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Center for Molecular Orthopedics
Chris Evans, PhD (Principal Investigator)
Ryan Porter, PhD; Fangjun Liu, PhD
Carmencita Pilapil, Brigham and Women's
Andre Steinert, MD; Orthopaedic Instititute, Koenig-Ludwig-Haus
Julius-Maximilians-University Wuerzburg
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The Tabin Lab
Richard Pearse, PhD
Cliff Tabin, PhD,
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In collaboration with Cliff Tabin, PhD and Richard Pearse, PhD, we are working to identify unique cell markers for anterior cruciate ligament cells, specifically markers that are different in tendon and ligament fibroblasts.
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With the help of collaborators in the Center for Molecular Orthopaedics, we are working to:
- identify subpopulations of cells within the anterior cruciate ligament (ACL), and
- identify the responses of these subpopulations of cells to manipulations of gene expression in an effort to promote cellular responses favorable for accelerated wound healing.
Work has been aimed at shaping gene expression in a collagen hydrogel microenvironment through viral vector delivery of transgenes(1) and siRNAs (Fig. 1).
Strategies to augment the quantity of mesenchymal stem cells present in the wound site are also being pursued.
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| click for larger image |
Fig. 1: Successful siRNA suppression of GFP in primary ACL cells. Cells were first infected with
oligofectamine used to transfect selected wells with the siRNA for GFP. 10A is the flow cytometry data, while 10B has the corresponding fluorescence.
1. Control (no GFP, no siRNA)
2. GFP at 10 MOI, no siRNA
3. GFP at 100 MOI, no siRNA
4. GFP at 300 MOI, no siRNA
5. GFP at 10 MOI, with siRNA
6. GFP at MOI 100, with siRNA
7. GFP at MOI 300, with siRNA
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1. Pascher A, Steinert AF, Palmer GD, Betz O, Gouze JN, Gouze E, Pilapil C, Ghivizzani SC, Evans CH, Murray MM: Enhanced repair of the anterior cruciate ligament by in situ gene transfer: evaluation in an in vitro model. Mol Ther 10:327-36, 2004
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