine/threonine protein phosphatases and regulation of K-Cl cotransport in human erythrocytes. Am J Physiol. 1999 11; 277(5):C926-36. View abstract
  145. Rivera A, Rotter MA, Brugnara C. Endothelins activate Ca(2+)-gated K(+) channels via endothelin B receptors in CD-1 mouse erythrocytes. Am J Physiol. 1999 10; 277(4):C746-54. View abstract
  146. Gilligan DM, Lozovatsky L, Gwynn B, Brugnara C, Mohandas N, Peters LL. Targeted disruption of the beta adducin gene (Add2) causes red blood cell spherocytosis in mice. Proc Natl Acad Sci U S A. 1999 Sep 14; 96(19):10717-22. View abstract
  147. Socolovsky M, Fallon AE, Wang S, Brugnara C, Lodish HF. Fetal anemia and apoptosis of red cell progenitors in Stat5a-/-5b-/- mice: a direct role for Stat5 in Bcl-X(L) induction. Cell. 1999 Jul 23; 98(2):181-91. View abstract
  148. Brugnara C, Zurakowski D, DiCanzio J, Boyd T, Platt O. Reticulocyte hemoglobin content to diagnose iron deficiency in children. JAMA. 1999 Jun 16; 281(23):2225-30. View abstract
  149. Peters LL, Jindel HK, Gwynn B, Korsgren C, John KM, Lux SE, Mohandas N, Cohen CM, Cho MR, Golan DE, Brugnara C. Mild spherocytosis and altered red cell ion transport in protein 4. 2-null mice. J Clin Invest. 1999 Jun; 103(11):1527-37. View abstract
  150. Murad KL, Mahany KL, Brugnara C, Kuypers FA, Eaton JW, Scott MD. Structural and functional consequences of antigenic modulation of red blood cells with methoxypoly(ethylene glycol). Blood. 1999 Mar 15; 93(6):2121-7. View abstract
  151. Vitoux D, Beuzard Y, Brugnara C. The effect of hemoglobin A and S on the volume- and pH-dependence of K-Cl cotransport in human erythrocyte ghosts. J Membr Biol. 1999 Feb 01; 167(3):233-40. View abstract
  152. de Franceschi L, Shalev O, Piga A, Collell M, Olivieri O, Corrocher R, Hebbel RP, Brugnara C. Deferiprone therapy in homozygous human beta-thalassemia removes erythrocyte membrane free iron and reduces KCl cotransport activity. J Lab Clin Med. 1999 Jan; 133(1):64-9. View abstract
  153. Tanphaichitr VS, Sumboonnanonda A, Ideguchi H, Shayakul C, Brugnara C, Takao M, Veerakul G, Alper SL. Novel AE1 mutations in recessive distal renal tubular acidosis. Loss-of-function is rescued by glycophorin A. J Clin Invest. 1998 Dec 15; 102(12):2173-9. View abstract
  154. Brownlie A, Donovan A, Pratt SJ, Paw BH, Oates AC, Brugnara C, Witkowska HE, Sassa S, Zon LI. Positional cloning of the zebrafish sauternes gene: a model for congenital sideroblastic anaemia. Nat Genet. 1998 Nov; 20(3):244-50. View abstract
  155. Vandorpe DH, Shmukler BE, Jiang L, Lim B, Maylie J, Adelman JP, de Franceschi L, Cappellini MD, Brugnara C, Alper SL. cDNA cloning and functional characterization of the mouse Ca2+-gated K+ channel, mIK1. Roles in regulatory volume decrease and erythroid differentiation. J Biol Chem. 1998 Aug 21; 273(34):21542-53. View abstract
  156. Jarolim P, Shayakul C, Prabakaran D, Jiang L, Stuart-Tilley A, Rubin HL, Simova S, Zavadil J, Herrin JT, Brouillette J, Somers MJ, Seemanova E, Brugnara C, Guay-Woodford LM, Alper SL. Autosomal dominant distal renal tubular acidosis is associated in three families with heterozygosity for the R589H mutation in the AE1 (band 3) Cl-/HCO3- exchanger. J Biol Chem. 1998 Mar 13; 273(11):6380-8. View abstract
  157. Roback MG, Stack AM, Thompson C, Brugnara C, Schwarz HP, Saladino RA. Activated protein C concentrate for the treatment of meningococcal endotoxin shock in rabbits. Shock. 1998 Feb; 9(2):138-42. View abstract
  158. De Franceschi L, Cappellini MD, Graziadei G, Manzato F, Olivieri O, Corrocher R, Fiorelli G, Beuzard Y, Brugnara C. The effect of dietary magnesium supplementation on the cellular abnormalities of erythrocytes in patients with beta thalassemia intermedia. Haematologica. 1998 Feb; 83(2):118-25. View abstract
  159. Brugnara C. Use of reticulocyte cellular indices in the diagnosis and treatment of hematological disorders. Int J Clin Lab Res. 1998; 28(1):1-11. View abstract
  160. D'onofrio G, Zini G, Brugnara C. Clinical Applications of Automated Reticulocyte Indices. Hematology. 1998; 3(2):165-76. View abstract
  161. Rufo PA, Merlin D, Riegler M, Ferguson-Maltzman MH, Dickinson BL, Brugnara C, Alper SL, Lencer WI. The antifungal antibiotic, clotrimazole, inhibits chloride secretion by human intestinal T84 cells via blockade of distinct basolateral K+ conductances. Demonstration of efficacy in intact rabbit colon and in an in vivo mouse model of cholera. J Clin Invest. 1997 Dec 15; 100(12):3111-20. View abstract
  162. Madore F, Lowrie EG, Brugnara C, Lew NL, Lazarus JM, Bridges K, Owen WF. Anemia in hemodialysis patients: variables affecting this outcome predictor. J Am Soc Nephrol. 1997 Dec; 8(12):1921-9. View abstract
  163. De Franceschi L, Bachir D, Galacteros F, Tchernia G, Cynober T, Alper S, Platt O, Beuzard Y, Brugnara C. Oral magnesium supplements reduce erythrocyte dehydration in patients with sickle cell disease. J Clin Invest. 1997 Oct 01; 100(7):1847-52. View abstract
  164. Browne PV, Shalev O, Kuypers FA, Brugnara C, Solovey A, Mohandas N, Schrier SL, Hebbel RP. Removal of erythrocyte membrane iron in vivo ameliorates the pathobiology of murine thalassemia. J Clin Invest. 1997 Sep 15; 100(6):1459-64. View abstract
  165. Head CA, Brugnara C, Martinez-Ruiz R, Kacmarek RM, Bridges KR, Kuter D, Bloch KD, Zapol WM. Low concentrations of nitric oxide increase oxygen affinity of sickle erythrocytes in vitro and in vivo. J Clin Invest. 1997 Sep 01; 100(5):1193-8. View abstract
  166. Hansen A, Whitelaw A, Lapp C, Brugnara C. Cerebrospinal fluid plasminogen activator inhibitor-1: a prognostic factor in posthaemorrhagic hydrocephalus. Acta Paediatr. 1997 Sep; 86(9):995-8. View abstract
  167. Steinberg MH, Nagel RL, Brugnara C. Cellular effects of hydroxyurea in Hb SC disease. Br J Haematol. 1997 Sep; 98(4):838-44. View abstract
  168. Major A, Mathez-Loic F, Rohling R, Gautschi K, Brugnara C. The effect of intravenous iron on the reticulocyte response to recombinant human erythropoietin. Br J Haematol. 1997 Aug; 98(2):292-4. View abstract
  169. De Franceschi L, Brugnara C, Beuzard Y. Dietary magnesium supplementation ameliorates anemia in a mouse model of beta-thalassemia. Blood. 1997 Aug 01; 90(3):1283-90. View abstract
  170. Brugnara C, Zelmanovic D, Sorette M, Ballas SK, Platt O. Reticulocyte hemoglobin: an integrated parameter for evaluation of erythropoietic activity. Am J Clin Pathol. 1997 Aug; 108(2):133-42. View abstract
  171. Rittenhouse AR, Parker C, Brugnara C, Morgan KG, Alper SL. Inhibition of maxi-K currents in ferret portal vein smooth muscle cells by the antifungal clotrimazole. Am J Physiol. 1997 Jul; 273(1 Pt 1):C45-56. View abstract
  172. Cazzola M, Mercuriali F, Brugnara C. Use of recombinant human erythropoietin outside the setting of uremia. Blood. 1997 Jun 15; 89(12):4248-67. View abstract
  173. Rittenhouse AR, Vandorpe DH, Brugnara C, Alper SL. The antifungal imidazole clotrimazole and its major in vivo metabolite are potent blockers of the calcium-activated potassium channel in murine erythroleukemia cells. J Membr Biol. 1997 May 15; 157(2):177-91. View abstract
  174. Magnani B, Fenton T, Lapp C, Brugnara C. Degree of agreement in plasma fibrinogen among two functional and one immunonephelometric assays. Am J Clin Pathol. 1997 May; 107(5):527-33. View abstract
  175. Brugnara C. Erythrocyte membrane transport physiology. Curr Opin Hematol. 1997 Mar; 4(2):122-7. View abstract
  176. Chan FY, Robinson J, Brownlie A, Shivdasani RA, Donovan A, Brugnara C, Kim J, Lau BC, Witkowska HE, Zon LI. Characterization of adult alpha- and beta-globin genes in the zebrafish. Blood. 1997 Jan 15; 89(2):688-700. View abstract
  177. Shmukler B, Sun T, Brugnara C, Alper SL. Reinterpretation of the RACTK1 K+ channel. Am J Physiol. 1997 Jan; 272(1 Pt 1):C350-4. View abstract
  178. Bridges KR, Barabino GD, Brugnara C, Cho MR, Christoph GW, Dover G, Ewenstein BM, Golan DE, Guttmann CR, Hofrichter J, Mulkern RV, Zhang B, Eaton WA. A multiparameter analysis of sickle erythrocytes in patients undergoing hydroxyurea therapy. Blood. 1996 Dec 15; 88(12):4701-10. View abstract
  179. Rufo PA, Jiang L, Moe SJ, Brugnara C, Alper SL, Lencer WI. The antifungal antibiotic, clotrimazole, inhibits Cl- secretion by polarized monolayers of human colonic epithelial cells. J Clin Invest. 1996 Nov 01; 98(9):2066-75. View abstract
  180. De Franceschi L, Beuzard Y, Jouault H, Brugnara C. Modulation of erythrocyte potassium chloride cotransport, potassium content, and density by dietary magnesium intake in transgenic SAD mouse. Blood. 1996 Oct 01; 88(7):2738-44. View abstract
  181. Peters LL, Shivdasani RA, Liu SC, Hanspal M, John KM, Gonzalez JM, Brugnara C, Gwynn B, Mohandas N, Alper SL, Orkin SH, Lux SE. Anion exchanger 1 (band 3) is required to prevent erythrocyte membrane surface loss but not to form the membrane skeleton. Cell. 1996 Sep 20; 86(6):917-27. View abstract
  182. Brugnara C, Gee B, Armsby CC, Kurth S, Sakamoto M, Rifai N, Alper SL, Platt OS. Therapy with oral clotrimazole induces inhibition of the Gardos channel and reduction of erythrocyte dehydration in patients with sickle cell disease. J Clin Invest. 1996 Mar 01; 97(5):1227-34. View abstract
  183. Armsby CC, Stuart-Tilley AK, Alper SL, Brugnara C. Resistance to osmotic lysis in BXD-31 mouse erythrocytes: association with upregulated K-Cl cotransport. Am J Physiol. 1996 Mar; 270(3 Pt 1):C866-77. View abstract
  184. de Franceschi L, Rouyer-Fessard P, Alper SL, Jouault H, Brugnara C, Beuzard Y. Combination therapy of erythropoietin, hydroxyurea, and clotrimazole in a beta thalassemic mouse: a model for human therapy. Blood. 1996 Feb 01; 87(3):1188-95. View abstract
  185. Coupry I, Armsby CC, Alper SL, Brugnara C, Parini A. Clotrimazole and efaroxan inhibit red cell Gardos channel independently of imidazoline I1 and I2 binding sites. Eur J Pharmacol. 1996 Jan 04; 295(1):109-12. View abstract
  186. De Franceschi L, Beuzard Y, Brugnara C. Sulfhydryl oxidation and activation of red cell K(+)-Cl- cotransport in the transgenic SAD mouse. Am J Physiol. 1995 Oct; 269(4 Pt 1):C899-906. View abstract
  187. Rifai N, Sakamoto M, Platt O, Brugnara C. A high-performance liquid chromatographic assay for the determination of itraconazole concentration using solid-phase extraction and small sample volume. Ther Drug Monit. 1995 Oct; 17(5):522-5. View abstract
  188. Brugnara C, Armsby CC, De Franceschi L, Crest M, Euclaire MF, Alper SL. Ca(2+)-activated K+ channels of human and rabbit erythrocytes display distinctive patterns of inhibition by venom peptide toxins. J Membr Biol. 1995 Sep; 147(1):71-82. View abstract
  189. Coupry I, Armsby CC, Alper S, Parini A, Brugnara C. Inhibition of red cell Ca(2+)-activated K+ transport by clotrimazole does not take place via imidazoline binding sites. Ann N Y Acad Sci. 1995 Jul 12; 763:287-9. View abstract
  190. Brugnara C, De Franceschi L, Armsby CC, Saadane N, Trudel M, Beuzard Y, Rittenhouse A, Rifai N, Platt O, Alper SL. A new therapeutic approach for sickle cell disease. Blockade of the red cell Ca(2+)-activated K+ channel by clotrimazole. Ann N Y Acad Sci. 1995 Jul 12; 763:262-71. View abstract
  191. Benzaquen LR, Brugnara C, Byers HR, Gatton-Celli S, Halperin JA. Clotrimazole inhibits cell proliferation in vitro and in vivo. Nat Med. 1995 Jun; 1(6):534-40. View abstract
  192. Brugnara C, Armsby CC, Sakamoto M, Rifai N, Alper SL, Platt O. Oral administration of clotrimazole and blockade of human erythrocyte Ca(++)-activated K+ channel: the imidazole ring is not required for inhibitory activity. J Pharmacol Exp Ther. 1995 Apr; 273(1):266-72. View abstract
  193. Armsby CC, Brugnara C, Alper SL. Cation transport in mouse erythrocytes: role of K(+)-Cl- cotransport in regulatory volume decrease. Am J Physiol. 1995 Apr; 268(4 Pt 1):C894-902. View abstract
  194. Rifai N, Sakamoto M, Law T, Platt O, Mikati M, Armsby CC, Brugnara C. HPLC measurement, blood distribution, and pharmacokinetics of oral clotrimazole, potentially useful antisickling agent. Clin Chem. 1995 Mar; 41(3):387-91. View abstract
  195. Brugnara C. Erythrocyte dehydration in pathophysiology and treatment of sickle cell disease. Curr Opin Hematol. 1995 Mar; 2(2):132-8. View abstract
  196. Jarolim P, Rubin HL, Brabec V, Chrobak L, Zolotarev AS, Alper SL, Brugnara C, Wichterle H, Palek J. Mutations of conserved arginines in the membrane domain of erythroid band 3 lead to a decrease in membrane-associated band 3 and to the phenotype of hereditary spherocytosis. Blood. 1995 Feb 01; 85(3):634-40. View abstract
  197. Brugnara C, Hipp MJ, Irving PJ, Lathrop H, Lee PA, Minchello EM, Winkelman J. Automated reticulocyte counting and measurement of reticulocyte cellular indices. Evaluation of the Miles H*3 blood analyzer. Am J Clin Pathol. 1994 Nov; 102(5):623-32. View abstract
  198. Olivieri O, De Franceschi L, Capellini MD, Girelli D, Corrocher R, Brugnara C. Oxidative damage and erythrocyte membrane transport abnormalities in thalassemias. Blood. 1994 Jul 01; 84(1):315-20. View abstract
  199. Brugnara C, Laufer MR, Friedman AJ, Bridges K, Platt O. Reticulocyte hemoglobin content (CHr): early indicator of iron deficiency and response to therapy. Blood. 1994 May 15; 83(10):3100-1. View abstract
  200. Brugnara C, Colella GM, Cremins J, Langley RC, Schneider TJ, Rutherford CJ, Goldberg MA. Effects of subcutaneous recombinant human erythropoietin in normal subjects: development of decreased reticulocyte hemoglobin content and iron-deficient erythropoiesis. J Lab Clin Med. 1994 May; 123(5):660-7. View abstract
  201. Brugnara C, Fenton T, Winkelman JW. Management training for pathology residents. I. Results of a national survey. Am J Clin Pathol. 1994 May; 101(5):559-63. View abstract
  202. Winkelman JW, Brugnara C. Management training for pathology residents. II. Experience with a focused curriculum. Am J Clin Pathol. 1994 May; 101(5):564-8. View abstract
  203. De Franceschi L, Saadane N, Trudel M, Alper SL, Brugnara C, Beuzard Y. Treatment with oral clotrimazole blocks Ca(2+)-activated K+ transport and reverses erythrocyte dehydration in transgenic SAD mice. A model for therapy of sickle cell disease. J Clin Invest. 1994 Apr; 93(4):1670-6. View abstract
  204. Rutherford CJ, Schneider TJ, Dempsey H, Kirn DH, Brugnara C, Goldberg MA. Efficacy of different dosing regimens for recombinant human erythropoietin in a simulated perisurgical setting: the importance of iron availability in optimizing response. Am J Med. 1994 Feb; 96(2):139-45. View abstract
  205. Jarolim P, Rubin HL, Liu SC, Cho MR, Brabec V, Derick LH, Yi SJ, Saad ST, Alper S, Brugnara C, et al. Duplication of 10 nucleotides in the erythroid band 3 (AE1) gene in a kindred with hereditary spherocytosis and band 3 protein deficiency (band 3PRAGUE). J Clin Invest. 1994 Jan; 93(1):121-30. View abstract
  206. Brugnara C, de Franceschi L, Alper SL. Inhibition of Ca(2+)-dependent K+ transport and cell dehydration in sickle erythrocytes by clotrimazole and other imidazole derivatives. J Clin Invest. 1993 Jul; 92(1):520-6. View abstract
  207. Brugnara C, Kruskall MS, Johnstone RM. Membrane properties of erythrocytes in subjects undergoing multiple blood donations with or without recombinant erythropoietin. Br J Haematol. 1993 May; 84(1):118-30. View abstract
  208. Brugnara C, De Franceschi L, Alper SL. Ca(2+)-activated K+ transport in erythrocytes. Comparison of binding and transport inhibition by scorpion toxins. J Biol Chem. 1993 Apr 25; 268(12):8760-8. View abstract
  209. Brugnara C. Membrane transport of Na and K and cell dehydration in sickle erythrocytes. Experientia. 1993 Feb 15; 49(2):100-9. View abstract
  210. Brugnara C, Chambers LA, Malynn E, Goldberg MA, Kruskall MS. Red blood cell regeneration induced by subcutaneous recombinant erythropoietin: iron-deficient erythropoiesis in iron-replete subjects. Blood. 1993 Feb 15; 81(4):956-64. View abstract
  211. Brugnara C, de Franceschi L. Effect of cell age and phenylhydrazine on the cation transport properties of rabbit erythrocytes. J Cell Physiol. 1993 Feb; 154(2):271-80. View abstract
  212. Goldberg MA, Brugnara C, Dover GJ, Schapira L, Lacroix L, Bunn HF. Hydroxyurea and erythropoietin therapy in sickle cell anemia. Semin Oncol. 1992 Jun; 19(3 Suppl 9):74-81. View abstract
  213. Brugnara C, Churchill WH. Effect of irradiation on red cell cation content and transport. Transfusion. 1992 Mar-Apr; 32(3):246-52. View abstract
  214. Churchill WH, Schmidt B, Lindsey J, Greenberg M, Boudrow S, Brugnara C. Thawing fresh frozen plasma in a microwave oven. A comparison with thawing in a 37 degrees C waterbath. Am J Clin Pathol. 1992 Feb; 97(2):227-32. View abstract
  215. Olivieri O, Vitoux D, Galacteros F, Bachir D, Blouquit Y, Beuzard Y, Brugnara C. Hemoglobin variants and activity of the (K+Cl-) cotransport system in human erythrocytes. Blood. 1992 Feb 01; 79(3):793-7. View abstract
  216. Brugnara C. [Physiopathology of erythrocyte membrane transport]. Haematologica. 1991 Jun; 76 Suppl 3:191-8. View abstract
  217. Goldberg MA, Brugnara C, Dover GJ, Schapira L, Charache S, Bunn HF. Treatment of sickle cell anemia with hydroxyurea and erythropoietin. N Engl J Med. 1990 Aug 09; 323(6):366-72. View abstract
  218. Halperin JA, Brugnara C, Van Ha T, Tosteson DC. Voltage-activated cation permeability in high-potassium but not low-potassium red blood cells. Am J Physiol. 1990 Jun; 258(6 Pt 1):C1169-72. View abstract
  219. Halperin JA, Brugnara C, Tosteson MT, Van Ha T, Tosteson DC. Voltage-activated cation transport in human erythrocytes. Am J Physiol. 1989 Nov; 257(5 Pt 1):C986-96. View abstract
  220. Brugnara C. Characteristics of the volume- and chloride-dependent K transport in human erythrocytes homozygous for hemoglobin C. J Membr Biol. 1989 Oct; 111(1):69-81. View abstract
  221. Brugnara C, Van Ha T, Tosteson DC. Acid pH induces formation of dense cells in sickle erythrocytes. Blood. 1989 Jul; 74(1):487-95. View abstract
  222. Brugnara C, Van Ha T, Tosteson DC. Role of chloride in potassium transport through a K-Cl cotransport system in human red blood cells. Am J Physiol. 1989 May; 256(5 Pt 1):C994-1003. View abstract
  223. Halperin JA, Brugnara C, Nicholson-Weller A. Ca2+-activated K+ efflux limits complement-mediated lysis of human erythrocytes. J Clin Invest. 1989 May; 83(5):1466-71. View abstract
  224. Brugnara C, Bunn HF, Tosteson DC. Ion content and transport and the regulation of volume in sickle cells. Ann N Y Acad Sci. 1989; 565:96-103. View abstract
  225. Brugnara C, Van Ha T, Tosteson DC. Properties of K+ transport in resealed human erythrocyte ghosts. Am J Physiol. 1988 Sep; 255(3 Pt 1):C346-56. View abstract
  226. Halperin JA, Nicholson-Weller A, Brugnara C, Tosteson DC. Complement induces a transient increase in membrane permeability in unlysed erythrocytes. J Clin Invest. 1988 Aug; 82(2):594-600. View abstract
  227. Brugnara C, Tosteson DC. Inhibition of K transport by divalent cations in sickle erythrocytes. Blood. 1987 Dec; 70(6):1810-5. View abstract
  228. Canessa M, Brugnara C, Escobales N. The Li+-Na+ exchange and Na+-K+-Cl- cotransport systems in essential hypertension. Hypertension. 1987 Nov; 10(5 Pt 2):I4-10. View abstract
  229. Corrocher R, Ferrari S, Bassi A, Guarini P, Bertinato L, Olivieri O, Licia GM, Ruzzenente O, Brugnara C, De Sandre G. Membrane polyunsaturated fatty acids and lithium-sodium countertransport in human erythrocytes. Life Sci. 1987 Aug 31; 41(9):1171-8. View abstract
  230. Halperin JA, Brugnara C, Kopin AS, Ingwall J, Tosteson DC. Properties of the Na+-K+ pump in human red cells with increased number of pump sites. J Clin Invest. 1987 Jul; 80(1):128-37. View abstract
  231. Brugnara C, Tosteson DC. Cell volume, K transport, and cell density in human erythrocytes. Am J Physiol. 1987 Mar; 252(3 Pt 1):C269-76. View abstract
  232. Brock TA, Brugnara C, Canessa M, Gimbrone MA. Bradykinin and vasopressin stimulate Na+-K+-Cl- cotransport in cultured endothelial cells. Am J Physiol. 1986 Jun; 250(6 Pt 1):C888-95. View abstract
  233. Brugnara C, Bunn HF, Tosteson DC. Regulation of erythrocyte cation and water content in sickle cell anemia. Science. 1986 Apr 18; 232(4748):388-90. View abstract
  234. Corrocher R, Bonfanti F, Steinmayr M, Brugnara C, Bertinato L, De Sandre G. [Frequency and sex and age distribution of cardiopathy in arterial hypertension]. Minerva Cardioangiol. 1986 Mar; 34(3):119-25. View abstract
  235. Bonfanti F, Corrocher R, Bertinato L, Brugnara C, Nortilli R, De Sandre G. [Incidence of retinal and renal complications in arterial hypertension]. Minerva Cardioangiol. 1986 Mar; 34(3):99-105. View abstract
  236. Canessa M, Brugnara C, Cusi D, Tosteson DC. Modes of operation and variable stoichiometry of the furosemide- sensitive Na and K fluxes in human red cells. J Gen Physiol. 1986 Jan; 87(1):113-42. View abstract
  237. Brugnara C, Canessa M, Cusi D, Tosteson DC. Furosemide-sensitive Na and K fluxes in human red cells. Net uphill Na extrusion and equilibrium properties. J Gen Physiol. 1986 Jan; 87(1):91-112. View abstract
  238. Brugnara C, Kopin AS, Bunn HF, Tosteson DC. Regulation of cation content and cell volume in hemoglobin erythrocytes from patients with homozygous hemoglobin C disease. J Clin Invest. 1985 May; 75(5):1608-17. View abstract
  239. Corrocher R, Steinmayr M, Ruzzenente O, Brugnara C, Bertinato L, Mazzi M, Furri C, Bonfanti F, De Sandre G. Elevation of red cell sodium-lithium countertransport in hyperlipidemias. Life Sci. 1985 Feb 18; 36(7):649-55. View abstract
  240. Canessa M, Brugnara C. The regulation of Na-K cotransport system and essential hypertension. Klin Wochenschr. 1985; 63 Suppl 3:52-4. View abstract
  241. Dagher G, Brugnara C, Canessa M. Effect of metabolic depletion on the furosemide-sensitive Na and K fluxes in human red cells. J Membr Biol. 1985; 86(2):145-55. View abstract
  242. Corrocher R, Brugnara C, Maso R, Taddei G, Mezzelani P, De Sandre G. Multiple arterial stenoses in chronic ergot toxicity. N Engl J Med. 1984 Jan 26; 310(4):261. View abstract
  243. Brugnara C, Kopin AS, Bunn HF, Tosteson DC. Electrolyte composition and equilibrium in hemoglobin CC red blood cells. Trans Assoc Am Physicians. 1984; 97:104-12. View abstract
  244. Brugnara C, Corrocher R, Foroni L, Steinmayr M, Bonfanti F, De Sandre G. Lithium-sodium countertransport in erythrocytes of normal and hypertensive subjects. Relationship with age and plasma renin activity. Hypertension. 1983 Jul-Aug; 5(4):529-34. View abstract
  245. Bonfanti F, Pecci R, Nortilli R, Brugnara C, Corrocher R. [Relations between renin activity and vascular complications of arterial hypertension]. Minerva Cardioangiol. 1982 Mar; 30(3):75-80. View abstract
  246. Bonfanti F, Corrocher R, Cherubini M, Brugnara C, Fugazzola C, Frasson F. [Multiple renal arteries and arterial hypertension: a review of 337 renal arteriography cases]. Minerva Cardioangiol. 1980 May; 28(5):289-04. View abstract