Current Laboratory Members:
Principle Investigator : Dr. Emanuela Gussoni
Dr. Gussoni obtained her Ph.D. degree from the University of Milan, Italy. She did her first postdoctoral fellowship at Stanford University, where she evaluated the survival of donor cells in DMD patients that participated in a myoblast transfer clinical trial. She then moved to Boston Children’s Hospital for her second postdoctoral fellowship training, where she studied muscle and bone marrow stem cells and their ability to deliver dystrophin following systemic injection in the laboratory of Dr. Louis Kunkel from 1994-1999. She established her own laboratory at Boston Children’s Hospital in early 2000. She has been a permanent member of the Muscular Dystrophy Association (USA) Scientific Advisory Committee since 2006. To view Dr. Gussoni's publications via PubMed Click Here.
Masters Student: Alessandro Colletta, MS
Alessandro received his Bachelor of Science degree from the University of Michigan in 2012 where he majored in Microbiology. After graduation, he joined an analytical chemistry lab and explored the potential applications of electrochemistry to the fields of microbiology and infectious diseases. In 2014, he came to Boston and started Masters in the Medical Sciences, an experience which further sparked his interest in medicine and strenghned his belief to continue this career path. In September 2015, he joined the Gussoni lab and successfully completed work for his Masters Research thesis. In this lab, he acquired new knowledge in skeletal muscle physiology and further appreciated how the study of molecular interactions can help better understand the mechanisms of disease processes. He will soon embark on a new journey and start medical school at Boston University in the fall of 2016.
Research Assistant/Laboratory Manager: Katlynn M Gwilt
Katlynn received her Bachelors of Science in Biology from Syracuse University, New York. After graduation, Katlynn discovered a passion for pharmacology while working full time in a Pharmacy. After taking courses in pharmacy and pharmaoclogy, she moved to Boston MA in 2014 where she began working at Boston Children’s Hospital. Katlynn joined Dr. Gussoni’s Lab in late 2015 where she currently serves as the Lab Manager and Research Assistant for the lab. To pursue her passion in pharmacology, Katlynn is enrolled in the evening Masters program in Pharmacology at Northeastern University. She will be conducting research towards her Master’s degree with Dr. Gussoni, and is expected to graduate in May of 2017. Katlynn's research interests include membrane protein interactions and receptor signal transduction pathways.
For more in-depth professional information on Katlynn, please visit her LinkedIn page or contact her at email@example.com
Undergraduate Student: Benton Mitchell
Dr. Regina Sohn joined the lab in March 2002. She is investigating the mechanisms of vertebrate myoblast fusion. Much is known about Drosophila muscle cells fusion, but less is known for mouse or human cells. Regina is studying the role of nephrin, the vertebrate orthologue of Drosophila SNS, in vertebrate myoblast fusion.
Clinical Genetics Fellow
Dr. Natasha Frank started working in our lab in August 2002 as a Clinical Genetics Fellow. She is studying genes specifically up-regulated/downregulated in muscle SP cells, thought to be a primitive cell type in muscle, versus MP cells, thought to be more committed myogenic progenitors. She has performed these studies on human fetal muscle cells derived from 10 individuals. The genes found to be up-regulated and down-regulated in muscle SP and MP cells according to prospective function are shown below. Natasha studied the function of up-regulated expression of BMP4 in muscle-derived SP cells, which triggers the proliferation of a population of myogenic cells expressing the BMP-receptor 1A. The BMP4-antagonist Gremlin, which is upregulated in MP cells, can block this proliferation.
Melissa Wu joined the Gussoni lab in July 2006, as the first graduate student. Her research interest is in adult stem cells. She is currently working on characterizing a protein that we believe is involved in differentiation or fusion of muscle stem cells. This protein, an orphan G-protein coupled receptor, is upregulated during early fusion events. Inhibition of its mRNA results in a block of differentiation and a resulting loss of fusion in mouse myoblasts. Her studies aim to identify this protein's role in muscle stem cell differentiation or fusion, including identifying its signaling pathways. Melissa graduated from MIT with an SB in Biology, where she worked with Dr. James Sherley at MIT and Dr. Ting Wu at Harvard on immortal DNA strand mechanisms in fly ovarian germline stem cells.
Matthew Mitchell is currently working on delivery of purified populations of muscle stem cells to mice to assess engraftment. Matt is working with the cellular marker Aldehyde Dehydrogenase (ALDH). ALDH was first identified as a marker for stem/progenitor cells located in the bone marrow. Continuing on work done by Mike Molloy, Matt has isolated ALDH positive cells from human fetal muscle tissue. These cells have been used in both in vitro and in vivo (using the murine model) projects to study their place in the myogenic lineage and engraftment capabilities, respectively.
John Silva is a Harvard undergraduate working on completing his thesis in our lab. He is studying various progenitor populations to determine which populations are likely to be most effective in cell-based therapies of muscular dystrophy. These populations include cells isolated from bone marrow, as well as from skeletal muscle.