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Center Guidelines
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If you are a first time user, if you are interested in the analysis of protein modifications, and/or if you want to submit a large number of samples (> 20), please contact the staff of the Proteomics Center prior to sample submission.
No radioactive samples will be accepted.
We strongly recommend reduction and alkylation of samples prior to separation by SDS-PAGE. Protocols can be found here.
Although we do analyze silver-stained samples, we recommend providing 'Coomassie-stainable' amounts of protein. If protein modification analysis is the aim, we only accept Coomassie-stained bands.
If silver staining methods are applied please ensure that mass spectrometry compatible protocols are used. Protocols can be found here.
When excising the gel band/spot of interest, cut as close to the edge of the band as possible in order to reduce the amount of 'background' gel.
Please provide a gel image of the gel with indications of the excised band(-s).
Place the gel band/plug into a plain 1.5mL Eppendorf tube and store them covered with water in the freezer; shipping/storing on dry ice is not necessary.
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