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Department
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Pathology
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Hospital Title
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Director of the Proteomics Center
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Academic Title
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Assistant Professor, Department of Pathology
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Phone
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+1 (617) 919-2629
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Fax
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+1 (617) 730-0168
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Email
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Hanno Steen
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Location
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320 Longwood Avenue
Boston MA 02115
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Hanno Steen is a pioneer in developing methods for the qualitative and quantitative analysis of protein modifications by mass spectrometry, with special emphasis on tyrosine phosphorylation. In carcinogenesis, the pivotal role of tyrosine phosphorylation is underscored by the notion that almost half of the oncogenes known to date encode tyrosine kinases.
Hanno Steen's interests are focused on proteomics and biological mass spectrometry in general. He is particularly interested in the mass spectrometric analysis of the composition of complex protein mixtures, which includes not only the cataloging but also the comprehensive characterization of the components.
Dr. Steen's lab is working on novel methods for identifying and quantifying various protein modifications that are involved in pediatric diseases in order to provide new insights into the underlying biological processes, a prerequisite for a rational approach to prevent and/or cure these diseases.
Another goal of the lab's research is the development and applications of screening methods to detect and identify diagnostic and prognostic disease markers in body fluids, which offer an easily accessible mirror of the state of the entire body. These biomarkers can guide treatment decisions, predict patient phenotypes, and allow for early diagnosis.
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Dr. Steen studied chemistry at the University in Freiburg (Germany), UMIST/Manchester (UK) and Swiss Federal Institute of Technology (ETH) Zurich (Switzerland). He received his PhD in Biochemistry and Molecular Biology from the University of Southern Denmark working in the laboratory of Matthias Mann on the mass spectrometric analysis of protein modifications. He completed postdoctoral fellowships at the Departments of Cell Biology and Systems Biology at Harvard Medical School.
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- Steen, H., Kuster, B., Fernandez, M., Pandey, A., and Mann, M. (2001). Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode. Anal Chem 73, 1440-1448.
- Steen, H., Fernandez, M., Ghaffari, S., Pandey, A., and Mann, M. (2003). Phosphotyrosine Mapping in Bcr/Abl Oncoprotein Using Phosphotyrosine-specific Immonium Ion Scanning. Mol Cell Proteomics 2, 138-145.
- Steen, H., and Mann, M. (2004). The abc's (and xyz's) of peptide sequencing. Nat Rev Mol Cell Biol 5, 699-711.
- Steen, H., Jebanathirajah, J., Springer, M., and Kirschner, M. W. (2005). Stable Isotope-free Relative and Absolute Quantitation of Protein Phosphorylation by Mass Spectrometry. Proc Natl Acad Sci USA 102, 3948-3953.
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