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Children's Hospital Research Children's Hospital Labs
The Stem Cell Program
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Stem Cell Program Labs: Research Underway

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The hESC Core Facility at Children's Hospital Boston
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The hESC Core Facility at Children's Hospital Boston provides services to the Children's stem cell community (training in stem cell culture, regulations, and separation of funding requirements). We also maintain the internal Children's stem cell registry and bank, and expand, QC, and ship Children's hESC lines to laboratories interested in these lines.
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Our research efforts focus on directed differentiation of pluripotent stem cells. We combine fluorescent imaging and other stem cell based assays with high-throughput chemical screening approaches in order to identify novel agents and pathways that affect cell fate. Our goal is to derive blood stem cells from pluripotent stem cells.
Lab Members
Lab Members
Thorsten Schlaeger PI schlaeger@enders.tch.harvard.edu
Xiao Guan Postdoc guan@enders.tch.harvard.edu
Odelya Hartung Res. Assist. ohartung@enders.tch.harvard.edu
Phil Manos Res. Assist. pmanos@enders.tch.harvard.edu
Iris Huo Res. Assist. Hongguang.Huo@childrens.harvard.edu
Mam Ratanasirintrawoot PhD Student Ratanas@fas.harvard.edu
Sumon Datta former Res. Assist. (now at Novartis/NIBR)
Justine Miller former Res. Assist. (now PhD Student @ MSKCC)
Leah Boyer former Res. Assist. (now PhD Student @ UCSD)
Holly Young former Res. Assist. (now @ CyThera/Novocell)
Recent Publications

Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells. Chan EM, Ratanasirintrawoot S, Park IH, Manos PD, Loh YH, Huo H, Miller JD, Hartung O, Rho J, Ince TA, Daley GQ, Schlaeger TM. Nat Biotechnol. 2009 Oct 11.

Improved electrospray ionization efficiency compensates for diminished chromatographic resolution and enables proteomics analysis of tyrosine signaling in embryonic stem cells. Ficarro SB, Zhang Y, Lu Y, Moghimi AR, Askenazi M, Hyatt E, Smith ED, Boyer L, Schlaeger TM, Luckey CJ, Marto JA. Anal Chem. 2009 May 1;81(9):3440-7.

Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos. Lerou PH, Yabuuchi A, Huo H, Miller JD, Boyer LF, Schlaeger TM, Daley GQ. Nat Protoc. 2008;3(5):923-33.

Inducible endothelial cell-specific gene expression in transgenic mouse embryos and adult mice. Deutsch U, Schlaeger TM, Dehouck B, Döring A, Tauber S, Risau W, Engelhardt B. Exp Cell Res. 2008 Apr 1;314(6):1202-16.

Enhanced plating efficiency of trypsin-adapted human embryonic stem cells is reversible and independent of trisomy 12/17. Chan EM, Yates F, Boyer LF, Schlaeger TM, Daley GQ. Cloning Stem Cells. 2008 Mar;10(1):107-18.

Science aside: the trajectory of embryonic stem cell research in the USA. Schlaeger TM, Lensch MW, Taylor PL. Drug Discov Today. 2007 Apr;12(7-8):269-71.

Teratoma formation assays with human embryonic stem cells: a rationale for one type of human-animal chimera. Lensch MW, Schlaeger TM, Zon LI, Daley GQ. Cell Stem Cell. 2007 Sep 13;1(3):253-8.

Pluripotent stem cells and their niches. Lensch MW, Daheron L, Schlaeger TM. Stem Cell Rev. 2006;2(3):185-201. Review.
Available hESC Lines
code type sex karyotype P origin reference
CHB2 hESC F normal 24 discarded, poor quality, fresh embryo (co-culture of 5 non-blastocyst) PMID: 18223642
CHB4 hESC M normal 19 discarded, poor quality, fresh embryo (blastocysts) PMID: 18223642
CHB6 hESC F normal 26 discarded, poor quality, fresh embryo (blastocysts) PMID: 18223642
CHB8 hESC F normal 23 discarded, poor quality, fresh embryo (blastocysts) PMID: 18223642
CHB10 hESC M normal 20 discarded, poor quality, fresh embryo (blastocysts) PMID: 18223642
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