Clinical Flow Cytometry Lab

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Contact the Department of Pathology

  • 617-355-7431
The Clinical Flow Cytometry Laboratory is committed to providing the highest quality clinical flow cytometry assays to aid in the diagnosis and treatment of oncologic, hematologic and immunologic disorders. We are a full service CLIA certified laboratory that offers a comprehensive test menu for the diagnosis of all types of leukemia, lymphomas and related hematological disorders. Our instruments include two BD FACSCanto II systems with three lasers to detect up to eight different fluorochromes.

Flow cytometry can be performed on peripheral blood, bone marrow, miscellaneous body fluids, and cells derived from lymph nodes and solid tumors. Please print a Clinical Flow Cytometry lab form, fill it out fully, and send it to the below address with your specimens. Please contact us if you have any questions.

Medical Director for Immunology: Francisco A. Bonilla, MD., PhD.

Medical Director for Leukemia/Lymphoma: Olga K. Weinberg, MD.

Laboratory Scientist: Erdyni N. Tsitsikov, PhD.

Turnaround time: Turnaround time for all tests, except proliferation, is 1 to 2 days. For proliferation it is 4 to 7 days.

Lab hours: The lab is open 7 days a week 7 AM to 5 PM (EST)

Tests offered:
Immunophenotyping
Acute Myeloid Leukemia (AML) Panel
AML Minimal Residual Disease
B cell Acute Lymphoblastic Leukemia (ALL) Panel
ALL Minimal Residual Disease
Leukemia Cell Ploidy
Lymphoma Panel (4 or 7 color)
Myelodysplastic Syndrome (MDS) panel
T cell Acute Lymphoblastic Leukemia Panel

Immune System Status
Acute Lymphoproliferative Syndrome (ALPS and gd T cells)
B cell Number (Total B Lymphocyte Count)
CD40 Ligand (CD154)
Common Variable Immunodeficiency (CVID)
IL-2 Receptor gamma Chain (CD132)
Leukocyte Adhesion Deficiency 1 &2 (LAD)
Memory B cells (IgD vs CD27)
Memory T cells (CD45RA vs CCR7)
Neutrophil Oxidative Burst (DHR)
Recent Thymic Emigrants (RTE: CD45RA vs CD31)
Regulatory T cells (Tregs: CD25 vs CD127)
SCID panel (T/B subsets & Memory T cells)
T/B Subsets (TBNK: Absolute Lymphocyte Counts)
T cell Mitogen Proliferation (Con A, PHA, anti-CD3)
T cell Antigen Proliferation (Tetanus, Candida)

Contact Information:
Clinical Flow Cytometry Laboratory
Department of Laboratory Medicine
Boston Children’s Hospital
300 Longwood Avenue, Bader 773
Boston, MA 02115

Phone: 617-355-7620
Fax: 617-730-0383
Email: ClinicalFlowCytometry@childrens.harvard.edu
We are grateful to have been ranked #1 on U.S. News & World Report's list of the best children's hospitals in the nation for the third year in a row, an honor we could not have achieved without the patients and families who inspire us to do our very best for them. Thanks to you, Boston Children's is a place where we can write the greatest children's stories ever told.”
- Sandra L. Fenwick, President and CEO

Boston Children's Hospital 300 Longwood Avenue, Boston, MA 02115 617-355-6000 | 800-355-7944

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