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Department
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Genomics
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Hospital Title
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Assistant in Medicine
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Academic Title
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Assistant Professor of Pediatrics
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Phone
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617-919-2152
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Fax
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617-730-0253
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Email
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Emanuela Gussoni
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Location
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300 Longwood Avenue
Enders-5
Boston MA 02115
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The Gussoni laboratory's long-term goal is to optimize a cell-based therapy for patients with muscular dystrophy. Dr. Gussoni and colleagues are now pursuing this goal by optimizing human muscle stem cell isolation and characterization, as well as by creating new animal models for preclinical testing. They also are thoroughly comparing the transcriptome--the set of active genes--of human muscle stem cells to that of committed muscle cells using microarray technology. These studies are expected to lead to the identification of specific muscle stem cell markers that will make it easier to purify stem cells from primary tissues.
Down the road, the group seeks to identify the mechanisms by which muscle stem cells fuse to pre-existing myofibers and to optimize the injection of human muscle stem cells in immunocompromised mice with muscular dystrophy.
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Emanuela Gussoni received her PhD from the University of Milan, Italy and continued her postdoctoral training at Stanford University from 1990-1994 and at Children's Hospital Boston in the laboratory of Dr. Louis Kunkel from 1994-1999.
To view Dr. Gussoni's publications via PubMed Click Here.
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- Gussoni E, Soneoka Y, Strickland CD, Buzney EB, Flint AF, Khan MK, Kunkel LM, Mulligan RC. Dystrophin expression in the mdx mouse restored by stem cell transplantation. Nature 1999; 401: 390-394.
- Gussoni E, Bennett RR, Muskiewicz KR, Meyerrose T, Nolta JA, Gilgoff I, Stein J, Chan, YM, Lidov HG, Bönnemann CG, von Moers A, Morris GE, den Dunnen JT, Chamberlain JS, Kunkel LM and Weinberg K. Long-term persistence of donor nuclei in a Duchenne muscular dystrophy patient receiving bone marrow transplantation. Journal of Clinical Investigation 2002; 110: 807-814.
- Rivier F, Alkan O, Flint A, Muskiewicz K, Allen PD, Leboulch P and Gussoni E. Role of bone marrow cell trafficking in replenishing skeletal muscle SP and MP cell populations. Journal of Cell Science 2004; 117: 1979-1988.
- Montanaro F, Liadaki K, Schienda J, Flint A, Gussoni E, Kunkel LM. Demystifying SP cell purification: viability, yield, and phenotype are defined by isolation parameters. Exp Cell Res. 2004 298(1):144-54.
- Pavlath GK, Gussoni E. Human myoblasts and muscle-derived SP cells. Methods Mol Med. 2005 107:97-110.
- Muskiewicz KR, Frank NY, Flint AF, Gussoni E. Myogenic potential of muscle side and main population cells after intravenous injection into sub-lethally irradiated mdx mice. J Histochem Cytochem. 2005 53(7):861-73.
- Cerletti M, Molloy MJ, Tomczak KK, Yoon S, Ramoni MF, Kho AT, Beggs AH, Gussoni E. Melanoma cell adhesion molecule is a novel marker for human fetal myogenic cells and affects myoblast fusion. J Cell Sci. 2006 119(Pt 15):3117-27. Epub 2006 Jul 11.
- Frank NY, Kho AT, Schatton T, Murphy GF, Molloy MJ, Zhan Q, Ramoni MF, Frank MH, Kohane IS, Gussoni E. Regulation of myogenic progenitor proliferation in human fetal skeletal muscle by BMP4 and its antagonist Gremlin. J Cell Biol. 2006 175(1):99-110. Epub 2006 Oct 2.
- Yoon S, Molloy MJ, Wu MP, Cowan DB, Gussoni, E. C6ORF32 is upregulated during muscle cell differentiation and induces the formation of cellular filopodia. Dev Biol. 2007 301(1):70-81. Epub 2006 Nov 7
- Peault B, Rudnicki M, Torrente Y, Cossu G, Tremblay JP, Patridge T, Gussoni E, Kunkel LM, Huard J. Stem and Progenitor Cells in Skeletal Muscle Development, Maintenance, and Therapy. Mol. Ther. 2007 15(5): 867-877
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