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Jordan Kreidberg, MD, PhD

John Kreidberg, MD, PhD
Urology Research
Hospital Title:
Director, Development and Stem Cell Research
Academic Title:
Professor of Pediatrics, Harvard Medical School
Research Focus Area:
Stem cells for kidney failure
Contact Via Email
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Research Overview

Jordan Kreidberg's principal research goal is to understand the genetic mechanisms of organogenesis, with a focus on the urogenital system. More specifically, his lab seeks to:
  • Understand the inductive mechanisms of early organ development
  • Define regulatory interactions that establish and maintain normal glomerular function and prevent chronic renal failure
  • Define the stem cell compartment of the developing kidney, and develop approaches for propagating the stem cell compartment

If kidney stem cells can be defined and propagated, they may ultimately be used to regenerate new kidney tissue in individuals with chronic renal failure. Determining the regulatory interactions within the glomerulus could also yield treatments to reverse glomerular damage and prevent situations that lead to nephrotic syndrome and chronic renal failure.

The researchers have recently demonstrated a novel inductive interaction between angioblasts--the cells that give rise to blood vessels--and mesenchymal precursor cells--which differentiate into connective tissue--that is required for early kidney development. They are also determining how the integrin family of adhesion receptors participate in organogenesis and are examining how the Wt1 transcription factor and the alpha3 beta1 integrin receptor regulate glomerular development and maintain normal glomerular function.

About Jordan Kreidberg

Dr. Kreidberg received his MD and PhD from Johns Hopkins University School of Medicine. He completed his internship and at Boston Children's Hospital Boston and a postdoctoral fellowship at the Whitehead Institute.


Publications powered by Harvard Catalyst Profiles
  1. Kann M, Bae E, Lenz MO, Li L, Trannguyen B, Schumacher VA, Taglienti ME, Bordeianou L, Hartwig S, Rinschen MM, Schermer B, Benzing T, Fan CM, Kreidberg JA. WT1 targets Gas1 to maintain nephron progenitor cells by modulating FGF signals. Development. 2015 Apr 1; 142(7):1254-66.
  2. Kann M, Ettou S, Jung YL, Lenz MO, Taglienti ME, Park PJ, Schermer B, Benzing T, Kreidberg JA. Genome-Wide Analysis of Wilms' Tumor 1-Controlled Gene Expression in Podocytes Reveals Key Regulatory Mechanisms. J Am Soc Nephrol. 2015 Sep; 26(9):2097-104.
  3. Zhang AF, Wu SL, Jung Y, Qin S, Hancock WS, Kreidberg JA. Identification of novel glycans with disialylated structures in a3 integrin from mouse kidney cells with the phenotype of polycystic kidney disease. J Proteome Res. 2014 Nov 7; 13(11):4901-9.
  4. Chu JY, Sims-Lucas S, Bushnell DS, Bodnar AJ, Kreidberg JA, Ho J. Dicer function is required in the metanephric mesenchyme for early kidney development. Am J Physiol Renal Physiol. 2014 Apr 1; 306(7):F764-72.
  5. Kreidberg J. Introduction to the 12th International Workshop on Developmental Nephrology. Pediatr Nephrol. 2014 Apr; 29(4):497-8.
  6. Pellegatta M, De Arcangelis A, D'Urso A, Nodari A, Zambroni D, Ghidinelli M, Matafora V, Williamson C, Georges-Labouesse E, Kreidberg J, Mayer U, McKee KK, Yurchenco PD, Quattrini A, Wrabetz L, Feltri ML. a6ß1 and a7ß1 integrins are required in Schwann cells to sort axons. J Neurosci. 2013 Nov 13; 33(46):17995-8007.
  7. Tomita M, Asada M, Asada N, Nakamura J, Oguchi A, Higashi AY, Endo S, Robertson E, Kimura T, Kita T, Economides AN, Kreidberg J, Yanagita M. Bmp7 maintains undifferentiated kidney progenitor population and determines nephron numbers at birth. PLoS One. 2013; 8(8):e73554.
  8. Jeong SJ, Luo R, Singer K, Giera S, Kreidberg J, Kiyozumi D, Shimono C, Sekiguchi K, Piao X. GPR56 functions together with a3ß1 integrin in regulating cerebral cortical development. PLoS One. 2013; 8(7):e68781.
  9. McMahon GM, Datta D, Bruneau S, Kann M, Khalid M, Ho J, Seto T, Kreidberg JA, Stillman IE, Briscoe DM. Constitutive activation of the mTOR signaling pathway within the normal glomerulus. Biochem Biophys Res Commun. 2012 Aug 24; 425(2):244-9.
  10. Qin S, Taglienti M, Cai L, Zhou J, Kreidberg JA. c-Met and NF-?B-dependent overexpression of Wnt7a and -7b and Pax2 promotes cystogenesis in polycystic kidney disease. J Am Soc Nephrol. 2012 Aug; 23(8):1309-18.
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  12. Ho J, Kreidberg JA. MicroRNAs in renal development. Pediatr Nephrol. 2013 Feb; 28(2):219-25.
  13. Ho J, Kreidberg JA. The long and short of microRNAs in the kidney. J Am Soc Nephrol. 2012 Mar; 23(3):400-4.
  14. Schumacher VA, Schlötzer-Schrehardt U, Karumanchi SA, Shi X, Zaia J, Jeruschke S, Zhang D, Pavenstädt H, Pavenstaedt H, Drenckhan A, Amann K, Ng C, Hartwig S, Ng KH, Ho J, Kreidberg JA, Taglienti M, Royer-Pokora B, Ai X. WT1-dependent sulfatase expression maintains the normal glomerular filtration barrier. J Am Soc Nephrol. 2011 Jul; 22(7):1286-96.
  15. Ho J, Pandey P, Schatton T, Sims-Lucas S, Khalid M, Frank MH, Hartwig S, Kreidberg JA. The pro-apoptotic protein Bim is a microRNA target in kidney progenitors. J Am Soc Nephrol. 2011 Jun; 22(6):1053-63.
  16. Pandey P, Qin S, Ho J, Zhou J, Kreidberg JA. Systems biology approach to identify transcriptome reprogramming and candidate microRNA targets during the progression of polycystic kidney disease. BMC Syst Biol. 2011; 5:56.
  17. Jarad G, Pippin JW, Shankland SJ, Kreidberg JA, Miner JH. Dystroglycan does not contribute significantly to kidney development or function, in health or after injury. Am J Physiol Renal Physiol. 2011 Mar; 300(3):F811-20.
  18. Langsdorf A, Schumacher V, Shi X, Tran T, Zaia J, Jain S, Taglienti M, Kreidberg JA, Fine A, Ai X. Expression regulation and function of heparan sulfate 6-O-endosulfatases in the spermatogonial stem cell niche. Glycobiology. 2011 Feb; 21(2):152-61.
  19. Qin S, Taglienti M, Nauli SM, Contrino L, Takakura A, Zhou J, Kreidberg JA. Failure to ubiquitinate c-Met leads to hyperactivation of mTOR signaling in a mouse model of autosomal dominant polycystic kidney disease. J Clin Invest. 2010 Oct; 120(10):3617-28.
  20. da Silva RG, Tavora B, Robinson SD, Reynolds LE, Szekeres C, Lamar J, Batista S, Kostourou V, Germain MA, Reynolds AR, Jones DT, Watson AR, Jones JL, Harris A, Hart IR, Iruela-Arispe ML, Dipersio CM, Kreidberg JA, Hodivala-Dilke KM. Endothelial alpha3beta1-integrin represses pathological angiogenesis and sustains endothelial-VEGF. Am J Pathol. 2010 Sep; 177(3):1534-48.
  21. Kreidberg JA. WT1 and kidney progenitor cells. Organogenesis. 2010 Apr-Jun; 6(2):61-70.
  22. Hartwig S, Ho J, Pandey P, Macisaac K, Taglienti M, Xiang M, Alterovitz G, Ramoni M, Fraenkel E, Kreidberg JA. Genomic characterization of Wilms' tumor suppressor 1 targets in nephron progenitor cells during kidney development. Development. 2010 Apr; 137(7):1189-203.
  23. Kreidberg JA. siRNA therapy for glomerulonephritis. J Am Soc Nephrol. 2010 Apr; 21(4):549-51.
  24. Kong T, Xu D, Yu W, Takakura A, Boucher I, Tran M, Kreidberg JA, Shah J, Zhou J, Denker BM. G alpha 12 inhibits alpha2 beta1 integrin-mediated Madin-Darby canine kidney cell attachment and migration on collagen-I and blocks tubulogenesis. Mol Biol Cell. 2009 Nov; 20(21):4596-610.
  25. Mitchell K, Szekeres C, Milano V, Svenson KB, Nilsen-Hamilton M, Kreidberg JA, DiPersio CM. Alpha3beta1 integrin in epidermis promotes wound angiogenesis and keratinocyte-to-endothelial-cell crosstalk through the induction of MRP3. J Cell Sci. 2009 Jun 1; 122(Pt 11):1778-87.
  26. Stanco A, Szekeres C, Patel N, Rao S, Campbell K, Kreidberg JA, Polleux F, Anton ES. Netrin-1-alpha3beta1 integrin interactions regulate the migration of interneurons through the cortical marginal zone. Proc Natl Acad Sci U S A. 2009 May 5; 106(18):7595-600.
  27. Liu Y, Chattopadhyay N, Qin S, Szekeres C, Vasylyeva T, Mahoney ZX, Taglienti M, Bates CM, Chapman HA, Miner JH, Kreidberg JA. Coordinate integrin and c-Met signaling regulate Wnt gene expression during epithelial morphogenesis. Development. 2009 Mar; 136(5):843-53.
  28. Kim KK, Wei Y, Szekeres C, Kugler MC, Wolters PJ, Hill ML, Frank JA, Brumwell AN, Wheeler SE, Kreidberg JA, Chapman HA. Epithelial cell alpha3beta1 integrin links beta-catenin and Smad signaling to promote myofibroblast formation and pulmonary fibrosis. J Clin Invest. 2009 Jan; 119(1):213-24.
  29. Walker JL, Menko AS, Khalil S, Rebustini I, Hoffman MP, Kreidberg JA, Kukuruzinska MA. Diverse roles of E-cadherin in the morphogenesis of the submandibular gland: insights into the formation of acinar and ductal structures. Dev Dyn. 2008 Nov; 237(11):3128-41.
  30. Kazama I, Mahoney Z, Miner JH, Graf D, Economides AN, Kreidberg JA. Podocyte-derived BMP7 is critical for nephron development. J Am Soc Nephrol. 2008 Nov; 19(11):2181-91.
  31. Ho J, Ng KH, Rosen S, Dostal A, Gregory RI, Kreidberg JA. Podocyte-specific loss of functional microRNAs leads to rapid glomerular and tubular injury. J Am Soc Nephrol. 2008 Nov; 19(11):2069-75.
  32. Kreidberg JA, Hartwig S. Wilms' tumor-1: a riddle wrapped in a mystery, inside a kidney. Kidney Int. 2008 Aug; 74(4):411-2.
  33. Kreidberg JA. Integrins and matrix in the glomerulus: old mysteries and new insights. J Am Soc Nephrol. 2008 Apr; 19(4):650-1.
  34. Quaggin SE, Kreidberg JA. Development of the renal glomerulus: good neighbors and good fences. Development. 2008 Feb; 135(4):609-20.
  35. Matilainen T, Haugas M, Kreidberg JA, Salminen M. Analysis of Netrin 1 receptors during inner ear development. Int J Dev Biol. 2007; 51(5):409-13.
  36. Gao X, Chen X, Taglienti M, Rumballe B, Little MH, Kreidberg JA. Angioblast-mesenchyme induction of early kidney development is mediated by Wt1 and Vegfa. Development. 2005 Dec; 132(24):5437-49.
  37. Schmid RS, Jo R, Shelton S, Kreidberg JA, Anton ES. Reelin, integrin and DAB1 interactions during embryonic cerebral cortical development. Cereb Cortex. 2005 Oct; 15(10):1632-6.
  38. Schmid RS, Shelton S, Stanco A, Yokota Y, Kreidberg JA, Anton ES. alpha3beta1 integrin modulates neuronal migration and placement during early stages of cerebral cortical development. Development. 2004 Dec; 131(24):6023-31.
  39. Reiser J, Oh J, Shirato I, Asanuma K, Hug A, Mundel TM, Honey K, Ishidoh K, Kominami E, Kreidberg JA, Tomino Y, Mundel P. Podocyte migration during nephrotic syndrome requires a coordinated interplay between cathepsin L and alpha3 integrin. J Biol Chem. 2004 Aug 13; 279(33):34827-32.
  40. Chen D, Roberts R, Pohl M, Nigam S, Kreidberg J, Wang Z, Heino J, Ivaska J, Coffa S, Harris RC, Pozzi A, Zent R. Differential expression of collagen- and laminin-binding integrins mediates ureteric bud and inner medullary collecting duct cell tubulogenesis. Am J Physiol Renal Physiol. 2004 Oct; 287(4):F602-11.
  41. Reiser J, von Gersdorff G, Loos M, Oh J, Asanuma K, Giardino L, Rastaldi MP, Calvaresi N, Watanabe H, Schwarz K, Faul C, Kretzler M, Davidson A, Sugimoto H, Kalluri R, Sharpe AH, Kreidberg JA, Mundel P. Induction of B7-1 in podocytes is associated with nephrotic syndrome. J Clin Invest. 2004 May; 113(10):1390-7.
  42. Natoli TA, Alberta JA, Bortvin A, Taglienti ME, Menke DB, Loring J, Jaenisch R, Page DC, Housman DE, Kreidberg JA. Wt1 functions in the development of germ cells in addition to somatic cell lineages of the testis. Dev Biol. 2004 Apr 15; 268(2):429-40.
  43. Chattopadhyay N, Wang Z, Ashman LK, Brady-Kalnay SM, Kreidberg JA. alpha3beta1 integrin-CD151, a component of the cadherin-catenin complex, regulates PTPmu expression and cell-cell adhesion. J Cell Biol. 2003 Dec 22; 163(6):1351-62.
  44. Zhang F, Tom CC, Kugler MC, Ching TT, Kreidberg JA, Wei Y, Chapman HA. Distinct ligand binding sites in integrin alpha3beta1 regulate matrix adhesion and cell-cell contact. J Cell Biol. 2003 Oct 13; 163(1):177-88.
  45. Zhang H, Palmer R, Gao X, Kreidberg J, Gerald W, Hsiao L, Jensen RV, Gullans SR, Haber DA. Transcriptional activation of placental growth factor by the forkhead/winged helix transcription factor FoxD1. Curr Biol. 2003 Sep 16; 13(18):1625-9.
  46. Kreidberg JA. Podocyte differentiation and glomerulogenesis. J Am Soc Nephrol. 2003 Mar; 14(3):806-14.
  47. He ZY, Brakebusch C, Fässler R, Kreidberg JA, Primakoff P, Myles DG. None of the integrins known to be present on the mouse egg or to be ADAM receptors are essential for sperm-egg binding and fusion. Dev Biol. 2003 Feb 15; 254(2):226-37.
  48. Alberta JA, Springett GM, Rayburn H, Natoli TA, Loring J, Kreidberg JA, Housman D. Role of the WT1 tumor suppressor in murine hematopoiesis. Blood. 2003 Apr 1; 101(7):2570-4.
  49. Natoli TA, Liu J, Eremina V, Hodgens K, Li C, Hamano Y, Mundel P, Kalluri R, Miner JH, Quaggin SE, Kreidberg JA. A mutant form of the Wilms' tumor suppressor gene WT1 observed in Denys-Drash syndrome interferes with glomerular capillary development. J Am Soc Nephrol. 2002 Aug; 13(8):2058-67.
  50. Menko AS, Zhang L, Schiano F, Kreidberg JA, Kukuruzinska MA. Regulation of cadherin junctions during mouse submandibular gland development. Dev Dyn. 2002 Jul; 224(3):321-33.
  51. Natoli TA, McDonald A, Alberta JA, Taglienti ME, Housman DE, Kreidberg JA. A mammal-specific exon of WT1 is not required for development or fertility. Mol Cell Biol. 2002 Jun; 22(12):4433-8.
  52. Yang X, Claas C, Kraeft SK, Chen LB, Wang Z, Kreidberg JA, Hemler ME. Palmitoylation of tetraspanin proteins: modulation of CD151 lateral interactions, subcellular distribution, and integrin-dependent cell morphology. Mol Biol Cell. 2002 Mar; 13(3):767-81.
  53. Kreidberg J. Kidneys and sex, the Wilms' tumor connection. Pediatr Res. 2002 Feb; 51(2):128.
  54. Kreidberg JA. Applications of adhesion molecule gene knockout cell lines. Methods Cell Biol. 2002; 69:309-24.
  55. Zent R, Bush KT, Pohl ML, Quaranta V, Koshikawa N, Wang Z, Kreidberg JA, Sakurai H, Stuart RO, Nigám SK. Involvement of laminin binding integrins and laminin-5 in branching morphogenesis of the ureteric bud during kidney development. Dev Biol. 2001 Oct 15; 238(2):289-302.
  56. Wei Y, Eble JA, Wang Z, Kreidberg JA, Chapman HA. Urokinase receptors promote beta1 integrin function through interactions with integrin alpha3beta1. Mol Biol Cell. 2001 Oct; 12(10):2975-86.
  57. Lin Y, Liu A, Zhang S, Ruusunen T, Kreidberg JA, Peltoketo H, Drummond I, Vainio S. Induction of ureter branching as a response to Wnt-2b signaling during early kidney organogenesis. Dev Dyn. 2001 Sep; 222(1):26-39.
  58. Klinowska TC, Alexander CM, Georges-Labouesse E, Van der Neut R, Kreidberg JA, Jones CJ, Sonnenberg A, Streuli CH. Epithelial development and differentiation in the mammary gland is not dependent on alpha 3 or alpha 6 integrin subunits. Dev Biol. 2001 May 15; 233(2):449-67.
  59. Menko AS, Kreidberg JA, Ryan TT, Van Bockstaele E, Kukuruzinska MA. Loss of alpha3beta1 integrin function results in an altered differentiation program in the mouse submandibular gland. Dev Dyn. 2001 Apr; 220(4):337-49.
  60. Kreidberg JA. Functions of alpha3beta1 integrin. Curr Opin Cell Biol. 2000 Oct; 12(5):548-53.
  61. DiPersio CM, van der Neut R, Georges-Labouesse E, Kreidberg JA, Sonnenberg A, Hynes RO. alpha3beta1 and alpha6beta4 integrin receptors for laminin-5 are not essential for epidermal morphogenesis and homeostasis during skin development. J Cell Sci. 2000 Sep; 113 ( Pt 17):3051-62.
  62. DiPersio CM, Shao M, Di Costanzo L, Kreidberg JA, Hynes RO. Mouse keratinocytes immortalized with large T antigen acquire alpha3beta1 integrin-dependent secretion of MMP-9/gelatinase B. J Cell Sci. 2000 Aug; 113 ( Pt 16):2909-21.
  63. Kreidberg JA, Symons JM. Integrins in kidney development, function, and disease. Am J Physiol Renal Physiol. 2000 Aug; 279(2):F233-42.
  64. Dulabon L, Olson EC, Taglienti MG, Eisenhuth S, McGrath B, Walsh CA, Kreidberg JA, Anton ES. Reelin binds alpha3beta1 integrin and inhibits neuronal migration. Neuron. 2000 Jul; 27(1):33-44.
  65. Birk OS, Casiano DE, Wassif CA, Cogliati T, Zhao L, Zhao Y, Grinberg A, Huang S, Kreidberg JA, Parker KL, Porter FD, Westphal H. The LIM homeobox gene Lhx9 is essential for mouse gonad formation. Nature. 2000 Feb 24; 403(6772):909-13.
  66. Yang JT, Bader BL, Kreidberg JA, Ullman-Culleré M, Trevithick JE, Hynes RO. Overlapping and independent functions of fibronectin receptor integrins in early mesodermal development. Dev Biol. 1999 Nov 15; 215(2):264-77.
  67. Wang Z, Symons JM, Goldstein SL, McDonald A, Miner JH, Kreidberg JA. (Alpha)3(beta)1 integrin regulates epithelial cytoskeletal organization. J Cell Sci. 1999 Sep; 112 ( Pt 17):2925-35.
  68. De Arcangelis A, Mark M, Kreidberg J, Sorokin L, Georges-Labouesse E. Synergistic activities of alpha3 and alpha6 integrins are required during apical ectodermal ridge formation and organogenesis in the mouse. Development. 1999 Sep; 126(17):3957-68.
  69. Moore AW, McInnes L, Kreidberg J, Hastie ND, Schedl A. YAC complementation shows a requirement for Wt1 in the development of epicardium, adrenal gland and throughout nephrogenesis. Development. 1999 May; 126(9):1845-57.
  70. Kreidberg JA, Natoli TA, McGinnis L, Donovan M, Biggers JD, Amstutz A. Coordinate action of Wt1 and a modifier gene supports embryonic survival in the oviduct. Mol Reprod Dev. 1999 Apr; 52(4):366-75.
  71. Anderson R, Fässler R, Georges-Labouesse E, Hynes RO, Bader BL, Kreidberg JA, Schaible K, Heasman J, Wylie C. Mouse primordial germ cells lacking beta1 integrins enter the germline but fail to migrate normally to the gonads. Development. 1999 Apr; 126(8):1655-64.
  72. Anton ES, Kreidberg JA, Rakic P. Distinct functions of alpha3 and alpha(v) integrin receptors in neuronal migration and laminar organization of the cerebral cortex. Neuron. 1999 Feb; 22(2):277-89.
  73. Donovan MJ, Natoli TA, Sainio K, Amstutz A, Jaenisch R, Sariola H, Kreidberg JA. Initial differentiation of the metanephric mesenchyme is independent of WT1 and the ureteric bud. Dev Genet. 1999; 24(3-4):252-62.
  74. Ivins JK, Colognato H, Kreidberg JA, Yurchenco PD, Lander AD. Neuronal receptors mediating responses to antibodyactivated laminin-1. J Neurosci. 1998 Dec 1; 18(23):9703-15.
  75. Hodivala-Dilke KM, DiPersio CM, Kreidberg JA, Hynes RO. Novel roles for alpha3beta1 integrin as a regulator of cytoskeletal assembly and as a trans-dominant inhibitor of integrin receptor function in mouse keratinocytes. J Cell Biol. 1998 Sep 7; 142(5):1357-69.
  76. DiPersio CM, Hodivala-Dilke KM, Jaenisch R, Kreidberg JA, Hynes RO. alpha3beta1 Integrin is required for normal development of the epidermal basement membrane. J Cell Biol. 1997 May 5; 137(3):729-42.
  77. Englert C, Maheswaran S, Garvin AJ, Kreidberg J, Haber DA. Induction of p21 by the Wilms' tumor suppressor gene WT1. Cancer Res. 1997 Apr 15; 57(8):1429-34.
  78. Sainio K, Hellstedt P, Kreidberg JA, Saxén L, Sariola H. Differential regulation of two sets of mesonephric tubules by WT-1. Development. 1997 Apr; 124(7):1293-9.
  79. Kreidberg JA, Donovan MJ, Goldstein SL, Rennke H, Shepherd K, Jones RC, Jaenisch R. Alpha 3 beta 1 integrin has a crucial role in kidney and lung organogenesis. Development. 1996 Nov; 122(11):3537-47.
  80. Kreidberg JA. Gene targeting in kidney development. Med Pediatr Oncol. 1996 Nov; 27(5):445-52.
  81. Gardner H, Kreidberg J, Koteliansky V, Jaenisch R. Deletion of integrin alpha 1 by homologous recombination permits normal murine development but gives rise to a specific deficit in cell adhesion. Dev Biol. 1996 May 1; 175(2):301-13.
  82. Kreidberg JA, Sariola H, Loring JM, Maeda M, Pelletier J, Housman D, Jaenisch R. WT-1 is required for early kidney development. Cell. 1993 Aug 27; 74(4):679-91.
  83. Kreidberg JA, Kelly TJ. Genetic analysis of the human thymidine kinase gene promoter. Mol Cell Biol. 1986 Aug; 6(8):2903-9.
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