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Optical Microscopy Resource

The Optical Microscopy Resource (OMR) provides Boston Children’s Hospital and Longwood Medical Area researchers access to high-end confocal, spinning disc and intravital multiphoton microscopy. The facility provides assistance with all imaging procedures as needed by the investigators. The OMR provides Longwood Medical Area researchers the ability and expertise to obtain high quality imaging-based data from tissue and cell culture-based research studies.


Facility ILocated in the Warren Alpert Building at 200 Longwood Avenue and houses the Olympus FV1200MPE Multiphoton System, and the Marianas Spinning Disc System.

Facility II:  Located in the Center for Life Science at 3 Blackfan Circle and houses the Olympus FV1000 System




   

Olympus Fluoview FV1000 Confocal System


Configuration:  
Inverted, with Olympus IX 81 microscope

Laser Lines:  
405, 457, 488, 515, 559 and 635nm

Detectors:  
4 fluorescence + 1 transmission; DIC and spectral imaging capability


 

Marianas Spinning Disc System


Configuration: 
I
nverted, with Zeiss Axiovert 200M microscope   and full environmental enclosure chamber


Laser Lines:
 
491, 561, and 462nm


Detectors:  

EM-CCD for confocal; Wide-field CCD

 

 

Olympus FV1200MPE Multiphoton / Intravital System

Configuration:  
Upright, with Olympus BX61WI microscope

Laser Lines:  
Tunable, 609-1020nm

Detectors:  

4 non-descanned
 



                    

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