Carlo Brugnara

Carlo Brugnara, MD

Director, Hematology Lab

Professor of Pathology

    Contact: 617-355-6347
  • Fax: 617-730-0383

Medical Services

Specialties

  • Clinical Pathology

Departments

  • Laboratory Medicine Research Center
To schedule an appointment: Call 617-355-6347

Experience and Education

Education

Medical School

University of Padua (Verona) Italy, 1979

Residency

Brigham and Women's Hospital, 07/86 to 06/89

Boston, MA

Fellowship

Harvard Medical School, 08/82 to 06/85

Boston, MA

Certifications

  • Clinical Pathology/Blood Banking/Transfusion Medicine

Publications

Publications powered by Harvard Catalyst Profiles
  1. Hennek JW, Kumar AA, Wiltschko AB, Patton MR, Lee SY, Brugnara C, Adams RP, Whitesides GM. Diagnosis of iron deficiency anemia using density-based fractionation of red blood cells. Lab Chip. 2016 Oct 5; 16(20):3929-3939.
  2. Andolfo I, Russo R, Manna F, De Rosa G, Gambale A, Zouwail S, Detta N, Pardo CL, Alper SL, Brugnara C, Sharma AK, De Franceschi L, Iolascon A. Functional characterization of novel ABCB6 mutations and their clinical implications in familial pseudohyperkalemia. Haematologica. 2016 Aug; 101(8):909-17.
  3. Brugnara C. The American Journal of Hematology turns 40. Am J Hematol. 2016 Jan; 91(1):4.
  4. Archer N, Galacteros F, Brugnara C. 2015 Clinical trials update in sickle cell anemia. Am J Hematol. 2015 Oct; 90(10):934-50.
  5. Andolfo I, Russo R, Manna F, Shmukler BE, Gambale A, Vitiello G, De Rosa G, Brugnara C, Alper SL, Snyder LM, Iolascon A. Novel Gardos channel mutations linked to dehydrated hereditary stomatocytosis (xerocytosis). Am J Hematol. 2015 Oct; 90(10):921-6.
  6. Archer NM, Brugnara C. Diagnosis of iron-deficient states. Crit Rev Clin Lab Sci. 2015; 52(5):256-72.
  7. Archer NM, Samnaliev M, Grace R, Brugnara C. The utility of the DDAVP challenge test in children with low von Willebrand factor. Br J Haematol. 2015 Sep; 170(6):884-6.
  8. Brugnara C. American Journal of Hematology, getting ready for the 40th birthday in 2016. Am J Hematol. 2015 Jan; 90(1):1.
  9. Sharma S, Brugnara C, Betensky RA, Waikar SS. Reductions in red blood cell 2,3-diphosphoglycerate concentration during continuous renal replacment therapy. Clin J Am Soc Nephrol. 2015 Jan 7; 10(1):74-9.
  10. Kumar AA, Chunda-Liyoka C, Hennek JW, Mantina H, Lee SY, Patton MR, Sambo P, Sinyangwe S, Kankasa C, Chintu C, Brugnara C, Stossel TP, Whitesides GM. Evaluation of a density-based rapid diagnostic test for sickle cell disease in a clinical setting in Zambia. PLoS One. 2014; 9(12):e114540.
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  12. Kratz A, Brugnara C. Automated hematology analyzers: state of the art. Clin Lab Med. 2015 Mar; 35(1):xiii-xiv.
  13. Piva E, Brugnara C, Spolaore F, Plebani M. Clinical utility of reticulocyte parameters. Clin Lab Med. 2015 Mar; 35(1):133-63.
  14. Bhaduri B, Kandel M, Brugnara C, Tangella K, Popescu G. Optical assay of erythrocyte function in banked blood. Sci Rep. 2014; 4:6211.
  15. Kumar AA, Patton MR, Hennek JW, Lee SY, D'Alesio-Spina G, Yang X, Kanter J, Shevkoplyas SS, Brugnara C, Whitesides GM. Density-based separation in multiphase systems provides a simple method to identify sickle cell disease. Proc Natl Acad Sci U S A. 2014 Oct 14; 111(41):14864-9.
  16. Rimmelé P, Bigarella CL, Liang R, Izac B, Dieguez-Gonzalez R, Barbet G, Donovan M, Brugnara C, Blander JM, Sinclair DA, Ghaffari S. Aging-like phenotype and defective lineage specification in SIRT1-deleted hematopoietic stem and progenitor cells. Stem Cell Reports. 2014 Jul 8; 3(1):44-59.
  17. Shmukler BE, Vandorpe DH, Rivera A, Auerbach M, Brugnara C, Alper SL. Dehydrated stomatocytic anemia due to the heterozygous mutation R2456H in the mechanosensitive cation channel PIEZO1: a case report. Blood Cells Mol Dis. 2014 Jan; 52(1):53-4.
  18. Brugnara C, Mohandas N. Red cell indices in classification and treatment of anemias: from M.M. Wintrobes's original 1934 classification to the third millennium. Curr Opin Hematol. 2013 May; 20(3):222-30.
  19. Rivera A, Zee RY, Alper SL, Peters LL, Brugnara C. Strain-specific variations in cation content and transport in mouse erythrocytes. Physiol Genomics. 2013 May 1; 45(9):343-50.
  20. Lim C, Hansen E, DeSimone TM, Moreno Y, Junker K, Bei A, Brugnara C, Buckee CO, Duraisingh MT. Expansion of host cellular niche can drive adaptation of a zoonotic malaria parasite to humans. Nat Commun. 2013; 4:1638.
  21. Brugnara C, Adamson J, Auerbach M, Kane R, Macdougall I, Mast A. Iron deficiency: what are the future trends in diagnostics and therapeutics? Clin Chem. 2013 May; 59(5):740-5.
  22. Shah DI, Takahashi-Makise N, Cooney JD, Li L, Schultz IJ, Pierce EL, Narla A, Seguin A, Hattangadi SM, Medlock AE, Langer NB, Dailey TA, Hurst SN, Faccenda D, Wiwczar JM, Heggers SK, Vogin G, Chen W, Chen C, Campagna DR, Brugnara C, Zhou Y, Ebert BL, Danial NN, Fleming MD, Ward DM, Campanella M, Dailey HA, Kaplan J, Paw BH. Mitochondrial Atpif1 regulates haem synthesis in developing erythroblasts. Nature. 2012 Nov 22; 491(7425):608-12.
  23. Bartolucci P, Brugnara C, Teixeira-Pinto A, Pissard S, Moradkhani K, Jouault H, Galacteros F. Erythrocyte density in sickle cell syndromes is associated with specific clinical manifestations and hemolysis. Blood. 2012 Oct 11; 120(15):3136-41.
  24. Kheir JN, Scharp LA, Borden MA, Swanson EJ, Loxley A, Reese JH, Black KJ, Velazquez LA, Thomson LM, Walsh BK, Mullen KE, Graham DA, Lawlor MW, Brugnara C, Bell DC, McGowan FX. Oxygen gas-filled microparticles provide intravenous oxygen delivery. Sci Transl Med. 2012 Jun 27; 4(140):140ra88.
  25. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A, Delanghe J. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Am J Hematol. 2012 Apr; 87(4):347-8.
  26. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Transfusion. 2012 Jun; 52(6):e17-9.
  27. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A, Delanghe J. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Clin Chem Lab Med. 2012 Mar; 50(3):411-3.
  28. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A, Delanghe J. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Scand J Clin Lab Invest. 2012 Apr; 72(2):89-91.
  29. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Clin Biochem. 2012 Feb; 45(3):185-6.
  30. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A, Delanghe J. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Statement by the Consortium of Laboratory Medicine Journal Editors. Ann Clin Biochem. 2012 Mar; 49(Pt 2):105-7.
  31. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A, Delanghe J. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Clin Chem. 2012 Mar; 58(3):483-5.
  32. Rifai N, Annesley TM, Berg JP, Brugnara C, Delvin E, Lamb EJ, Ness PM, Plebani M, Wick MR, Wu A, Delanghe J. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports. Clin Chim Acta. 2012 Apr 11; 413(7-8):653-5.
  33. Stewart AK, Shmukler BE, Vandorpe DH, Rivera A, Heneghan JF, Li X, Hsu A, Karpatkin M, O'Neill AF, Bauer DE, Heeney MM, John K, Kuypers FA, Gallagher PG, Lux SE, Brugnara C, Westhoff CM, Alper SL. Loss-of-function and gain-of-function phenotypes of stomatocytosis mutant RhAG F65S. Am J Physiol Cell Physiol. 2011 Dec; 301(6):C1325-43.
  34. Rifai N, Plebani M, Wu A, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full-disclosure in industry-sponsored laboratory medicine research studies: Statement by the Consortium of Laboratory Medicine Journal Editors. Am J Hematol. 2011 Mar; 86(3):244.
  35. Wang W, Brugnara C, Snyder C, Wynn L, Rogers Z, Kalinyak K, Brown C, Qureshi A, Bigelow C, Neumayr L, Smith-Whitley K, Chui DH, Delahunty M, Woolson R, Steinberg M, Telen M, Kesler K. The effects of hydroxycarbamide and magnesium on haemoglobin SC disease: results of the multi-centre CHAMPS trial. Br J Haematol. 2011 Mar; 152(6):771-6.
  36. Rifai N, Plebani M, Wu A, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full disclosure in industry-sponsored laboratory medicine research studies: statement by the Consortium of Laboratory Medicine Journal Editors. Ann Clin Biochem. 2011 Jan; 48(Pt 1):5-6.
  37. Stewart AK, Kedar PS, Shmukler BE, Vandorpe DH, Hsu A, Glader B, Rivera A, Brugnara C, Alper SL. Functional characterization and modified rescue of novel AE1 mutation R730C associated with overhydrated cation leak stomatocytosis. Am J Physiol Cell Physiol. 2011 May; 300(5):C1034-46.
  38. Rifai N, Plebani M, Wu A, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full disclosure in industry-sponsored laboratory medicine research studies: statement by the Consortium of Laboratory Medicine Journal Editors. Transfusion. 2012 Jun; 52(6):e15-6.
  39. Rifai N, Plebani M, Wu AH, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full disclosure in industry-sponsored laboratory medicine research studies: Statement by the Consortium of Laboratory Medicine Journal Editors. Clin Biochem. 2011 Feb; 44(2-3):149-50.
  40. Rifai N, Plebani M, Wu A, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full-disclosure in industry-sponsored laboratory medicine research studies: statement by the Consortium of Laboratory Medicine Journal Editors. Scand J Clin Lab Invest. 2011 May; 71(3):177-8.
  41. Rifai N, Plebani M, Wu A, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full disclosure in industry-sponsored laboratory medicine research studies: statement by the Consortium of Laboratory Medicine Journal Editors. Clin Chem. 2011 Mar; 57(3):359-60.
  42. Rifai N, Plebani M, Wu A, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full-disclosure in industry-sponsored laboratory medicine research studies: statement by the Consortium of Laboratory Medicine Journal Editors. Clin Chem Lab Med. 2011 Jan; 49(1):3-4.
  43. Rifai N, Plebani M, Wu AH, Delanghe J, Brugnara C, Delvin E, Lamb EJ, Ness PM, Wick MR, Berg JP. Full-disclosure in industry-sponsored laboratory medicine research studies: statement by the Consortium of Laboratory Medicine Journal Editors. Clin Chim Acta. 2011 Mar 18; 412(7-8):491-2.
  44. Bei AK, Brugnara C, Duraisingh MT. In vitro genetic analysis of an erythrocyte determinant of malaria infection. J Infect Dis. 2010 Dec 1; 202(11):1722-7.
  45. Peters LL, Shavit JA, Lambert AJ, Tsaih SW, Li Q, Su Z, Leduc MS, Paigen B, Churchill GA, Ginsburg D, Brugnara C. Sequence variation at multiple loci influences red cell hemoglobin concentration. Blood. 2010 Dec 16; 116(25):e139-49.
  46. Piva E, Brugnara C, Chiandetti L, Plebani M. Automated reticulocyte counting: state of the art and clinical applications in the evaluation of erythropoiesis. Clin Chem Lab Med. 2010 Oct; 48(10):1369-80.
  47. Vandorpe DH, Xu C, Shmukler BE, Otterbein LE, Trudel M, Sachs F, Gottlieb PA, Brugnara C, Alper SL. Hypoxia activates a Ca2+-permeable cation conductance sensitive to carbon monoxide and to GsMTx-4 in human and mouse sickle erythrocytes. PLoS One. 2010 Jan 15; 5(1):e8732.
  48. Ballas SK, McCarthy WF, Guo N, Brugnara C, Kling G, Bauserman RL, Waclawiw MA. Early detection of response to hydroxyurea therapy in patients with sickle cell anemia. Hemoglobin. 2010; 34(5):424-9.
  49. Brugnara C. An immunoassay for human serum hepcidin at last: Ganz klar? Blood. 2008 Nov 15; 112(10):3922-3.
  50. Licameli GR, Jones DT, Santosuosso J, Lapp C, Brugnara C, Kenna MA. Use of a preoperative bleeding questionnaire in pediatric patients who undergo adenotonsillectomy. Otolaryngol Head Neck Surg. 2008 Oct; 139(4):546-550.
  51. Shaker M, Jenkins P, Ullrich C, Brugnara C, Nghiem BT, Bernstein H. An economic analysis of anemia prevention during infancy. J Pediatr. 2009 Jan; 154(1):44-9.
  52. Katodritou E, Zervas K, Terpos E, Brugnara C. Use of erythropoiesis stimulating agents and intravenous iron for cancer and treatment-related anaemia: the need for predictors and indicators of effectiveness has not abated. Br J Haematol. 2008 Jul; 142(1):3-10.
  53. Hankins JS, Wynn LW, Brugnara C, Hillery CA, Li CS, Wang WC. Phase I study of magnesium pidolate in combination with hydroxycarbamide for children with sickle cell anaemia. Br J Haematol. 2008 Jan; 140(1):80-5.
  54. Bertin A, Mahaney MC, Cox LA, Rogers J, VandeBerg JL, Brugnara C, Platt OS. Quantitative trait loci for peripheral blood cell counts: a study in baboons. Mamm Genome. 2007 May; 18(5):361-72.
  55. Rust MB, Alper SL, Rudhard Y, Shmukler BE, Vicente R, Brugnara C, Trudel M, Jentsch TJ, Hübner CA. Disruption of erythroid K-Cl cotransporters alters erythrocyte volume and partially rescues erythrocyte dehydration in SAD mice. J Clin Invest. 2007 Jun; 117(6):1708-17.
  56. Brugnara C, de Franceschi L. [Clinical trials of new therapeutic pharmacology for sickle cell disease]. Sante. 2006 Oct-Dec; 16(4):263-8.
  57. Brugnara C, Schiller B, Moran J. Reticulocyte hemoglobin equivalent (Ret He) and assessment of iron-deficient states. Clin Lab Haematol. 2006 Oct; 28(5):303-8.
  58. Pysher TJ, Bach PR, Geaghan SM, Hamilton MS, Laposata M, Lockitch G, Brugnara C, Coffin CM, Pasquali M, Rinaldo P, Roberts WL, Rutledge JC, Ashwood ER, Blaylock RC, Campos JM, Goldsmith B, Jones PM, Lim M, Meikle AW, Perkins SL, Perry DA, Petti CA, Rogers BB, Steele PE, Weiss RL, Woods G. Teaching pediatric laboratory medicine to pathology residents. Arch Pathol Lab Med. 2006 Jul; 130(7):1031-8.
  59. Rivera A, De Franceschi L, Peters LL, Gascard P, Mohandas N, Brugnara C. Effect of complete protein 4.1R deficiency on ion transport properties of murine erythrocytes. Am J Physiol Cell Physiol. 2006 Nov; 291(5):C880-6.
  60. Peters LL, Lambert AJ, Zhang W, Churchill GA, Brugnara C, Platt OS. Quantitative trait loci for baseline erythroid traits. Mamm Genome. 2006 Apr; 17(4):298-309.
  61. Liu Y, Pop R, Sadegh C, Brugnara C, Haase VH, Socolovsky M. Suppression of Fas-FasL coexpression by erythropoietin mediates erythroblast expansion during the erythropoietic stress response in vivo. Blood. 2006 Jul 1; 108(1):123-33.
  62. De Franceschi L, Villa-Moruzzi E, Biondani A, Siciliano A, Brugnara C, Alper SL, Lowell CA, Berton G. Regulation of K-Cl cotransport by protein phosphatase 1alpha in mouse erythrocytes. Pflugers Arch. 2006 Mar; 451(6):760-8.
  63. Peters LL, Zhang W, Lambert AJ, Brugnara C, Churchill GA, Platt OS. Quantitative trait loci for baseline white blood cell count, platelet count, and mean platelet volume. Mamm Genome. 2005 Oct; 16(10):749-63.
  64. Ullrich C, Wu A, Armsby C, Rieber S, Wingerter S, Brugnara C, Shapiro D, Bernstein H. Screening healthy infants for iron deficiency using reticulocyte hemoglobin content. JAMA. 2005 Aug 24; 294(8):924-30.
  65. Stoffman N, Brugnara C, Woods ER. An algorithm using reticulocyte hemoglobin content (CHr) measurement in screening adolescents for iron deficiency. J Adolesc Health. 2005 Jun; 36(6):529.
  66. Mahaney MC, Brugnara C, Lease LR, Platt OS. Genetic influences on peripheral blood cell counts: a study in baboons. Blood. 2005 Aug 15; 106(4):1210-4.
  67. De Franceschi L, Rivera A, Fleming MD, Honczarenko M, Peters LL, Gascard P, Mohandas N, Brugnara C. Evidence for a protective role of the Gardos channel against hemolysis in murine spherocytosis. Blood. 2005 Aug 15; 106(4):1454-9.
  68. Mallozzi C, De Franceschi L, Brugnara C, Di Stasi AM. Protein phosphatase 1alpha is tyrosine-phosphorylated and inactivated by peroxynitrite in erythrocytes through the src family kinase fgr. Free Radic Biol Med. 2005 Jun 15; 38(12):1625-36.
  69. de Franceschi L, Turrini F, Honczarenko M, Ayi K, Rivera A, Fleming MD, Law T, Mannu F, Kuypers FA, Bast A, van der Vijgh WJ, Brugnara C. In vivo reduction of erythrocyte oxidant stress in a murine model of beta-thalassemia. Haematologica. 2004 Nov; 89(11):1287-98.
  70. Begenisich T, Nakamoto T, Ovitt CE, Nehrke K, Brugnara C, Alper SL, Melvin JE. Physiological roles of the intermediate conductance, Ca2+-activated potassium channel Kcnn4. J Biol Chem. 2004 Nov 12; 279(46):47681-7.
  71. Geltman PL, Meyers AF, Mehta SD, Brugnara C, Villon I, Wu YA, Bauchner H. Daily multivitamins with iron to prevent anemia in high-risk infants: a randomized clinical trial. Pediatrics. 2004 Jul; 114(1):86-93.
  72. Aune MW, Becker JL, Brugnara C, Canfield W, Dorfman DM, Fiehn W, Fischer G, Fitzpatrick P, Flaming TH, Henriksen HK, Kunicka JE, Lackner KJ, Minchello E, Mullenix PA, Myers M, Petersen A, Ternstrom W, Wilson SJ. Automated flow cytometric analysis of blood cells in cerebrospinal fluid: analytic performance. Am J Clin Pathol. 2004 May; 121(5):690-700.
  73. Warady BA, Kausz A, Lerner G, Brewer ED, Chadha V, Brugnara C, Dahl NV, Watkins SL. Iron therapy in the pediatric hemodialysis population. Pediatr Nephrol. 2004 Jun; 19(6):655-61.
  74. Brugnara C. Sickle cell disease: from membrane pathophysiology to novel therapies for prevention of erythrocyte dehydration. J Pediatr Hematol Oncol. 2003 Dec; 25(12):927-33.
  75. Brugnara C. Iron deficiency and erythropoiesis: new diagnostic approaches. Clin Chem. 2003 Oct; 49(10):1573-8.
  76. Weiner DL, Brugnara C. Hydroxyurea and sickle cell disease: a chance for every patient. JAMA. 2003 Apr 2; 289(13):1692-4.
  77. Weiner DL, Hibberd PL, Betit P, Cooper AB, Botelho CA, Brugnara C. Preliminary assessment of inhaled nitric oxide for acute vaso-occlusive crisis in pediatric patients with sickle cell disease. JAMA. 2003 Mar 5; 289(9):1136-42.
  78. Bize I, Taher S, Brugnara C. Regulation of K-Cl cotransport during reticulocyte maturation and erythrocyte aging in normal and sickle erythrocytes. Am J Physiol Cell Physiol. 2003 Jul; 285(1):C31-8.
  79. Brugnara C. A hematologic "gold standard" for iron-deficient states? Clin Chem. 2002 Jul; 48(7):981-2.
  80. Khanna R, Chang SH, Andrabi S, Azam M, Kim A, Rivera A, Brugnara C, Low PS, Liu SC, Chishti AH. Headpiece domain of dematin is required for the stability of the erythrocyte membrane. Proc Natl Acad Sci U S A. 2002 May 14; 99(10):6637-42.
  81. Coffin CM, Hamilton MS, Pysher TJ, Bach P, Ashwood E, Schweiger J, Monahan D, Perry D, Rogers BB, Brugnara C, Rutledge J, Weiss R, Ash O, Hill H, Meikle W, Roberts W, Geaghan S. Pediatric laboratory medicine: current challenges and future opportunities. Am J Clin Pathol. 2002 May; 117(5):683-90.
  82. Steinberg MH, Brugnara C. Developing treatment for sickle cell disease. Expert Opin Investig Drugs. 2002 May; 11(5):645-59.
  83. Holm TM, Braun A, Trigatti BL, Brugnara C, Sakamoto M, Krieger M, Andrews NC. Failure of red blood cell maturation in mice with defects in the high-density lipoprotein receptor SR-BI. Blood. 2002 Mar 1; 99(5):1817-24.
  84. Steinberg MH, Brugnara C. Pathophysiological-based approaches to treatment of sickle cell disease. Annu Rev Med. 2003; 54:89-112.
  85. Socolovsky M, Nam H, Fleming MD, Haase VH, Brugnara C, Lodish HF. Ineffective erythropoiesis in Stat5a(-/-)5b(-/-) mice due to decreased survival of early erythroblasts. Blood. 2001 Dec 1; 98(12):3261-73.
  86. Brugnara C, De Franceschi L, Beuzard Y. Erythrocyte-active agents and treatment of sickle cell disease. Semin Hematol. 2001 Oct; 38(4):324-32.
  87. Casula S, Shmukler BE, Wilhelm S, Stuart-Tilley AK, Su W, Chernova MN, Brugnara C, Alper SL. A dominant negative mutant of the KCC1 K-Cl cotransporter: both N- and C-terminal cytoplasmic domains are required for K-Cl cotransport activity. J Biol Chem. 2001 Nov 9; 276(45):41870-8.
  88. Brugnara C, De Franceschi L, Bennekou P, Alper SL, Christophersen P. Novel therapies for prevention of erythrocyte dehydration in sickle cell anemia. Drug News Perspect. 2001 May; 14(4):208-20.
  89. Bennekou P, de Franceschi L, Pedersen O, Lian L, Asakura T, Evans G, Brugnara C, Christophersen P. Treatment with NS3623, a novel Cl-conductance blocker, ameliorates erythrocyte dehydration in transgenic SAD mice: a possible new therapeutic approach for sickle cell disease. Blood. 2001 Mar 1; 97(5):1451-7.
  90. Brugnara C. Therapeutic strategies for prevention of sickle cell dehydration. Blood Cells Mol Dis. 2001 Jan-Feb; 27(1):71-80.
  91. Mueller BU, Brugnara C. Prevention of red cell dehydration: a possible new treatment for sickle cell disease. Pediatr Pathol Mol Med. 2001 Jan-Feb; 20(1):15-25.
  92. Parisotto R, Gore CJ, Hahn AG, Ashenden MJ, Olds TS, Martin DT, Pyne DB, Gawthorn K, Brugnara C. Reticulocyte parameters as potential discriminators of recombinant human erythropoietin abuse in elite athletes. Int J Sports Med. 2000 Oct; 21(7):471-9.
  93. Bize I, Güvenç B, Buchbinder G, Brugnara C. Stimulation of human erythrocyte K-Cl cotransport and protein phosphatase type 2A by n-ethylmaleimide: role of intracellular Mg++. J Membr Biol. 2000 Sep 15; 177(2):159-68.
  94. Goodnough LT, Skikne B, Brugnara C. Erythropoietin, iron, and erythropoiesis. Blood. 2000 Aug 1; 96(3):823-33.
  95. Humbert PO, Rogers C, Ganiatsas S, Landsberg RL, Trimarchi JM, Dandapani S, Brugnara C, Erdman S, Schrenzel M, Bronson RT, Lees JA. E2F4 is essential for normal erythrocyte maturation and neonatal viability. Mol Cell. 2000 Aug; 6(2):281-91.
  96. Hansen AR, Lapp C, Brugnara C. Plasminogen activator inhibitor-1: defining characteristics in the cerebrospinal fluid of newborns. J Pediatr. 2000 Jul; 137(1):132-4.
  97. Parisotto R, Gore CJ, Emslie KR, Ashenden MJ, Brugnara C, Howe C, Martin DT, Trout GJ, Hahn AG. A novel method utilising markers of altered erythropoiesis for the detection of recombinant human erythropoietin abuse in athletes. Haematologica. 2000 Jun; 85(6):564-72.
  98. Iyer R, Baliga R, Nagel RL, Brugnara C, Kirchner K, Hogan S, Steinberg MH. Maximum urine concentrating ability in children with Hb SC disease: effects of hydroxyurea. Am J Hematol. 2000 May; 64(1):47-52.
  99. De Franceschi L, Fattovich G, Turrini F, Ayi K, Brugnara C, Manzato F, Noventa F, Stanzial AM, Solero P, Corrocher R. Hemolytic anemia induced by ribavirin therapy in patients with chronic hepatitis C virus infection: role of membrane oxidative damage. Hepatology. 2000 Apr; 31(4):997-1004.
  100. Brugnara C. Reticulocyte cellular indices: a new approach in the diagnosis of anemias and monitoring of erythropoietic function. Crit Rev Clin Lab Sci. 2000 Apr; 37(2):93-130.
  101. Donovan A, Brownlie A, Zhou Y, Shepard J, Pratt SJ, Moynihan J, Paw BH, Drejer A, Barut B, Zapata A, Law TC, Brugnara C, Lux SE, Pinkus GS, Pinkus JL, Kingsley PD, Palis J, Fleming MD, Andrews NC, Zon LI. Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter. Nature. 2000 Feb 17; 403(6771):776-81.
  102. De Franceschi L, Bachir D, Galacteros F, Tchernia G, Cynober T, Neuberg D, Beuzard Y, Brugnara C. Oral magnesium pidolate: effects of long-term administration in patients with sickle cell disease. Br J Haematol. 2000 Feb; 108(2):284-9.
  103. Su W, Shmukler BE, Chernova MN, Stuart-Tilley AK, de Franceschi L, Brugnara C, Alper SL. Mouse K-Cl cotransporter KCC1: cloning, mapping, pathological expression, and functional regulation. Am J Physiol. 1999 Nov; 277(5 Pt 1):C899-912.
  104. Gilligan DM, Lozovatsky L, Gwynn B, Brugnara C, Mohandas N, Peters LL. Targeted disruption of the beta adducin gene (Add2) causes red blood cell spherocytosis in mice. Proc Natl Acad Sci U S A. 1999 Sep 14; 96(19):10717-22.
  105. Socolovsky M, Fallon AE, Wang S, Brugnara C, Lodish HF. Fetal anemia and apoptosis of red cell progenitors in Stat5a-/-5b-/- mice: a direct role for Stat5 in Bcl-X(L) induction. Cell. 1999 Jul 23; 98(2):181-91.
  106. Brugnara C, Zurakowski D, DiCanzio J, Boyd T, Platt O. Reticulocyte hemoglobin content to diagnose iron deficiency in children. JAMA. 1999 Jun 16; 281(23):2225-30.
  107. Peters LL, Jindel HK, Gwynn B, Korsgren C, John KM, Lux SE, Mohandas N, Cohen CM, Cho MR, Golan DE, Brugnara C. Mild spherocytosis and altered red cell ion transport in protein 4. 2-null mice. J Clin Invest. 1999 Jun; 103(11):1527-37.
  108. Murad KL, Mahany KL, Brugnara C, Kuypers FA, Eaton JW, Scott MD. Structural and functional consequences of antigenic modulation of red blood cells with methoxypoly(ethylene glycol). Blood. 1999 Mar 15; 93(6):2121-7.
  109. Vitoux D, Beuzard Y, Brugnara C. The effect of hemoglobin A and S on the volume- and pH-dependence of K-Cl cotransport in human erythrocyte ghosts. J Membr Biol. 1999 Feb 1; 167(3):233-40.
  110. de Franceschi L, Shalev O, Piga A, Collell M, Olivieri O, Corrocher R, Hebbel RP, Brugnara C. Deferiprone therapy in homozygous human beta-thalassemia removes erythrocyte membrane free iron and reduces KCl cotransport activity. J Lab Clin Med. 1999 Jan; 133(1):64-9.
  111. Tanphaichitr VS, Sumboonnanonda A, Ideguchi H, Shayakul C, Brugnara C, Takao M, Veerakul G, Alper SL. Novel AE1 mutations in recessive distal renal tubular acidosis. Loss-of-function is rescued by glycophorin A. J Clin Invest. 1998 Dec 15; 102(12):2173-9.
  112. Brownlie A, Donovan A, Pratt SJ, Paw BH, Oates AC, Brugnara C, Witkowska HE, Sassa S, Zon LI. Positional cloning of the zebrafish sauternes gene: a model for congenital sideroblastic anaemia. Nat Genet. 1998 Nov; 20(3):244-50.
  113. Vandorpe DH, Shmukler BE, Jiang L, Lim B, Maylie J, Adelman JP, de Franceschi L, Cappellini MD, Brugnara C, Alper SL. cDNA cloning and functional characterization of the mouse Ca2+-gated K+ channel, mIK1. Roles in regulatory volume decrease and erythroid differentiation. J Biol Chem. 1998 Aug 21; 273(34):21542-53.
  114. Jarolim P, Shayakul C, Prabakaran D, Jiang L, Stuart-Tilley A, Rubin HL, Simova S, Zavadil J, Herrin JT, Brouillette J, Somers MJ, Seemanova E, Brugnara C, Guay-Woodford LM, Alper SL. Autosomal dominant distal renal tubular acidosis is associated in three families with heterozygosity for the R589H mutation in the AE1 (band 3) Cl-/HCO3- exchanger. J Biol Chem. 1998 Mar 13; 273(11):6380-8.
  115. Roback MG, Stack AM, Thompson C, Brugnara C, Schwarz HP, Saladino RA. Activated protein C concentrate for the treatment of meningococcal endotoxin shock in rabbits. Shock. 1998 Feb; 9(2):138-42.
  116. De Franceschi L, Cappellini MD, Graziadei G, Manzato F, Olivieri O, Corrocher R, Fiorelli G, Beuzard Y, Brugnara C. The effect of dietary magnesium supplementation on the cellular abnormalities of erythrocytes in patients with beta thalassemia intermedia. Haematologica. 1998 Feb; 83(2):118-25.
  117. Brugnara C. Use of reticulocyte cellular indices in the diagnosis and treatment of hematological disorders. Int J Clin Lab Res. 1998; 28(1):1-11.
  118. D'onofrio G, Zini G, Brugnara C. Clinical Applications of Automated Reticulocyte Indices. Hematology. 1998; 3(2):165-76.
  119. Rufo PA, Merlin D, Riegler M, Ferguson-Maltzman MH, Dickinson BL, Brugnara C, Alper SL, Lencer WI. The antifungal antibiotic, clotrimazole, inhibits chloride secretion by human intestinal T84 cells via blockade of distinct basolateral K+ conductances. Demonstration of efficacy in intact rabbit colon and in an in vivo mouse model of cholera. J Clin Invest. 1997 Dec 15; 100(12):3111-20.
  120. Madore F, Lowrie EG, Brugnara C, Lew NL, Lazarus JM, Bridges K, Owen WF. Anemia in hemodialysis patients: variables affecting this outcome predictor. J Am Soc Nephrol. 1997 Dec; 8(12):1921-9.
  121. De Franceschi L, Bachir D, Galacteros F, Tchernia G, Cynober T, Alper S, Platt O, Beuzard Y, Brugnara C. Oral magnesium supplements reduce erythrocyte dehydration in patients with sickle cell disease. J Clin Invest. 1997 Oct 1; 100(7):1847-52.
  122. Browne PV, Shalev O, Kuypers FA, Brugnara C, Solovey A, Mohandas N, Schrier SL, Hebbel RP. Removal of erythrocyte membrane iron in vivo ameliorates the pathobiology of murine thalassemia. J Clin Invest. 1997 Sep 15; 100(6):1459-64.
  123. Head CA, Brugnara C, Martinez-Ruiz R, Kacmarek RM, Bridges KR, Kuter D, Bloch KD, Zapol WM. Low concentrations of nitric oxide increase oxygen affinity of sickle erythrocytes in vitro and in vivo. J Clin Invest. 1997 Sep 1; 100(5):1193-8.
  124. Hansen A, Whitelaw A, Lapp C, Brugnara C. Cerebrospinal fluid plasminogen activator inhibitor-1: a prognostic factor in posthaemorrhagic hydrocephalus. Acta Paediatr. 1997 Sep; 86(9):995-8.
  125. Steinberg MH, Nagel RL, Brugnara C. Cellular effects of hydroxyurea in Hb SC disease. Br J Haematol. 1997 Sep; 98(4):838-44.
  126. Major A, Mathez-Loic F, Rohling R, Gautschi K, Brugnara C. The effect of intravenous iron on the reticulocyte response to recombinant human erythropoietin. Br J Haematol. 1997 Aug; 98(2):292-4.
  127. De Franceschi L, Brugnara C, Beuzard Y. Dietary magnesium supplementation ameliorates anemia in a mouse model of beta-thalassemia. Blood. 1997 Aug 1; 90(3):1283-90.
  128. Brugnara C, Zelmanovic D, Sorette M, Ballas SK, Platt O. Reticulocyte hemoglobin: an integrated parameter for evaluation of erythropoietic activity. Am J Clin Pathol. 1997 Aug; 108(2):133-42.
  129. Cazzola M, Mercuriali F, Brugnara C. Use of recombinant human erythropoietin outside the setting of uremia. Blood. 1997 Jun 15; 89(12):4248-67.
  130. Rittenhouse AR, Vandorpe DH, Brugnara C, Alper SL. The antifungal imidazole clotrimazole and its major in vivo metabolite are potent blockers of the calcium-activated potassium channel in murine erythroleukemia cells. J Membr Biol. 1997 May 15; 157(2):177-91.
  131. Magnani B, Fenton T, Lapp C, Brugnara C. Degree of agreement in plasma fibrinogen among two functional and one immunonephelometric assays. Am J Clin Pathol. 1997 May; 107(5):527-33.
  132. Brugnara C. Erythrocyte membrane transport physiology. Curr Opin Hematol. 1997 Mar; 4(2):122-7.
  133. Chan FY, Robinson J, Brownlie A, Shivdasani RA, Donovan A, Brugnara C, Kim J, Lau BC, Witkowska HE, Zon LI. Characterization of adult alpha- and beta-globin genes in the zebrafish. Blood. 1997 Jan 15; 89(2):688-700.
  134. Shmukler B, Sun T, Brugnara C, Alper SL. Reinterpretation of the RACTK1 K+ channel. Am J Physiol. 1997 Jan; 272(1 Pt 1):C350-4.
  135. Bridges KR, Barabino GD, Brugnara C, Cho MR, Christoph GW, Dover G, Ewenstein BM, Golan DE, Guttmann CR, Hofrichter J, Mulkern RV, Zhang B, Eaton WA. A multiparameter analysis of sickle erythrocytes in patients undergoing hydroxyurea therapy. Blood. 1996 Dec 15; 88(12):4701-10.
  136. Rufo PA, Jiang L, Moe SJ, Brugnara C, Alper SL, Lencer WI. The antifungal antibiotic, clotrimazole, inhibits Cl- secretion by polarized monolayers of human colonic epithelial cells. J Clin Invest. 1996 Nov 1; 98(9):2066-75.
  137. De Franceschi L, Beuzard Y, Jouault H, Brugnara C. Modulation of erythrocyte potassium chloride cotransport, potassium content, and density by dietary magnesium intake in transgenic SAD mouse. Blood. 1996 Oct 1; 88(7):2738-44.
  138. Peters LL, Shivdasani RA, Liu SC, Hanspal M, John KM, Gonzalez JM, Brugnara C, Gwynn B, Mohandas N, Alper SL, Orkin SH, Lux SE. Anion exchanger 1 (band 3) is required to prevent erythrocyte membrane surface loss but not to form the membrane skeleton. Cell. 1996 Sep 20; 86(6):917-27.
  139. Brugnara C, Gee B, Armsby CC, Kurth S, Sakamoto M, Rifai N, Alper SL, Platt OS. Therapy with oral clotrimazole induces inhibition of the Gardos channel and reduction of erythrocyte dehydration in patients with sickle cell disease. J Clin Invest. 1996 Mar 1; 97(5):1227-34.
  140. Armsby CC, Stuart-Tilley AK, Alper SL, Brugnara C. Resistance to osmotic lysis in BXD-31 mouse erythrocytes: association with upregulated K-Cl cotransport. Am J Physiol. 1996 Mar; 270(3 Pt 1):C866-77.
  141. de Franceschi L, Rouyer-Fessard P, Alper SL, Jouault H, Brugnara C, Beuzard Y. Combination therapy of erythropoietin, hydroxyurea, and clotrimazole in a beta thalassemic mouse: a model for human therapy. Blood. 1996 Feb 1; 87(3):1188-95.
  142. Coupry I, Armsby CC, Alper SL, Brugnara C, Parini A. Clotrimazole and efaroxan inhibit red cell Gardos channel independently of imidazoline I1 and I2 binding sites. Eur J Pharmacol. 1996 Jan 4; 295(1):109-12.
  143. De Franceschi L, Beuzard Y, Brugnara C. Sulfhydryl oxidation and activation of red cell K(+)-Cl- cotransport in the transgenic SAD mouse. Am J Physiol. 1995 Oct; 269(4 Pt 1):C899-906.
  144. Rifai N, Sakamoto M, Platt O, Brugnara C. A high-performance liquid chromatographic assay for the determination of itraconazole concentration using solid-phase extraction and small sample volume. Ther Drug Monit. 1995 Oct; 17(5):522-5.
  145. Coupry I, Armsby CC, Alper S, Parini A, Brugnara C. Inhibition of red cell Ca(2+)-activated K+ transport by clotrimazole does not take place via imidazoline binding sites. Ann N Y Acad Sci. 1995 Jul 12; 763:287-9.
  146. Brugnara C, De Franceschi L, Armsby CC, Saadane N, Trudel M, Beuzard Y, Rittenhouse A, Rifai N, Platt O, Alper SL. A new therapeutic approach for sickle cell disease. Blockade of the red cell Ca(2+)-activated K+ channel by clotrimazole. Ann N Y Acad Sci. 1995 Jul 12; 763:262-71.
  147. Benzaquen LR, Brugnara C, Byers HR, Gatton-Celli S, Halperin JA. Clotrimazole inhibits cell proliferation in vitro and in vivo. Nat Med. 1995 Jun; 1(6):534-40.
  148. Brugnara C, Armsby CC, Sakamoto M, Rifai N, Alper SL, Platt O. Oral administration of clotrimazole and blockade of human erythrocyte Ca(++)-activated K+ channel: the imidazole ring is not required for inhibitory activity. J Pharmacol Exp Ther. 1995 Apr; 273(1):266-72.
  149. Armsby CC, Brugnara C, Alper SL. Cation transport in mouse erythrocytes: role of K(+)-Cl- cotransport in regulatory volume decrease. Am J Physiol. 1995 Apr; 268(4 Pt 1):C894-902.
  150. Rifai N, Sakamoto M, Law T, Platt O, Mikati M, Armsby CC, Brugnara C. HPLC measurement, blood distribution, and pharmacokinetics of oral clotrimazole, potentially useful antisickling agent. Clin Chem. 1995 Mar; 41(3):387-91.
  151. Brugnara C. Erythrocyte dehydration in pathophysiology and treatment of sickle cell disease. Curr Opin Hematol. 1995 Mar; 2(2):132-8.
  152. Jarolim P, Rubin HL, Brabec V, Chrobak L, Zolotarev AS, Alper SL, Brugnara C, Wichterle H, Palek J. Mutations of conserved arginines in the membrane domain of erythroid band 3 lead to a decrease in membrane-associated band 3 and to the phenotype of hereditary spherocytosis. Blood. 1995 Feb 1; 85(3):634-40.
  153. Brugnara C, Hipp MJ, Irving PJ, Lathrop H, Lee PA, Minchello EM, Winkelman J. Automated reticulocyte counting and measurement of reticulocyte cellular indices. Evaluation of the Miles H*3 blood analyzer. Am J Clin Pathol. 1994 Nov; 102(5):623-32.
  154. Olivieri O, De Franceschi L, Capellini MD, Girelli D, Corrocher R, Brugnara C. Oxidative damage and erythrocyte membrane transport abnormalities in thalassemias. Blood. 1994 Jul 1; 84(1):315-20.
  155. Brugnara C, Laufer MR, Friedman AJ, Bridges K, Platt O. Reticulocyte hemoglobin content (CHr): early indicator of iron deficiency and response to therapy. Blood. 1994 May 15; 83(10):3100-1.
  156. Brugnara C, Colella GM, Cremins J, Langley RC, Schneider TJ, Rutherford CJ, Goldberg MA. Effects of subcutaneous recombinant human erythropoietin in normal subjects: development of decreased reticulocyte hemoglobin content and iron-deficient erythropoiesis. J Lab Clin Med. 1994 May; 123(5):660-7.
  157. Brugnara C, Fenton T, Winkelman JW. Management training for pathology residents. I. Results of a national survey. Am J Clin Pathol. 1994 May; 101(5):559-63.
  158. Winkelman JW, Brugnara C. Management training for pathology residents. II. Experience with a focused curriculum. Am J Clin Pathol. 1994 May; 101(5):564-8.
  159. De Franceschi L, Saadane N, Trudel M, Alper SL, Brugnara C, Beuzard Y. Treatment with oral clotrimazole blocks Ca(2+)-activated K+ transport and reverses erythrocyte dehydration in transgenic SAD mice. A model for therapy of sickle cell disease. J Clin Invest. 1994 Apr; 93(4):1670-6.
  160. Rutherford CJ, Schneider TJ, Dempsey H, Kirn DH, Brugnara C, Goldberg MA. Efficacy of different dosing regimens for recombinant human erythropoietin in a simulated perisurgical setting: the importance of iron availability in optimizing response. Am J Med. 1994 Feb; 96(2):139-45.
  161. Jarolim P, Rubin HL, Liu SC, Cho MR, Brabec V, Derick LH, Yi SJ, Saad ST, Alper S, Brugnara C, et al. Duplication of 10 nucleotides in the erythroid band 3 (AE1) gene in a kindred with hereditary spherocytosis and band 3 protein deficiency (band 3PRAGUE). J Clin Invest. 1994 Jan; 93(1):121-30.
  162. Brugnara C, de Franceschi L, Alper SL. Inhibition of Ca(2+)-dependent K+ transport and cell dehydration in sickle erythrocytes by clotrimazole and other imidazole derivatives. J Clin Invest. 1993 Jul; 92(1):520-6.
  163. Brugnara C, Kruskall MS, Johnstone RM. Membrane properties of erythrocytes in subjects undergoing multiple blood donations with or without recombinant erythropoietin. Br J Haematol. 1993 May; 84(1):118-30.
  164. Brugnara C. Membrane transport of Na and K and cell dehydration in sickle erythrocytes. Experientia. 1993 Feb 15; 49(2):100-9.
  165. Brugnara C, Chambers LA, Malynn E, Goldberg MA, Kruskall MS. Red blood cell regeneration induced by subcutaneous recombinant erythropoietin: iron-deficient erythropoiesis in iron-replete subjects. Blood. 1993 Feb 15; 81(4):956-64.
  166. Brugnara C, de Franceschi L. Effect of cell age and phenylhydrazine on the cation transport properties of rabbit erythrocytes. J Cell Physiol. 1993 Feb; 154(2):271-80.
  167. Goldberg MA, Brugnara C, Dover GJ, Schapira L, Lacroix L, Bunn HF. Hydroxyurea and erythropoietin therapy in sickle cell anemia. Semin Oncol. 1992 Jun; 19(3 Suppl 9):74-81.
  168. Brugnara C, Churchill WH. Effect of irradiation on red cell cation content and transport. Transfusion. 1992 Mar-Apr; 32(3):246-52.
  169. Churchill WH, Schmidt B, Lindsey J, Greenberg M, Boudrow S, Brugnara C. Thawing fresh frozen plasma in a microwave oven. A comparison with thawing in a 37 degrees C waterbath. Am J Clin Pathol. 1992 Feb; 97(2):227-32.
  170. Olivieri O, Vitoux D, Galacteros F, Bachir D, Blouquit Y, Beuzard Y, Brugnara C. Hemoglobin variants and activity of the (K+Cl-) cotransport system in human erythrocytes. Blood. 1992 Feb 1; 79(3):793-7.
  171. Brugnara C. [Physiopathology of erythrocyte membrane transport]. Haematologica. 1991 Jun; 76 Suppl 3:191-8.
  172. Goldberg MA, Brugnara C, Dover GJ, Schapira L, Charache S, Bunn HF. Treatment of sickle cell anemia with hydroxyurea and erythropoietin. N Engl J Med. 1990 Aug 9; 323(6):366-72.
  173. Halperin JA, Brugnara C, Van Ha T, Tosteson DC. Voltage-activated cation permeability in high-potassium but not low-potassium red blood cells. Am J Physiol. 1990 Jun; 258(6 Pt 1):C1169-72.
  174. Halperin JA, Brugnara C, Tosteson MT, Van Ha T, Tosteson DC. Voltage-activated cation transport in human erythrocytes. Am J Physiol. 1989 Nov; 257(5 Pt 1):C986-96.
  175. Brugnara C. Characteristics of the volume- and chloride-dependent K transport in human erythrocytes homozygous for hemoglobin C. J Membr Biol. 1989 Oct; 111(1):69-81.
  176. Brugnara C, Van Ha T, Tosteson DC. Acid pH induces formation of dense cells in sickle erythrocytes. Blood. 1989 Jul; 74(1):487-95.
  177. Brugnara C, Van Ha T, Tosteson DC. Role of chloride in potassium transport through a K-Cl cotransport system in human red blood cells. Am J Physiol. 1989 May; 256(5 Pt 1):C994-1003.
  178. Halperin JA, Brugnara C, Nicholson-Weller A. Ca2+-activated K+ efflux limits complement-mediated lysis of human erythrocytes. J Clin Invest. 1989 May; 83(5):1466-71.
  179. Brugnara C, Bunn HF, Tosteson DC. Ion content and transport and the regulation of volume in sickle cells. Ann N Y Acad Sci. 1989; 565:96-103.
  180. Brugnara C, Van Ha T, Tosteson DC. Properties of K+ transport in resealed human erythrocyte ghosts. Am J Physiol. 1988 Sep; 255(3 Pt 1):C346-56.
  181. Halperin JA, Nicholson-Weller A, Brugnara C, Tosteson DC. Complement induces a transient increase in membrane permeability in unlysed erythrocytes. J Clin Invest. 1988 Aug; 82(2):594-600.
  182. Brugnara C, Tosteson DC. Inhibition of K transport by divalent cations in sickle erythrocytes. Blood. 1987 Dec; 70(6):1810-5.
  183. Canessa M, Brugnara C, Escobales N. The Li+-Na+ exchange and Na+-K+-Cl- cotransport systems in essential hypertension. Hypertension. 1987 Nov; 10(5 Pt 2):I4-10.
  184. Corrocher R, Ferrari S, Bassi A, Guarini P, Bertinato L, Olivieri O, Licia GM, Ruzzenente O, Brugnara C, De Sandre G. Membrane polyunsaturated fatty acids and lithium-sodium countertransport in human erythrocytes. Life Sci. 1987 Aug 31; 41(9):1171-8.
  185. Halperin JA, Brugnara C, Kopin AS, Ingwall J, Tosteson DC. Properties of the Na+-K+ pump in human red cells with increased number of pump sites. J Clin Invest. 1987 Jul; 80(1):128-37.
  186. Brugnara C, Tosteson DC. Cell volume, K transport, and cell density in human erythrocytes. Am J Physiol. 1987 Mar; 252(3 Pt 1):C269-76.
  187. Brock TA, Brugnara C, Canessa M, Gimbrone MA. Bradykinin and vasopressin stimulate Na+-K+-Cl- cotransport in cultured endothelial cells. Am J Physiol. 1986 Jun; 250(6 Pt 1):C888-95.
  188. Brugnara C, Bunn HF, Tosteson DC. Regulation of erythrocyte cation and water content in sickle cell anemia. Science. 1986 Apr 18; 232(4748):388-90.
  189. Corrocher R, Bonfanti F, Steinmayr M, Brugnara C, Bertinato L, De Sandre G. [Frequency and sex and age distribution of cardiopathy in arterial hypertension]. Minerva Cardioangiol. 1986 Mar; 34(3):119-25.
  190. Bonfanti F, Corrocher R, Bertinato L, Brugnara C, Nortilli R, De Sandre G. [Incidence of retinal and renal complications in arterial hypertension]. Minerva Cardioangiol. 1986 Mar; 34(3):99-105.
  191. Canessa M, Brugnara C, Cusi D, Tosteson DC. Modes of operation and variable stoichiometry of the furosemide- sensitive Na and K fluxes in human red cells. J Gen Physiol. 1986 Jan; 87(1):113-42.
  192. Brugnara C, Canessa M, Cusi D, Tosteson DC. Furosemide-sensitive Na and K fluxes in human red cells. Net uphill Na extrusion and equilibrium properties. J Gen Physiol. 1986 Jan; 87(1):91-112.
  193. Brugnara C, Kopin AS, Bunn HF, Tosteson DC. Regulation of cation content and cell volume in hemoglobin erythrocytes from patients with homozygous hemoglobin C disease. J Clin Invest. 1985 May; 75(5):1608-17.
  194. Corrocher R, Steinmayr M, Ruzzenente O, Brugnara C, Bertinato L, Mazzi M, Furri C, Bonfanti F, De Sandre G. Elevation of red cell sodium-lithium countertransport in hyperlipidemias. Life Sci. 1985 Feb 18; 36(7):649-55.
  195. Canessa M, Brugnara C. The regulation of Na-K cotransport system and essential hypertension. Klin Wochenschr. 1985; 63 Suppl 3:52-4.
  196. Dagher G, Brugnara C, Canessa M. Effect of metabolic depletion on the furosemide-sensitive Na and K fluxes in human red cells. J Membr Biol. 1985; 86(2):145-55.
  197. Corrocher R, Brugnara C, Maso R, Taddei G, Mezzelani P, De Sandre G. Multiple arterial stenoses in chronic ergot toxicity. N Engl J Med. 1984 Jan 26; 310(4):261.
  198. Brugnara C, Kopin AS, Bunn HF, Tosteson DC. Electrolyte composition and equilibrium in hemoglobin CC red blood cells. Trans Assoc Am Physicians. 1984; 97:104-12.
  199. Brugnara C, Corrocher R, Foroni L, Steinmayr M, Bonfanti F, De Sandre G. Lithium-sodium countertransport in erythrocytes of normal and hypertensive subjects. Relationship with age and plasma renin activity. Hypertension. 1983 Jul-Aug; 5(4):529-34.
  200. Bonfanti F, Pecci R, Nortilli R, Brugnara C, Corrocher R. [Relations between renin activity and vascular complications of arterial hypertension]. Minerva Cardioangiol. 1982 Mar; 30(3):75-80.
  201. De Franceschi L, Franco RS, Bertoldi M, Brugnara C, Matté A, Siciliano A, Wieschhaus AJ, Chishti AH, Joiner CH. Pharmacological inhibition of calpain-1 prevents red cell dehydration and reduces Gardos channel activity in a mouse model of sickle cell disease. FASEB J. 2013 Feb; 27(2):750-9.
  202. Brugnara C, De Franceschi L, Alper SL. Ca(2+)-activated K+ transport in erythrocytes. Comparison of binding and transport inhibition by scorpion toxins. J Biol Chem. 1993 Apr 25; 268(12):8760-8.
  203. Marinkovic D, Zhang X, Yalcin S, Luciano JP, Brugnara C, Huber T, Ghaffari S. Foxo3 is required for the regulation of oxidative stress in erythropoiesis. J Clin Invest. 2007 Aug; 117(8):2133-44.
  204. Rivera A, Jarolim P, Brugnara C. Modulation of Gardos channel activity by cytokines in sickle erythrocytes. Blood. 2002 Jan 1; 99(1):357-603.
  205. Shmukler BE, Brugnara C, Alper SL. Structure and genetic polymorphism of the mouse KCC1 gene. Biochim Biophys Acta. 2000 Jul 24; 1492(2-3):353-61.
  206. De Franceschi L, Brugnara C, Rouyer-Fessard P, Jouault H, Beuzard Y. Formation of dense erythrocytes in SAD mice exposed to chronic hypoxia: evaluation of different therapeutic regimens and of a combination of oral clotrimazole and magnesium therapies. Blood. 1999 Dec 15; 94(12):4307-13.
  207. Bonfanti F, Corrocher R, Cherubini M, Brugnara C, Fugazzola C, Frasson F. [Multiple renal arteries and arterial hypertension: a review of 337 renal arteriography cases]. Minerva Cardioangiol. 1980 May; 28(5):289-04.
  208. Stocker JW, De Franceschi L, McNaughton-Smith GA, Corrocher R, Beuzard Y, Brugnara C. ICA-17043, a novel Gardos channel blocker, prevents sickled red blood cell dehydration in vitro and in vivo in SAD mice. Blood. 2003 Mar 15; 101(6):2412-8.
  209. Rivera A, Rotter MA, Brugnara C. Endothelins activate Ca(2+)-gated K(+) channels via endothelin B receptors in CD-1 mouse erythrocytes. Am J Physiol. 1999 Oct; 277(4 Pt 1):C746-54.
  210. Rivera A, Ferreira A, Bertoni D, Romero JR, Brugnara C. Abnormal regulation of Mg2+ transport via Na/Mg exchanger in sickle erythrocytes. Blood. 2005 Jan 1; 105(1):382-6.
  211. De Franceschi L, Biondani A, Carta F, Turrini F, Laudanna C, Deana R, Brunati AM, Turretta L, Iolascon A, Perrotta S, Elson A, Bulato C, Brugnara C. PTPepsilon has a critical role in signaling transduction pathways and phosphoprotein network topology in red cells. Proteomics. 2008 Nov; 8(22):4695-708.
  212. Andolfo I, Alper SL, Delaunay J, Auriemma C, Russo R, Asci R, Esposito MR, Sharma AK, Shmukler BE, Brugnara C, De Franceschi L, Iolascon A. Missense mutations in the ABCB6 transporter cause dominant familial pseudohyperkalemia. Am J Hematol. 2013 Jan; 88(1):66-72.
  213. Bize I, Güvenç B, Robb A, Buchbinder G, Brugnara C. Serine/threonine protein phosphatases and regulation of K-Cl cotransport in human erythrocytes. Am J Physiol. 1999 Nov; 277(5 Pt 1):C926-36.
  214. Casula S, Zolotarev AS, Stuart-Tilley AK, Wilhelm S, Shmukler BE, Brugnara C, Alper SL. Chemical crosslinking studies with the mouse Kcc1 K-Cl cotransporter. Blood Cells Mol Dis. 2009 May-Jun; 42(3):233-40.
  215. Rittenhouse AR, Parker C, Brugnara C, Morgan KG, Alper SL. Inhibition of maxi-K currents in ferret portal vein smooth muscle cells by the antifungal clotrimazole. Am J Physiol. 1997 Jul; 273(1 Pt 1):C45-56.
  216. Brugnara C, Armsby CC, De Franceschi L, Crest M, Euclaire MF, Alper SL. Ca(2+)-activated K+ channels of human and rabbit erythrocytes display distinctive patterns of inhibition by venom peptide toxins. J Membr Biol. 1995 Sep; 147(1):71-82.
  217. De Franceschi L, Platt OS, Malpeli G, Janin A, Scarpa A, Leboeuf C, Beuzard Y, Payen E, Brugnara C. Protective effects of phosphodiesterase-4 (PDE-4) inhibition in the early phase of pulmonary arterial hypertension in transgenic sickle cell mice. FASEB J. 2008 Jun; 22(6):1849-60.
  218. de Franceschi L, Malpeli G, Scarpa A, Janin A, Muchitsch EM, Roncada P, Leboeuf C, Corrocher R, Beuzard Y, Brugnara C. Protective effects of S-nitrosoalbumin on lung injury induced by hypoxia-reoxygenation in mouse model of sickle cell disease. Am J Physiol Lung Cell Mol Physiol. 2006 Sep; 291(3):L457-65.
  219. De Franceschi L, Villa-Moruzzi E, Fumagalli L, Brugnara C, Turrini F, Motta R, Veghini E, Corato C, Alper SL, Berton G. K-Cl cotransport modulation by intracellular Mg in erythrocytes from mice bred for low and high Mg levels. Am J Physiol Cell Physiol. 2001 Oct; 281(4):C1385-95.
  220. Danise P, Amendola G, Nobili B, Perrotta S, Miraglia Del Giudice E, Matarese SM, Iolascon A, Brugnara C. Flow-cytometric analysis of erythrocytes and reticulocytes in congenital dyserythropoietic anaemia type II (CDA II): value in differential diagnosis with hereditary spherocytosis. Clin Lab Haematol. 2001 Feb; 23(1):7-13.
  221. Wieschhaus A, Khan A, Zaidi A, Rogalin H, Hanada T, Liu F, De Franceschi L, Brugnara C, Rivera A, Chishti AH. Calpain-1 knockout reveals broad effects on erythrocyte deformability and physiology. Biochem J. 2012 Nov 15; 448(1):141-52.
  222. Alper SL, Vandorpe DH, Peters LL, Brugnara C. Reduced DIDS-sensitive chloride conductance in Ae1-/- mouse erythrocytes. Blood Cells Mol Dis. 2008 Jul-Aug; 41(1):22-34.
  223. Abdulmalik O, Safo MK, Chen Q, Yang J, Brugnara C, Ohene-Frempong K, Abraham DJ, Asakura T. 5-hydroxymethyl-2-furfural modifies intracellular sickle haemoglobin and inhibits sickling of red blood cells. Br J Haematol. 2005 Feb; 128(4):552-61.
  224. Andolfo I, Alper SL, De Franceschi L, Auriemma C, Russo R, De Falco L, Vallefuoco F, Esposito MR, Vandorpe DH, Shmukler BE, Narayan R, Montanaro D, D'Armiento M, Vetro A, Limongelli I, Zuffardi O, Glader BE, Schrier SL, Brugnara C, Stewart GW, Delaunay J, Iolascon A. Multiple clinical forms of dehydrated hereditary stomatocytosis arise from mutations in PIEZO1. Blood. 2013 May 9; 121(19):3925-35, S1-12.
  225. Franco SS, De Falco L, Ghaffari S, Brugnara C, Sinclair DA, Matte' A, Iolascon A, Mohandas N, Bertoldi M, An X, Siciliano A, Rimmelé P, Cappellini MD, Michan S, Zoratti E, Anne J, De Franceschi L. Resveratrol accelerates erythroid maturation by activation of FoxO3 and ameliorates anemia in beta-thalassemic mice. Haematologica. 2014 Feb; 99(2):267-75.
  226. Darbari DS, Nouraie M, Taylor JG, Brugnara C, Castro O, Ballas SK. Alpha-thalassaemia and response to hydroxyurea in sickle cell anaemia. Eur J Haematol. 2014 Apr; 92(4):341-5.
  227. Zhang X, Campreciós G, Rimmelé P, Liang R, Yalcin S, Mungamuri SK, Barminko J, D'Escamard V, Baron MH, Brugnara C, Papatsenko D, Rivella S, Ghaffari S. FOXO3-mTOR metabolic cooperation in the regulation of erythroid cell maturation and homeostasis. Am J Hematol. 2014 Oct; 89(10):954-63.
  228. Archer NM, Shmukler BE, Andolfo I, Vandorpe DH, Gnanasambandam R, Higgins JM, Rivera A, Fleming MD, Sachs F, Gottlieb PA, Iolascon A, Brugnara C, Alper SL, Nathan DG. Hereditary xerocytosis revisited. Am J Hematol. 2014 Dec; 89(12):1142-6.
  229. Egan ES, Jiang RH, Moechtar MA, Barteneva NS, Weekes MP, Nobre LV, Gygi SP, Paulo JA, Frantzreb C, Tani Y, Takahashi J, Watanabe S, Goldberg J, Paul AS, Brugnara C, Root DE, Wiegand RC, Doench JG, Duraisingh MT. Malaria. A forward genetic screen identifies erythrocyte CD55 as essential for Plasmodium falciparum invasion. Science. 2015 May 8; 348(6235):711-4.
  230. Kalish BT, Matte A, Andolfo I, Iolascon A, Weinberg O, Ghigo A, Cimino J, Siciliano A, Hirsch E, Federti E, Puder M, Brugnara C, De Franceschi L. Dietary ?-3 fatty acids protect against vasculopathy in a transgenic mouse model of sickle cell disease. Haematologica. 2015 Jul; 100(7):870-80.
  231. Boscolo E, Limaye N, Huang L, Kang KT, Soblet J, Uebelhoer M, Mendola A, Natynki M, Seront E, Dupont S, Hammer J, Legrand C, Brugnara C, Eklund L, Vikkula M, Bischoff J, Boon LM. Rapamycin improves TIE2-mutated venous malformation in murine model and human subjects. J Clin Invest. 2015 Sep; 125(9):3491-504.
  232. Tubman VN, Mejia P, Shmukler BE, Bei AK, Alper SL, Mitchell JR, Brugnara C, Duraisingh MT. The Clinically Tested Gardos Channel Inhibitor Senicapoc Exhibits Antimalarial Activity. Antimicrob Agents Chemother. 2015 Oct 12; 60(1):613-6.
  233. Giani FC, Fiorini C, Wakabayashi A, Ludwig LS, Salem RM, Jobaliya CD, Regan SN, Ulirsch JC, Liang G, Steinberg-Shemer O, Guo MH, Esko T, Tong W, Brugnara C, Hirschhorn JN, Weiss MJ, Zon LI, Chou ST, French DL, Musunuru K, Sankaran VG. Targeted Application of Human Genetic Variation Can Improve Red Blood Cell Production from Stem Cells. Cell Stem Cell. 2016 Jan 7; 18(1):73-8.
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