Wai Man Jo Chan

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Wai-Man (Jo) Chan, B.S., Research Technologist

Wai-Man (Jo) Chan is a Senior Research Technologist and Lab Manager in the Engle lab. She received her B.S. in Biology from Northeastern University in 2001 and joined the Engle the same year. In the Engle lab, Ms. Chan is responsible for all mutation analysis; she undertakes radioactive linkage and mutational screening utilizing sequencing and as well as WAVE denaturing high performance liquid chromatography on disorder-causing genes which have been identified in our laboratory, conducts her own research to identify new disease genes, and teaches these techniques to students and fellows in the lab. She also writes computer programs to direct the STARlet liquid handling workstation to assemble PCR reactions for direct sequencing and WAVE analysis. She works closely with both the clinical neurogenetics and the basic neuroscience groups in the lab.

Publications:

  1. Mackey DA, Chan WM, Chan C, Gillies WE, Brooks AM, O'Day J, Engle EC. Congenital fibrosis of the vertically acting extraocular muscles maps to the FEOM3 locus. Hum Genet. 2002 May;110(5):510-512.

  2. Al-Baradie R, Yamada K, St Hilaire C, Chan WM, Andrews C, McIntosh N, Nakano M, Martonyi EJ, Raymond WR, Okumura S, Okihiro MM, Engle EC. Duane radial ray syndrome (Okihiro syndrome) maps to 20q13 and results from mutations in SALL4, a new member of the SAL family. Am J Hum Genet. 2002;71(5):1195-1199.

  3. Uyama E, Yamada K, Kawano H, Chan WM, Andrews C, Yoshioka M, Uchino M, Engle EC. A Japanese family with FEOM1-linked congenital fibrosis of the extraocular muscles type 1 associated with spinal canal stenosis and refinement of the FEOM1 critical region. Neuromuscul Disord. 2003;13(6):472-478.

  4. Yamada K, Andrews C, Chan WM, McKeown CA, Magli A, de Berardinis T, Loewenstein A, Lazar M, O'Keefe M, Letson R, London A, Ruttum M, Matsumoto N, Saito N, Morris L, Del Monte M, Johnson RH, Uyama E, Houtman WA, de Vries B, Carlow TJ, Hart BL, Krawiecki N, Shoffner J, Vogel MC, Katowitz J, Goldstein SM, Levin AV, Sener EC, Ozturk BT, Akarsu AN, Brodsky MC, Hanisch F, Cruse RP, Zubcov AA, Robb RM, Roggenkaemper P, Gottlob I, Kowal L, Battu R, Traboulsi EI, Franceschini P, Newlin A, Demer JL, Engle EC. Heterozygous mutations of the kinesin KIF21A in congenital fibrosis of the extraocular muscles type 1 (CFEOM1). Nat Genet. 2003;35(4):318-321.

  5. Yazdani A, Chung DC, Abbaszadegan MR, Al-Khayer K, Chan WM, Yazdani M, Ghodsi K, Engle EC, Traboulsi EI. A novel PHOX2A/ARIX mutation in an Iranian family with congenital fibrosis of extraocular muscles type 2 (CFEOM2). Am J Ophthalmol. 2003 Nov;136(5):861-865.

  6. Jen JC, Chan WM, Bosley TM, Wan J, Carr JR, Rub U, Shattuck D, Salamon G, Kudo LC, Ou J, Lin DD, Salih MA, Kansu T, Al Dhalaan H, Al Zayed Z, MacDonald DB, Stigsby B, Plaitakis A, Dretakis EK, Gottlob I, Pieh C, Traboulsi EI, Wang Q, Wang L, Andrews C, Yamada K, Demer JL, Karim SS, Alger JR, Geschwind DH, Deller T, Sicotte NL, Nelson SF, Baloh RW, Engle EC. Mutations in a human ROBO gene disrupt hindbrain axon pathway crossing and morphogenesis. Science. 2004 Apr;304:1509-1513.

  7. Yamada K, Chan WM, Andrews C, Bosley TM, Sener EC, Zwaan JT, Mullaney PB, Ozturk BT, Akarsu AN, Sabol LJ, Demer JL, Sullivan TJ, Gottlob I, Roggenkaemper P, Mackey DA, De Uzcategui CE, Uzcategui N, Ben-Zeev B, Traboulsi EI, Magli A, De Berardinis T, Gagliardi V, Awasthi-Patney S, Vogel MC, Rizzo JF 3rd, Engle EC. Identification of KIF21A mutations as a rare cause of Congenital Fibrosis of the Extraocular Muscles Type 3 (CFEOM3) Invest Ophthalmol Vis Sci. 2004 Jul;45(7):2218-2223.

  8. Tischfield MA, Bosley TM, Salih MA, Alorainy IA, Sener EC, Nester MJ, Oystreck DT, Chan WM, Andrews C, Erickson RP, Engle EC. Homozygous HOXA1 mutations disrupt human brainstem, inner ear, cardiovascular and cognitive development. Nat Genet. 2005 Oct; 37(10):1035-1037.

  9. Chan W-M, Traboulsi EI, Arthur B, Friedman N, Andrews C, Engle EC. Horizontal Gaze Palsy with Progressive Scoliosis can result from compound heterozygous mutations in ROBO3. Journal of Medical Genetics, 2006;43(3):e11.

  10. Tischfield MA, Chan W-M, Grunert J-F, Andrews C, Engle EC. HOXA1 mutations are not a common cause of Duane Anomaly. American Journal of Medical Genetics A. 2006 140(8):900-902.

  11. Chan W-M, Andrews C, Dragan L, Fredrick D, Armstrong L, Lyons C, Geraghty MT, Hunter DG, Yazdani A, Traboulsi EI, Pott JWR, Gutowski NJ, Ellard S, Young E, Hanisch F, Koc F, Schnall B, Engle EC. Three novel mutations in KIF21A highlight the importance of the third coiled-coil stalk domain in the etiology of CFEOM1. BMC Genetics, 2007;8(1):26.

  12. Dumars S, Andrews C, Chan W-M, Engle EC, Demer JL. Magnetic resonance imaging of the endophenotype of a novel familial Moebius-like syndrome. J AAPOS. 2008;12(4):381-9.

  13. Miyake N, Chilton J, Psatha M, Cheng L, Andrews C, Chan W-M, Law K, Crosier M, Lindsay S, Cheung M, Allen J, Gutowski NJ, Ellard S, Young E, Iannaccone A, Appukuttan B, Stout JT, Christiansen S, Ciccarelli ML, Baldi A, Campioni M, Zenteno JC, Davenport D, Mariani LE, Sahin M, Guthrie S, Engle EC. Human CHN1 mutations hyperactivate α2-chimaerin and cause Duane’s retraction syndrome. Science, 2008;321(5890):839-843.

  14. Oystreck DT, Khan AO, Vila-Coro AA, Oworu O, Al-Tassan N, Chan WM, Engle EC, Bosley TM. Synergistic divergence: a distinct ocular motility dysinnervation pattern. IOVS, 2009: 50(11):5213-5216 [Epub ahead of print Jul 2]

  15. Miyake N, Andrews C, Fan W, He W, Chan W-M, Engle EC. CNH1 mutations are not a common cause of sporadic Duane’s retraction syndrome. Am J Med Genet A, 2010;152A(1):215-217.

  16. Tischfield MA, Baris HN, Wu C, Rudolph G, Van Maldergem L, He W, Chan W-M, Andrews C, Demer JL, Robertson RL, Mackey DA, Ruddle JB, Bird TD, Gottlob I, Pieh C, Traboulsi EI, Pomeroy SL, Hunter DG, Soul JS, Newlin A, Sabol LJ, Doherty EJ, de Uzcategui CE, De Uzcategui N, Collins ML, Sener EC, Wabbels, B, Hellebrand H, Meitinger T, de Berandinis T, Magli A, Schiavi, C, Pastore-Trossello M, Koc F, Wong AM, Levin AV, Geraghty MT, Descartes M, Flaherty MP, Jamieson RV, Moller HU, Meuthen I, Callen DF, Kerwin J, Lindsay S, Meindl A, Gupta ML Jr, Pellman D, Engle EC. Human TUBB3 mutations perturb microtubule dynamics, kinesin interactions, and axon guidance. Cell. 2010; 140: 74-87.

  17. Rankin JK, Andrews C, Chan WM, Engle, EC. HOXA1 mutations are not a common cause of Mobius syndrome. J AAPOS. 2010 Feb;14(1):78-80.

    Contact information:

    Wai-Man (Jo) Chan, B.S.
    300 Longwood Avenue
    CLS 14030.1 Boston, MA 02115
    Fax: 617-919-2769
    E-mail: wchan@enders.tch.harvard.edu

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