The Bioinformatics Core provides the computational infrastructure to facilitate efficient and seamless informational cross-talk between the different Cores of the Genomics Program. Additionally, we offer individualized support and collaboration in the design of integrative genomics studies and data analysis-interpretation with study projects using any Genomics Core. Our scope of work includes:
- Reviewing all relevant statistical and bioinformatical considerations prior to the implementation of multi-factorial genome-scale studies.
- Maintaining a secure, web-accessible and MIAME-compliant database for molecular data (e.g., genotype, RNA expression profile) storage, curation and basic data analysis.
- Implementing state-of-the-art data analyses and mathematical models of molecular data (genome, transcriptome, proteome, phenome) to optimally address the specific aims of respective study projects using the Genomics Cores.
- Taking an integrative genomics approach to data analysis. For example, when and where relevant, external bio-data from the public domain (e.g., NIH-NCBI's Gene Expression Omnibus and EMBL-EBI's ArrayExpress) will be included in our analyses, serving both as rational means of in silico validation (via independent test datasets) and for prioritizing candidate molecules/pathways for further functional validation in the laboratory.
- Scientific collaborations with study projects using the Genomics Cores.
Click here to go to the Bioinformatics Core website.
Flow Cytometry Core
The Cell Sorter Core offers state-of-the-art sorting applications in a manner that is cost effective, carefully controlled, and a nidus for collaborations. This flow cytometry and sorting facility has been available to investigators of the MRRC since 1978 and reflects the exceptional benefit to this MRRC of our relationships with the Howard Hughes Medical Institute (HHMI), which has shared in the purchase and maintenance of our Vantage SE high speed sorter. Investigators use the cell sorter for a wide range of applications, including flow karyotyping, chromosome sorting, cell cycle analysis, stem cell isolation, green fluorescent protein (GFP) containing cell sorting, and immunocytochemistry.
Molecular Genetics Core
The Molecular Genetics Core Facility provides high quality DNA sequence data from cloned DNA fragments in various vectors and from PCR-generated fragments. We also offer a microsatellite and single nucleotide polymorphism (SNP) marker genotyping service that includes genome-wide screening of families or sib-pairs for linkage to inherited disorders.
Click here to go to the Molecular Genetics Core website.