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Judith Steen
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Judith A. J.  Steen, PhD

Department: Neurology
Research Centers: F.M. Kirby Neurobiology Center
Hospital Title: Associate in Neurology
Academic Title: Assistant Professor, Department of Neurobiology
Research Area: Neurodegenerative diseases
Proteomics and bioinformatics

Research Overview

  • The development of novel and improved mass spectrometric and proteomic methods for the analysis of complex protein mixtures and the detailed characterization of proteins within these mixtures, including the analysis of post-translational protein modifications and the quantitation of protein abundances.
  • Development of instrument platforms for the detection and identification of disease-markers with special emphasis on pediatric diseases.
  • The use of mass spectrometry to identify mechanisms of cell cycle control during neurogenesis.
  • Development and applications of strategies to solve systems biological questions and problems at the proteome level. To achieve our goals we use proteomic and mass spectrometric technology in addition to biochemistry and molecular biology approaches, cell culture systems and animal models, including the mouse, the rat, and the African clawfrog.

About Judith Steen

Judith Steen studied chemistry and biochemistry at the University of Toronto. She then studied mass spectrometry and cell biology under the mentorship of Peter Roepstorff (University of Southern Denmark) and Marc Kirschner (Harvard University), respectively.

Key Publications

  • Greer PL, Hanayama R, Bloodgood BL, Flavell SW, Mardinly AR, Lipton DM, Kim T, Griffith EC, Waldon Z, Maehr R, Ploegh HL, Chowdhury S, Worley PF, Steen JJ, Greenberg ME. The Angelman Syndrome-associated ubiquitin ligase Ube3A regulates synapse development by ubiquitinating Arc. Cell. 2010 Mar 5; 140(5):704-16.
     
  • Ozlu N, Monigatti F, Renard BY, Field CM, Steen H, Mitchison TJ, Steen JJ. Binding partner switching on microtubules and aurora-B in the mitosis to cytokinesis transition. 2009 Sep 28; Mol Cell Proteomics. 2010 Feb; 9(2):336-50.
     
  • Kirchner M, Renard BY, Kothe U, Hamprecht FA, Steen H, Steen JAJ. Computational protein coregulation screening for quantitative mass spectrometry experiments. Bioinformatics. 2010 Jan 1; 26(1):77-83.
     
  • Singh S, Springer M, Steen JA*, Kirschner MW, Steen H. FLEXIQuant A novel tool for the absolute quantification of proteins, and the simultaneous identification and quantification of potentially modified peptides. J Proteome Res. 2009; 8(5):2201-2210. *corresponding author
     
  • Steen, J. A., Steen, H., Georgi, A., Parker, K., Springer, M., Kirchner, M., Hamprecht, F. and Kirschner, M. W. Different phosphorylation states of the anaphase promoting complex in response to antimitotic drugs: a quantitative proteomic analysis. Proc Natl Acad Sci U S A. 2008; 105: 6069-74.
     
  • Steen, H., Jebanathirajah, J. A., Springer, M. and Kirschner, M. W. Stable isotope-free relative and absolute quantitation of protein phosphorylation stoichiometry by MS. Proc Natl Acad Sci U S A 2005; 102: 3948-53.

For a list of Judith Steen's publications on PubMed, click here.

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