Robert Husson, MD

Robert Husson, MD

Senior Physician in Medicine

Professor of Pediatrics, Harvard Medical School

  • Contact: 617-919-2900

  • Fax: 617-730-0254

Medical Services

Specialties

  • Tuberculosis and other mycobacterial infections
  • Bacterial Infections
  • Infectious Disease

Departments

  • Infectious Diseases
  • Medicine

Programs

  • Division of Infectious Diseases
To schedule an appointment: Call 617-919-2900

Experience and Education

Education

Medical School

Harvard Medical School,

Boston, MA

Fellowship

Pediatric Infectious Diseases - Boston Children's Hospital,

Boston, MA

Internship

Pediatrics - Boston Children's Hospital,

Boston, MA

Residency

Pediatrics - Boston Children's Hospital,

Boston, MA

Certifications

  • Pediatrics

  • Pediatric Infectious Diseases

Publications

Publications powered by Harvard Catalyst Profiles
  1. Sharp JD, Singh AK, Park ST, Lyubetskaya A, Peterson MW, Gomes AL, Potluri LP, Raman S, Galagan JE, Husson RN. Comprehensive Definition of the SigH Regulon of Mycobacterium tuberculosis Reveals Transcriptional Control of Diverse Stress Responses. PLoS One. 2016; 11(3):e0152145.
  2. Schifano JM, Cruz JW, Vvedenskaya IO, Edifor R, Ouyang M, Husson RN, Nickels BE, Woychik NA. tRNA is a new target for cleavage by a MazF toxin. Nucleic Acids Res. 2016 Feb 18; 44(3):1256-70.
  3. Cruz JW, Sharp JD, Hoffer ED, Maehigashi T, Vvedenskaya IO, Konkimalla A, Husson RN, Nickels BE, Dunham CM, Woychik NA. Growth-regulating Mycobacterium tuberculosis VapC-mt4 toxin is an isoacceptor-specific tRNase. Nat Commun. 2015; 6:7480.
  4. Prisic S, Hwang H, Dow A, Barnaby O, Pan TS, Lonzanida JA, Chazin WJ, Steen H, Husson RN. Zinc regulates a switch between primary and alternative S18 ribosomal proteins in Mycobacterium tuberculosis. Mol Microbiol. 2015 Jul; 97(2):263-80.
  5. Prisic S, Husson RN. Mycobacterium tuberculosis Serine/Threonine Protein Kinases. Microbiol Spectr. 2014 Oct; 2(5).
  6. Anandan T, Han J, Baun H, Nyayapathy S, Brown JT, Dial RL, Moltalvo JA, Kim MS, Yang SH, Ronning DR, Husson RN, Suh J, Kang CM. Phosphorylation regulates mycobacterial proteasome. J Microbiol. 2014 Sep; 52(9):743-54.
  7. Mir M, Prisic S, Kang CM, Lun S, Guo H, Murry JP, Rubin EJ, Husson RN. Mycobacterial gene cuvA is required for optimal nutrient utilization and virulence. Infect Immun. 2014 Oct; 82(10):4104-17.
  8. Schifano JM, Edifor R, Sharp JD, Ouyang M, Konkimalla A, Husson RN, Woychik NA. Mycobacterial toxin MazF-mt6 inhibits translation through cleavage of 23S rRNA at the ribosomal A site. Proc Natl Acad Sci U S A. 2013 May 21; 110(21):8501-6.
  9. Chou MF, Prisic S, Lubner JM, Church GM, Husson RN, Schwartz D. Using bacteria to determine protein kinase specificity and predict target substrates. PLoS One. 2012; 7(12):e52747.
  10. Sharp JD, Cruz JW, Raman S, Inouye M, Husson RN, Woychik NA. Growth and translation inhibition through sequence-specific RNA binding by Mycobacterium tuberculosis VapC toxin. J Biol Chem. 2012 Apr 13; 287(16):12835-47.
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  12. Ymele-Leki P, Cao S, Sharp J, Lambert KG, McAdam AJ, Husson RN, Tamayo G, Clardy J, Watnick PI. A high-throughput screen identifies a new natural product with broad-spectrum antibacterial activity. PLoS One. 2012; 7(2):e31307.
  13. Peters JM, Madhavan VL, Kazacos KR, Husson RN, Dangoudoubiyam S, Soul JS. Good outcome with early empiric treatment of neural larva migrans due to Baylisascaris procyonis. Pediatrics. 2012 Mar; 129(3):e806-11.
  14. Mir M, Asong J, Li X, Cardot J, Boons GJ, Husson RN. The extracytoplasmic domain of the Mycobacterium tuberculosis Ser/Thr kinase PknB binds specific muropeptides and is required for PknB localization. PLoS Pathog. 2011 Jul; 7(7):e1002182.
  15. Kang CM, Jahng WJ, Husson RN, Lee SH. A modified immunoblot method to identify substrates of protein kinases. J Microbiol. 2011 Jun; 49(3):499-501.
  16. Prisic S, Dankwa S, Schwartz D, Chou MF, Locasale JW, Kang CM, Bemis G, Church GM, Steen H, Husson RN. Extensive phosphorylation with overlapping specificity by Mycobacterium tuberculosis serine/threonine protein kinases. Proc Natl Acad Sci U S A. 2010 Apr 20; 107(16):7521-6.
  17. Pandey AK, Raman S, Proff R, Joshi S, Kang CM, Rubin EJ, Husson RN, Sassetti CM. Nitrile-inducible gene expression in mycobacteria. Tuberculosis (Edinb). 2009 Jan; 89(1):12-6.
  18. Park ST, Kang CM, Husson RN. Regulation of the SigH stress response regulon by an essential protein kinase in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2008 Sep 2; 105(35):13105-10.
  19. Zhu L, Phadtare S, Nariya H, Ouyang M, Husson RN, Inouye M. The mRNA interferases, MazF-mt3 and MazF-mt7 from Mycobacterium tuberculosis target unique pentad sequences in single-stranded RNA. Mol Microbiol. 2008 Aug; 69(3):559-69.
  20. Kang CM, Nyayapathy S, Lee JY, Suh JW, Husson RN. Wag31, a homologue of the cell division protein DivIVA, regulates growth, morphology and polar cell wall synthesis in mycobacteria. Microbiology. 2008 Mar; 154(Pt 3):725-35.
  21. Song T, Song SE, Raman S, Anaya M, Husson RN. Critical role of a single position in the -35 element for promoter recognition by Mycobacterium tuberculosis SigE and SigH. J Bacteriol. 2008 Mar; 190(6):2227-30.
  22. Williams DL, Pittman TL, Deshotel M, Oby-Robinson S, Smith I, Husson R. Molecular basis of the defective heat stress response in Mycobacterium leprae. J Bacteriol. 2007 Dec; 189(24):8818-27.
  23. Husson RN. Leaving on the lights: host-specific derepression of Mycobacterium tuberculosis gene expression by anti-sigma factor gene mutations. Mol Microbiol. 2006 Dec; 62(5):1217-9.
  24. Raman S, Puyang X, Cheng TY, Young DC, Moody DB, Husson RN. Mycobacterium tuberculosis SigM positively regulates Esx secreted protein and nonribosomal peptide synthetase genes and down regulates virulence-associated surface lipid synthesis. J Bacteriol. 2006 Dec; 188(24):8460-8.
  25. Hahn MY, Raman S, Anaya M, Husson RN. The Mycobacterium tuberculosis extracytoplasmic-function sigma factor SigL regulates polyketide synthases and secreted or membrane proteins and is required for virulence. J Bacteriol. 2005 Oct; 187(20):7062-71.
  26. Kang CM, Abbott DW, Park ST, Dascher CC, Cantley LC, Husson RN. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005 Jul 15; 19(14):1692-704.
  27. Kovacs A, Cowles MK, Britto P, Capparelli E, Fowler MG, Moye J, McIntosh K, Rathore MH, Pitt J, Husson RN. Pharmacokinetics of didanosine and drug resistance mutations in infants exposed to zidovudine during gestation or postnatally and treated with didanosine or zidovudine in the first three months of life. Pediatr Infect Dis J. 2005 Jun; 24(6):503-9.
  28. Raman S, Hazra R, Dascher CC, Husson RN. Transcription regulation by the Mycobacterium tuberculosis alternative sigma factor SigD and its role in virulence. J Bacteriol. 2004 Oct; 186(19):6605-16.
  29. Lerman SJ, Bernardo J, Daly JS, Husson R. Tuberculosis prevention in college students. J Am Coll Health. 2004 Sep-Oct; 53(2):53-8.
  30. Malley R, Stack AM, Husson RN, Thompson CM, Fleisher GR, Saladino RA. Development of a model of focal pneumococcal pneumonia in young rats. J Immune Based Ther Vaccines. 2004 Jan 23; 2(1):2.
  31. Song T, Dove SL, Lee KH, Husson RN. RshA, an anti-sigma factor that regulates the activity of the mycobacterial stress response sigma factor SigH. Mol Microbiol. 2003 Nov; 50(3):949-59.
  32. Raman S, Song T, Puyang X, Bardarov S, Jacobs WR, Husson RN. The alternative sigma factor SigH regulates major components of oxidative and heat stress responses in Mycobacterium tuberculosis. J Bacteriol. 2001 Oct; 183(20):6119-25.
  33. Hazra R, Floyd MM, Sloutsky A, Husson RN. Novel mycobacterium related to Mycobacterium triplex as a cause of cervical lymphadenitis. J Clin Microbiol. 2001 Apr; 39(4):1227-30.
  34. Hazra R, Lee SH, Maslow JN, Husson RN. Related strains of Mycobacterium avium cause disease in children with AIDS and in children with lymphadenitis. J Infect Dis. 2000 Apr; 181(4):1298-303.
  35. Robson CD, Hazra R, Barnes PD, Robertson RL, Jones D, Husson RN. Nontuberculous mycobacterial infection of the head and neck in immunocompetent children: CT and MR findings. AJNR Am J Neuroradiol. 1999 Nov-Dec; 20(10):1829-35.
  36. DeVincenzo JP, Berning SE, Peloquin CA, Husson RN. Multidrug-resistant tuberculosis meningitis: clinical problems and concentrations of second-line antituberculous medications. Ann Pharmacother. 1999 Nov; 33(11):1184-8.
  37. Fernandes ND, Wu QL, Kong D, Puyang X, Garg S, Husson RN. A mycobacterial extracytoplasmic sigma factor involved in survival following heat shock and oxidative stress. J Bacteriol. 1999 Jul; 181(14):4266-74.
  38. Rubin EJ, Akerley BJ, Novik VN, Lampe DJ, Husson RN, Mekalanos JJ. In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria. Proc Natl Acad Sci U S A. 1999 Feb 16; 96(4):1645-50.
  39. Hazra R, Robson CD, Perez-Atayde AR, Husson RN. Lymphadenitis due to nontuberculous mycobacteria in children: presentation and response to therapy. Clin Infect Dis. 1999 Jan; 28(1):123-9.
  40. Chong CY, Husson RN. Lack of acceptance of guidelines for prevention of disseminated Mycobacterium avium complex infection in infants and children infected with human immunodeficiency virus. Pediatr Infect Dis J. 1998 Dec; 17(12):1131-5.
  41. Husson RN. Homologous recombination in Mycobacterium smegmatis. Screening methods for detection of gene replacement. Methods Mol Biol. 1998; 101:199-206.
  42. Wu QL, Kong D, Lam K, Husson RN. A mycobacterial extracytoplasmic function sigma factor involved in survival following stress. J Bacteriol. 1997 May; 179(9):2922-9.
  43. Husson RN, Lan Y, Kojima E, Venzon D, Mitsuya H, McIntosh K. Vertical transmission of human immunodeficiency virus type 1: autologous neutralizing antibody, virus load, and virus phenotype. J Pediatr. 1995 Jun; 126(6):865-71.
  44. Mueller BU, Butler KM, Stocker VL, Balis FM, Brouwers P, Jarosinski P, Husson RN, Lewis LL, Venzon D, Pizzo PA. Clinical and pharmacokinetic evaluation of long-term therapy with didanosine in children with HIV infection. Pediatrics. 1994 Nov; 94(5):724-31.
  45. Mueller BU, Pizzo PA, Farley M, Husson RN, Goldsmith J, Kovacs A, Woods L, Ono J, Church JA, Brouwers P, et al. Pharmacokinetic evaluation of the combination of zidovudine and didanosine in children with human immunodeficiency virus infection. J Pediatr. 1994 Jul; 125(1):142-6.
  46. Husson RN, Ross LA, Sandelli S, Inderlied CB, Venzon D, Lewis LL, Woods L, Conville PS, Witebsky FG, Pizzo PA. Orally administered clarithromycin for the treatment of systemic Mycobacterium avium complex infection in children with acquired immunodeficiency syndrome. J Pediatr. 1994 May; 124(5 Pt 1):807-14.
  47. Husson RN, Mueller BU, Farley M, Woods L, Kovacs A, Goldsmith JC, Ono J, Lewis LL, Balis FM, Brouwers P, et al. Zidovudine and didanosine combination therapy in children with human immunodeficiency virus infection. Pediatrics. 1994 Feb; 93(2):316-22.
  48. Aldovini A, Husson RN, Young RA. The uraA locus and homologous recombination in Mycobacterium bovis BCG. J Bacteriol. 1993 Nov; 175(22):7282-9.
  49. Husson RN, Shirasaka T, Butler KM, Pizzo PA, Mitsuya H. High-level resistance to zidovudine but not to zalcitabine or didanosine in human immunodeficiency virus from children receiving antiretroviral therapy. J Pediatr. 1993 Jul; 123(1):9-16.
  50. Butler KM, Venzon D, Henry N, Husson RN, Mueller BU, Balis FM, Jacobsen F, Lewis LL, Pizzo PA. Pancreatitis in human immunodeficiency virus-infected children receiving dideoxyinosine. Pediatrics. 1993 Apr; 91(4):747-51.
  51. Robert-Guroff M, Roilides E, Muldoon R, Venzon D, Husson R, Marshall D, Gallo RC, Pizzo PA. Human immunodeficiency virus (HIV) type 1 strain MN neutralizing antibody in HIV-infected children: correlation with clinical status and prognostic value. J Infect Dis. 1993 Mar; 167(3):538-46.
  52. Shirasaka T, Yarchoan R, O'Brien MC, Husson RN, Anderson BD, Kojima E, Shimada T, Broder S, Mitsuya H. Changes in drug sensitivity of human immunodeficiency virus type 1 during therapy with azidothymidine, dideoxycytidine, and dideoxyinosine: an in vitro comparative study. Proc Natl Acad Sci U S A. 1993 Jan 15; 90(2):562-6.
  53. Husson RN, Saini R, Lewis LL, Butler KM, Patronas N, Pizzo PA. Cerebral artery aneurysms in children infected with human immunodeficiency virus. J Pediatr. 1992 Dec; 121(6):927-30.
  54. Mueller BU, Jacobsen F, Butler KM, Husson RN, Lewis LL, Pizzo PA. Combination treatment with azidothymidine and granulocyte colony-stimulating factor in children with human immunodeficiency virus infection. J Pediatr. 1992 Nov; 121(5 Pt 1):797-802.
  55. Lewis LL, Butler KM, Husson RN, Mueller BU, Fowler CL, Steinberg SM, Pizzo PA. Defining the population of human immunodeficiency virus-infected children at risk for Mycobacterium avium-intracellulare infection. J Pediatr. 1992 Nov; 121(5 Pt 1):677-83.
  56. Husson RN, Chung Y, Mordenti J, Butler KM, Chen S, Duliege AM, Brouwers P, Jarosinski P, Mueller BU, Ammann A, et al. Phase I study of continuous-infusion soluble CD4 as a single agent and in combination with oral dideoxyinosine therapy in children with symptomatic human immunodeficiency virus infection. J Pediatr. 1992 Oct; 121(4):627-33.
  57. Mueller BU, Butler KM, Higham MC, Husson RN, Montrella KA, Pizzo PA, Feuerstein IM, Manjunath K. Smooth muscle tumors in children with human immunodeficiency virus infection. Pediatrics. 1992 Sep; 90(3):460-3.
  58. Butler KM, Husson RN, Lewis LL, Mueller BU, Venzon D, Pizzo PA. CD4 status and P24 antigenemia. Are they useful predictors of survival in HIV-infected children receiving antiretroviral therapy? Am J Dis Child. 1992 Aug; 146(8):932-6.
  59. Roilides E, Butler KM, Husson RN, Mueller BU, Lewis LL, Pizzo PA. Pseudomonas infections in children with human immunodeficiency virus infection. Pediatr Infect Dis J. 1992 Jul; 11(7):547-53.
  60. Husson RN, Pizzo PA. The use of nucleoside analogues in the treatment of HIV-infected children. AIDS Res Hum Retroviruses. 1992 Jun; 8(6):1059-64.
  61. Butler KM, De Smet MD, Husson RN, Mueller B, Manjunath K, Montrella K, Lovato G, Jarosinski P, Nussenblatt RB, Pizzo PA. Treatment of aggressive cytomegalovirus retinitis with ganciclovir in combination with foscarnet in a child infected with human immunodeficiency virus. J Pediatr. 1992 Mar; 120(3):483-6.
  62. Whitcup SM, Butler KM, Caruso R, de Smet MD, Rubin B, Husson RN, Lopez JS, Belfort R, Pizzo PA, Nussenblatt RB. Retinal toxicity in human immunodeficiency virus-infected children treated with 2',3'-dideoxyinosine. Am J Ophthalmol. 1992 Jan 15; 113(1):1-7.
  63. Balis FM, Pizzo PA, Butler KM, Hawkins ME, Brouwers P, Husson RN, Jacobsen F, Blaney SM, Gress J, Jarosinski P, et al. Clinical pharmacology of 2',3'-dideoxyinosine in human immunodeficiency virus-infected children. J Infect Dis. 1992 Jan; 165(1):99-104.
  64. Mueller BU, Butler KM, Husson RN, Pizzo PA. Pneumocystis carinii pneumonia despite prophylaxis in children with human immunodeficiency virus infection. J Pediatr. 1991 Dec; 119(6):992-4.
  65. Roilides E, Marshall D, Venzon D, Butler K, Husson R, Pizzo PA. Bacterial infections in human immunodeficiency virus type 1-infected children: the impact of central venous catheters and antiretroviral agents. Pediatr Infect Dis J. 1991 Nov; 10(11):813-9.
  66. Butler KM, Husson RN, Balis FM, Brouwers P, Eddy J, el-Amin D, Gress J, Hawkins M, Jarosinski P, Moss H, et al. Dideoxyinosine in children with symptomatic human immunodeficiency virus infection. N Engl J Med. 1991 Jan 17; 324(3):137-44.
  67. Butler KM, Husson RN, Balis FM. Drug trial benefited HIV children. Nurs Times. 1991 Jan 30-Feb 5; 87(5):10.
  68. Pizzo PA, Butler K, Balis F, Brouwers E, Hawkins M, Eddy J, Einloth M, Falloon J, Husson R, Jarosinski P, et al. Dideoxycytidine alone and in an alternating schedule with zidovudine in children with symptomatic human immunodeficiency virus infection. J Pediatr. 1990 Nov; 117(5):799-808.
  69. Husson RN, Comeau AM, Hoff R. Diagnosis of human immunodeficiency virus infection in infants and children. Pediatrics. 1990 Jul; 86(1):1-10.
  70. Husson RN, James BE, Young RA. Gene replacement and expression of foreign DNA in mycobacteria. J Bacteriol. 1990 Feb; 172(2):519-24.
  71. Boom WH, Husson RN, Young RA, David JR, Piessens WF. In vivo and in vitro characterization of murine T-cell clones reactive to Mycobacterium tuberculosis. Infect Immun. 1987 Sep; 55(9):2223-9.
  72. Husson RN, Young RA. Genes for the major protein antigens of Mycobacterium tuberculosis: the etiologic agents of tuberculosis and leprosy share an immunodominant antigen. Proc Natl Acad Sci U S A. 1987 Mar; 84(6):1679-83.
  73. Oftung F, Mustafa AS, Husson R, Young RA, Godal T. Human T cell clones recognize two abundant Mycobacterium tuberculosis protein antigens expressed in Escherichia coli. J Immunol. 1987 Feb 1; 138(3):927-31.
  74. Orkin SH, Kolodner R, Michelson A, Husson R. Cloning and direct examination of a structurally abnormal human beta 0-thalassemia globin gene. Proc Natl Acad Sci U S A. 1980 Jun; 77(6):3558-62.
To schedule an appointment: Call 617-919-2900

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